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The map label for this gene is metX [H]

Identifier: 16273096

GI number: 16273096

Start: 1242025

End: 1243179

Strand: Direct

Name: metX [H]

Synonym: HI1172

Alternate gene names: 16273096

Gene position: 1242025-1243179 (Clockwise)

Preceding gene: 16273095

Following gene: 16273097

Centisome position: 67.87

GC content: 39.57

Gene sequence:

>1155_bases
ATGTCTAGTTATTTATTTACATCTGAATCAGTCTCTGAAGGGCATCCAGATAAAATTGCCGATCAAATTTCTGATGCGGT
ACTTGATGAAATCCTAAAACAAGATCCAAAAGCACGAGTAGCTTGTGAAACTTACGTAAAAACTGGTATGGCATTGGTTG
GTGGTGAAATTACAACATCAGCATGGGTTGATATTGAGAATTTAACCCGTAAAGTGATTTGTGATATTGGTTATGAACAT
TCTGAAATGGGCTTTGATGGTCATTCTTGTGCAGTGCTTAATGCGATTGGTAAACAATCTGCAGATATTAATCAAGGCGT
TGATCGTGAAAATCCATTAGATCAAGGCGCAGGCGACCAAGGTATTATGTTTGGTTATGCCACCAATGAAACGGATGTAT
TAATGCCTGCAGCCATTACTTATGCACATCGTTTAATGGAAAAACAATCTGAAGTGCGTAAAAGCGGTAAATTAGCATGG
TTACGCCCAGATGCGAAAAGCCAAGTTACTTTAAAATATGAAGATAATAAAATTGTTGGTGTGGATGCGGTTGTGCTTTC
TACTCAACATTCTGAAGAAGTTAGCCAAAAAGATTTACATGAAGGTGTGATGGAAGAAATTATCAAACCTGTGTTACCAA
GTGAATGGCTTTCTAAAGAAACAAAATTCTTCATTAACCCGACTGGTCGTTTTGTTATCGGTGGGCCAATGGGCGATTGT
GGTTTAACTGGCCGTAAAATCATCGTGGATACTTACGGTGGTGCCGCTCGTCACGGTGGTGGCGCATTCTCAGGAAAAGA
ACCATCTAAAGTTGACCGTTCTGCGGCTTATGCTGCACGTTATGTCGCAAAAAATATTGTGGCAGCAGGTTTAGCAGATC
GTTGTGAAATCCAACTTTCTTATGCGATTGGTGTGGCTGAACCAACATCTATCATGGTCGAAACCTTTGGAACTGGAAAA
GTTGCGAATGAATTATTAGTGTCATTAGTTCGTGAGTTTTTTGATTTACGTCCTTATGGCTTAATTAAAATGTTAGATTT
AATCCAACCAATCTATCGTGAAACGGCAGCTTATGGTCATTTCGGTCGTGAACAATTCCCTTGGGAAAAAGTGGATCGCG
CAGAAGATTTACGTATTGCGGCAGGTTTAAAATAA

Upstream 100 bases:

>100_bases
CACAACTAACCCATAATACCGCTTGCTTATTAAGCAAAAATGCGTATTATAGCCGTCTATACGTCAATACATCCAAATTT
AAAAGAAGAAGAGAGAAAAT

Downstream 100 bases:

>100_bases
TAGGCTTGAATTTTTAGTTTGAAAGGATTATATTGGAAAGCAACAAGGTTTCTTGTTGCCTTAGTTGGTTTAGGTTGCTA
CCAAGCTGGGGAGCTAAAGG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MSSYLFTSESVSEGHPDKIADQISDAVLDEILKQDPKARVACETYVKTGMALVGGEITTSAWVDIENLTRKVICDIGYEH
SEMGFDGHSCAVLNAIGKQSADINQGVDRENPLDQGAGDQGIMFGYATNETDVLMPAAITYAHRLMEKQSEVRKSGKLAW
LRPDAKSQVTLKYEDNKIVGVDAVVLSTQHSEEVSQKDLHEGVMEEIIKPVLPSEWLSKETKFFINPTGRFVIGGPMGDC
GLTGRKIIVDTYGGAARHGGGAFSGKEPSKVDRSAAYAARYVAKNIVAAGLADRCEIQLSYAIGVAEPTSIMVETFGTGK
VANELLVSLVREFFDLRPYGLIKMLDLIQPIYRETAAYGHFGREQFPWEKVDRAEDLRIAAGLK

Sequences:

>Translated_384_residues
MSSYLFTSESVSEGHPDKIADQISDAVLDEILKQDPKARVACETYVKTGMALVGGEITTSAWVDIENLTRKVICDIGYEH
SEMGFDGHSCAVLNAIGKQSADINQGVDRENPLDQGAGDQGIMFGYATNETDVLMPAAITYAHRLMEKQSEVRKSGKLAW
LRPDAKSQVTLKYEDNKIVGVDAVVLSTQHSEEVSQKDLHEGVMEEIIKPVLPSEWLSKETKFFINPTGRFVIGGPMGDC
GLTGRKIIVDTYGGAARHGGGAFSGKEPSKVDRSAAYAARYVAKNIVAAGLADRCEIQLSYAIGVAEPTSIMVETFGTGK
VANELLVSLVREFFDLRPYGLIKMLDLIQPIYRETAAYGHFGREQFPWEKVDRAEDLRIAAGLK
>Mature_383_residues
SSYLFTSESVSEGHPDKIADQISDAVLDEILKQDPKARVACETYVKTGMALVGGEITTSAWVDIENLTRKVICDIGYEHS
EMGFDGHSCAVLNAIGKQSADINQGVDRENPLDQGAGDQGIMFGYATNETDVLMPAAITYAHRLMEKQSEVRKSGKLAWL
RPDAKSQVTLKYEDNKIVGVDAVVLSTQHSEEVSQKDLHEGVMEEIIKPVLPSEWLSKETKFFINPTGRFVIGGPMGDCG
LTGRKIIVDTYGGAARHGGGAFSGKEPSKVDRSAAYAARYVAKNIVAAGLADRCEIQLSYAIGVAEPTSIMVETFGTGKV
ANELLVSLVREFFDLRPYGLIKMLDLIQPIYRETAAYGHFGREQFPWEKVDRAEDLRIAAGLK

