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The map label for this gene is hindIIIM [H]

Identifier: 16273302

GI number: 16273302

Start: 1490870

End: 1491799

Strand: Reverse

Name: hindIIIM [H]

Synonym: HI1392

Alternate gene names: 16273302

Gene position: 1491799-1490870 (Counterclockwise)

Preceding gene: 16273303

Following gene: 16273301

Centisome position: 81.51

GC content: 30.57

Gene sequence:

>930_bases
ATGATAGACTGTATTTATAATTCTGATTCTATTTNTGAAATAAAAAAATTGGATAGTAATTCTATTCATGCAATTATCTC
TGATATTCCTTATGGAATTGATTATGATGATTGGGATATATTGCATTCCAATACTAATAGTGCATTAGGAGGAACATCTT
CAGCTCAACACAAAACATCATTGTTTAAACGAAGAGGTAAACCATTAAATGGTTGGTCAGAGGCTGATAAGAAAAGACCG
CAAGAGTATCAAGAGTGGGTTGAAAGTTGGTCTAATGAATGGTTTAGAGTATTAAAGTCAGGAAGTTCAGTCTTTGTTTT
TGCAGGAAGACAATTTGCACACAGAGTTGTTGTTGCGTTTGAAAATAGTGGTTTTACTTTCAAAGATATGTTGAGTTGGG
AAAAAGACAAAGCTCCACATAGAGCACAAAGAATTTCTTGTGTGTTTGAACGTCGAGGTGATATTGCTAATACTAATAAA
TGGGTTGGTTGGAGAGTTGCTAATTTACGACCTTTATTTGAGCCTATTTTATGGTTTCAAAAACCATATAAAACTGGCAG
TACATTAGCTGATAATTTAATCAAACATGAAGTTGGAGCTTGGAATGAAAATTCGTTAACTCATTGGAATATACAACAAG
GAGCTTTAAATCATTCTAATATACTAAAAGTAAGAATAACTTCTGAGGATAAAGGTTATCATGTTGCTCAAAAACCACTT
AATCTAATGAAATTGCTTATTGATTTAGTAACAAAAGAAGAACAAATTGTATTAGATCCTTTTGCTGGTAGTGGTACTAC
ATTATTAGCAGCTAAAGAATTAAATAGACATTTCATTGGATATGAAAAAAATAATGGAATATATAATATTGCAGTTAATC
GTTTAGGGATAGAAAAAAATAACTGCTTTTATAATAAAGAGAAAAAATAA

Upstream 100 bases:

>100_bases
AATTTTACAAGAGAAGAAGCCATAGAAGCATTATTGAAAGATATAAATATGTCTTCAAAAATAGAAACTATTGATAGTTT
TATTAAAGGGATAAAAAGTA

Downstream 100 bases:

>100_bases
ATGACACAAAAATTCGAAATGGCAGACCGTTTTAATCCGTCTGCGGTAGAACAAGCCCTTTATCAACGTTGGGAAGAGAG
CGGTTATTTTAAACCGTCTG

Product: modification methylase

Products: NA

Alternate protein names: M.HindIII; Adenine-specific methyltransferase HindIII [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MIDCIYNSDSIXEIKKLDSNSIHAIISDIPYGIDYDDWDILHSNTNSALGGTSSAQHKTSLFKRRGKPLNGWSEADKKRP
QEYQEWVESWSNEWFRVLKSGSSVFVFAGRQFAHRVVVAFENSGFTFKDMLSWEKDKAPHRAQRISCVFERRGDIANTNK
WVGWRVANLRPLFEPILWFQKPYKTGSTLADNLIKHEVGAWNENSLTHWNIQQGALNHSNILKVRITSEDKGYHVAQKPL
NLMKLLIDLVTKEEQIVLDPFAGSGTTLLAAKELNRHFIGYEKNNGIYNIAVNRLGIEKNNCFYNKEKK

Sequences:

>Translated_309_residues
MIDCIYNSDSIXEIKKLDSNSIHAIISDIPYGIDYDDWDILHSNTNSALGGTSSAQHKTSLFKRRGKPLNGWSEADKKRP
QEYQEWVESWSNEWFRVLKSGSSVFVFAGRQFAHRVVVAFENSGFTFKDMLSWEKDKAPHRAQRISCVFERRGDIANTNK
WVGWRVANLRPLFEPILWFQKPYKTGSTLADNLIKHEVGAWNENSLTHWNIQQGALNHSNILKVRITSEDKGYHVAQKPL
NLMKLLIDLVTKEEQIVLDPFAGSGTTLLAAKELNRHFIGYEKNNGIYNIAVNRLGIEKNNCFYNKEKK
>Mature_309_residues
MIDCIYNSDSIXEIKKLDSNSIHAIISDIPYGIDYDDWDILHSNTNSALGGTSSAQHKTSLFKRRGKPLNGWSEADKKRP
QEYQEWVESWSNEWFRVLKSGSSVFVFAGRQFAHRVVVAFENSGFTFKDMLSWEKDKAPHRAQRISCVFERRGDIANTNK
WVGWRVANLRPLFEPILWFQKPYKTGSTLADNLIKHEVGAWNENSLTHWNIQQGALNHSNILKVRITSEDKGYHVAQKPL
NLMKLLIDLVTKEEQIVLDPFAGSGTTLLAAKELNRHFIGYEKNNGIYNIAVNRLGIEKNNCFYNKEKK

Specific function: This methylase recognizes the double-stranded sequence AAGCTT, causes specific methylation on A-1 on both strands, and protects the DNA from cleavage by the HindIII endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 35460; Mature: 35460

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDCIYNSDSIXEIKKLDSNSIHAIISDIPYGIDYDDWDILHSNTNSALGGTSSAQHKTS
CEEEEECCCCHHHHHHCCCCCCEEHHHHCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHH
LFKRRGKPLNGWSEADKKRPQEYQEWVESWSNEWFRVLKSGSSVFVFAGRQFAHRVVVAF
HHHHCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEEEE
ENSGFTFKDMLSWEKDKAPHRAQRISCVFERRGDIANTNKWVGWRVANLRPLFEPILWFQ
ECCCCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHC
KPYKTGSTLADNLIKHEVGAWNENSLTHWNIQQGALNHSNILKVRITSEDKGYHVAQKPL
CCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCCCHHHHHH
NLMKLLIDLVTKEEQIVLDPFAGSGTTLLAAKELNRHFIGYEKNNGIYNIAVNRLGIEKN
HHHHHHHHHHCCCCEEEEECCCCCCCEEEEHHHHHHHCCCEECCCCEEEEEEEEECCCCC
NCFYNKEKK
CCCCCCCCC
>Mature Secondary Structure
MIDCIYNSDSIXEIKKLDSNSIHAIISDIPYGIDYDDWDILHSNTNSALGGTSSAQHKTS
CEEEEECCCCHHHHHHCCCCCCEEHHHHCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHH
LFKRRGKPLNGWSEADKKRPQEYQEWVESWSNEWFRVLKSGSSVFVFAGRQFAHRVVVAF
HHHHCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEEEE
ENSGFTFKDMLSWEKDKAPHRAQRISCVFERRGDIANTNKWVGWRVANLRPLFEPILWFQ
ECCCCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHC
KPYKTGSTLADNLIKHEVGAWNENSLTHWNIQQGALNHSNILKVRITSEDKGYHVAQKPL
CCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCCCHHHHHH
NLMKLLIDLVTKEEQIVLDPFAGSGTTLLAAKELNRHFIGYEKNNGIYNIAVNRLGIEKN
HHHHHHHHHHCCCCEEEEECCCCCCCEEEEHHHHHHHCCCEECCCCEEEEEEEEECCCCC
NCFYNKEKK
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7959067; 7542800 [H]