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The map label for this gene is holC

Identifier: 16273306

GI number: 16273306

Start: 1493866

End: 1494300

Strand: Reverse

Name: holC

Synonym: HI1397

Alternate gene names: 16273306

Gene position: 1494300-1493866 (Counterclockwise)

Preceding gene: 16273309

Following gene: 30995446

Centisome position: 81.65

GC content: 38.16

Gene sequence:

>435_bases
ATGGCAAAAACTGCGCAATTTTATATTCTTACGGAAAATTGTACTTTAACTGTCGAAGAAATAGCCTGTAATCTTGCTGC
TTCAATATGGCGTTCAGGGAAAAAAGTACTAATTAGTTGCGAAAGTGAGGCTCAAGCGCTAGAGATTGACGAGCGTTTGT
GGCAACGAGATCCTAATGAATTTGTTCCACATAATTTATCGGGTGAGGCAACACAATATCCCACACCGATTGAGATTTCA
TGGCTTGGAAAACGAAATTTACAACGCCGCGATTTGTTAATCAATTTGCAACAAGAAATCCCAGATTTTAGCCATAGTTT
TACCCAAATTATTGATTTTGTGCCGAAAGATGATGCGTTAAAAACACAAGCTCGAGAACGTTATAAACAATTACGTCTGC
AAGGTTGGAATCTTTCTACTGAAAACGTGGGTTAA

Upstream 100 bases:

>100_bases
CATTTGTAATAATGCATATTTAATTATTTATTTTTTAGGGTTTTTTCGTTAAAATTTTACCCTTTTTATAGGGTAAGATT
GAGAAAATAACGGGGCTAAA

Downstream 100 bases:

>100_bases
TAAATGAACGATATTACCTTTTATCAACGCTTTCAATTTTGAGTATTGAGCAGTAGGTTTTGAATTAATTAAAGAGGATA
ATTGATGACTGAACGTGAGA

Product: DNA polymerase III subunit chi

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 144; Mature: 143

Protein sequence:

>144_residues
MAKTAQFYILTENCTLTVEEIACNLAASIWRSGKKVLISCESEAQALEIDERLWQRDPNEFVPHNLSGEATQYPTPIEIS
WLGKRNLQRRDLLINLQQEIPDFSHSFTQIIDFVPKDDALKTQARERYKQLRLQGWNLSTENVG

Sequences:

>Translated_144_residues
MAKTAQFYILTENCTLTVEEIACNLAASIWRSGKKVLISCESEAQALEIDERLWQRDPNEFVPHNLSGEATQYPTPIEIS
WLGKRNLQRRDLLINLQQEIPDFSHSFTQIIDFVPKDDALKTQARERYKQLRLQGWNLSTENVG
>Mature_143_residues
AKTAQFYILTENCTLTVEEIACNLAASIWRSGKKVLISCESEAQALEIDERLWQRDPNEFVPHNLSGEATQYPTPIEISW
LGKRNLQRRDLLINLQQEIPDFSHSFTQIIDFVPKDDALKTQARERYKQLRLQGWNLSTENVG

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity

COG id: COG2927

COG function: function code L; DNA polymerase III, chi subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790709, Length=142, Percent_Identity=50, Blast_Score=153, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HOLC_HAEIN (P43749)

Other databases:

- EMBL:   L42023
- EMBL:   L15391
- PIR:   I64121
- RefSeq:   NP_439550.1
- ProteinModelPortal:   P43749
- SMR:   P43749
- GeneID:   950308
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1397
- NMPDR:   fig|71421.1.peg.1333
- TIGR:   HI_1397
- HOGENOM:   HBG576341
- OMA:   NLADQVP
- ProtClustDB:   PRK05728
- BioCyc:   HINF71421:HI_1397-MONOMER
- BRENDA:   2.7.7.7
- InterPro:   IPR007459
- Gene3D:   G3DSA:3.40.50.10110

Pfam domain/function: PF04364 DNA_pol3_chi; SSF102400 DNA_pol3_chi

EC number: =2.7.7.7

Molecular weight: Translated: 16636; Mature: 16505

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTAQFYILTENCTLTVEEIACNLAASIWRSGKKVLISCESEAQALEIDERLWQRDPNE
CCCCEEEEEEECCCEEEHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCC
FVPHNLSGEATQYPTPIEISWLGKRNLQRRDLLINLQQEIPDFSHSFTQIIDFVPKDDAL
CCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHEEEHHHHCCCCHHHHHHHHHCCCCCCHH
KTQARERYKQLRLQGWNLSTENVG
HHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
AKTAQFYILTENCTLTVEEIACNLAASIWRSGKKVLISCESEAQALEIDERLWQRDPNE
CCCEEEEEEECCCEEEHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCC
FVPHNLSGEATQYPTPIEISWLGKRNLQRRDLLINLQQEIPDFSHSFTQIIDFVPKDDAL
CCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHEEEHHHHCCCCHHHHHHHHHCCCCCCHH
KTQARERYKQLRLQGWNLSTENVG
HHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 7959067