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=373, Percent_Identity=55.7640750670241, Blast_Score=403, Evalue=1e-112,
Organism=Homo sapiens, GI4557737, Length=372, Percent_Identity=55.9139784946236, Blast_Score=402, Evalue=1e-112,
Organism=Escherichia coli, GI1789311, Length=384, Percent_Identity=83.0729166666667, Blast_Score=675, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17538498, Length=386, Percent_Identity=55.9585492227979, Blast_Score=411, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17551082, Length=387, Percent_Identity=54.5219638242894, Blast_Score=404, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI25145633, Length=388, Percent_Identity=55.1546391752577, Blast_Score=404, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17538494, Length=373, Percent_Identity=57.1045576407507, Blast_Score=403, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI32565905, Length=373, Percent_Identity=50.6702412868633, Blast_Score=343, Evalue=8e-95,
Organism=Caenorhabditis elegans, GI32565911, Length=340, Percent_Identity=52.9411764705882, Blast_Score=338, Evalue=3e-93,
Organism=Caenorhabditis elegans, GI25145635, Length=340, Percent_Identity=52.3529411764706, Blast_Score=329, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI32565903, Length=303, Percent_Identity=53.1353135313531, Blast_Score=302, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI32565909, Length=84, Percent_Identity=63.0952380952381, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI32565907, Length=45, Percent_Identity=71.1111111111111, Blast_Score=71, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6323209, Length=373, Percent_Identity=56.5683646112601, Blast_Score=412, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6320710, Length=378, Percent_Identity=55.2910052910053, Blast_Score=409, Evalue=1e-115,
Organism=Drosophila melanogaster, GI45552159, Length=374, Percent_Identity=55.0802139037433, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580529, Length=374, Percent_Identity=55.0802139037433, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580537, Length=374, Percent_Identity=55.0802139037433, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580533, Length=374, Percent_Identity=55.0802139037433, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580535, Length=374, Percent_Identity=55.0802139037433, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580531, Length=374, Percent_Identity=55.0802139037433, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580527, Length=375, Percent_Identity=55.4666666666667, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580525, Length=375, Percent_Identity=55.4666666666667, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580541, Length=374, Percent_Identity=53.475935828877, Blast_Score=355, Evalue=4e-98,
Organism=Drosophila melanogaster, GI24580539, Length=121, Percent_Identity=56.198347107438, Blast_Score=125, Evalue=3e-29,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 41974; Mature: 41843

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSYLFTSESVSEGHPDKIADQISDAVLDEILKQDPKARVACETYVKTGMALVGGEITTS
CCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHCCCCCCHH
AWVDIENLTRKVICDIGYEHSEMGFDGHSCAVLNAIGKQSADINQGVDRENPLDQGAGDQ
HEEEHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCC
GIMFGYATNETDVLMPAAITYAHRLMEKQSEVRKSGKLAWLRPDAKSQVTLKYEDNKIVG
CEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEECCCEEEE
VDAVVLSTQHSEEVSQKDLHEGVMEEIIKPVLPSEWLSKETKFFINPTGRFVIGGPMGDC
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEECCCCCEEECCCCCCC
GLTGRKIIVDTYGGAARHGGGAFSGKEPSKVDRSAAYAARYVAKNIVAAGLADRCEIQLS
CCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
YAIGVAEPTSIMVETFGTGKVANELLVSLVREFFDLRPYGLIKMLDLIQPIYRETAAYGH
EEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
FGREQFPWEKVDRAEDLRIAAGLK
CCCCCCCHHHHCCHHCCEEECCCC
>Mature Secondary Structure 
SSYLFTSESVSEGHPDKIADQISDAVLDEILKQDPKARVACETYVKTGMALVGGEITTS
CCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHCCCCCCHH
AWVDIENLTRKVICDIGYEHSEMGFDGHSCAVLNAIGKQSADINQGVDRENPLDQGAGDQ
HEEEHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCC
GIMFGYATNETDVLMPAAITYAHRLMEKQSEVRKSGKLAWLRPDAKSQVTLKYEDNKIVG
CEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEECCCEEEE
VDAVVLSTQHSEEVSQKDLHEGVMEEIIKPVLPSEWLSKETKFFINPTGRFVIGGPMGDC
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEECCCCCEEECCCCCCC
GLTGRKIIVDTYGGAARHGGGAFSGKEPSKVDRSAAYAARYVAKNIVAAGLADRCEIQLS
CCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
YAIGVAEPTSIMVETFGTGKVANELLVSLVREFFDLRPYGLIKMLDLIQPIYRETAAYGH
EEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
FGREQFPWEKVDRAEDLRIAAGLK
CCCCCCCHHHHCCHHCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA