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The map label for this gene is fumC

Identifier: 16273307

GI number: 16273307

Start: 1494535

End: 1495929

Strand: Direct

Name: fumC

Synonym: HI1398

Alternate gene names: 16273307

Gene position: 1494535-1495929 (Clockwise)

Preceding gene: 16273300

Following gene: 16273308

Centisome position: 81.66

GC content: 39.05

Gene sequence:

>1395_bases
ATGGCATTTCGTATTGAAAAAGACACAATGGGTGAAGTTCAAGTTCTAGCAGATAAATACTGGGCTGCACAAACTGAACG
TTCACGCAATAACTTTAAAATTGGCCCAGCCGCATCAATGCCACATGAAATTATCGAAGCTTTTGGCTATTTGAAAAAAG
CAGCCGCATTTGCTAATCATGATTTGGGTGTATTACCTCTTGAAAAACGTGATTTAATTGCTCTGGCTTGTGACGAAATT
TTAGCAAATAAATTAGATGATCAATTCCCACTTGTTATTTGGCAAACTGGTTCTGGTACACAATCTAATATGAATGTGAA
CGAAGTAGTTGCCAATCGTGCTCATGTGTTAAACGGTGGAAAATTAGGTGAAAAATCTATTATTCACCCAAATGATGATG
TGAACAAATCACAATCTTCAAATGATACTTTCCCAACGGCAATGCACATTGCGGCATATAAAAAAGTAGTTGAGCACACC
ATTCCTTGTGTTGAACGTTTACAAAAAACTTTTGCGGCAAAATCTGAAGCCTTCAAAAACGTGGTAAAAATTGGCCGCAC
TCACTTAATGGATGCAACGCCACTCACATTAGGTCAAGAATTTTCGGCTTATGCAGCTCAATTAGATTTCGGCTTAAAAG
CATTAAAAAATACCCTCCCACATTTAAGCCAATTAGCATTGGGTGGAACTGCGGTTGGCACAGGTTTGAACACGCCGAAA
GGCTATGATTTGAAAGTGGTAGATTACATTGCAAAATTCACCGCACTTCCGTTTGTCACAGCAGATAATAAATTTGAAGC
ATTAGCCGCTCATGATGCGATTGTTGAAACTCACGGTGCATTACGTCAATTAGCAATGAGTTTATTTAAAATTGCGAACG
ATATTCGTCTATTAGCATCTGGCCCTCGTTCGGGAATTGGCGAAATTTTAATTCCTGAAAATGAACCAGGTTCTTCAATT
ATGCCAGGTAAAGTAAACCCAACTCAATGCGAASSGATGACAATGGTCTGTGCACAAGTATTTGGTAACGACACCACCAT
CGCATTTGTGGGTTCACAAGGTCACTTCCAATTAAATGTGTTCAACCCTGTGATGATTGCAAACTTCTTACAATCTGCTC
AATTATTGGGCGATGCTTGCGTATCTTTCGATGAACACTGTGCAGTGGGTATTGAGCCAAACTATCCACGCATTAAACAA
CAACTTGAAAATTCATTGATGTTGGTGACCGCACTTAATACGCATATCGGTTATGAAAATGCAGCTAAAATTGCAAAAAC
CGCACATAAAAATGGCACCACATTACGTGAAGAAGCGATTAATTTAGGTTTAGTGTCTGCCGAAGATTTTGATAAATGGG
TTCGCCCAGAAGATATGGTGGGTAGCTTAAAATAA

Upstream 100 bases:

>100_bases
ACAAAACTGATTTTTAACGTTTTTGTTACGTTCCACTCATAACTCGGAAGTGTTAAAATCACTTGCGTTTTAATTTTCAT
TCAATTCATAGGAGTACAGA

Downstream 100 bases:

>100_bases
TTCATCGCACAAATTAACATTACAACGATCAAAGGCGAGTAAAATTACTCGCCTTTTTACTGATTTCTAATTTGCTTTTT
GATGCGGTTTGTTATAATCA

Product: fumarate hydratase

Products: NA

Alternate protein names: Fumarase C

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MAFRIEKDTMGEVQVLADKYWAAQTERSRNNFKIGPAASMPHEIIEAFGYLKKAAAFANHDLGVLPLEKRDLIALACDEI
LANKLDDQFPLVIWQTGSGTQSNMNVNEVVANRAHVLNGGKLGEKSIIHPNDDVNKSQSSNDTFPTAMHIAAYKKVVEHT
IPCVERLQKTFAAKSEAFKNVVKIGRTHLMDATPLTLGQEFSAYAAQLDFGLKALKNTLPHLSQLALGGTAVGTGLNTPK
GYDLKVVDYIAKFTALPFVTADNKFEALAAHDAIVETHGALRQLAMSLFKIANDIRLLASGPRSGIGEILIPENEPGSSI
MPGKVNPTQCEXMTMVCAQVFGNDTTIAFVGSQGHFQLNVFNPVMIANFLQSAQLLGDACVSFDEHCAVGIEPNYPRIKQ
QLENSLMLVTALNTHIGYENAAKIAKTAHKNGTTLREEAINLGLVSAEDFDKWVRPEDMVGSLK

Sequences:

>Translated_464_residues
MAFRIEKDTMGEVQVLADKYWAAQTERSRNNFKIGPAASMPHEIIEAFGYLKKAAAFANHDLGVLPLEKRDLIALACDEI
LANKLDDQFPLVIWQTGSGTQSNMNVNEVVANRAHVLNGGKLGEKSIIHPNDDVNKSQSSNDTFPTAMHIAAYKKVVEHT
IPCVERLQKTFAAKSEAFKNVVKIGRTHLMDATPLTLGQEFSAYAAQLDFGLKALKNTLPHLSQLALGGTAVGTGLNTPK
GYDLKVVDYIAKFTALPFVTADNKFEALAAHDAIVETHGALRQLAMSLFKIANDIRLLASGPRSGIGEILIPENEPGSSI
MPGKVNPTQCEXMTMVCAQVFGNDTTIAFVGSQGHFQLNVFNPVMIANFLQSAQLLGDACVSFDEHCAVGIEPNYPRIKQ
QLENSLMLVTALNTHIGYENAAKIAKTAHKNGTTLREEAINLGLVSAEDFDKWVRPEDMVGSLK
>Mature_463_residues
AFRIEKDTMGEVQVLADKYWAAQTERSRNNFKIGPAASMPHEIIEAFGYLKKAAAFANHDLGVLPLEKRDLIALACDEIL
ANKLDDQFPLVIWQTGSGTQSNMNVNEVVANRAHVLNGGKLGEKSIIHPNDDVNKSQSSNDTFPTAMHIAAYKKVVEHTI
PCVERLQKTFAAKSEAFKNVVKIGRTHLMDATPLTLGQEFSAYAAQLDFGLKALKNTLPHLSQLALGGTAVGTGLNTPKG
YDLKVVDYIAKFTALPFVTADNKFEALAAHDAIVETHGALRQLAMSLFKIANDIRLLASGPRSGIGEILIPENEPGSSIM
PGKVNPTQCEXMTMVCAQVFGNDTTIAFVGSQGHFQLNVFNPVMIANFLQSAQLLGDACVSFDEHCAVGIEPNYPRIKQQ
LENSLMLVTALNTHIGYENAAKIAKTAHKNGTTLREEAINLGLVSAEDFDKWVRPEDMVGSLK

Specific function: Tricarboxylic acid cycle [C]

COG id: COG0114

COG function: function code C; Fumarase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Fumarase subfamily

Homologues:

Organism=Homo sapiens, GI19743875, Length=461, Percent_Identity=61.6052060737527, Blast_Score=569, Evalue=1e-162,
Organism=Homo sapiens, GI266458395, Length=87, Percent_Identity=45.9770114942529, Blast_Score=72, Evalue=9e-13,
Organism=Escherichia coli, GI1787896, Length=461, Percent_Identity=62.0390455531453, Blast_Score=567, Evalue=1e-163,
Organism=Escherichia coli, GI87082375, Length=461, Percent_Identity=40.5639913232104, Blast_Score=335, Evalue=3e-93,
Organism=Caenorhabditis elegans, GI17553882, Length=462, Percent_Identity=60.8225108225108, Blast_Score=576, Evalue=1e-165,
Organism=Caenorhabditis elegans, GI32565146, Length=325, Percent_Identity=61.8461538461539, Blast_Score=424, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6324993, Length=461, Percent_Identity=59.4360086767896, Blast_Score=546, Evalue=1e-156,
Organism=Drosophila melanogaster, GI24640179, Length=461, Percent_Identity=59.8698481561822, Blast_Score=559, Evalue=1e-159,
Organism=Drosophila melanogaster, GI24640177, Length=461, Percent_Identity=59.8698481561822, Blast_Score=559, Evalue=1e-159,
Organism=Drosophila melanogaster, GI78710009, Length=462, Percent_Identity=58.4415584415584, Blast_Score=551, Evalue=1e-157,
Organism=Drosophila melanogaster, GI24662684, Length=461, Percent_Identity=57.0498915401301, Blast_Score=533, Evalue=1e-152,
Organism=Drosophila melanogaster, GI24583245, Length=461, Percent_Identity=50.5422993492408, Blast_Score=452, Evalue=1e-127,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FUMC_HAEIN (P71384)

Other databases:

- EMBL:   L42023
- RefSeq:   NP_439551.1
- ProteinModelPortal:   P71384
- GeneID:   950314
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1398
- NMPDR:   fig|71421.1.peg.1334
- TIGR:   HI_1398
- HOGENOM:   HBG284369
- OMA:   RIEKDTM
- ProtClustDB:   PRK00485
- BioCyc:   HINF71421:HI_1398-MONOMER
- BRENDA:   4.2.1.2
- HAMAP:   MF_00743
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- PRINTS:   PR00149
- TIGRFAMs:   TIGR00979

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1; SSF48557 L-Aspartase-like

EC number: =4.2.1.2

Molecular weight: Translated: 50428; Mature: 50297

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: BINDING 100-100

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFRIEKDTMGEVQVLADKYWAAQTERSRNNFKIGPAASMPHEIIEAFGYLKKAAAFANH
CCEEECCCCCCHHHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHCC
DLGVLPLEKRDLIALACDEILANKLDDQFPLVIWQTGSGTQSNMNVNEVVANRAHVLNGG
CCCEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHEECCC
KLGEKSIIHPNDDVNKSQSSNDTFPTAMHIAAYKKVVEHTIPCVERLQKTFAAKSEAFKN
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVKIGRTHLMDATPLTLGQEFSAYAAQLDFGLKALKNTLPHLSQLALGGTAVGTGLNTPK
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCC
GYDLKVVDYIAKFTALPFVTADNKFEALAAHDAIVETHGALRQLAMSLFKIANDIRLLAS
CCCHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
GPRSGIGEILIPENEPGSSIMPGKVNPTQCEXMTMVCAQVFGNDTTIAFVGSQGHFQLNV
CCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEE
FNPVMIANFLQSAQLLGDACVSFDEHCAVGIEPNYPRIKQQLENSLMLVTALNTHIGYEN
CCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHCCEEEEEEHHHHCCCHH
AAKIAKTAHKNGTTLREEAINLGLVSAEDFDKWVRPEDMVGSLK
HHHHHHHHCCCCCHHHHHHHHCCEECHHHHHHHCCCHHHHCCCC
>Mature Secondary Structure 
AFRIEKDTMGEVQVLADKYWAAQTERSRNNFKIGPAASMPHEIIEAFGYLKKAAAFANH
CEEECCCCCCHHHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHCC
DLGVLPLEKRDLIALACDEILANKLDDQFPLVIWQTGSGTQSNMNVNEVVANRAHVLNGG
CCCEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHEECCC
KLGEKSIIHPNDDVNKSQSSNDTFPTAMHIAAYKKVVEHTIPCVERLQKTFAAKSEAFKN
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVKIGRTHLMDATPLTLGQEFSAYAAQLDFGLKALKNTLPHLSQLALGGTAVGTGLNTPK
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCC
GYDLKVVDYIAKFTALPFVTADNKFEALAAHDAIVETHGALRQLAMSLFKIANDIRLLAS
CCCHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
GPRSGIGEILIPENEPGSSIMPGKVNPTQCEXMTMVCAQVFGNDTTIAFVGSQGHFQLNV
CCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEE
FNPVMIANFLQSAQLLGDACVSFDEHCAVGIEPNYPRIKQQLENSLMLVTALNTHIGYEN
CCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHCCEEEEEEHHHHCCCHH
AAKIAKTAHKNGTTLREEAINLGLVSAEDFDKWVRPEDMVGSLK
HHHHHHHHCCCCCHHHHHHHHCCEECHHHHHHHCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800