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The map label for this gene is bioF [C]

Identifier: 16273453

GI number: 16273453

Start: 1623262

End: 1624404

Strand: Reverse

Name: bioF [C]

Synonym: HI1553

Alternate gene names: 16273453

Gene position: 1624404-1623262 (Counterclockwise)

Preceding gene: 16273454

Following gene: 16273452

Centisome position: 88.76

GC content: 40.51

Gene sequence:

>1143_bases
ATGGATGCTTTCAAACAGCAACTGGAACAGCTTAGCGCAAAAAATCAATATCGTTCAATTCCAGATTTAGTTCATCAAGG
ACGATATATTACGCGAGAAAATCGCAAAATGCTGAATATGTCATCTAATGATTATTTAGGTTTGGCATCAAATGAAAACT
TGCGCCAGTCTTTTTTACAGCAATATGGCGGTAATTTTCCCTCTTTTACCAGTTCTTCATCGCGTTTATTAACGGGCAAC
TTTCCTATTTATACCGATTTGGAAGAGCTTGTCGCACAACGTTTCCAACGGGAAAGTGCGTTATTATTTAACAGCGGCTA
TCACGCCAATATCGGCATTTTGCCTGCTTTGACGACGACGAAAAGTTTGATTTTGGCAGATAAATTAGTGCATGCCAGCA
TGATTGATGGCATTCGTTTAAGCCAATGTGAGTTCTTTCGTTATCGTCATAATGATTATGAACATTTGAAAAATCTGCTT
GAAAAAAACGTCGGAAAATTTGACCGCACTTTTATCGTTACCGAATCTGTTTTCAGTATGGACGGCGACGTGGCGGATTT
GAAACAGCTTGTTCAATTAAAAAAACAATTTCCCAATACTTATCTTTATGTGGATGAAGCCCACGCCGTCGGTGTTTATG
GGCAAAACGGATTGGGTATTGCCGAACGAGCTAATGTGATAGCTGATATAGATTTGTTGGTTGGCACTTTTGGCAAGGCC
TTAGCTTCAATGGGGGCTTATGTCGTCTGTGATCAAATATTAAAAGAATGTTTGATTAATCAAATGCGTCCATTGATTTT
TTCAACCGCACTTCCGCCCTTTAATGTGGCGTGGACTCATTTTATTTTTGAACGATTACCACAACTTTCAAAAGAAAGAA
CGCATCTTGAGCAGTTAAGTGCATTTTTACGCCAAGAAGTGGAACACAGAACGCAAATAATGCCAAGCCAAACTTGCATC
GTTCCCTATATTTTAGGCGAGAACGAAGCAACCCTTGCTAAGGCGAAAGATCTGCAAGAGCAAGGTTATTATTGTTTGCC
AATCAGACCGCCGACAGTACCCAAAAACACATCCAGAATCCGCCTGTCTCTAACTGCCGATATGACGGCAGATGAAGTGC
GGCAGTTTGCGGTGCATTTATAA

Upstream 100 bases:

>100_bases
ATGTTATGCCACCATTTGTGATTAAAGATGATGAACTTCAGAAATTAACGGAAGGCATGATTCTGGCTTTAACTCAGGAA
TATGAACATTAGGATAAAAA

Downstream 100 bases:

>100_bases
ATATATGATATGAAAACGAAATTTTACGATTACCAAGGCGAACATTTAATCCTGTATTTTGCAGGTTGGGGAACGCCGCC
CGATGCTGTAAATCATTTGA

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS/AKB ligase; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; Alpha-oxoamine synthase; L-alanine--pimeloyl-CoA ligase [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MDAFKQQLEQLSAKNQYRSIPDLVHQGRYITRENRKMLNMSSNDYLGLASNENLRQSFLQQYGGNFPSFTSSSSRLLTGN
FPIYTDLEELVAQRFQRESALLFNSGYHANIGILPALTTTKSLILADKLVHASMIDGIRLSQCEFFRYRHNDYEHLKNLL
EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAVGVYGQNGLGIAERANVIADIDLLVGTFGKA
LASMGAYVVCDQILKECLINQMRPLIFSTALPPFNVAWTHFIFERLPQLSKERTHLEQLSAFLRQEVEHRTQIMPSQTCI
VPYILGENEATLAKAKDLQEQGYYCLPIRPPTVPKNTSRIRLSLTADMTADEVRQFAVHL

Sequences:

>Translated_380_residues
MDAFKQQLEQLSAKNQYRSIPDLVHQGRYITRENRKMLNMSSNDYLGLASNENLRQSFLQQYGGNFPSFTSSSSRLLTGN
FPIYTDLEELVAQRFQRESALLFNSGYHANIGILPALTTTKSLILADKLVHASMIDGIRLSQCEFFRYRHNDYEHLKNLL
EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAVGVYGQNGLGIAERANVIADIDLLVGTFGKA
LASMGAYVVCDQILKECLINQMRPLIFSTALPPFNVAWTHFIFERLPQLSKERTHLEQLSAFLRQEVEHRTQIMPSQTCI
VPYILGENEATLAKAKDLQEQGYYCLPIRPPTVPKNTSRIRLSLTADMTADEVRQFAVHL
>Mature_380_residues
MDAFKQQLEQLSAKNQYRSIPDLVHQGRYITRENRKMLNMSSNDYLGLASNENLRQSFLQQYGGNFPSFTSSSSRLLTGN
FPIYTDLEELVAQRFQRESALLFNSGYHANIGILPALTTTKSLILADKLVHASMIDGIRLSQCEFFRYRHNDYEHLKNLL
EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAVGVYGQNGLGIAERANVIADIDLLVGTFGKA
LASMGAYVVCDQILKECLINQMRPLIFSTALPPFNVAWTHFIFERLPQLSKERTHLEQLSAFLRQEVEHRTQIMPSQTCI
VPYILGENEATLAKAKDLQEQGYYCLPIRPPTVPKNTSRIRLSLTADMTADEVRQFAVHL

