Click here to switch to the map view.

The map label for this gene is bioD

Identifier: 16273450

GI number: 16273450

Start: 1621250

End: 1621825

Strand: Reverse

Name: bioD

Synonym: HI1550

Alternate gene names: 16273450

Gene position: 1621825-1621250 (Counterclockwise)

Preceding gene: 16273451

Following gene: 16273449

Centisome position: 88.62

GC content: 35.59

Gene sequence:

>576_bases
ATGGGAAAGGTTATTTTTATATCTGGCATTGATACTGATGTGGGAAAAACGATTGCAACAGGCATTTATGCTAAAAAGTT
GATGGAACAAGGCTGTTCCGTGATTACACAAAAAATGATTCAAACAGGCTGTAAAAATATTGCTGATGATTTGCTTGTAC
ATCGAAAGATTCAAGGGATTGATTTAACGGAAGAAGATTTACAAGGCAAAACCTGCCCTTATGTTTTTGAATATCCTTGT
TCGCCACATTTAGCTGCAAAGCGAGAAAGTAGAAAAATTGAAGCAAAAATCATTGAAAAATCTACCGCACTTTTGGCAGA
AAAATATGATTATGTACTATTGGAGGGTGCTGGTGGCTTAATGGTACCTTATTGTGAAGAGGCGACGACGTTGGATTATA
TCCAGTTAAATAATTACCCTTTAATTTTAGTGACATCGGGAAAACTAGGCAGTATAAACCACACATTATTAAGTTTAGAG
GCTTGTCGTACTCGAGGTATTTCAGTATTAAGTGTAATGTATAACGGTTACCCAGAATATGACCTATTATTAGTCAAGAA
ACCCAACGCTATTTAA

Upstream 100 bases:

>100_bases
GTCGGCGTTTGGTATGCCGTCGGGCAAAGTGCGCCTGACTTATCATCCGTTATTTTTTATCGCACGCTATTCTCACATAG
AAAATCAGTAAGGAATATTT

Downstream 100 bases:

>100_bases
AACGTTATCTTGAAAAACATTTTCCTGAAACAGGATTTGAATACTTTAGGTGTATTGAAATTTAAGCTCAAGGAAAAGTG
TGATAGACTCGTGCAAGTTT

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase 2; DTBS 2; Dethiobiotin synthase 2

Number of amino acids: Translated: 191; Mature: 190

Protein sequence:

>191_residues
MGKVIFISGIDTDVGKTIATGIYAKKLMEQGCSVITQKMIQTGCKNIADDLLVHRKIQGIDLTEEDLQGKTCPYVFEYPC
SPHLAAKRESRKIEAKIIEKSTALLAEKYDYVLLEGAGGLMVPYCEEATTLDYIQLNNYPLILVTSGKLGSINHTLLSLE
ACRTRGISVLSVMYNGYPEYDLLLVKKPNAI

Sequences:

>Translated_191_residues
MGKVIFISGIDTDVGKTIATGIYAKKLMEQGCSVITQKMIQTGCKNIADDLLVHRKIQGIDLTEEDLQGKTCPYVFEYPC
SPHLAAKRESRKIEAKIIEKSTALLAEKYDYVLLEGAGGLMVPYCEEATTLDYIQLNNYPLILVTSGKLGSINHTLLSLE
ACRTRGISVLSVMYNGYPEYDLLLVKKPNAI
>Mature_190_residues
GKVIFISGIDTDVGKTIATGIYAKKLMEQGCSVITQKMIQTGCKNIADDLLVHRKIQGIDLTEEDLQGKTCPYVFEYPCS
PHLAAKRESRKIEAKIIEKSTALLAEKYDYVLLEGAGGLMVPYCEEATTLDYIQLNNYPLILVTSGKLGSINHTLLSLEA
CRTRGISVLSVMYNGYPEYDLLLVKKPNAI

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family

Homologues:

Organism=Escherichia coli, GI1786995, Length=179, Percent_Identity=30.7262569832402, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87081944, Length=182, Percent_Identity=28.021978021978, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOD2_HAEIN (P45248)

Other databases:

- EMBL:   L42023
- PIR:   B64129
- RefSeq:   NP_439699.1
- ProteinModelPortal:   P45248
- SMR:   P45248
- GeneID:   950414
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1550
- NMPDR:   fig|71421.1.peg.1477
- TIGR:   HI_1550
- HOGENOM:   HBG650065
- OMA:   IDTGIGK
- ProtClustDB:   PRK00090
- BioCyc:   HINF71421:HI_1550-MONOMER
- BRENDA:   6.3.3.3
- HAMAP:   MF_00336
- InterPro:   IPR004472
- InterPro:   IPR002586
- PIRSF:   PIRSF006755
- TIGRFAMs:   TIGR00347

Pfam domain/function: PF01656 CbiA

EC number: =6.3.3.3

Molecular weight: Translated: 21097; Mature: 20966

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKVIFISGIDTDVGKTIATGIYAKKLMEQGCSVITQKMIQTGCKNIADDLLVHRKIQGI
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DLTEEDLQGKTCPYVFEYPCSPHLAAKRESRKIEAKIIEKSTALLAEKYDYVLLEGAGGL
CCCHHHCCCCCCCEEEECCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCCCE
MVPYCEEATTLDYIQLNNYPLILVTSGKLGSINHTLLSLEACRTRGISVLSVMYNGYPEY
EEECCCCCCEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCE
DLLLVKKPNAI
EEEEEECCCCC
>Mature Secondary Structure 
GKVIFISGIDTDVGKTIATGIYAKKLMEQGCSVITQKMIQTGCKNIADDLLVHRKIQGI
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DLTEEDLQGKTCPYVFEYPCSPHLAAKRESRKIEAKIIEKSTALLAEKYDYVLLEGAGGL
CCCHHHCCCCCCCEEEECCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCCCE
MVPYCEEATTLDYIQLNNYPLILVTSGKLGSINHTLLSLEACRTRGISVLSVMYNGYPEY
EEECCCCCCEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCE
DLLLVKKPNAI
EEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800