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The map label for this gene is rluB

Identifier: 16273119

GI number: 16273119

Start: 1264963

End: 1266036

Strand: Direct

Name: rluB

Synonym: HI1199

Alternate gene names: 16273119

Gene position: 1264963-1266036 (Clockwise)

Preceding gene: 16273118

Following gene: 16273120

Centisome position: 69.12

GC content: 42.09

Gene sequence:

>1074_bases
ATGAAACCTAGTCAAAAACAAACTCAAAGACAACCGCACTTTTCCAAAGATTCAGCAAAGAAACGTGATTTTTCAGCTAA
AAATGACAGACGTTCAGTATCTCGTACTGCTCGCATAGAAACAGCAAATACGAAAAAAAGTGCGGTTAATTCTGACAACA
AATTTTTATCTAAGCCAAAAGCGAAACCTGTTGTAAGAGCCTCGAATCAACCGAAAGCAGAGGGCGAAAAATTACAGAAA
GTTTTAGCGCGTGCTGGACAAGGCTCTCGTCGTGAAATTGAAACGATGATTGCCGCAGGCAGAGTGAGCGTAGAGGGTAA
AATTGCGACTTTGGGCGATCGTATTGACGTACATTCTGGCGTGAAAGTGCGTATTGATGGTCAAATCATTAATCTTAGCC
ACACACAAAAAGAAATTTGCCGTGTATTAATGTATTACAAACCTGAAGGCGAACTTTGTACACGTAGCGACCCAGAAGGG
CGCGCGACCGTGTTTGATCGTTTGCCGCGTTTAACAGGCTCTCGTTGGATTGCGGTGGGGCGTTTAGATATTAATACATC
GGGTTTATTGCTTTTTACCACTGATGGCGAATTAGCAAATCGTTTAATGCACCCAAGCCGTGAAGTTGAACGTGAATATT
CTGTGCGTGTATTTGGTCAAGTTGATGATGCGATGTTAGCACGTTTACGTAAAGGCGTTCAGCTTGAAGATGGCCTTGCC
AACTTTAAAGAAATTAAATTTACAGGCGGTGTGGGTATTAACCAATGGTATGACGTAACCTTGATGGAGGGACGTAACCG
TGAGGTTCGTCGTTTGTGGGAATCCCAAGGGATTCAAGTTAGCCGCTTGATTCGTATTCGTTATGGAAATATCAAACTGA
TGAAAGGTTTGCCTCGTGGTGGCTGGGAAGAAATGGATCTTGAAAACGTCAATTATTTACGTGAATTGGTGGGATTACCT
GCTGAGACTGAAACGAAATTAGATGTAAAACAGGCTAGTCGTCGTCCAAAATCTGGGCAAATTCGTAAAGCGGTAAAACG
TTATTCGGAGATGAACAAACGTTATAAAAAATAA

Upstream 100 bases:

>100_bases
AATCGCCAGTGATTTTACGCGAAGGAAGTGGAAGTACTGCTCCTTTTATTTAACTTATAGACGCTTGTGAAAGCGACCTT
TCCATTATTCAAGGAAAACA

Downstream 100 bases:

>100_bases
GGACTTTTTTATGAAAATGCATCAATTGCGTTATATCGTTGAGATTGTAAACCAAAATTTGAATGTCACAGAAGCTGCAA
ATGCACTTTACACATCACAG

Product: hypothetical protein

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MKPSQKQTQRQPHFSKDSAKKRDFSAKNDRRSVSRTARIETANTKKSAVNSDNKFLSKPKAKPVVRASNQPKAEGEKLQK
VLARAGQGSRREIETMIAAGRVSVEGKIATLGDRIDVHSGVKVRIDGQIINLSHTQKEICRVLMYYKPEGELCTRSDPEG
RATVFDRLPRLTGSRWIAVGRLDINTSGLLLFTTDGELANRLMHPSREVEREYSVRVFGQVDDAMLARLRKGVQLEDGLA
NFKEIKFTGGVGINQWYDVTLMEGRNREVRRLWESQGIQVSRLIRIRYGNIKLMKGLPRGGWEEMDLENVNYLRELVGLP
AETETKLDVKQASRRPKSGQIRKAVKRYSEMNKRYKK

Sequences:

>Translated_357_residues
MKPSQKQTQRQPHFSKDSAKKRDFSAKNDRRSVSRTARIETANTKKSAVNSDNKFLSKPKAKPVVRASNQPKAEGEKLQK
VLARAGQGSRREIETMIAAGRVSVEGKIATLGDRIDVHSGVKVRIDGQIINLSHTQKEICRVLMYYKPEGELCTRSDPEG
RATVFDRLPRLTGSRWIAVGRLDINTSGLLLFTTDGELANRLMHPSREVEREYSVRVFGQVDDAMLARLRKGVQLEDGLA
NFKEIKFTGGVGINQWYDVTLMEGRNREVRRLWESQGIQVSRLIRIRYGNIKLMKGLPRGGWEEMDLENVNYLRELVGLP
AETETKLDVKQASRRPKSGQIRKAVKRYSEMNKRYKK
>Mature_357_residues
MKPSQKQTQRQPHFSKDSAKKRDFSAKNDRRSVSRTARIETANTKKSAVNSDNKFLSKPKAKPVVRASNQPKAEGEKLQK
VLARAGQGSRREIETMIAAGRVSVEGKIATLGDRIDVHSGVKVRIDGQIINLSHTQKEICRVLMYYKPEGELCTRSDPEG
RATVFDRLPRLTGSRWIAVGRLDINTSGLLLFTTDGELANRLMHPSREVEREYSVRVFGQVDDAMLARLRKGVQLEDGLA
NFKEIKFTGGVGINQWYDVTLMEGRNREVRRLWESQGIQVSRLIRIRYGNIKLMKGLPRGGWEEMDLENVNYLRELVGLP
AETETKLDVKQASRRPKSGQIRKAVKRYSEMNKRYKK

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Escherichia coli, GI1787524, Length=277, Percent_Identity=73.6462093862816, Blast_Score=426, Evalue=1e-120,
Organism=Escherichia coli, GI1790453, Length=245, Percent_Identity=29.3877551020408, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI1788510, Length=236, Percent_Identity=30.0847457627119, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI87081838, Length=185, Percent_Identity=32.972972972973, Blast_Score=78, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLUB_HAEIN (P45104)

Other databases:

- EMBL:   L42023
- PIR:   A64169
- RefSeq:   NP_439355.1
- ProteinModelPortal:   P45104
- SMR:   P45104
- GeneID:   950150
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1199
- NMPDR:   fig|71421.1.peg.1146
- TIGR:   HI_1199
- HOGENOM:   HBG754478
- OMA:   ELCTRSD
- ProtClustDB:   CLSK870190
- BioCyc:   HINF71421:HI_1199-MONOMER
- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942
- SMART:   SM00363
- TIGRFAMs:   TIGR00093

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4; SSF55120 PsdUridine_synth_cat_dom

EC number: NA

Molecular weight: Translated: 40637; Mature: 40637

Theoretical pI: Translated: 10.91; Mature: 10.91

Prosite motif: PS01149 PSI_RSU; PS50889 S4

Important sites: ACT_SITE 183-183

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSQKQTQRQPHFSKDSAKKRDFSAKNDRRSVSRTARIETANTKKSAVNSDNKFLSKPK
CCCCHHHHHCCCCCCCCCHHHCCCCCCCHHHHHHHHHEEEECCCHHHHCCCCCHHHCCCC
AKPVVRASNQPKAEGEKLQKVLARAGQGSRREIETMIAAGRVSVEGKIATLGDRIDVHSG
CCCEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEECCEEEECCCEEECCCC
VKVRIDGQIINLSHTQKEICRVLMYYKPEGELCTRSDPEGRATVFDRLPRLTGSRWIAVG
CEEEECCEEEECCHHHHHHHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEE
RLDINTSGLLLFTTDGELANRLMHPSREVEREYSVRVFGQVDDAMLARLRKGVQLEDGLA
EEEECCCCEEEEECCHHHHHHHCCCCHHHHHHCCEEEECCCCHHHHHHHHCCCCCHHHHC
NFKEIKFTGGVGINQWYDVTLMEGRNREVRRLWESQGIQVSRLIRIRYGNIKLMKGLPRG
CHHEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEEEEEEECCEEEEECCCCC
GWEEMDLENVNYLRELVGLPAETETKLDVKQASRRPKSGQIRKAVKRYSEMNKRYKK
CCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKPSQKQTQRQPHFSKDSAKKRDFSAKNDRRSVSRTARIETANTKKSAVNSDNKFLSKPK
CCCCHHHHHCCCCCCCCCHHHCCCCCCCHHHHHHHHHEEEECCCHHHHCCCCCHHHCCCC
AKPVVRASNQPKAEGEKLQKVLARAGQGSRREIETMIAAGRVSVEGKIATLGDRIDVHSG
CCCEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEECCEEEECCCEEECCCC
VKVRIDGQIINLSHTQKEICRVLMYYKPEGELCTRSDPEGRATVFDRLPRLTGSRWIAVG
CEEEECCEEEECCHHHHHHHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEE
RLDINTSGLLLFTTDGELANRLMHPSREVEREYSVRVFGQVDDAMLARLRKGVQLEDGLA
EEEECCCCEEEEECCHHHHHHHCCCCHHHHHHCCEEEECCCCHHHHHHHHCCCCCHHHHC
NFKEIKFTGGVGINQWYDVTLMEGRNREVRRLWESQGIQVSRLIRIRYGNIKLMKGLPRG
CHHEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEEEEEEECCEEEEECCCCC
GWEEMDLENVNYLRELVGLPAETETKLDVKQASRRPKSGQIRKAVKRYSEMNKRYKK
CCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023