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The map label for this gene is metF

Identifier: 16273351

GI number: 16273351

Start: 1531847

End: 1532725

Strand: Direct

Name: metF

Synonym: HI1444

Alternate gene names: 16273351

Gene position: 1531847-1532725 (Clockwise)

Preceding gene: 16273346

Following gene: 16273355

Centisome position: 83.7

GC content: 36.63

Gene sequence:

>879_bases
ATGAGCTACGCGAAAGAAATTGATACATTAAATCAACATATTGCAGATTTTAATAAAAAAATTAATGTCTCCTTTGAATT
TTTTCCACCTAAAAACGAAAAAATGGAAACCCTTCTATGGGATTCAATTCATCGTTTAAAAGTATTAAAGCCTAAATTTG
TGTCAGTCACTTACGGTGCAAATTCGGGAGAACGTGACCGCACTCACGGCATTGTGAAAGCCATTAAACAAGAAACTGGC
TTAGAAGCCGCACCACATTTAACTGGAATTGATGCCACACCTGAAGAATTAAAACAAATTGCGAGAGATTATTGGGATAG
TGGTATTCGCCGTATTGTTGCGTTACGCGGTGACGAACCTAAAGGTTACGCGAAAAAACCATTTTATGCGTCAGATCTTG
TGGAATTACTCCGTTCTGTCGCTGATTTTGATATTTCTGTAGCCGCTTATCCCGAAGTTCATCCAGAAGCAAAATCCGCA
CAAGCAGACTTAATTAATTTAAAACGTAAAATTGATGCAGGTGCAAACCACGTCATTACACAATTTTTCTTTGATATTGA
AAACTACCTACGTTTTCGTGATCGTTGTGCATCAATTGGTATTGATACTGAAATCGTACCCGGTATTTTACCTGTTACTA
ATTTTAAACAACTCCAAAAAATGGCATCATTCACTAATGTGAAAATTCCAGCGTGGTTAGTTAAAGCCTATGATGGTTTG
GATAATGATCCAACTACACGTAATCTTGTGGCAGCAAGTGTTGCAATGGATATGGTAAAAATTTTATCTCGCGAAGGCGT
GAATGACTTCCACTTTTATACATTAAATCGTAGTGAATTAACTTATGCTATCTGTCATATGTTAGGTGTAAGACCTTAA

Upstream 100 bases:

>100_bases
ATTAATAATAATCATACTTTTAATGAATAAATTGAAATTGACTCATATTAAAACCTATTTTATTGTACAAATACTATTTT
AGAATCCTAAAGGAAGCCTT

Downstream 100 bases:

>100_bases
TTATCACAATAATATATTTCTTTTCAAACACAAAATAAGGGCGATATTTCGCCCTTATACAAAACTAAAAAAATTCAACC
GCACTTTATTATTTTATGAA

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MSYAKEIDTLNQHIADFNKKINVSFEFFPPKNEKMETLLWDSIHRLKVLKPKFVSVTYGANSGERDRTHGIVKAIKQETG
LEAAPHLTGIDATPEELKQIARDYWDSGIRRIVALRGDEPKGYAKKPFYASDLVELLRSVADFDISVAAYPEVHPEAKSA
QADLINLKRKIDAGANHVITQFFFDIENYLRFRDRCASIGIDTEIVPGILPVTNFKQLQKMASFTNVKIPAWLVKAYDGL
DNDPTTRNLVAASVAMDMVKILSREGVNDFHFYTLNRSELTYAICHMLGVRP

Sequences:

>Translated_292_residues
MSYAKEIDTLNQHIADFNKKINVSFEFFPPKNEKMETLLWDSIHRLKVLKPKFVSVTYGANSGERDRTHGIVKAIKQETG
LEAAPHLTGIDATPEELKQIARDYWDSGIRRIVALRGDEPKGYAKKPFYASDLVELLRSVADFDISVAAYPEVHPEAKSA
QADLINLKRKIDAGANHVITQFFFDIENYLRFRDRCASIGIDTEIVPGILPVTNFKQLQKMASFTNVKIPAWLVKAYDGL
DNDPTTRNLVAASVAMDMVKILSREGVNDFHFYTLNRSELTYAICHMLGVRP
>Mature_291_residues
SYAKEIDTLNQHIADFNKKINVSFEFFPPKNEKMETLLWDSIHRLKVLKPKFVSVTYGANSGERDRTHGIVKAIKQETGL
EAAPHLTGIDATPEELKQIARDYWDSGIRRIVALRGDEPKGYAKKPFYASDLVELLRSVADFDISVAAYPEVHPEAKSAQ
ADLINLKRKIDAGANHVITQFFFDIENYLRFRDRCASIGIDTEIVPGILPVTNFKQLQKMASFTNVKIPAWLVKAYDGLD
NDPTTRNLVAASVAMDMVKILSREGVNDFHFYTLNRSELTYAICHMLGVRP

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family

Homologues:

Organism=Homo sapiens, GI87240000, Length=283, Percent_Identity=33.9222614840989, Blast_Score=141, Evalue=6e-34,
Organism=Escherichia coli, GI1790377, Length=290, Percent_Identity=73.1034482758621, Blast_Score=457, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI25147441, Length=278, Percent_Identity=29.8561151079137, Blast_Score=128, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI25147436, Length=278, Percent_Identity=29.8561151079137, Blast_Score=128, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6325234, Length=294, Percent_Identity=32.312925170068, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6321313, Length=301, Percent_Identity=29.2358803986711, Blast_Score=126, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): METF_HAEIN (P45208)

Other databases:

- EMBL:   L42023
- PIR:   H64123
- RefSeq:   NP_439596.1
- ProteinModelPortal:   P45208
- SMR:   P45208
- GeneID:   950344
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1444
- NMPDR:   fig|71421.1.peg.1378
- TIGR:   HI_1444
- HOGENOM:   HBG649727
- OMA:   ITQMFFD
- ProtClustDB:   PRK09432
- BioCyc:   HINF71421:HI_1444-MONOMER
- BRENDA:   1.5.1.20
- InterPro:   IPR003171
- InterPro:   IPR004620
- TIGRFAMs:   TIGR00676

Pfam domain/function: PF02219 MTHFR

EC number: =1.5.1.20

Molecular weight: Translated: 33020; Mature: 32889

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYAKEIDTLNQHIADFNKKINVSFEFFPPKNEKMETLLWDSIHRLKVLKPKFVSVTYGA
CCHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEECC
NSGERDRTHGIVKAIKQETGLEAAPHLTGIDATPEELKQIARDYWDSGIRRIVALRGDEP
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEHHCCCCCC
KGYAKKPFYASDLVELLRSVADFDISVAAYPEVHPEAKSAQADLINLKRKIDAGANHVIT
CCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHH
QFFFDIENYLRFRDRCASIGIDTEIVPGILPVTNFKQLQKMASFTNVKIPAWLVKAYDGL
HHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCC
DNDPTTRNLVAASVAMDMVKILSREGVNDFHFYTLNRSELTYAICHMLGVRP
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SYAKEIDTLNQHIADFNKKINVSFEFFPPKNEKMETLLWDSIHRLKVLKPKFVSVTYGA
CHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEECC
NSGERDRTHGIVKAIKQETGLEAAPHLTGIDATPEELKQIARDYWDSGIRRIVALRGDEP
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEHHCCCCCC
KGYAKKPFYASDLVELLRSVADFDISVAAYPEVHPEAKSAQADLINLKRKIDAGANHVIT
CCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHH
QFFFDIENYLRFRDRCASIGIDTEIVPGILPVTNFKQLQKMASFTNVKIPAWLVKAYDGL
HHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCC
DNDPTTRNLVAASVAMDMVKILSREGVNDFHFYTLNRSELTYAICHMLGVRP
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800