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A and/or converts 2-amino-3-ketobutyrate to glycine and acetyl-CoA [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI7657118, Length=381, Percent_Identity=30.9711286089239, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI284448556, Length=345, Percent_Identity=32.463768115942, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI83977444, Length=337, Percent_Identity=33.8278931750742, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI83977442, Length=350, Percent_Identity=32.8571428571429, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI83977440, Length=337, Percent_Identity=33.8278931750742, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI119220554, Length=370, Percent_Identity=27.2972972972973, Blast_Score=138, Evalue=7e-33,
Organism=Homo sapiens, GI4758668, Length=358, Percent_Identity=27.9329608938547, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI4502025, Length=346, Percent_Identity=28.9017341040462, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI40316939, Length=346, Percent_Identity=28.9017341040462, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI5454084, Length=253, Percent_Identity=25.6916996047431, Blast_Score=99, Evalue=6e-21,
Organism=Escherichia coli, GI1786993, Length=343, Percent_Identity=35.8600583090379, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1790046, Length=344, Percent_Identity=31.3953488372093, Blast_Score=177, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI71994529, Length=386, Percent_Identity=32.1243523316062, Blast_Score=175, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI32566772, Length=364, Percent_Identity=25.8241758241758, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17560912, Length=241, Percent_Identity=31.9502074688797, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17560914, Length=240, Percent_Identity=32.0833333333333, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71982617, Length=262, Percent_Identity=26.7175572519084, Blast_Score=101, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI71982625, Length=262, Percent_Identity=26.7175572519084, Blast_Score=100, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6320438, Length=374, Percent_Identity=30.4812834224599, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6320267, Length=362, Percent_Identity=26.7955801104972, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6323954, Length=295, Percent_Identity=28.135593220339, Blast_Score=113, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24662918, Length=345, Percent_Identity=34.2028985507246, Blast_Score=192, Evalue=4e-49,
Organism=Drosophila melanogaster, GI17137420, Length=358, Percent_Identity=31.5642458100559, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI17136286, Length=370, Percent_Identity=27.2972972972973, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24653280, Length=288, Percent_Identity=27.4305555555556, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24653276, Length=288, Percent_Identity=27.4305555555556, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24653278, Length=288, Percent_Identity=27.4305555555556, Blast_Score=108, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.3.1.29; =2.3.1.47 [H]

Molecular weight: Translated: 43213; Mature: 43213

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAFKQQLEQLSAKNQYRSIPDLVHQGRYITRENRKMLNMSSNDYLGLASNENLRQSFLQ
CCHHHHHHHHHHHHHHHCCCHHHHHCCCEEECCCCCEECCCCCCEEEECCCCHHHHHHHH
QYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALLFNSGYHANIGILPALTTT
HHCCCCCCCCCCCCEEEECCCCCEECHHHHHHHHHHHHHHEEEECCCCCCCEEEECHHHH
KSLILADKLVHASMIDGIRLSQCEFFRYRHNDYEHLKNLLEKNVGKFDRTFIVTESVFSM
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEHHHHHC
DGDVADLKQLVQLKKQFPNTYLYVDEAHAVGVYGQNGLGIAERANVIADIDLLVGTFGKA
CCCHHHHHHHHHHHHHCCCEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
LASMGAYVVCDQILKECLINQMRPLIFSTALPPFNVAWTHFIFERLPQLSKERTHLEQLS
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AFLRQEVEHRTQIMPSQTCIVPYILGENEATLAKAKDLQEQGYYCLPIRPPTVPKNTSRI
HHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCEE
RLSLTADMTADEVRQFAVHL
EEEEEECCCHHHHHHHHHCC
>Mature Secondary Structure
MDAFKQQLEQLSAKNQYRSIPDLVHQGRYITRENRKMLNMSSNDYLGLASNENLRQSFLQ
CCHHHHHHHHHHHHHHHCCCHHHHHCCCEEECCCCCEECCCCCCEEEECCCCHHHHHHHH
QYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALLFNSGYHANIGILPALTTT
HHCCCCCCCCCCCCEEEECCCCCEECHHHHHHHHHHHHHHEEEECCCCCCCEEEECHHHH
KSLILADKLVHASMIDGIRLSQCEFFRYRHNDYEHLKNLLEKNVGKFDRTFIVTESVFSM
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEHHHHHC
DGDVADLKQLVQLKKQFPNTYLYVDEAHAVGVYGQNGLGIAERANVIADIDLLVGTFGKA
CCCHHHHHHHHHHHHHCCCEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
LASMGAYVVCDQILKECLINQMRPLIFSTALPPFNVAWTHFIFERLPQLSKERTHLEQLS
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AFLRQEVEHRTQIMPSQTCIVPYILGENEATLAKAKDLQEQGYYCLPIRPPTVPKNTSRI
HHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCEE
RLSLTADMTADEVRQFAVHL
EEEEEECCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA