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The map label for this gene is hindIIIR [H]

Identifier: 16273303

GI number: 16273303

Start: 1491780

End: 1492682

Strand: Reverse

Name: hindIIIR [H]

Synonym: HI1393

Alternate gene names: 16273303

Gene position: 1492682-1491780 (Counterclockwise)

Preceding gene: 16273304

Following gene: 16273302

Centisome position: 81.56

GC content: 26.39

Gene sequence:

>903_bases
ATGAAGAAAAGTGCGTTAGAGAAATTATTATCACTGATAGAAAATTTAACTAATCAAGAATTTAAACAGGCAACTAATAG
TTTAATCTCATTTATTTATAAATTGAATAGAAATGAGGTTATTGAATTGGTTAGATCTATTGGAATACTTCCTGAGGCAA
TAAAACCAAGTTCTACACAAGAGAAATTATTCTCAAAAGCAGGAGATATTGTATTAGCTAAAGCTTTTCAATTACTTAAT
CTGAACTCAAAACCATTAGAACAGAGAGGTAATGCTGGAGATGTGATAGCACTCTCTAAAGAATTTAATTACGGCTTAGT
TGCTGATGCTAAATCTTTTCGACTTAGTCGTACTGCTAAAAATCAAAAAGATTTTAAAGTAAAAGCGTTAAGTGAATGGC
GAGAAGATAAGGATTATGCAGTATTAACAGCACCATTTTTTCAATATCCTACAACTAAAAGTCAAATTTTTAAGCAATCG
TTAGATGAAAATGTTTTGTTATTTAGTTGGGAACATTTAGCTATATTATTACAGTTAGATTTAGAGGAAACTAATATTTT
TYCTTTTGAGCAATTATGGAACTTTCCCAAAAAGCAAAGTAAAAAAACATCTGTATCTGATGCTGAAAATAATTTTATGA
GAGATTTTAATAAATACTTTATGGATTTATTTAAAATAGATAAAGATACTTTAAATCAACTTCTTCAAAAAGAGATCAAT
TTTATTGAAGAAAGATCCTTGATAGAAAAGGAATATTGGAAAAAACAAATAAATATTATAAAAAATTTTACAAGAGAAGA
AGCCATAGAAGCATTATTGAAAGATATAAATATGTCTTCAAAAATAGAAACTATTGATAGTTTTATTAAAGGGATAAAAA
GTAATGATAGACTGTATTTATAA

Upstream 100 bases:

>100_bases
GAGAATATGGGGTTGGATGAGTTGAAAGAAGTGATTCGGGGGATTTATCCGAATGAGATTATCTTTTGGGTAATTCAATT
TAGCTTAAAGGAGTATTTTA

Downstream 100 bases:

>100_bases
TTCTGATTCTATTTNTGAAATAAAAAAATTGGATAGTAATTCTATTCATGCAATTATCTCTGATATTCCTTATGGAATTG
ATTATGATGATTGGGATATA

Product: type II restriction endonuclease

Products: NA

Alternate protein names: R.HindIII; Endonuclease HindIII; Type II restriction enzyme HindIII [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MKKSALEKLLSLIENLTNQEFKQATNSLISFIYKLNRNEVIELVRSIGILPEAIKPSSTQEKLFSKAGDIVLAKAFQLLN
LNSKPLEQRGNAGDVIALSKEFNYGLVADAKSFRLSRTAKNQKDFKVKALSEWREDKDYAVLTAPFFQYPTTKSQIFKQS
LDENVLLFSWEHLAILLQLDLEETNIFXFEQLWNFPKKQSKKTSVSDAENNFMRDFNKYFMDLFKIDKDTLNQLLQKEIN
FIEERSLIEKEYWKKQINIIKNFTREEAIEALLKDINMSSKIETIDSFIKGIKSNDRLYL

Sequences:

>Translated_300_residues
MKKSALEKLLSLIENLTNQEFKQATNSLISFIYKLNRNEVIELVRSIGILPEAIKPSSTQEKLFSKAGDIVLAKAFQLLN
LNSKPLEQRGNAGDVIALSKEFNYGLVADAKSFRLSRTAKNQKDFKVKALSEWREDKDYAVLTAPFFQYPTTKSQIFKQS
LDENVLLFSWEHLAILLQLDLEETNIFXFEQLWNFPKKQSKKTSVSDAENNFMRDFNKYFMDLFKIDKDTLNQLLQKEIN
FIEERSLIEKEYWKKQINIIKNFTREEAIEALLKDINMSSKIETIDSFIKGIKSNDRLYL
>Mature_300_residues
MKKSALEKLLSLIENLTNQEFKQATNSLISFIYKLNRNEVIELVRSIGILPEAIKPSSTQEKLFSKAGDIVLAKAFQLLN
LNSKPLEQRGNAGDVIALSKEFNYGLVADAKSFRLSRTAKNQKDFKVKALSEWREDKDYAVLTAPFFQYPTTKSQIFKQS
LDENVLLFSWEHLAILLQLDLEETNIFXFEQLWNFPKKQSKKTSVSDAENNFMRDFNKYFMDLFKIDKDTLNQLLQKEIN
FIEERSLIEKEYWKKQINIIKNFTREEAIEALLKDINMSSKIETIDSFIKGIKSNDRLYL

Specific function: Recognizes the double-stranded sequence AAGCTT and cleaves after A-1 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019043 [H]

Pfam domain/function: PF09518 RE_HindIII [H]

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 34923; Mature: 34923

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSALEKLLSLIENLTNQEFKQATNSLISFIYKLNRNEVIELVRSIGILPEAIKPSSTQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCHH
EKLFSKAGDIVLAKAFQLLNLNSKPLEQRGNAGDVIALSKEFNYGLVADAKSFRLSRTAK
HHHHHHHCCHHHHHHHHHHCCCCCCHHHCCCCCCEEEEECCCCCCEEECHHHHHHHHHHC
NQKDFKVKALSEWREDKDYAVLTAPFFQYPTTKSQIFKQSLDENVLLFSWEHLAILLQLD
CCHHHHHHHHHHHCCCCCCEEEEECHHCCCCCHHHHHHHHCCCCEEEEEHHHHHEEHEEC
LEETNIFXFEQLWNFPKKQSKKTSVSDAENNFMRDFNKYFMDLFKIDKDTLNQLLQKEIN
CCHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
FIEERSLIEKEYWKKQINIIKNFTREEAIEALLKDINMSSKIETIDSFIKGIKSNDRLYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKKSALEKLLSLIENLTNQEFKQATNSLISFIYKLNRNEVIELVRSIGILPEAIKPSSTQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCHH
EKLFSKAGDIVLAKAFQLLNLNSKPLEQRGNAGDVIALSKEFNYGLVADAKSFRLSRTAK
HHHHHHHCCHHHHHHHHHHCCCCCCHHHCCCCCCEEEEECCCCCCEEECHHHHHHHHHHC
NQKDFKVKALSEWREDKDYAVLTAPFFQYPTTKSQIFKQSLDENVLLFSWEHLAILLQLD
CCHHHHHHHHHHHCCCCCCEEEEECHHCCCCCHHHHHHHHCCCCEEEEEHHHHHEEHEEC
LEETNIFXFEQLWNFPKKQSKKTSVSDAENNFMRDFNKYFMDLFKIDKDTLNQLLQKEIN
CCHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
FIEERSLIEKEYWKKQINIIKNFTREEAIEALLKDINMSSKIETIDSFIKGIKSNDRLYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7959067; 7542800 [H]