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The map label for this gene is metR

Identifier: 16273623

GI number: 16273623

Start: 1815309

End: 1816238

Strand: Reverse

Name: metR

Synonym: HI1739

Alternate gene names: 16273623

Gene position: 1816238-1815309 (Counterclockwise)

Preceding gene: 16273624

Following gene: 16273622

Centisome position: 99.24

GC content: 38.82

Gene sequence:

>930_bases
ATGAAACCAACCTTTCTAGAATTTAGACATCTTAAAACTTTATTGGCTTTAAAAGAGACAGGCAGCGTTTCTCTAGCTGC
AAAGCGCGTATATCTAACCCAATCAGCACTTTCTCACCAAATTAAATTAATAGAAGAACAATTTGGCTTGCCTTTATTTG
AGCGTAAAAGTAATCCACTTCGTTTTACTTCAGCGGGAGAGCGTTTAATTCGTCTTGCGAATGAAGTAATGCCGAAAGTG
ATTGATGCTGAACGTGATCTTGCACGGGTAAAACACGGCGATGCAGGACAGTTACGTATAGCGGTGGAATGCCATACTTG
TTTTGATTGGTTAATGCCAGCGATGGATGAATTCCGTCAGCATTGGGGGCTAGTAGAACTGGATATTGTATCTGGCTTTC
ATACTGATCCCGTTGGTTTATTACTTTCCCATCGAGCAGATTGGGCGATAGTATCAGAAATAGAGCATAATGATGATGTC
ATTTTTAAACCGCTCTTTTCTTATGAAATGGTTGGGATTTGCTCAAAAAATCATTCTTTAGCAGAAAAAGATATTTGGGA
AGCGGAAGATTTTATTGATGAAACTTGGGTAACTTACCCCGTACCTGACGATATGTTAGATCTATGGCGTAAAGTGCTTA
AATCAAAAGGCATTAATCCTACTCGCCGTACAACAGAACTTACTATTGCAATGATTCAACTTGTTGCGAGTCGTCGTGGT
ATTGCGACTATCCCTTATTGGGCTGCGCTGCCTTATTTGGAAAAGGGTTATGTCGTGGCAAGAAAAGTCACAAAAGAAGG
TTTATATAGCAATCTATATGCGGCGATTCGCAAAGAAGATGAATCCCTTTCTTATTTAGAAGATTTTTATCAAACAGTCA
AATCTCAAAGTTTCTCAACTTTACCCGGACTTTCTGTTTTAAATTTATAG

Upstream 100 bases:

>100_bases
AAAATAATTATCTAAATAATTAATTTTATTAATTAGCATATTTCTAATTTTAATGTATTATACTGGTTCCCTAATTCTTA
ATTTTATATGAATATTATTA

Downstream 100 bases:

>100_bases
ACATAATTAATGAATTTATCTCAACAAAATCAACACTCTAACCCAATTACTGAAGCGGCTAAGGCTACTTTTCCCTACAG
CGTACCAATGATTGCAGGAT

Product: transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKPTFLEFRHLKTLLALKETGSVSLAAKRVYLTQSALSHQIKLIEEQFGLPLFERKSNPLRFTSAGERLIRLANEVMPKV
IDAERDLARVKHGDAGQLRIAVECHTCFDWLMPAMDEFRQHWGLVELDIVSGFHTDPVGLLLSHRADWAIVSEIEHNDDV
IFKPLFSYEMVGICSKNHSLAEKDIWEAEDFIDETWVTYPVPDDMLDLWRKVLKSKGINPTRRTTELTIAMIQLVASRRG
IATIPYWAALPYLEKGYVVARKVTKEGLYSNLYAAIRKEDESLSYLEDFYQTVKSQSFSTLPGLSVLNL

Sequences:

>Translated_309_residues
MKPTFLEFRHLKTLLALKETGSVSLAAKRVYLTQSALSHQIKLIEEQFGLPLFERKSNPLRFTSAGERLIRLANEVMPKV
IDAERDLARVKHGDAGQLRIAVECHTCFDWLMPAMDEFRQHWGLVELDIVSGFHTDPVGLLLSHRADWAIVSEIEHNDDV
IFKPLFSYEMVGICSKNHSLAEKDIWEAEDFIDETWVTYPVPDDMLDLWRKVLKSKGINPTRRTTELTIAMIQLVASRRG
IATIPYWAALPYLEKGYVVARKVTKEGLYSNLYAAIRKEDESLSYLEDFYQTVKSQSFSTLPGLSVLNL
>Mature_309_residues
MKPTFLEFRHLKTLLALKETGSVSLAAKRVYLTQSALSHQIKLIEEQFGLPLFERKSNPLRFTSAGERLIRLANEVMPKV
IDAERDLARVKHGDAGQLRIAVECHTCFDWLMPAMDEFRQHWGLVELDIVSGFHTDPVGLLLSHRADWAIVSEIEHNDDV
IFKPLFSYEMVGICSKNHSLAEKDIWEAEDFIDETWVTYPVPDDMLDLWRKVLKSKGINPTRRTTELTIAMIQLVASRRG
IATIPYWAALPYLEKGYVVARKVTKEGLYSNLYAAIRKEDESLSYLEDFYQTVKSQSFSTLPGLSVLNL

Specific function: Control of the last step in methionine biosynthesis; metR is a positive activator of the metA, metE and metH genes

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1790262, Length=298, Percent_Identity=39.261744966443, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI145693105, Length=186, Percent_Identity=26.3440860215054, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI157672245, Length=269, Percent_Identity=24.5353159851301, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787530, Length=183, Percent_Identity=30.0546448087432, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1790208, Length=250, Percent_Identity=27.6, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786401, Length=158, Percent_Identity=27.2151898734177, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87082024, Length=288, Percent_Identity=21.875, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787879, Length=247, Percent_Identity=23.0769230769231, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI87082132, Length=261, Percent_Identity=24.5210727969349, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): METR_HAEIN (P45349)

Other databases:

- EMBL:   L42023
- PIR:   D64139
- RefSeq:   NP_439881.1
- ProteinModelPortal:   P45349
- GeneID:   950886
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1739
- NMPDR:   fig|71421.1.peg.1651
- TIGR:   HI_1739
- HOGENOM:   HBG685425
- OMA:   EQRQPVI
- ProtClustDB:   CLSK870088
- BioCyc:   HINF71421:HI_1739-MONOMER
- GO:   GO:0005737
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00039

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 35345; Mature: 35345

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS50931 HTH_LYSR; PS00300 SRP54

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPTFLEFRHLKTLLALKETGSVSLAAKRVYLTQSALSHQIKLIEEQFGLPLFERKSNPL
CCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCE
RFTSAGERLIRLANEVMPKVIDAERDLARVKHGDAGQLRIAVECHTCFDWLMPAMDEFRQ
EECHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHH
HWGLVELDIVSGFHTDPVGLLLSHRADWAIVSEIEHNDDVIFKPLFSYEMVGICSKNHSL
HCCEEEEEEECCCCCCCHHHHHHCCCCEEEEECCCCCCCEEEEHHHHHHHHHHCCCCCCH
AEKDIWEAEDFIDETWVTYPVPDDMLDLWRKVLKSKGINPTRRTTELTIAMIQLVASRRG
HHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
IATIPYWAALPYLEKGYVVARKVTKEGLYSNLYAAIRKEDESLSYLEDFYQTVKSQSFST
CEECCHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCC
LPGLSVLNL
CCCCHHCCC
>Mature Secondary Structure
MKPTFLEFRHLKTLLALKETGSVSLAAKRVYLTQSALSHQIKLIEEQFGLPLFERKSNPL
CCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCE
RFTSAGERLIRLANEVMPKVIDAERDLARVKHGDAGQLRIAVECHTCFDWLMPAMDEFRQ
EECHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHH
HWGLVELDIVSGFHTDPVGLLLSHRADWAIVSEIEHNDDVIFKPLFSYEMVGICSKNHSL
HCCEEEEEEECCCCCCCHHHHHHCCCCEEEEECCCCCCCEEEEHHHHHHHHHHCCCCCCH
AEKDIWEAEDFIDETWVTYPVPDDMLDLWRKVLKSKGINPTRRTTELTIAMIQLVASRRG
HHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
IATIPYWAALPYLEKGYVVARKVTKEGLYSNLYAAIRKEDESLSYLEDFYQTVKSQSFST
CEECCHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCC
LPGLSVLNL
CCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800