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The map label for this gene is murI [H]

Identifier: 16273625

GI number: 16273625

Start: 1823723

End: 1824532

Strand: Reverse

Name: murI [H]

Synonym: HI1739.2

Alternate gene names: 16273625

Gene position: 1824532-1823723 (Counterclockwise)

Preceding gene: 16273626

Following gene: 16273624

Centisome position: 99.69

GC content: 36.54

Gene sequence:

>810_bases
ATGGATAAAAAAGAAAAACGCCCAACAGTGCTCTTTTTTGATTCTGGAGTGGGTGGGTTTAGCGTATATCGTGAAGCCAA
AAAACTATTACCCAATTGGCATTATCTCTATTGCTTTGATAATGCGGGATTCCCTTATTCAGAACGCAAAGAAGAAAGTA
TTATTCACCGAACTTTAGCGGCGTGTCAGCTTATTAATCAACGTTATCCATTAGATGCAATGGTGATAGCTTGCAATACA
GCGAGTACTGTTGTGCTTCCGCCTTTGCGAGCTGCTTTTGATATTCCTATCATTGGGACTGTACCCGCTATTAAACCTGC
ATCAGAAATAACAAAGACAAAACATATTGGTTTATTAGCTACAAAGGGGACTGTAAAACGTCATTATATCGATGAGTTGA
TTGATAAATTTGCGCAAGATTGTATTGTTGAGAGACTGGGAACGACAAAATTAGTCGAAATTGCGGAGCAAAAAATTCGT
GGTCATTCCGTTGATCTCATTAGTTTAAAAGATGAGTTATCTTCGTGGGCAGGCATGGCAGATTTGGATACATTAGTTTT
AGGTTGTACTCACTTTCCTCTCATCAAAGATGAAATTCAGTTATGCTTGCCACAAGTTAAATATTTTATGGAACCGAGCG
CAGCAATTGCTAAACGGATCAAATATTTACTTGATGATAAAAATCTACAAGCGCAAAATGAAAAATATAATCAAATGTTT
TGCACCGCACATTTTCCCGAAGAATCTCAATTTAAAAAAGCTTTACATCTATGGGGATTTGAATCTTTGGAAGTAATCAA
AATAGATTAA

Upstream 100 bases:

>100_bases
AATACCCTGATGTGGCAGAAAGCCTAATCCGTCGCTGGCTCAATAATAAAGAAATTTACTCGAACGCTTAAAAGTGCGGT
CAATTTTCAAAGAGTTTTAA

Downstream 100 bases:

>100_bases
AAGTTAATAGATTAATTGTGTGATATTGATAAATTCACAATTTTTCCTAATTTTATAGGTGATTTTTCTACTTATATCAT
TTCTATATGTCACTTTTTTA

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MDKKEKRPTVLFFDSGVGGFSVYREAKKLLPNWHYLYCFDNAGFPYSERKEESIIHRTLAACQLINQRYPLDAMVIACNT
ASTVVLPPLRAAFDIPIIGTVPAIKPASEITKTKHIGLLATKGTVKRHYIDELIDKFAQDCIVERLGTTKLVEIAEQKIR
GHSVDLISLKDELSSWAGMADLDTLVLGCTHFPLIKDEIQLCLPQVKYFMEPSAAIAKRIKYLLDDKNLQAQNEKYNQMF
CTAHFPEESQFKKALHLWGFESLEVIKID

Sequences:

>Translated_269_residues
MDKKEKRPTVLFFDSGVGGFSVYREAKKLLPNWHYLYCFDNAGFPYSERKEESIIHRTLAACQLINQRYPLDAMVIACNT
ASTVVLPPLRAAFDIPIIGTVPAIKPASEITKTKHIGLLATKGTVKRHYIDELIDKFAQDCIVERLGTTKLVEIAEQKIR
GHSVDLISLKDELSSWAGMADLDTLVLGCTHFPLIKDEIQLCLPQVKYFMEPSAAIAKRIKYLLDDKNLQAQNEKYNQMF
CTAHFPEESQFKKALHLWGFESLEVIKID
>Mature_269_residues
MDKKEKRPTVLFFDSGVGGFSVYREAKKLLPNWHYLYCFDNAGFPYSERKEESIIHRTLAACQLINQRYPLDAMVIACNT
ASTVVLPPLRAAFDIPIIGTVPAIKPASEITKTKHIGLLATKGTVKRHYIDELIDKFAQDCIVERLGTTKLVEIAEQKIR
GHSVDLISLKDELSSWAGMADLDTLVLGCTHFPLIKDEIQLCLPQVKYFMEPSAAIAKRIKYLLDDKNLQAQNEKYNQMF
CTAHFPEESQFKKALHLWGFESLEVIKID

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis [H]

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family [H]

Homologues:

Organism=Escherichia coli, GI87082355, Length=266, Percent_Identity=48.8721804511278, Blast_Score=259, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391 [H]

Pfam domain/function: PF01177 Asp_Glu_race [H]

EC number: =5.1.1.3 [H]

Molecular weight: Translated: 30535; Mature: 30535

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00923 ASP_GLU_RACEMASE_1 ; PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKKEKRPTVLFFDSGVGGFSVYREAKKLLPNWHYLYCFDNAGFPYSERKEESIIHRTLA
CCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHH
ACQLINQRYPLDAMVIACNTASTVVLPPLRAAFDIPIIGTVPAIKPASEITKTKHIGLLA
HHHHHHCCCCCCEEEEEECCCCEEEECCHHHHCCCCEECCCCCCCCHHHHHHHHCCEEEE
TKGTVKRHYIDELIDKFAQDCIVERLGTTKLVEIAEQKIRGHSVDLISLKDELSSWAGMA
ECCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCH
DLDTLVLGCTHFPLIKDEIQLCLPQVKYFMEPSAAIAKRIKYLLDDKNLQAQNEKYNQMF
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCEEE
CTAHFPEESQFKKALHLWGFESLEVIKID
EEEECCCHHHHHHHHHHCCCCCEEEEEEC
>Mature Secondary Structure
MDKKEKRPTVLFFDSGVGGFSVYREAKKLLPNWHYLYCFDNAGFPYSERKEESIIHRTLA
CCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHH
ACQLINQRYPLDAMVIACNTASTVVLPPLRAAFDIPIIGTVPAIKPASEITKTKHIGLLA
HHHHHHCCCCCCEEEEEECCCCEEEECCHHHHCCCCEECCCCCCCCHHHHHHHHCCEEEE
TKGTVKRHYIDELIDKFAQDCIVERLGTTKLVEIAEQKIRGHSVDLISLKDELSSWAGMA
ECCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCH
DLDTLVLGCTHFPLIKDEIQLCLPQVKYFMEPSAAIAKRIKYLLDDKNLQAQNEKYNQMF
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCEEE
CTAHFPEESQFKKALHLWGFESLEVIKID
EEEECCCHHHHHHHHHHCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA