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The map label for this gene is thrS [H]

Identifier: 16273277

GI number: 16273277

Start: 1454489

End: 1456420

Strand: Direct

Name: thrS [H]

Synonym: HI1367

Alternate gene names: 16273277

Gene position: 1454489-1456420 (Clockwise)

Preceding gene: 16273275

Following gene: 30995445

Centisome position: 79.47

GC content: 40.53

Gene sequence:

>1932_bases
ATGCCAATTATTACTTTACCTGACGGTTCCCAACGTCAATTCGATCGTCCTGTTTCTGTTCTCGAAGTCGCACAAGATAT
TGGTGCGGGTCTTGCTAAAGCCACTATCGCAGGCCGTGTAAACGGAGAACGTCGCGATGCGTGCTATGTTATCGAGCAAG
ATGCAACGTTAGAAATTATTACTGCAAAAGATGAAGACGGTTTAGAGATTATCCGCCATTCTTGCGCTCACTTGCTCGGA
CACGCTATTAAACAATTATTCCCAGATGTAAAAATGGCAATTGGTCCAACTATTGAAAACGGCTTCTATTATGATGTAGA
TTTAGATCGCTCTTTAACCCAAGAAGATATTGATGCAATCGAAAAACGTATGTTGGAATTGGCAAAAACAAATTATGACG
TCGTGAAAAAACGTGTTACTTGGCAAGAAGCGCGTGATACCTTTGAAAAACGCGGCGAGCCATACAAAATGGCAATTTTA
GATGAAAACATTGAACGTACTGCAACGCCTGCGTTATATCATCACTTAGAATATATTGATATGTGCCGTGGACCACACGT
GCCAAATATGCGTTTCTGTCAGCATTTCAAATTACAAAAAGTTGCTGGTGCGTACTGGCGTGGCGACAGTAAAAACAAAA
TGTTACAACGTATCTATGGTACAGCTTGGGCGGATAAAAAACAACTTGCTGAATATTTAACACGCTTAGAAGAAGCGGCA
AAACGTGACCACCGTAAAATTGGTAAAGCATTAGATTTATATCATATGCAAGAAGAAGCACCAGGTATGGTGTTCTGGCA
CAATGATGGCTGGACAATTTTCCGTGAGCTTGAAACGTTCGTGCGTACTAAATTAAAACAATACGATTATCAAGAAGTGA
AAGGCCCATTTATGATGGATCGCGTATTGTGGGAAAAAACAGGTCACTGGCAAAATTACGCAGATTTAATGTTTACCACT
CAATCTGAAAACCGTGAATACGCAATTAAACCGATGAACTGCCCAGGCCACGTTCAAATTTTTAATCAAGGTTTAAAATC
TTACCGTGACTTACCAATCCGTATGGCGGAATTTGGTTCTTGCCATCGTAATGAACCATCAGGTTCTTTACACGGTTTAA
TGCGTGTTCGTGGCTTTACTCAAGATGATGCTCACATTTTCTGTACTGAAGATCAAATTGAGAGCGAAGTAACTAGCTGT
ATCAAAATGGTGTACGACATTTATAGCACCTTTGGTTTCACTAATATCGCAGTAAAACTTTCTACTCGCCCTGAAAATCG
TATCGGTTCAGATGAAATGTGGGATCGTGCGGAAGCAGGTCTTGCTGCAGCCTTAGCACACAACGGTCTTGAATATGAAA
TTCAAGAAGGTGAAGGTGCATTCTATGGTCCAAAAATTGAGTTTGCACTACGAGATTGTTTAGGTCGTGAATGGCAATGT
GGTACCGTGCAATTAGACTTCGCATTACCAGGTCGTTTAGATGCAACTTATGTAGCAGAAGATAATAGCCGTAAAACCCC
TGTTATGATTCACCGTGCGATTTTAGGTTCTATTGAGCGTTTCATTGGTATTATTACTGAAGAATATGCAGGTTTCTTCC
CTGCATGGTTAGCACCAACCCAAGCAGTTGTAATGAATATCACTGATAGCCAAGCAGACTATGTACAGAAAGTCGCAAAA
CAGCTTTCTGATGTAGGTTTACGCGTGAAAACTGATTTACGTAACGAAAAAGTGGGCTTCAAAATTCGTGAACATACTTT
ACGCCGTGTGCCTTATATGCTCGTTTGTGGCGATAAAGAAATCGCAGAAGGCAAAGTGGCTGTACGTACCCGTAAAGGTG
CTGATTTAGGCACATTTACTGTGGAAGAATTTGCGGAAATCTTAAAAAATCAAGTAAGAAGCCGTGAATTAAAGTTATTG
AATGAAGAATAA

Upstream 100 bases:

>100_bases
GGAATGAAGCCAAGTAGGGCAAAAGTGCGGTTAATTTTTATAGAATTTTAAATGAACACGTCACCTTTCGTATGGGTGAC
CACTGTATAAGGAAAAAACA

Downstream 100 bases:

>100_bases
TACTTCTAGAAAATAACCAATATAATTTACTGTTTAAATAGTGAGTTCAAACGGTGATCGTTTAAGCGATCACCGCCCTT
TTTATCTATTTCATATCTAT

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 643; Mature: 642

Protein sequence:

>643_residues
MPIITLPDGSQRQFDRPVSVLEVAQDIGAGLAKATIAGRVNGERRDACYVIEQDATLEIITAKDEDGLEIIRHSCAHLLG
HAIKQLFPDVKMAIGPTIENGFYYDVDLDRSLTQEDIDAIEKRMLELAKTNYDVVKKRVTWQEARDTFEKRGEPYKMAIL
DENIERTATPALYHHLEYIDMCRGPHVPNMRFCQHFKLQKVAGAYWRGDSKNKMLQRIYGTAWADKKQLAEYLTRLEEAA
KRDHRKIGKALDLYHMQEEAPGMVFWHNDGWTIFRELETFVRTKLKQYDYQEVKGPFMMDRVLWEKTGHWQNYADLMFTT
QSENREYAIKPMNCPGHVQIFNQGLKSYRDLPIRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEDQIESEVTSC
IKMVYDIYSTFGFTNIAVKLSTRPENRIGSDEMWDRAEAGLAAALAHNGLEYEIQEGEGAFYGPKIEFALRDCLGREWQC
GTVQLDFALPGRLDATYVAEDNSRKTPVMIHRAILGSIERFIGIITEEYAGFFPAWLAPTQAVVMNITDSQADYVQKVAK
QLSDVGLRVKTDLRNEKVGFKIREHTLRRVPYMLVCGDKEIAEGKVAVRTRKGADLGTFTVEEFAEILKNQVRSRELKLL
NEE

Sequences:

>Translated_643_residues
MPIITLPDGSQRQFDRPVSVLEVAQDIGAGLAKATIAGRVNGERRDACYVIEQDATLEIITAKDEDGLEIIRHSCAHLLG
HAIKQLFPDVKMAIGPTIENGFYYDVDLDRSLTQEDIDAIEKRMLELAKTNYDVVKKRVTWQEARDTFEKRGEPYKMAIL
DENIERTATPALYHHLEYIDMCRGPHVPNMRFCQHFKLQKVAGAYWRGDSKNKMLQRIYGTAWADKKQLAEYLTRLEEAA
KRDHRKIGKALDLYHMQEEAPGMVFWHNDGWTIFRELETFVRTKLKQYDYQEVKGPFMMDRVLWEKTGHWQNYADLMFTT
QSENREYAIKPMNCPGHVQIFNQGLKSYRDLPIRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEDQIESEVTSC
IKMVYDIYSTFGFTNIAVKLSTRPENRIGSDEMWDRAEAGLAAALAHNGLEYEIQEGEGAFYGPKIEFALRDCLGREWQC
GTVQLDFALPGRLDATYVAEDNSRKTPVMIHRAILGSIERFIGIITEEYAGFFPAWLAPTQAVVMNITDSQADYVQKVAK
QLSDVGLRVKTDLRNEKVGFKIREHTLRRVPYMLVCGDKEIAEGKVAVRTRKGADLGTFTVEEFAEILKNQVRSRELKLL
NEE
>Mature_642_residues
PIITLPDGSQRQFDRPVSVLEVAQDIGAGLAKATIAGRVNGERRDACYVIEQDATLEIITAKDEDGLEIIRHSCAHLLGH
AIKQLFPDVKMAIGPTIENGFYYDVDLDRSLTQEDIDAIEKRMLELAKTNYDVVKKRVTWQEARDTFEKRGEPYKMAILD
ENIERTATPALYHHLEYIDMCRGPHVPNMRFCQHFKLQKVAGAYWRGDSKNKMLQRIYGTAWADKKQLAEYLTRLEEAAK
RDHRKIGKALDLYHMQEEAPGMVFWHNDGWTIFRELETFVRTKLKQYDYQEVKGPFMMDRVLWEKTGHWQNYADLMFTTQ
SENREYAIKPMNCPGHVQIFNQGLKSYRDLPIRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEDQIESEVTSCI
KMVYDIYSTFGFTNIAVKLSTRPENRIGSDEMWDRAEAGLAAALAHNGLEYEIQEGEGAFYGPKIEFALRDCLGREWQCG
TVQLDFALPGRLDATYVAEDNSRKTPVMIHRAILGSIERFIGIITEEYAGFFPAWLAPTQAVVMNITDSQADYVQKVAKQ
LSDVGLRVKTDLRNEKVGFKIREHTLRRVPYMLVCGDKEIAEGKVAVRTRKGADLGTFTVEEFAEILKNQVRSRELKLLN
EE

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38570111, Length=619, Percent_Identity=40.3877221324717, Blast_Score=473, Evalue=1e-133,
Organism=Homo sapiens, GI38202255, Length=642, Percent_Identity=39.7196261682243, Blast_Score=471, Evalue=1e-132,
Organism=Homo sapiens, GI20070344, Length=627, Percent_Identity=34.7687400318979, Blast_Score=389, Evalue=1e-108,
Organism=Escherichia coli, GI1788013, Length=642, Percent_Identity=78.0373831775701, Blast_Score=1080, Evalue=0.0,
Organism=Escherichia coli, GI1786392, Length=184, Percent_Identity=26.0869565217391, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71984184, Length=628, Percent_Identity=38.8535031847134, Blast_Score=463, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI71984192, Length=628, Percent_Identity=38.8535031847134, Blast_Score=463, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6322112, Length=650, Percent_Identity=36.6153846153846, Blast_Score=435, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6322654, Length=419, Percent_Identity=38.1861575178998, Blast_Score=304, Evalue=3e-83,
Organism=Drosophila melanogaster, GI24583839, Length=646, Percent_Identity=39.938080495356, Blast_Score=483, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24583842, Length=646, Percent_Identity=39.938080495356, Blast_Score=483, Evalue=1e-136,
Organism=Drosophila melanogaster, GI28574141, Length=646, Percent_Identity=39.938080495356, Blast_Score=483, Evalue=1e-136,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 73713; Mature: 73582

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIITLPDGSQRQFDRPVSVLEVAQDIGAGLAKATIAGRVNGERRDACYVIEQDATLEII
CCEEECCCCCCHHHCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEEEECCCEEEEE
TAKDEDGLEIIRHSCAHLLGHAIKQLFPDVKMAIGPTIENGFYYDVDLDRSLTQEDIDAI
ECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCEECCCEEEEEECCCCCCHHHHHHH
EKRMLELAKTNYDVVKKRVTWQEARDTFEKRGEPYKMAILDENIERTATPALYHHLEYID
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCHHHHHHHHHHH
MCRGPHVPNMRFCQHFKLQKVAGAYWRGDSKNKMLQRIYGTAWADKKQLAEYLTRLEEAA
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
KRDHRKIGKALDLYHMQEEAPGMVFWHNDGWTIFRELETFVRTKLKQYDYQEVKGPFMMD
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHCCCHHHH
RVLWEKTGHWQNYADLMFTTQSENREYAIKPMNCPGHVQIFNQGLKSYRDLPIRMAEFGS
HHHHHHCCCCCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEHHHHCC
CHRNEPSGSLHGLMRVRGFTQDDAHIFCTEDQIESEVTSCIKMVYDIYSTFGFTNIAVKL
CCCCCCCHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEE
STRPENRIGSDEMWDRAEAGLAAALAHNGLEYEIQEGEGAFYGPKIEFALRDCLGREWQC
ECCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEECCCHHHHHHHHCCCCCCC
GTVQLDFALPGRLDATYVAEDNSRKTPVMIHRAILGSIERFIGIITEEYAGFFPAWLAPT
CEEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCC
QAVVMNITDSQADYVQKVAKQLSDVGLRVKTDLRNEKVGFKIREHTLRRVPYMLVCGDKE
EEEEEEECCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHCCEEEEECCCH
IAEGKVAVRTRKGADLGTFTVEEFAEILKNQVRSRELKLLNEE
HCCCEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PIITLPDGSQRQFDRPVSVLEVAQDIGAGLAKATIAGRVNGERRDACYVIEQDATLEII
CEEECCCCCCHHHCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEEEECCCEEEEE
TAKDEDGLEIIRHSCAHLLGHAIKQLFPDVKMAIGPTIENGFYYDVDLDRSLTQEDIDAI
ECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCEECCCEEEEEECCCCCCHHHHHHH
EKRMLELAKTNYDVVKKRVTWQEARDTFEKRGEPYKMAILDENIERTATPALYHHLEYID
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCHHHHHHHHHHH
MCRGPHVPNMRFCQHFKLQKVAGAYWRGDSKNKMLQRIYGTAWADKKQLAEYLTRLEEAA
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
KRDHRKIGKALDLYHMQEEAPGMVFWHNDGWTIFRELETFVRTKLKQYDYQEVKGPFMMD
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHCCCHHHH
RVLWEKTGHWQNYADLMFTTQSENREYAIKPMNCPGHVQIFNQGLKSYRDLPIRMAEFGS
HHHHHHCCCCCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEHHHHCC
CHRNEPSGSLHGLMRVRGFTQDDAHIFCTEDQIESEVTSCIKMVYDIYSTFGFTNIAVKL
CCCCCCCHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEE
STRPENRIGSDEMWDRAEAGLAAALAHNGLEYEIQEGEGAFYGPKIEFALRDCLGREWQC
ECCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEECCCHHHHHHHHCCCCCCC
GTVQLDFALPGRLDATYVAEDNSRKTPVMIHRAILGSIERFIGIITEEYAGFFPAWLAPT
CEEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCC
QAVVMNITDSQADYVQKVAKQLSDVGLRVKTDLRNEKVGFKIREHTLRRVPYMLVCGDKE
EEEEEEECCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHCCEEEEECCCH
IAEGKVAVRTRKGADLGTFTVEEFAEILKNQVRSRELKLLNEE
HCCCEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA