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The map label for this gene is parE

Identifier: 16273428

GI number: 16273428

Start: 1598289

End: 1600187

Strand: Direct

Name: parE

Synonym: HI1528

Alternate gene names: 16273428

Gene position: 1598289-1600187 (Clockwise)

Preceding gene: 30995456

Following gene: 16273429

Centisome position: 87.33

GC content: 41.71

Gene sequence:

>1899_bases
ATGACAACCAATTATTCAGCTCAAGAAATTACCGTTCTTAAAGATCTCGAACCAGTGCAAATTCGCCCGGGAATGTACAC
TGACACTACTCGCCCTAATCATTTAGCACAAGAAGTTATTGATAACAGTGTGGATGAAGCCCTTGCCGGTTTTGCCACAA
AAATTGAAGTAATTTTACACCCTGATCAATCCATTGAAGTCACAGACAATGGTCGCGGTATGCCTGTGGATATTCATCCT
ACGGAAGGCGTTTCTGGCGTAGAAGTGATCCTGACTAAACTGCATGCTGGTGGTAAATTCTCCAATAAAAATTATGAATT
TGCGGGAGGTTTACACGGTGTGGGGATTTCTGTGGTAAATGCGCTTTCTGAACGCGTGGATATTCAAGTCAAACGCAATG
GCGAAATCTATAAAATTGCCTTTGAAAACGGCAGTAAAGTAGAAGAATTGGAAATTATCGGGACTTGTGGTAGACGCACA
ACTGGAACAATCGTTCACTTCAAACCAAATCCAAAATATTTTGACAGCGCAAAATTTTCCGTTAGCCGCCTACGCCATTT
ATTAAGAGCGAAAGCAGTCCTCTGTTCAGGGCTTGAAATCAAATTCATTGATAAAGTAAACAATACTCAAGACATTTGGT
TATATGAAGATGGGCTTTCCGATTACCTTATCGAAGCAGTAAATGGCTTTGAAACCCTTCCGAAAAAACCATTTGTTGGC
GAATTTAAAGGAGCAAATGAAGCGGTTAGCTGGGCATTACTTTGGTTGCCAGAAGGTGGCGAACTAATCGGCGAAAGCTA
CGTAAACTTAATTCCGACTATTCAAGGCGGAACGCATGTTAATGGCCTACGCCAAGGTTTATTAGATGCTATTCGTGAAT
TTTGCGAATTTAGAAATCTATTACCACGTGGGGTAAAACTTACCGCCGATGATATTTGGGATCGCTGTTCTTACATTCTT
TCCCTTAAAATGCAGGATGCGCAATTCGCAGGGCAAACAAAGGAACGCTTATCATCACGCCAAAGTGCGGTGTTTGTCAG
CGGTGTTTTAAAAGATGCGTTCAGCCTATGGCTAAATCAAAATGTACAAGATGCTGAAAAATTGGCAGAAATTGCTATTA
GTTCTGCACAACGCCGATTACGTGCAGCAAAAAAAGTCGTGCGAAAAAAACTCGTTAGTGGCCCTGCATTACCAGGAAAA
TTAGCTGATTGTGGCTCACAGGATTTAGAAAAAACGGAATTATTTTTAGTGGAGGGCGACTCTGCGGGCGGTTCAGCAAA
ACAAGCGCGTGATCGCGAATATCAAGCGATTTTGCCGTTACGTGGGAAAATCTTAAACACATGGGAAGTTTCCCCTGATC
AAGTATTAGGTTCAACCGAAATTCACGATATCGCCGTGGCTCTAGGGATCGATCCTGACAGCAATGATTTATCACAACTT
CGCTATGGTAAAGTCTGTATTCTCGCGGATGCTGACTCCGATGGTCTGCATATTGCCACCCTACTCTGTGCCCTGTTCTT
ACGTCATTTTCCGAAATTGGTACAAGACGGTCACGTTTACGTAGCAATGCCACCACTTTATCGCATTGACTTAAATAAAG
AAGTCTTCTACGCATTAGATGAAAATGAAAAAGAGGCGATTTTAGATCGGTTGAAAAACAAAAAAGGCAAACCGAATGTT
CAACGCTTTAAAGGTTTAGGTGAAATGAACCCATCGCAATTGCGTGAAACAACAATGGATCCTAATACGCGCCGTCTCGT
TCAATTAACTTATGATTTAGGGGAAGATCAAGGCTCAGATACATTAGAACTGATGGATATGTTATTAGCGAAAAAACGTT
CCGAAGACCGTAAAAATTGGTTACAAGCGAAAGGCGATCAGGTTGATTTATCTGTTTAG

Upstream 100 bases:

>100_bases
GAAAAGACGCCTAGTTTATACGATTAAAAGTGCGGTCGAATATCACCGCACTTTTTGATAGAATTTTCTCAAATTTAAAA
AATTAACGAATAACCTCACC

Downstream 100 bases:

>100_bases
ACTAAATTAATCTCAGCAAATCCGAATTTTAATAAATTAAGAATATATCAATTAACAGAAACAAAAATGACAAATATCAA
CTATGAAGGCATTGAGCAAA

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B

Number of amino acids: Translated: 632; Mature: 631

Protein sequence:

>632_residues
MTTNYSAQEITVLKDLEPVQIRPGMYTDTTRPNHLAQEVIDNSVDEALAGFATKIEVILHPDQSIEVTDNGRGMPVDIHP
TEGVSGVEVILTKLHAGGKFSNKNYEFAGGLHGVGISVVNALSERVDIQVKRNGEIYKIAFENGSKVEELEIIGTCGRRT
TGTIVHFKPNPKYFDSAKFSVSRLRHLLRAKAVLCSGLEIKFIDKVNNTQDIWLYEDGLSDYLIEAVNGFETLPKKPFVG
EFKGANEAVSWALLWLPEGGELIGESYVNLIPTIQGGTHVNGLRQGLLDAIREFCEFRNLLPRGVKLTADDIWDRCSYIL
SLKMQDAQFAGQTKERLSSRQSAVFVSGVLKDAFSLWLNQNVQDAEKLAEIAISSAQRRLRAAKKVVRKKLVSGPALPGK
LADCGSQDLEKTELFLVEGDSAGGSAKQARDREYQAILPLRGKILNTWEVSPDQVLGSTEIHDIAVALGIDPDSNDLSQL
RYGKVCILADADSDGLHIATLLCALFLRHFPKLVQDGHVYVAMPPLYRIDLNKEVFYALDENEKEAILDRLKNKKGKPNV
QRFKGLGEMNPSQLRETTMDPNTRRLVQLTYDLGEDQGSDTLELMDMLLAKKRSEDRKNWLQAKGDQVDLSV

Sequences:

>Translated_632_residues
MTTNYSAQEITVLKDLEPVQIRPGMYTDTTRPNHLAQEVIDNSVDEALAGFATKIEVILHPDQSIEVTDNGRGMPVDIHP
TEGVSGVEVILTKLHAGGKFSNKNYEFAGGLHGVGISVVNALSERVDIQVKRNGEIYKIAFENGSKVEELEIIGTCGRRT
TGTIVHFKPNPKYFDSAKFSVSRLRHLLRAKAVLCSGLEIKFIDKVNNTQDIWLYEDGLSDYLIEAVNGFETLPKKPFVG
EFKGANEAVSWALLWLPEGGELIGESYVNLIPTIQGGTHVNGLRQGLLDAIREFCEFRNLLPRGVKLTADDIWDRCSYIL
SLKMQDAQFAGQTKERLSSRQSAVFVSGVLKDAFSLWLNQNVQDAEKLAEIAISSAQRRLRAAKKVVRKKLVSGPALPGK
LADCGSQDLEKTELFLVEGDSAGGSAKQARDREYQAILPLRGKILNTWEVSPDQVLGSTEIHDIAVALGIDPDSNDLSQL
RYGKVCILADADSDGLHIATLLCALFLRHFPKLVQDGHVYVAMPPLYRIDLNKEVFYALDENEKEAILDRLKNKKGKPNV
QRFKGLGEMNPSQLRETTMDPNTRRLVQLTYDLGEDQGSDTLELMDMLLAKKRSEDRKNWLQAKGDQVDLSV
>Mature_631_residues
TTNYSAQEITVLKDLEPVQIRPGMYTDTTRPNHLAQEVIDNSVDEALAGFATKIEVILHPDQSIEVTDNGRGMPVDIHPT
EGVSGVEVILTKLHAGGKFSNKNYEFAGGLHGVGISVVNALSERVDIQVKRNGEIYKIAFENGSKVEELEIIGTCGRRTT
GTIVHFKPNPKYFDSAKFSVSRLRHLLRAKAVLCSGLEIKFIDKVNNTQDIWLYEDGLSDYLIEAVNGFETLPKKPFVGE
FKGANEAVSWALLWLPEGGELIGESYVNLIPTIQGGTHVNGLRQGLLDAIREFCEFRNLLPRGVKLTADDIWDRCSYILS
LKMQDAQFAGQTKERLSSRQSAVFVSGVLKDAFSLWLNQNVQDAEKLAEIAISSAQRRLRAAKKVVRKKLVSGPALPGKL
ADCGSQDLEKTELFLVEGDSAGGSAKQARDREYQAILPLRGKILNTWEVSPDQVLGSTEIHDIAVALGIDPDSNDLSQLR
YGKVCILADADSDGLHIATLLCALFLRHFPKLVQDGHVYVAMPPLYRIDLNKEVFYALDENEKEAILDRLKNKKGKPNVQ
RFKGLGEMNPSQLRETTMDPNTRRLVQLTYDLGEDQGSDTLELMDMLLAKKRSEDRKNWLQAKGDQVDLSV

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family

Homologues:

Organism=Homo sapiens, GI19913408, Length=627, Percent_Identity=25.9968102073365, Blast_Score=139, Evalue=8e-33,
Organism=Homo sapiens, GI19913406, Length=635, Percent_Identity=24.4094488188976, Blast_Score=137, Evalue=4e-32,
Organism=Escherichia coli, GI1789408, Length=632, Percent_Identity=76.4240506329114, Blast_Score=1031, Evalue=0.0,
Organism=Escherichia coli, GI48994957, Length=554, Percent_Identity=39.3501805054152, Blast_Score=361, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17535065, Length=629, Percent_Identity=25.9141494435612, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI212645845, Length=607, Percent_Identity=26.0296540362438, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI212645657, Length=230, Percent_Identity=28.2608695652174, Blast_Score=84, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324241, Length=653, Percent_Identity=25.114854517611, Blast_Score=144, Evalue=4e-35,
Organism=Drosophila melanogaster, GI17136538, Length=642, Percent_Identity=23.6760124610592, Blast_Score=132, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PARE_HAEIN (P43703)

Other databases:

- EMBL:   L42023
- PIR:   E64127
- RefSeq:   NP_439677.1
- ProteinModelPortal:   P43703
- GeneID:   950390
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1528
- NMPDR:   fig|71421.1.peg.1455
- TIGR:   HI_1528
- HOGENOM:   HBG304889
- OMA:   QARDKEF
- ProtClustDB:   PRK05559
- BioCyc:   HINF71421:HI_1528-MONOMER
- GO:   GO:0005694
- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737
- Gene3D:   G3DSA:3.30.565.10
- Gene3D:   G3DSA:3.30.230.10
- Gene3D:   G3DSA:3.40.50.670
- PRINTS:   PR00418
- SMART:   SM00387
- SMART:   SM00433
- TIGRFAMs:   TIGR01055

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; SSF55874 ATP_bd_ATPase; SSF54211 Ribosomal_S5_D2-typ_fold; SSF56719 Topo_IIA_cen

