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The map label for this gene is ilvI

Identifier: 16273479

GI number: 16273479

Start: 1651814

End: 1653535

Strand: Reverse

Name: ilvI

Synonym: HI1585

Alternate gene names: 16273479

Gene position: 1653535-1651814 (Counterclockwise)

Preceding gene: 16273480

Following gene: 16273478

Centisome position: 90.35

GC content: 39.2

Gene sequence:

>1722_bases
ATGAAGAAGTTATCTGGCGCAGAGATGGTGGTTCAATCTTTACGTGATGAAGGCGTAGAGTATGTATTTGGTTATCCCGG
AGGCGCGGTATTAGATATTTATGATGCAATTCATACGCTGGGTGGTATTGAACATATTTTAGTTCGCCACGAACAAGCCG
CGGTGCATATGGCGGATGGTTACGCACGTTCAACGGGTAAAGTTGGATGTGTGCTAGTGACTTCGGGGCCTGGTGCAACA
AATGCTATCACTGGCATTTTAACGGCTTATACCGATTCTGTGCCTATGGTTATCATTTCGGGTCAGGTTATGAGCAATTT
AATTGGCAGTGATGCTTTCCAAGAATGTGATATGCTGGGTATTTCTCGTCCCGTTGTGAAGCATAGCTTTATTGTCAAAA
AGGCGGAAGATATTCCATCTACTTTGAAAAAAGCCTTTTATATTGCATCTACGGGTCGTCCAGGTCCTGTTGTTGTGGAT
ATTCCAAAAGACACTGTAAATCCTAATTTTAAATATCCTTATGAATACCCTGAATATGTCGAGTTACGTTCTTATAATCC
AACAGTAAATGGACACAAAGGTCAAATTAAAAAAGCGTTAAAAGCACTTTTAGTGGCGAAGAAACCAATACTTTTTGTCG
GTGGTGGTGCAATCACTGCTGAATGTAGTGAGCAGTTAATTCAGTTTGCACAACGTTTAAATTTACCCGTGACTTCCTCA
TTAATGGGATTGGGTGCTTATCCAAGTACGGATAAACAATTCTTAGGTATGTTGGGTATGCATGGTACTTTAGAAGCGAA
TACGGCAATGCACGAAAGTGATCTTATTTTGGGGATTGGCGTTCGTTTTGATGATCGTACGACAAATAATTTAGAAAAAT
ATTGCCCAAATGCAAAAGTGATTCATATTGATATTGATCCAACCTCAATTTCTAAAAATGTGCCAGTAGCGATTCCAATT
GTCGGAAATGCGAAAAATGTATTGGAAGAATTTTTGGGTTTATTGAATGAAGAGGGATTAAAATCTCAAACAGATCTTGA
AAGTTGGTGGCAAGAAATCAACCAATGGAAAGCAAAAAAATGTTTGGAATTTGACCGCACTTCTGGCGTGATTAAACCGC
AACAAGTCGTGGAAGCGGTATATCGCCTCACGAAAGGACAAGCTTATGTGGCTTCTGATGTAGGTCAGCACCAAATGTTT
GCTGCGTTACATTATCCATTCGATGAACCGCGTCATTGGATTAATTCTGGTGGTGCAGGCACAATGGGTTTTGGTTTTCC
TGCGGCACTTGGTGTTAAATTAGCTCATCCTGAAGGAACTGTTGTTTGCGTTACTGGCGATGGTAGTATTCAAATGAATA
TTCAAGAGCTTTCTACCGCAACACAATATGGCATTCCTGTTGTCATTATTTGTTTGAATAATCATTTCTTGGGAATGGTA
AAACAATGGCAAGATTTGATTTATTCTGGTCGCCATTCGCAAACCTATATGAATTCCTTGCCTGATTTTGCGAAATTGGC
AGAATCTTACGGTCACGTAGGAATAAAAATTGCTACCCCCGATGAACTTGAAAGCAAATTGCAAGAAGCATTCAGTATTA
AAAATAAATTAGTCTTTGTAGATATTAATGTAGATGAATCTGAACACGTTTATCCAATGCAAATTCGTGGTGGTGCAATG
AATGAAATGATTTTAAGTAAACCTCAAGAGGAGACAAACTAA

Upstream 100 bases:

>100_bases
GTTTTAGTGGCTAGACAAATTTTTTTTACTTTGTATTATGTGAAACATTGACATAATCTGTTCGTTTTTTTAAAAGTGCG
GTGAATTTAAGGAGTGAAAA

Downstream 100 bases:

>100_bases
TGCGTAGAATTTTATCTGTTTTACTCGAAAATGAATCGGGAGCATTGTCGCGTGTTGTTGGTTTATTCTCTCAACGTGCC
TTTAATATTGAAAGTTTGAC

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MKKLSGAEMVVQSLRDEGVEYVFGYPGGAVLDIYDAIHTLGGIEHILVRHEQAAVHMADGYARSTGKVGCVLVTSGPGAT
NAITGILTAYTDSVPMVIISGQVMSNLIGSDAFQECDMLGISRPVVKHSFIVKKAEDIPSTLKKAFYIASTGRPGPVVVD
IPKDTVNPNFKYPYEYPEYVELRSYNPTVNGHKGQIKKALKALLVAKKPILFVGGGAITAECSEQLIQFAQRLNLPVTSS
LMGLGAYPSTDKQFLGMLGMHGTLEANTAMHESDLILGIGVRFDDRTTNNLEKYCPNAKVIHIDIDPTSISKNVPVAIPI
VGNAKNVLEEFLGLLNEEGLKSQTDLESWWQEINQWKAKKCLEFDRTSGVIKPQQVVEAVYRLTKGQAYVASDVGQHQMF
AALHYPFDEPRHWINSGGAGTMGFGFPAALGVKLAHPEGTVVCVTGDGSIQMNIQELSTATQYGIPVVIICLNNHFLGMV
KQWQDLIYSGRHSQTYMNSLPDFAKLAESYGHVGIKIATPDELESKLQEAFSIKNKLVFVDINVDESEHVYPMQIRGGAM
NEMILSKPQEETN

Sequences:

>Translated_573_residues
MKKLSGAEMVVQSLRDEGVEYVFGYPGGAVLDIYDAIHTLGGIEHILVRHEQAAVHMADGYARSTGKVGCVLVTSGPGAT
NAITGILTAYTDSVPMVIISGQVMSNLIGSDAFQECDMLGISRPVVKHSFIVKKAEDIPSTLKKAFYIASTGRPGPVVVD
IPKDTVNPNFKYPYEYPEYVELRSYNPTVNGHKGQIKKALKALLVAKKPILFVGGGAITAECSEQLIQFAQRLNLPVTSS
LMGLGAYPSTDKQFLGMLGMHGTLEANTAMHESDLILGIGVRFDDRTTNNLEKYCPNAKVIHIDIDPTSISKNVPVAIPI
VGNAKNVLEEFLGLLNEEGLKSQTDLESWWQEINQWKAKKCLEFDRTSGVIKPQQVVEAVYRLTKGQAYVASDVGQHQMF
AALHYPFDEPRHWINSGGAGTMGFGFPAALGVKLAHPEGTVVCVTGDGSIQMNIQELSTATQYGIPVVIICLNNHFLGMV
KQWQDLIYSGRHSQTYMNSLPDFAKLAESYGHVGIKIATPDELESKLQEAFSIKNKLVFVDINVDESEHVYPMQIRGGAM
NEMILSKPQEETN
>Mature_573_residues
MKKLSGAEMVVQSLRDEGVEYVFGYPGGAVLDIYDAIHTLGGIEHILVRHEQAAVHMADGYARSTGKVGCVLVTSGPGAT
NAITGILTAYTDSVPMVIISGQVMSNLIGSDAFQECDMLGISRPVVKHSFIVKKAEDIPSTLKKAFYIASTGRPGPVVVD
IPKDTVNPNFKYPYEYPEYVELRSYNPTVNGHKGQIKKALKALLVAKKPILFVGGGAITAECSEQLIQFAQRLNLPVTSS
LMGLGAYPSTDKQFLGMLGMHGTLEANTAMHESDLILGIGVRFDDRTTNNLEKYCPNAKVIHIDIDPTSISKNVPVAIPI
VGNAKNVLEEFLGLLNEEGLKSQTDLESWWQEINQWKAKKCLEFDRTSGVIKPQQVVEAVYRLTKGQAYVASDVGQHQMF
AALHYPFDEPRHWINSGGAGTMGFGFPAALGVKLAHPEGTVVCVTGDGSIQMNIQELSTATQYGIPVVIICLNNHFLGMV
KQWQDLIYSGRHSQTYMNSLPDFAKLAESYGHVGIKIATPDELESKLQEAFSIKNKLVFVDINVDESEHVYPMQIRGGAM
NEMILSKPQEETN

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family

Homologues:

Organism=Homo sapiens, GI93004078, Length=566, Percent_Identity=26.5017667844523, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI21361361, Length=509, Percent_Identity=26.7190569744597, Blast_Score=157, Evalue=4e-38,
Organism=Escherichia coli, GI87081685, Length=573, Percent_Identity=70.6806282722513, Blast_Score=867, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=564, Percent_Identity=38.2978723404255, Blast_Score=405, Evalue=1e-114,
Organism=Escherichia coli, GI1786717, Length=545, Percent_Identity=33.7614678899083, Blast_Score=320, Evalue=2e-88,
Organism=Escherichia coli, GI1787096, Length=562, Percent_Identity=27.5800711743772, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1788716, Length=585, Percent_Identity=24.1025641025641, Blast_Score=162, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17531299, Length=560, Percent_Identity=27.8571428571429, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17531301, Length=560, Percent_Identity=27.8571428571429, Blast_Score=173, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17542570, Length=583, Percent_Identity=25.0428816466552, Blast_Score=142, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6323755, Length=588, Percent_Identity=40.3061224489796, Blast_Score=434, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6320816, Length=495, Percent_Identity=26.0606060606061, Blast_Score=120, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6323073, Length=564, Percent_Identity=21.6312056737589, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19922626, Length=578, Percent_Identity=24.7404844290657, Blast_Score=176, Evalue=4e-44,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): ILVI_HAEIN (P45261)

Other databases:

- EMBL:   L42023
- PIR:   C64131
- RefSeq:   NP_439730.1
- ProteinModelPortal:   P45261
- SMR:   P45261
- GeneID:   950449
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1585
- NMPDR:   fig|71421.1.peg.1505
- TIGR:   HI_1585
- HOGENOM:   HBG323037
- OMA:   RLVFIDI
- ProtClustDB:   PRK06882
- BioCyc:   HINF71421:HI_1585-MONOMER
- BRENDA:   2.2.1.6
- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00118

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N

EC number: =2.2.1.6

Molecular weight: Translated: 62621; Mature: 62621

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00187 TPP_ENZYMES

Important sites: BINDING 51-51 BINDING 153-153

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLSGAEMVVQSLRDEGVEYVFGYPGGAVLDIYDAIHTLGGIEHILVRHEQAAVHMADG
CCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHEEEECCC
YARSTGKVGCVLVTSGPGATNAITGILTAYTDSVPMVIISGQVMSNLIGSDAFQECDMLG
CCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHHHCCHHHHHHHHHC
ISRPVVKHSFIVKKAEDIPSTLKKAFYIASTGRPGPVVVDIPKDTVNPNFKYPYEYPEYV
CCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCEE
ELRSYNPTVNGHKGQIKKALKALLVAKKPILFVGGGAITAECSEQLIQFAQRLNLPVTSS
EEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCEEEHHHHHHHHHHHHHCCCCHHHH
LMGLGAYPSTDKQFLGMLGMHGTLEANTAMHESDLILGIGVRFDDRTTNNLEKYCPNAKV
HHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHCCCCEE
IHIDIDPTSISKNVPVAIPIVGNAKNVLEEFLGLLNEEGLKSQTDLESWWQEINQWKAKK
EEEECCCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
CLEFDRTSGVIKPQQVVEAVYRLTKGQAYVASDVGQHQMFAALHYPFDEPRHWINSGGAG
HHHHHCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHEEECCCCCHHHHHCCCCCC
TMGFGFPAALGVKLAHPEGTVVCVTGDGSIQMNIQELSTATQYGIPVVIICLNNHFLGMV
CCCCCCHHHHCEEEECCCCEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEECCCHHHHH
KQWQDLIYSGRHSQTYMNSLPDFAKLAESYGHVGIKIATPDELESKLQEAFSIKNKLVFV
HHHHHHHHCCCCCHHHHHCCCHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCCEEEEE
DINVDESEHVYPMQIRGGAMNEMILSKPQEETN
EEECCCCCCEEEEEECCCCHHHHHHCCCCCCCC
>Mature Secondary Structure
MKKLSGAEMVVQSLRDEGVEYVFGYPGGAVLDIYDAIHTLGGIEHILVRHEQAAVHMADG
CCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHEEEECCC
YARSTGKVGCVLVTSGPGATNAITGILTAYTDSVPMVIISGQVMSNLIGSDAFQECDMLG
CCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHHHCCHHHHHHHHHC
ISRPVVKHSFIVKKAEDIPSTLKKAFYIASTGRPGPVVVDIPKDTVNPNFKYPYEYPEYV
CCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCEE
ELRSYNPTVNGHKGQIKKALKALLVAKKPILFVGGGAITAECSEQLIQFAQRLNLPVTSS
EEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCEEEHHHHHHHHHHHHHCCCCHHHH
LMGLGAYPSTDKQFLGMLGMHGTLEANTAMHESDLILGIGVRFDDRTTNNLEKYCPNAKV
HHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHCCCCEE
IHIDIDPTSISKNVPVAIPIVGNAKNVLEEFLGLLNEEGLKSQTDLESWWQEINQWKAKK
EEEECCCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
CLEFDRTSGVIKPQQVVEAVYRLTKGQAYVASDVGQHQMFAALHYPFDEPRHWINSGGAG
HHHHHCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHEEECCCCCHHHHHCCCCCC
TMGFGFPAALGVKLAHPEGTVVCVTGDGSIQMNIQELSTATQYGIPVVIICLNNHFLGMV
CCCCCCHHHHCEEEECCCCEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEECCCHHHHH
KQWQDLIYSGRHSQTYMNSLPDFAKLAESYGHVGIKIATPDELESKLQEAFSIKNKLVFV
HHHHHHHHCCCCCHHHHHCCCHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCCEEEEE
DINVDESEHVYPMQIRGGAMNEMILSKPQEETN
EEECCCCCCEEEEEECCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800