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The map label for this gene is htrB

Identifier: 30995456

GI number: 30995456

Start: 1597280

End: 1598215

Strand: Direct

Name: htrB

Synonym: HI1527

Alternate gene names: 30995456

Gene position: 1597280-1598215 (Clockwise)

Preceding gene: 16273424

Following gene: 16273428

Centisome position: 87.28

GC content: 40.49

Gene sequence:

>936_bases
ATGAAAAACGAAAAACTCCCTCAATTTCAACCGCACTTTTTAGCCCCAAAATACTGGCTTTTTTGGCTAGGCGTGGCAAT
TTGGCGAAGTATTTTATGTCTTCCCTATCCTATTTTGCGCCATATTGGTCATGGTTTCGGTTGGCTGTTTTCACATTTAA
AAGTGGGTGAACGTCGAGCTGCCATTGCACGCCGTAATCTTGAACTTTGTTTCCCAGACATGCCAGAGAATGAACGTGAG
GTAATTTTGCAAGAAAATCTCCGTTCAGTAGGCATGGCAATTATCGAAACTGGCATGGCTTGGTTTTGGTCGGATTCACG
TATCAAAAAATGGTCGAAAGTTGAAGGCTTACATTATCTAAAAGAAAATCAAAAAGATGGAATTGTTCTCGTCGGTGTTC
ATTTCTTAACGCTAGAACTTGGCGCTCGAATCATTGGTTTGCATCATCCTGGCATTGGTGTTTATCGTCCAAATGATAAT
CCTTTGCTTGATTGGCTACAAACGCAAGGTCGTTTACGCTCTAATAAAGATATGCTTGATCGTAAAGATTTACGCGGAAT
GATCAAAGCTTTACGCCACGAAGAAACCATTTGGTATGCGCCTGATCACGATTACGGCAGAAAAAATGCCGTTTTTGTTC
CTTTTTTTGCAGTACCTGACACTTGCACTACTACTGGTAGTTATTATTTATTGAAATCCTCGCAAAACAGCAAAGTGATT
CCATTTGCGCCATTACGCAATAAAGATGGTTCAGGCTATACCGTGAGTATTTCAGCGCCTGTTGATTTTACGGATTTACA
AGATGAAACGGCGATTGCTGCGCGAATGAATCAAATCGTTGAAAAGGAAATCATGAAGGACATAACGATATATATGTGGC
TACATCGCCGTTTTAAAACACGTCCAGATGAAAAGACGCCTAGTTTATACGATTAA

Upstream 100 bases:

>100_bases
CCATTTTAAAATCTCTCTATTTGAATAACCAAAATTGTGGCGATTTTACCACAACTCAAATTTACGATAAACTACGCCCC
TAACTTACGTGGAAAGAACA

Downstream 100 bases:

>100_bases
AAGTGCGGTCGAATATCACCGCACTTTTTGATAGAATTTTCTCAAATTTAAAAAATTAACGAATAACCTCACCATGACAA
CCAATTATTCAGCTCAAGAA

Product: lipid A biosynthesis lauroyl acyltransferase

Products: NA

Alternate protein names: Heat shock protein B

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKNEKLPQFQPHFLAPKYWLFWLGVAIWRSILCLPYPILRHIGHGFGWLFSHLKVGERRAAIARRNLELCFPDMPENERE
VILQENLRSVGMAIIETGMAWFWSDSRIKKWSKVEGLHYLKENQKDGIVLVGVHFLTLELGARIIGLHHPGIGVYRPNDN
PLLDWLQTQGRLRSNKDMLDRKDLRGMIKALRHEETIWYAPDHDYGRKNAVFVPFFAVPDTCTTTGSYYLLKSSQNSKVI
PFAPLRNKDGSGYTVSISAPVDFTDLQDETAIAARMNQIVEKEIMKDITIYMWLHRRFKTRPDEKTPSLYD

Sequences:

>Translated_311_residues
MKNEKLPQFQPHFLAPKYWLFWLGVAIWRSILCLPYPILRHIGHGFGWLFSHLKVGERRAAIARRNLELCFPDMPENERE
VILQENLRSVGMAIIETGMAWFWSDSRIKKWSKVEGLHYLKENQKDGIVLVGVHFLTLELGARIIGLHHPGIGVYRPNDN
PLLDWLQTQGRLRSNKDMLDRKDLRGMIKALRHEETIWYAPDHDYGRKNAVFVPFFAVPDTCTTTGSYYLLKSSQNSKVI
PFAPLRNKDGSGYTVSISAPVDFTDLQDETAIAARMNQIVEKEIMKDITIYMWLHRRFKTRPDEKTPSLYD
>Mature_311_residues
MKNEKLPQFQPHFLAPKYWLFWLGVAIWRSILCLPYPILRHIGHGFGWLFSHLKVGERRAAIARRNLELCFPDMPENERE
VILQENLRSVGMAIIETGMAWFWSDSRIKKWSKVEGLHYLKENQKDGIVLVGVHFLTLELGARIIGLHHPGIGVYRPNDN
PLLDWLQTQGRLRSNKDMLDRKDLRGMIKALRHEETIWYAPDHDYGRKNAVFVPFFAVPDTCTTTGSYYLLKSSQNSKVI
PFAPLRNKDGSGYTVSISAPVDFTDLQDETAIAARMNQIVEKEIMKDITIYMWLHRRFKTRPDEKTPSLYD

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family

Homologues:

Organism=Escherichia coli, GI1787292, Length=307, Percent_Identity=55.3745928338762, Blast_Score=343, Evalue=9e-96,
Organism=Escherichia coli, GI87082094, Length=305, Percent_Identity=47.8688524590164, Blast_Score=295, Evalue=3e-81,
Organism=Escherichia coli, GI1788161, Length=315, Percent_Identity=28.8888888888889, Blast_Score=107, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HTRB_HAEIN (P45239)

Other databases:

- EMBL:   L42023
- EMBL:   U17642
- PIR:   D64127
- RefSeq:   NP_439676.2
- ProteinModelPortal:   P45239
- GeneID:   950389
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1527
- NMPDR:   fig|71421.1.peg.1454
- TIGR:   HI_1527
- HOGENOM:   HBG730280
- OMA:   TIWYAPD
- ProtClustDB:   PRK06860
- BioCyc:   HINF71421:HI_1527-MONOMER
- InterPro:   IPR004960
- InterPro:   IPR011920
- TIGRFAMs:   TIGR02207

Pfam domain/function: PF03279 Lip_A_acyltrans

EC number: 2.3.1.-

Molecular weight: Translated: 36152; Mature: 36152

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xdd78934)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNEKLPQFQPHFLAPKYWLFWLGVAIWRSILCLPYPILRHIGHGFGWLFSHLKVGERRA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHCHHHH
AIARRNLELCFPDMPENEREVILQENLRSVGMAIIETGMAWFWSDSRIKKWSKVEGLHYL
HHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHH
KENQKDGIVLVGVHFLTLELGARIIGLHHPGIGVYRPNDNPLLDWLQTQGRLRSNKDMLD
HCCCCCCEEEEHHHHHHHHHCCEEEEEECCCCEEECCCCCHHHHHHHHCCCCCCCHHHHH
RKDLRGMIKALRHEETIWYAPDHDYGRKNAVFVPFFAVPDTCTTTGSYYLLKSSQNSKVI
HHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCEEEEECCCCCEEE
PFAPLRNKDGSGYTVSISAPVDFTDLQDETAIAARMNQIVEKEIMKDITIYMWLHRRFKT
EECCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RPDEKTPSLYD
CCCCCCCCCCH
>Mature Secondary Structure
MKNEKLPQFQPHFLAPKYWLFWLGVAIWRSILCLPYPILRHIGHGFGWLFSHLKVGERRA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHCHHHH
AIARRNLELCFPDMPENEREVILQENLRSVGMAIIETGMAWFWSDSRIKKWSKVEGLHYL
HHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHH
KENQKDGIVLVGVHFLTLELGARIIGLHHPGIGVYRPNDNPLLDWLQTQGRLRSNKDMLD
HCCCCCCEEEEHHHHHHHHHCCEEEEEECCCCEEECCCCCHHHHHHHHCCCCCCCHHHHH
RKDLRGMIKALRHEETIWYAPDHDYGRKNAVFVPFFAVPDTCTTTGSYYLLKSSQNSKVI
HHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCEEEEECCCCCEEE
PFAPLRNKDGSGYTVSISAPVDFTDLQDETAIAARMNQIVEKEIMKDITIYMWLHRRFKT
EECCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RPDEKTPSLYD
CCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800; 7592970