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The map label for this gene is rfaE [H]

Identifier: 16273426

GI number: 16273426

Start: 1595753

End: 1597183

Strand: Reverse

Name: rfaE [H]

Synonym: HI1526

Alternate gene names: 16273426

Gene position: 1597183-1595753 (Counterclockwise)

Preceding gene: 16273431

Following gene: 16273425

Centisome position: 87.27

GC content: 40.39

Gene sequence:

>1431_bases
ATGGCTCAATATTCAGCAGAATTTAAGCAAGCAAAAGTACTCGTATTAGGCGACGTGATGCTTGATCGTTATTGGTTCGG
CGCAACCAACCGTATTTCACCAGAAGCACCAGTACCAGTGGTTCGTGTACAAGAAAATGAAGAACGCGCGGGTGGTGCAG
CAAATGTGGCGATGAATATTGCTTCACTCAATGTACCCGTTCAGTTAATGGGATTGATTGGACAAGATGAAACTGGTTCT
GCACTTTCCCTCTTATTAGAAAAACAAAAAATTGATTGTAATTTTGTTGCATTAGAAACCCATCCAACCATTACTAAATT
ACGTATTTTATCTCGTCATCAACAGCTGCTCCGCCTTGATTTTGAAGAAGATTTCAATAATGTAGATTGCAAGGATTTAT
TAGCGAAGTTAGAAAGTGCGGTGAAAAATTACGGTGCTTTGATTCTTTCTGATTACGGCAAAGGCACGCTTAAAGATGTT
CAGAAAATGATTCAAATTGCACGCAAAGCGAATGTGCCTGTGTTGATCGATCCAAAGGGAACTGATTTTGAACGTTATCG
TGGGGCTACATTATTGACACCCAATATGTCTGAATTTGAAGCTGTTGTGGGTAAATGCAATACGGAAGAAGAGATTATTG
AGAAAGGTTTAAAATTAATTTCTGATATTGAATTAACCGCACTTTTGGTGACGCGTTCTGAAAAAGGCATGACATTATTA
CGCCCAAATCAAGAGCCTTATCATTTGCCAACTGTTGCAAAAGAAGTGTTTGATGTGACGGGAGCCGGTGACACTGTCAT
TAGCGTATTAGCAACCGCATTAGCAGATGGACGTTCTTTCGAAGAATCTTGTTACCTAGCCAATGTTGCCGCAGGAATTG
TGGTGGGGAAATTGGGGACTTCAACGGTTTCGACCGTGGAACTTGAAAATGCGATTCATGCTCGTCCTGAAACTGGATTT
GGCATTATGAGTGAAGCAGAATTAAAAGATGCTGTCGCACAAGCTAAAGCGCGCGGTGAAAAAATTGTGATGACTAATGG
CTGTTTTGATATTTTGCATCCAGGGCATATTTCTTATTTAGAAAATGCACGCAAATTGGGCGATCGTCTAATTGTTGCGG
TAAACAGCGACGATTCTGTTAAACGCTTAAAAGGTGAAAGTCGCCCAATTAATAATCTTGAAAACCGTATGGCGGTATTG
GCTGGTTTGGCATCCGTAGACTGGTTGGTGCCTTTCACTGAAGATACACCACAACGTTTAATCGGCGAAATTCTACCAGA
TCTTTTAGTCAAAGGCGGCGATTACAAACCCGAAGAGATTGCAGGCAGTAAAGAAGTTTGGGCAAACGGTGGCGATGTTA
AAGTGCTAAACTTTGAAAATGGTTGTTCAACAACAAATGTGATTGAAAAAATTAAATTATTGAAAGATTAA

Upstream 100 bases:

>100_bases
TCATTGTTCTTTCCACGTAAGTTAGGGGCGTAGTTTATCGTAAATTTGAGTTGTGGTAAAATCGCCACAATTTTGGTTAT
TCAAATAGAGAGATTTTAAA

Downstream 100 bases:

>100_bases
GTGAAAGGTTGGGGAGTCCCAACCTTTTTTGTTGTTTCAAAATTAAGTCAATTAGTGCCATGTCATGAAATTAGAAAAAA
AATCGATTAATTTATAAACT

Product: bifunctional heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MAQYSAEFKQAKVLVLGDVMLDRYWFGATNRISPEAPVPVVRVQENEERAGGAANVAMNIASLNVPVQLMGLIGQDETGS
ALSLLLEKQKIDCNFVALETHPTITKLRILSRHQQLLRLDFEEDFNNVDCKDLLAKLESAVKNYGALILSDYGKGTLKDV
QKMIQIARKANVPVLIDPKGTDFERYRGATLLTPNMSEFEAVVGKCNTEEEIIEKGLKLISDIELTALLVTRSEKGMTLL
RPNQEPYHLPTVAKEVFDVTGAGDTVISVLATALADGRSFEESCYLANVAAGIVVGKLGTSTVSTVELENAIHARPETGF
GIMSEAELKDAVAQAKARGEKIVMTNGCFDILHPGHISYLENARKLGDRLIVAVNSDDSVKRLKGESRPINNLENRMAVL
AGLASVDWLVPFTEDTPQRLIGEILPDLLVKGGDYKPEEIAGSKEVWANGGDVKVLNFENGCSTTNVIEKIKLLKD

Sequences:

>Translated_476_residues
MAQYSAEFKQAKVLVLGDVMLDRYWFGATNRISPEAPVPVVRVQENEERAGGAANVAMNIASLNVPVQLMGLIGQDETGS
ALSLLLEKQKIDCNFVALETHPTITKLRILSRHQQLLRLDFEEDFNNVDCKDLLAKLESAVKNYGALILSDYGKGTLKDV
QKMIQIARKANVPVLIDPKGTDFERYRGATLLTPNMSEFEAVVGKCNTEEEIIEKGLKLISDIELTALLVTRSEKGMTLL
RPNQEPYHLPTVAKEVFDVTGAGDTVISVLATALADGRSFEESCYLANVAAGIVVGKLGTSTVSTVELENAIHARPETGF
GIMSEAELKDAVAQAKARGEKIVMTNGCFDILHPGHISYLENARKLGDRLIVAVNSDDSVKRLKGESRPINNLENRMAVL
AGLASVDWLVPFTEDTPQRLIGEILPDLLVKGGDYKPEEIAGSKEVWANGGDVKVLNFENGCSTTNVIEKIKLLKD
>Mature_475_residues
AQYSAEFKQAKVLVLGDVMLDRYWFGATNRISPEAPVPVVRVQENEERAGGAANVAMNIASLNVPVQLMGLIGQDETGSA
LSLLLEKQKIDCNFVALETHPTITKLRILSRHQQLLRLDFEEDFNNVDCKDLLAKLESAVKNYGALILSDYGKGTLKDVQ
KMIQIARKANVPVLIDPKGTDFERYRGATLLTPNMSEFEAVVGKCNTEEEIIEKGLKLISDIELTALLVTRSEKGMTLLR
PNQEPYHLPTVAKEVFDVTGAGDTVISVLATALADGRSFEESCYLANVAAGIVVGKLGTSTVSTVELENAIHARPETGFG
IMSEAELKDAVAQAKARGEKIVMTNGCFDILHPGHISYLENARKLGDRLIVAVNSDDSVKRLKGESRPINNLENRMAVLA
GLASVDWLVPFTEDTPQRLIGEILPDLLVKGGDYKPEEIAGSKEVWANGGDVKVLNFENGCSTTNVIEKIKLLKD

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose [H]

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789432, Length=466, Percent_Identity=71.0300429184549, Blast_Score=691, Evalue=0.0,
Organism=Drosophila melanogaster, GI45550975, Length=209, Percent_Identity=29.1866028708134, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45550974, Length=214, Percent_Identity=28.5046728971963, Blast_Score=66, Evalue=6e-11,
Organism=Drosophila melanogaster, GI45550973, Length=64, Percent_Identity=50, Blast_Score=66, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB [H]

EC number: 2.7.-.-

Molecular weight: Translated: 51946; Mature: 51815

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQYSAEFKQAKVLVLGDVMLDRYWFGATNRISPEAPVPVVRVQENEERAGGAANVAMNI
CCCCCCCHHHCEEEEEHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCHHHEEEEE
ASLNVPVQLMGLIGQDETGSALSLLLEKQKIDCNFVALETHPTITKLRILSRHQQLLRLD
EECCCCCEEHEECCCCCCCHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHCC
FEEDFNNVDCKDLLAKLESAVKNYGALILSDYGKGTLKDVQKMIQIARKANVPVLIDPKG
HHHCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEECCCC
TDFERYRGATLLTPNMSEFEAVVGKCNTEEEIIEKGLKLISDIELTALLVTRSEKGMTLL
CCHHHHCCCEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCEEE
RPNQEPYHLPTVAKEVFDVTGAGDTVISVLATALADGRSFEESCYLANVAAGIVVGKLGT
CCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEECCCC
STVSTVELENAIHARPETGFGIMSEAELKDAVAQAKARGEKIVMTNGCFDILHPGHISYL
CCEEEEEHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCHHHCCCCHHHHH
ENARKLGDRLIVAVNSDDSVKRLKGESRPINNLENRMAVLAGLASVDWLVPFTEDTPQRL
HHHHHCCCEEEEEECCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCEEEECCCCCHHHH
IGEILPDLLVKGGDYKPEEIAGSKEVWANGGDVKVLNFENGCSTTNVIEKIKLLKD
HHHHHHHHHHCCCCCCHHHHCCCHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
AQYSAEFKQAKVLVLGDVMLDRYWFGATNRISPEAPVPVVRVQENEERAGGAANVAMNI
CCCCCCHHHCEEEEEHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCHHHEEEEE
ASLNVPVQLMGLIGQDETGSALSLLLEKQKIDCNFVALETHPTITKLRILSRHQQLLRLD
EECCCCCEEHEECCCCCCCHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHCC
FEEDFNNVDCKDLLAKLESAVKNYGALILSDYGKGTLKDVQKMIQIARKANVPVLIDPKG
HHHCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEECCCC
TDFERYRGATLLTPNMSEFEAVVGKCNTEEEIIEKGLKLISDIELTALLVTRSEKGMTLL
CCHHHHCCCEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCEEE
RPNQEPYHLPTVAKEVFDVTGAGDTVISVLATALADGRSFEESCYLANVAAGIVVGKLGT
CCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEECCCC
STVSTVELENAIHARPETGFGIMSEAELKDAVAQAKARGEKIVMTNGCFDILHPGHISYL
CCEEEEEHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCHHHCCCCHHHHH
ENARKLGDRLIVAVNSDDSVKRLKGESRPINNLENRMAVLAGLASVDWLVPFTEDTPQRL
HHHHHCCCEEEEEECCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCEEEECCCCCHHHH
IGEILPDLLVKGGDYKPEEIAGSKEVWANGGDVKVLNFENGCSTTNVIEKIKLLKD
HHHHHHHHHHCCCCCCHHHHCCCHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA