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The map label for this gene is murF

Identifier: 16273060

GI number: 16273060

Start: 1201162

End: 1202535

Strand: Direct

Name: murF

Synonym: HI1134

Alternate gene names: 16273060

Gene position: 1201162-1202535 (Clockwise)

Preceding gene: 16273059

Following gene: 16273061

Centisome position: 65.63

GC content: 37.12

Gene sequence:

>1374_bases
ATGATTAAACTCTCTACTGTACAACTTGCACAGATTCTTCAAGCTAAATTAATTGGCGATGAAAATGTGCAAGTTGAAAA
AATTAATACGGATACTCGAAAAAGCGTATCAAATAGCTTATTTTTTGCTTTAAAAGGCGAAAAATTTGATGCTCATCAAT
ATCTTGATCAAGCGGTGTCACAGGGTGCGCTTGCGCTTGTTGTTCAGCAAGAAAATTCATCCATTTCTGTTCCACAATTG
GTGGTTAAGGATACCCGTATTGCCTTGGGTGAACTTGCAAAATGGTTACGTGAGAAAATTAATCCACGTACTGTGGCGAT
GACCGGCTCTTCTGGTAAAACCACGGTAAAAGAAATGACGGCAAGTATTTTGCAACATACCGCAGCGGATTCAGAAGCGG
TACTTTTTACCAATGGTAACTTCAATAACGATATAGGTGTGCCTTTAACTCTACTTCGCTTAACAGAAAAACATCGTTTT
GCTGTGATTGAACTTGGTGCAAATCATCAAAATGAAATCAATTACACCACAAAATTAGTTCAACCTAATGCCGCATTGAT
TAATAATATTGCGCCCGCACATTTAGAGGGGTTTGGTTCTTTAGCGGGGGTTGTTCAAGCAAAAGGCGAGATTTATCGAG
GTTTAACAAAAAATGGTGTGGCGATTATCAATGCTGAACATAATCACTTAGATATTTGGCAAAAAGAAATCAGTAATCAC
GCCATTCAATATTTCAACGGTAAAGATTATTCTGCAAAAAATATTCATCATACTTCTCAAGGCTCCACTTTTACACTTAT
TTCCCCACAAGGTGAAATTGAGATTACCTTACCTTACCTTGGTGAACATAATGTAAAAAATGCCTTGGCAGCGACCGCAC
TTGCAATGAATGTCGGTGCCACTCTTACAGATGTAAAAGCTGGATTAGAACAACGTTCACAAGTGAAAGGGCGTTTATTC
CCCATTCAAGTCACGCCTAATTTATTGCTGTTAGATGATACTTATAATGCGAATAAAGATTCTCTGTGTGCTGCGATTGA
TGTTTTAAAAGGTTATGATGCTTTCCGTATTTTGTGTGTCGGTGATATGAAAGAGCTAGGCGAAAACTCTCTCGCTATTC
ATCGTGAAGTTGGACAATATATTAATTTGGTAAATTTAGATTTAGTGTGTTCTTATGGCAATGAAAGTGCGGTTATTTCT
GAGGCTGTTTCGGGTAAGCATTTTACGGATAAAACTGAAATGGTGGATTTCTTAGTTCCACTTATTGAAAACCAATTACA
ACAAAATAAAAAAGTTGTGGTGCTAGGAAAAGGCTCACGCTCAATGAAAATGGAAGATGTGATCTATTCATTAAAGGATA
AAATTAAATGTTAG

Upstream 100 bases:

>100_bases
GGAAAGGGGCACGAGCATTATCAAATTATTGGTTCGGAAGTTGTGCATTTTTCCGACCAAGAAATTGCACTTGATTTCTT
AAAATAATAATCACTCAATT

Downstream 100 bases:

>100_bases
TCTGGCTTGCTGAATATCTTGTTCGTTACGAAACCGCGTTTAATGCTATTTCTTATATTACAGTCCGCGCAAATCTTGCA
TTATTAACCGCACTTTTTAT

Product: UDP-MurNAc-pentapeptide synthetase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MIKLSTVQLAQILQAKLIGDENVQVEKINTDTRKSVSNSLFFALKGEKFDAHQYLDQAVSQGALALVVQQENSSISVPQL
VVKDTRIALGELAKWLREKINPRTVAMTGSSGKTTVKEMTASILQHTAADSEAVLFTNGNFNNDIGVPLTLLRLTEKHRF
AVIELGANHQNEINYTTKLVQPNAALINNIAPAHLEGFGSLAGVVQAKGEIYRGLTKNGVAIINAEHNHLDIWQKEISNH
AIQYFNGKDYSAKNIHHTSQGSTFTLISPQGEIEITLPYLGEHNVKNALAATALAMNVGATLTDVKAGLEQRSQVKGRLF
PIQVTPNLLLLDDTYNANKDSLCAAIDVLKGYDAFRILCVGDMKELGENSLAIHREVGQYINLVNLDLVCSYGNESAVIS
EAVSGKHFTDKTEMVDFLVPLIENQLQQNKKVVVLGKGSRSMKMEDVIYSLKDKIKC

Sequences:

>Translated_457_residues
MIKLSTVQLAQILQAKLIGDENVQVEKINTDTRKSVSNSLFFALKGEKFDAHQYLDQAVSQGALALVVQQENSSISVPQL
VVKDTRIALGELAKWLREKINPRTVAMTGSSGKTTVKEMTASILQHTAADSEAVLFTNGNFNNDIGVPLTLLRLTEKHRF
AVIELGANHQNEINYTTKLVQPNAALINNIAPAHLEGFGSLAGVVQAKGEIYRGLTKNGVAIINAEHNHLDIWQKEISNH
AIQYFNGKDYSAKNIHHTSQGSTFTLISPQGEIEITLPYLGEHNVKNALAATALAMNVGATLTDVKAGLEQRSQVKGRLF
PIQVTPNLLLLDDTYNANKDSLCAAIDVLKGYDAFRILCVGDMKELGENSLAIHREVGQYINLVNLDLVCSYGNESAVIS
EAVSGKHFTDKTEMVDFLVPLIENQLQQNKKVVVLGKGSRSMKMEDVIYSLKDKIKC
>Mature_457_residues
MIKLSTVQLAQILQAKLIGDENVQVEKINTDTRKSVSNSLFFALKGEKFDAHQYLDQAVSQGALALVVQQENSSISVPQL
VVKDTRIALGELAKWLREKINPRTVAMTGSSGKTTVKEMTASILQHTAADSEAVLFTNGNFNNDIGVPLTLLRLTEKHRF
AVIELGANHQNEINYTTKLVQPNAALINNIAPAHLEGFGSLAGVVQAKGEIYRGLTKNGVAIINAEHNHLDIWQKEISNH
AIQYFNGKDYSAKNIHHTSQGSTFTLISPQGEIEITLPYLGEHNVKNALAATALAMNVGATLTDVKAGLEQRSQVKGRLF
PIQVTPNLLLLDDTYNANKDSLCAAIDVLKGYDAFRILCVGDMKELGENSLAIHREVGQYINLVNLDLVCSYGNESAVIS
EAVSGKHFTDKTEMVDFLVPLIENQLQQNKKVVVLGKGSRSMKMEDVIYSLKDKIKC

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786274, Length=462, Percent_Identity=50.8658008658009, Blast_Score=416, Evalue=1e-117,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURF_HAEIN (P45061)

Other databases:

- EMBL:   L42023
- PIR:   I64184
- RefSeq:   NP_439292.1
- ProteinModelPortal:   P45061
- GeneID:   950620
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1134
- NMPDR:   fig|71421.1.peg.1087
- TIGR:   HI_1134
- HOGENOM:   HBG617121
- OMA:   ANPASMR
- ProtClustDB:   CLSK869981
- BioCyc:   HINF71421:HI_1134-MONOMER
- BRENDA:   6.3.2.10
- GO:   GO:0005737
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- PANTHER:   PTHR23135:SF3
- TIGRFAMs:   TIGR01143

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.10

Molecular weight: Translated: 50074; Mature: 50074

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLSTVQLAQILQAKLIGDENVQVEKINTDTRKSVSNSLFFALKGEKFDAHQYLDQAVS
CEEECHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHCCCEEEEEECCCCCHHHHHHHHHC
QGALALVVQQENSSISVPQLVVKDTRIALGELAKWLREKINPRTVAMTGSSGKTTVKEMT
CCEEEEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHH
ASILQHTAADSEAVLFTNGNFNNDIGVPLTLLRLTEKHRFAVIELGANHQNEINYTTKLV
HHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCEEEEEE
QPNAALINNIAPAHLEGFGSLAGVVQAKGEIYRGLTKNGVAIINAEHNHLDIWQKEISNH
CCCHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEEECCCCCHHHHHHHHCCC
AIQYFNGKDYSAKNIHHTSQGSTFTLISPQGEIEITLPYLGEHNVKNALAATALAMNVGA
CEEEECCCCCCCCCCEECCCCCEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCC
TLTDVKAGLEQRSQVKGRLFPIQVTPNLLLLDDTYNANKDSLCAAIDVLKGYDAFRILCV
HHHHHHHHHHHHHHHCCEEEEEEECCCEEEEECCCCCCCCHHHHHHHHHCCCCEEEEEEE
GDMKELGENSLAIHREVGQYINLVNLDLVCSYGNESAVISEAVSGKHFTDKTEMVDFLVP
CCHHHCCCCCEEHHHHHHHHHCEEEEEEEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHH
LIENQLQQNKKVVVLGKGSRSMKMEDVIYSLKDKIKC
HHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKLSTVQLAQILQAKLIGDENVQVEKINTDTRKSVSNSLFFALKGEKFDAHQYLDQAVS
CEEECHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHCCCEEEEEECCCCCHHHHHHHHHC
QGALALVVQQENSSISVPQLVVKDTRIALGELAKWLREKINPRTVAMTGSSGKTTVKEMT
CCEEEEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHH
ASILQHTAADSEAVLFTNGNFNNDIGVPLTLLRLTEKHRFAVIELGANHQNEINYTTKLV
HHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCEEEEEE
QPNAALINNIAPAHLEGFGSLAGVVQAKGEIYRGLTKNGVAIINAEHNHLDIWQKEISNH
CCCHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEEECCCCCHHHHHHHHCCC
AIQYFNGKDYSAKNIHHTSQGSTFTLISPQGEIEITLPYLGEHNVKNALAATALAMNVGA
CEEEECCCCCCCCCCEECCCCCEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCC
TLTDVKAGLEQRSQVKGRLFPIQVTPNLLLLDDTYNANKDSLCAAIDVLKGYDAFRILCV
HHHHHHHHHHHHHHHCCEEEEEEECCCEEEEECCCCCCCCHHHHHHHHHCCCCEEEEEEE
GDMKELGENSLAIHREVGQYINLVNLDLVCSYGNESAVISEAVSGKHFTDKTEMVDFLVP
CCHHHCCCCCEEHHHHHHHHHCEEEEEEEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHH
LIENQLQQNKKVVVLGKGSRSMKMEDVIYSLKDKIKC
HHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800