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The map label for this gene is dnaB [H]

Identifier: 16273469

GI number: 16273469

Start: 1641702

End: 1643216

Strand: Direct

Name: dnaB [H]

Synonym: HI1574

Alternate gene names: 16273469

Gene position: 1641702-1643216 (Clockwise)

Preceding gene: 16273468

Following gene: 16273470

Centisome position: 89.7

GC content: 39.93

Gene sequence:

>1515_bases
GTGGATTTTTCCACCGTTTTTTATTTCCTTTTTTCACCGCACTTTTCACTTAAATCCAATAGATATTTGCGGTATCATAG
ACGACATTCTAGTATCGAAATAAGTCCTATGGCATCACAACCTCAAATCAAATCTTCAGACAAAAAAACAGCACAAGTTA
GCATTCCTCCGCACTCAACTGAGGCTGAACAAGCCGTGTTGGGTGGCATCATGCTGAGCAATCAACATTGGGATGGCATT
GCTGAACGTGTGATTGCTGACGATTTTTATACTTTTCAGCATCGTCTAATTTTTACAGAAATGGAACATCTAATGCGTAA
TCAATCGCCTATTGATTTAATTACGCTAGATCAAGCCTTAAGAAGCCGTGGTGTAAGCGATGAAGTAGGTGGATTTGCCT
ATCTAGCAGAGCTTTCCAATAATACTCCGAACGCCATTAATATTTTGGCTTATGCAGATATCGTGCGCGAGAAAGCCATA
TTACGAGAACTTATTTCGGTAGGGAATCGCATTGCTGAAAATAGCTATTCTCCTAAAGGGCAAGACATTAAGTTAATTCT
TGATGAGGCTGAGCGTGAAGTATTTGCGATTGCAGAAAAACGTACGACTTCTAGCGAAGGCCCACAAAATGTGATCAATG
TGCTAGAAAGTACGATTGAAAAAATCGATATTTTAAGCAAACTTGAAAATCATTCAGGTGTAACAGGTGTTACGACGGGC
TTTACCGATCTTGATAAAAAAACGGCTGGTTTACAACCTTCTGACTTAATTATCGTTGCGGCACGTCCGTCAATGGGTAA
AACCACTTTCGCCATGAACCTTTGCGAAAATGCCGCAATGGCAAGTGAAAAACCCGTTTTAGTATTTAGTTTAGAAATGC
CAGCAGAACAAATTATGATGCGTATGATCGCTTCCCTTGCTCGCGTTGATCAAACTAAAATCCGAACAGGTCAAAATTTA
GATGAAATCGAGTGGAACAAAATTGCCAGCGTAGTAGGAATGTTCAAGCAAAAAAATAATCTTTTTATCGATGATTCTTC
AGGTCTAACACCTACCGATGTTCGTTCGCGCGCACGCCGAGTTTATCGTGAAAATGGTGGATTAAGTATGATTATGGTGG
ATTATTTGCAATTAATGCGCGCACCAGCATTTTCAGATAACCGAACACTAGAAATCGCAGAAATTTCTCGCTCCCTCAAA
GCACTCGCCAAAGAATTACAAGTGCCAGTAGTCGCCCTTTCTCAGTTAAATCGTACTTTAGAACAACGTGGAGACAAACG
CCCTGTAAACTCAGATTTACGTGAATCTGGCTCTATTGAACAAGATGCAGACTTGATTATGTTTATTTATCGTGATGAAG
TCTATAACGATAACTCGGAAGATAAAGGTGTTGCAGAAATTATTATCGGTAAACAGCGTAACGGCCCAATTGGTCGAGTG
CGGTTAAAATTTAATGGACAATTTTCACGCTTCGACAATCTCGCCGAACAACGTGAATATCGAGATGATTATTAA

Upstream 100 bases:

>100_bases
GCTGGTAACCCAAGGTGGTCAAGGTGCGACACAAACTAACGTATGTCGTACATTAATTGTTGAATAATCAACAATCTAAA
TATGTTAAAATAAAAGAGCG

Downstream 100 bases:

>100_bases
GGAAAAATAATGAACGTAAAACCGGCGACAGCGAAAATTAGTTCGCACGCCTTAAAACAGAATTTAGAAATAATTAAACA
AAAAGCACCAAATAGCAAAA

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MDFSTVFYFLFSPHFSLKSNRYLRYHRRHSSIEISPMASQPQIKSSDKKTAQVSIPPHSTEAEQAVLGGIMLSNQHWDGI
AERVIADDFYTFQHRLIFTEMEHLMRNQSPIDLITLDQALRSRGVSDEVGGFAYLAELSNNTPNAINILAYADIVREKAI
LRELISVGNRIAENSYSPKGQDIKLILDEAEREVFAIAEKRTTSSEGPQNVINVLESTIEKIDILSKLENHSGVTGVTTG
FTDLDKKTAGLQPSDLIIVAARPSMGKTTFAMNLCENAAMASEKPVLVFSLEMPAEQIMMRMIASLARVDQTKIRTGQNL
DEIEWNKIASVVGMFKQKNNLFIDDSSGLTPTDVRSRARRVYRENGGLSMIMVDYLQLMRAPAFSDNRTLEIAEISRSLK
ALAKELQVPVVALSQLNRTLEQRGDKRPVNSDLRESGSIEQDADLIMFIYRDEVYNDNSEDKGVAEIIIGKQRNGPIGRV
RLKFNGQFSRFDNLAEQREYRDDY

Sequences:

>Translated_504_residues
MDFSTVFYFLFSPHFSLKSNRYLRYHRRHSSIEISPMASQPQIKSSDKKTAQVSIPPHSTEAEQAVLGGIMLSNQHWDGI
AERVIADDFYTFQHRLIFTEMEHLMRNQSPIDLITLDQALRSRGVSDEVGGFAYLAELSNNTPNAINILAYADIVREKAI
LRELISVGNRIAENSYSPKGQDIKLILDEAEREVFAIAEKRTTSSEGPQNVINVLESTIEKIDILSKLENHSGVTGVTTG
FTDLDKKTAGLQPSDLIIVAARPSMGKTTFAMNLCENAAMASEKPVLVFSLEMPAEQIMMRMIASLARVDQTKIRTGQNL
DEIEWNKIASVVGMFKQKNNLFIDDSSGLTPTDVRSRARRVYRENGGLSMIMVDYLQLMRAPAFSDNRTLEIAEISRSLK
ALAKELQVPVVALSQLNRTLEQRGDKRPVNSDLRESGSIEQDADLIMFIYRDEVYNDNSEDKGVAEIIIGKQRNGPIGRV
RLKFNGQFSRFDNLAEQREYRDDY
>Mature_504_residues
MDFSTVFYFLFSPHFSLKSNRYLRYHRRHSSIEISPMASQPQIKSSDKKTAQVSIPPHSTEAEQAVLGGIMLSNQHWDGI
AERVIADDFYTFQHRLIFTEMEHLMRNQSPIDLITLDQALRSRGVSDEVGGFAYLAELSNNTPNAINILAYADIVREKAI
LRELISVGNRIAENSYSPKGQDIKLILDEAEREVFAIAEKRTTSSEGPQNVINVLESTIEKIDILSKLENHSGVTGVTTG
FTDLDKKTAGLQPSDLIIVAARPSMGKTTFAMNLCENAAMASEKPVLVFSLEMPAEQIMMRMIASLARVDQTKIRTGQNL
DEIEWNKIASVVGMFKQKNNLFIDDSSGLTPTDVRSRARRVYRENGGLSMIMVDYLQLMRAPAFSDNRTLEIAEISRSLK
ALAKELQVPVVALSQLNRTLEQRGDKRPVNSDLRESGSIEQDADLIMFIYRDEVYNDNSEDKGVAEIIIGKQRNGPIGRV
RLKFNGQFSRFDNLAEQREYRDDY

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=456, Percent_Identity=69.5175438596491, Blast_Score=650, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 56812; Mature: 56812

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFSTVFYFLFSPHFSLKSNRYLRYHRRHSSIEISPMASQPQIKSSDKKTAQVSIPPHST
CCHHHEEHHHHCCCCCCCCCCHHHHHHCCCCEEEECCCCCCCCCCCCCCEEEEECCCCCC
EAEQAVLGGIMLSNQHWDGIAERVIADDFYTFQHRLIFTEMEHLMRNQSPIDLITLDQAL
CHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHH
RSRGVSDEVGGFAYLAELSNNTPNAINILAYADIVREKAILRELISVGNRIAENSYSPKG
HHCCCCCCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
QDIKLILDEAEREVFAIAEKRTTSSEGPQNVINVLESTIEKIDILSKLENHSGVTGVTTG
CCEEEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECC
FTDLDKKTAGLQPSDLIIVAARPSMGKTTFAMNLCENAAMASEKPVLVFSLEMPAEQIMM
CCCCCHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHH
RMIASLARVDQTKIRTGQNLDEIEWNKIASVVGMFKQKNNLFIDDSSGLTPTDVRSRARR
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHH
VYRENGGLSMIMVDYLQLMRAPAFSDNRTLEIAEISRSLKALAKELQVPVVALSQLNRTL
HHHHCCCEEEHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
EQRGDKRPVNSDLRESGSIEQDADLIMFIYRDEVYNDNSEDKGVAEIIIGKQRNGPIGRV
HHCCCCCCCCHHHHHCCCCCCCCCEEEEEEEHHHCCCCCCCCCCEEEEEECCCCCCEEEE
RLKFNGQFSRFDNLAEQREYRDDY
EEEECCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDFSTVFYFLFSPHFSLKSNRYLRYHRRHSSIEISPMASQPQIKSSDKKTAQVSIPPHST
CCHHHEEHHHHCCCCCCCCCCHHHHHHCCCCEEEECCCCCCCCCCCCCCEEEEECCCCCC
EAEQAVLGGIMLSNQHWDGIAERVIADDFYTFQHRLIFTEMEHLMRNQSPIDLITLDQAL
CHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHH
RSRGVSDEVGGFAYLAELSNNTPNAINILAYADIVREKAILRELISVGNRIAENSYSPKG
HHCCCCCCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
QDIKLILDEAEREVFAIAEKRTTSSEGPQNVINVLESTIEKIDILSKLENHSGVTGVTTG
CCEEEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECC
FTDLDKKTAGLQPSDLIIVAARPSMGKTTFAMNLCENAAMASEKPVLVFSLEMPAEQIMM
CCCCCHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHH
RMIASLARVDQTKIRTGQNLDEIEWNKIASVVGMFKQKNNLFIDDSSGLTPTDVRSRARR
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHH
VYRENGGLSMIMVDYLQLMRAPAFSDNRTLEIAEISRSLKALAKELQVPVVALSQLNRTL
HHHHCCCEEEHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
EQRGDKRPVNSDLRESGSIEQDADLIMFIYRDEVYNDNSEDKGVAEIIIGKQRNGPIGRV
HHCCCCCCCCHHHHHCCCCCCCCCEEEEEEEHHHCCCCCCCCCCEEEEEECCCCCCEEEE
RLKFNGQFSRFDNLAEQREYRDDY
EEEECCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]