EC number: 5.99.1.-

Molecular weight: Translated: 70174; Mature: 70043

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNYSAQEITVLKDLEPVQIRPGMYTDTTRPNHLAQEVIDNSVDEALAGFATKIEVILH
CCCCCCCCEEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHEEEEEEEC
PDQSIEVTDNGRGMPVDIHPTEGVSGVEVILTKLHAGGKFSNKNYEFAGGLHGVGISVVN
CCCCEEECCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHH
ALSERVDIQVKRNGEIYKIAFENGSKVEELEIIGTCGRRTTGTIVHFKPNPKYFDSAKFS
HHHHHEEEEEECCCCEEEEEECCCCCEEEEEEEECCCCCCCCEEEEECCCCCCCCCCHHH
VSRLRHLLRAKAVLCSGLEIKFIDKVNNTQDIWLYEDGLSDYLIEAVNGFETLPKKPFVG
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCC
EFKGANEAVSWALLWLPEGGELIGESYVNLIPTIQGGTHVNGLRQGLLDAIREFCEFRNL
CCCCCCCEEEEEEEEECCCCHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
LPRGVKLTADDIWDRCSYILSLKMQDAQFAGQTKERLSSRQSAVFVSGVLKDAFSLWLNQ
CCCCCEEEHHHHHHHHHHHHHEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NVQDAEKLAEIAISSAQRRLRAAKKVVRKKLVSGPALPGKLADCGSQDLEKTELFLVEGD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCEEEEEEECC
SAGGSAKQARDREYQAILPLRGKILNTWEVSPDQVLGSTEIHDIAVALGIDPDSNDLSQL
CCCCCHHHHCCCCCEEECCCCCEEEEEECCCHHHHCCCCCCEEEEEEEECCCCCCHHHHH
RYGKVCILADADSDGLHIATLLCALFLRHFPKLVQDGHVYVAMPPLYRIDLNKEVFYALD
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEECCCEEEEEEC
ENEKEAILDRLKNKKGKPNVQRFKGLGEMNPSQLRETTMDPNTRRLVQLTYDLGEDQGSD
CCCHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHCCCCCCCEEEEEEEECCCCCCCC
TLELMDMLLAKKRSEDRKNWLQAKGDQVDLSV
HHHHHHHHHHHHCCHHHHHHHHCCCCEEECCC
>Mature Secondary Structure 
TTNYSAQEITVLKDLEPVQIRPGMYTDTTRPNHLAQEVIDNSVDEALAGFATKIEVILH
CCCCCCCEEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHEEEEEEEC
PDQSIEVTDNGRGMPVDIHPTEGVSGVEVILTKLHAGGKFSNKNYEFAGGLHGVGISVVN
CCCCEEECCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHH
ALSERVDIQVKRNGEIYKIAFENGSKVEELEIIGTCGRRTTGTIVHFKPNPKYFDSAKFS
HHHHHEEEEEECCCCEEEEEECCCCCEEEEEEEECCCCCCCCEEEEECCCCCCCCCCHHH
VSRLRHLLRAKAVLCSGLEIKFIDKVNNTQDIWLYEDGLSDYLIEAVNGFETLPKKPFVG
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCC
EFKGANEAVSWALLWLPEGGELIGESYVNLIPTIQGGTHVNGLRQGLLDAIREFCEFRNL
CCCCCCCEEEEEEEEECCCCHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
LPRGVKLTADDIWDRCSYILSLKMQDAQFAGQTKERLSSRQSAVFVSGVLKDAFSLWLNQ
CCCCCEEEHHHHHHHHHHHHHEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NVQDAEKLAEIAISSAQRRLRAAKKVVRKKLVSGPALPGKLADCGSQDLEKTELFLVEGD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCEEEEEEECC
SAGGSAKQARDREYQAILPLRGKILNTWEVSPDQVLGSTEIHDIAVALGIDPDSNDLSQL
CCCCCHHHHCCCCCEEECCCCCEEEEEECCCHHHHCCCCCCEEEEEEEECCCCCCHHHHH
RYGKVCILADADSDGLHIATLLCALFLRHFPKLVQDGHVYVAMPPLYRIDLNKEVFYALD
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEECCCEEEEEEC
ENEKEAILDRLKNKKGKPNVQRFKGLGEMNPSQLRETTMDPNTRRLVQLTYDLGEDQGSD
CCCHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHCCCCCCCEEEEEEEECCCCCCCC
TLELMDMLLAKKRSEDRKNWLQAKGDQVDLSV
HHHHHHHHHHHHCCHHHHHHHHCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800