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The map label for this gene is cysB

Identifier: 16273120

GI number: 16273120

Start: 1266047

End: 1267018

Strand: Direct

Name: cysB

Synonym: HI1200

Alternate gene names: 16273120

Gene position: 1266047-1267018 (Clockwise)

Preceding gene: 16273119

Following gene: 30995431

Centisome position: 69.18

GC content: 39.2

Gene sequence:

>972_bases
ATGAAAATGCATCAATTGCGTTATATCGTTGAGATTGTAAACCAAAATTTGAATGTCACAGAAGCTGCAAATGCACTTTA
CACATCACAGCCGGGTATCAGTAAACAAGTTCGCCTATTAGAAGATGAATTAGGTCTGGAAATTTTTGAGCGTCATGGCA
AGCATATAAAATCAATTACACCAGCAGGGAAAAAGATTATCTCAATTGCACGCGAGTTACTTGTTAAAGCGCAAGGTATC
CGTGCCGTTGCCGATGAATATACTCGGCCAAATCACGGTGTATTACGTATTGCGACGACAAATACTCAGGCTCGTTATAT
GCTGCCAAGCGTGATTGAACGTTTTTCTAAAAAATATCCTGATGTGAGCTTGCATATTCATCAAGGTTCGCCAACACAAA
TTCACGATGCTTTAATGTCAGGTGAAGTGGATTTGGCAATTACCACTGAAGCACCTTATTTATTTGATGATTTAGTTCAG
ATTCCATGCTATTGGTGGAATCGTGCCGTGATTGTTTCGCCAGAACATCCGCTTGCCAAAGTGAAAGAACTGACCATTGA
GGAATTAGGCAAATACCCACTTGTGACTTACACGTTTGGTTTTACGGGTGTTTCTGATTTAGATTACGCATTTAATAGTG
CAGGCATTTTACCAAATATAGTTTTTACGGCTACCGATGCTGATGTTATTAAAACTTATGTGCGCTTGGGTTTAGGGGTG
GGCATTATGGCATCTATGGCACATACGGCATTAGATACGGATCTTGTGCTAATTGATGCAAGCCATTTGTTCCGCCCGAG
TATGACGAATATTGCATTTAAACATAGCACGTTCTTACGTAATTATATGTACGATTTCATGGAATATTTTTCACCGCACT
TAACTCGTAGTGTCGTAGAAAAAGCCGAGCGTTTGCGAGATAACAATTCAGTGAAAAAATTATTTGAAGGCATTGAATTG
GAAACAAAATAA

Upstream 100 bases:

>100_bases
GTAAAACAGGCTAGTCGTCGTCCAAAATCTGGGCAAATTCGTAAAGCGGTAAAACGTTATTCGGAGATGAACAAACGTTA
TAAAAAATAAGGACTTTTTT

Downstream 100 bases:

>100_bases
CAATGTCCAAATGAAGTGCGGTTGAAATTTAATAAGAATTTTAATCGCACTTAAAAGATTAAAACAGATCGTGATATTTC
ACTAAATCCTTACGCTGAAT

Product: transcriptional regulator CysB

Products: NA

Alternate protein names: Cys regulon transcriptional activator

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MKMHQLRYIVEIVNQNLNVTEAANALYTSQPGISKQVRLLEDELGLEIFERHGKHIKSITPAGKKIISIARELLVKAQGI
RAVADEYTRPNHGVLRIATTNTQARYMLPSVIERFSKKYPDVSLHIHQGSPTQIHDALMSGEVDLAITTEAPYLFDDLVQ
IPCYWWNRAVIVSPEHPLAKVKELTIEELGKYPLVTYTFGFTGVSDLDYAFNSAGILPNIVFTATDADVIKTYVRLGLGV
GIMASMAHTALDTDLVLIDASHLFRPSMTNIAFKHSTFLRNYMYDFMEYFSPHLTRSVVEKAERLRDNNSVKKLFEGIEL
ETK

Sequences:

>Translated_323_residues
MKMHQLRYIVEIVNQNLNVTEAANALYTSQPGISKQVRLLEDELGLEIFERHGKHIKSITPAGKKIISIARELLVKAQGI
RAVADEYTRPNHGVLRIATTNTQARYMLPSVIERFSKKYPDVSLHIHQGSPTQIHDALMSGEVDLAITTEAPYLFDDLVQ
IPCYWWNRAVIVSPEHPLAKVKELTIEELGKYPLVTYTFGFTGVSDLDYAFNSAGILPNIVFTATDADVIKTYVRLGLGV
GIMASMAHTALDTDLVLIDASHLFRPSMTNIAFKHSTFLRNYMYDFMEYFSPHLTRSVVEKAERLRDNNSVKKLFEGIEL
ETK
>Mature_323_residues
MKMHQLRYIVEIVNQNLNVTEAANALYTSQPGISKQVRLLEDELGLEIFERHGKHIKSITPAGKKIISIARELLVKAQGI
RAVADEYTRPNHGVLRIATTNTQARYMLPSVIERFSKKYPDVSLHIHQGSPTQIHDALMSGEVDLAITTEAPYLFDDLVQ
IPCYWWNRAVIVSPEHPLAKVKELTIEELGKYPLVTYTFGFTGVSDLDYAFNSAGILPNIVFTATDADVIKTYVRLGLGV
GIMASMAHTALDTDLVLIDASHLFRPSMTNIAFKHSTFLRNYMYDFMEYFSPHLTRSVVEKAERLRDNNSVKKLFEGIEL
ETK

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1787530, Length=324, Percent_Identity=63.2716049382716, Blast_Score=428, Evalue=1e-121,
Organism=Escherichia coli, GI1788296, Length=309, Percent_Identity=41.747572815534, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI157672245, Length=226, Percent_Identity=32.3008849557522, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1790399, Length=251, Percent_Identity=25.0996015936255, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788887, Length=253, Percent_Identity=28.4584980237154, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788481, Length=244, Percent_Identity=25.8196721311475, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787601, Length=187, Percent_Identity=30.4812834224599, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1790262, Length=204, Percent_Identity=25.9803921568627, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI2367136, Length=242, Percent_Identity=26.0330578512397, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787879, Length=253, Percent_Identity=26.0869565217391, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI145693105, Length=188, Percent_Identity=25, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789204, Length=239, Percent_Identity=25.5230125523013, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788626, Length=215, Percent_Identity=27.4418604651163, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081904, Length=220, Percent_Identity=26.3636363636364, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSB_HAEIN (P45105)

Other databases:

- EMBL:   L42023
- PIR:   E64189
- RefSeq:   NP_439356.1
- ProteinModelPortal:   P45105
- SMR:   P45105
- GeneID:   950152
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1200
- TIGR:   HI_1200
- HOGENOM:   HBG289996
- OMA:   AVADEYT
- ProtClustDB:   PRK12681
- BioCyc:   HINF71421:HI_1200-MONOMER
- GO:   GO:0005737
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00039

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 36486; Mature: 36486

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMHQLRYIVEIVNQNLNVTEAANALYTSQPGISKQVRLLEDELGLEIFERHGKHIKSIT
CCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCC
PAGKKIISIARELLVKAQGIRAVADEYTRPNHGVLRIATTNTQARYMLPSVIERFSKKYP
CCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCC
DVSLHIHQGSPTQIHDALMSGEVDLAITTEAPYLFDDLVQIPCYWWNRAVIVSPEHPLAK
CEEEEEECCCCHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCEECCCEEEECCCCCHHH
VKELTIEELGKYPLVTYTFGFTGVSDLDYAFNSAGILPNIVFTATDADVIKTYVRLGLGV
HHHHHHHHHCCCCEEEEEECCCCCCHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHCCH
GIMASMAHTALDTDLVLIDASHLFRPSMTNIAFKHSTFLRNYMYDFMEYFSPHLTRSVVE
HHHHHHHHHHHCCCEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
KAERLRDNNSVKKLFEGIELETK
HHHHHCCCCHHHHHHCCCCCCCC
>Mature Secondary Structure
MKMHQLRYIVEIVNQNLNVTEAANALYTSQPGISKQVRLLEDELGLEIFERHGKHIKSIT
CCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCC
PAGKKIISIARELLVKAQGIRAVADEYTRPNHGVLRIATTNTQARYMLPSVIERFSKKYP
CCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCC
DVSLHIHQGSPTQIHDALMSGEVDLAITTEAPYLFDDLVQIPCYWWNRAVIVSPEHPLAK
CEEEEEECCCCHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCEECCCEEEECCCCCHHH
VKELTIEELGKYPLVTYTFGFTGVSDLDYAFNSAGILPNIVFTATDADVIKTYVRLGLGV
HHHHHHHHHCCCCEEEEEECCCCCCHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHCCH
GIMASMAHTALDTDLVLIDASHLFRPSMTNIAFKHSTFLRNYMYDFMEYFSPHLTRSVVE
HHHHHHHHHHHCCCEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
KAERLRDNNSVKKLFEGIELETK
HHHHHCCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800