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The map label for this gene is bioB

Identifier: 16272956

GI number: 16272956

Start: 1086136

End: 1087137

Strand: Direct

Name: bioB

Synonym: HI1022

Alternate gene names: 16272956

Gene position: 1086136-1087137 (Clockwise)

Preceding gene: 16272955

Following gene: 16272966

Centisome position: 59.35

GC content: 41.72

Gene sequence:

>1002_bases
ATGTTAGCGGAAAAATTACAAATTAACTCTATTACACCTCATCCAAGTGTAGAATATTGGTCTGTTTGCAAAGTTGAGGC
GTTATTTGAAACGCCATTTTTGGAATTAGTTTACCGAGCAACGCAAGTTCATCGTAAGCATTTTAATCCTCGCGCGATTC
AGTTATCCACGTTAATGTCTATCAAAACGGGGGGATGCCCAGAAGATTGTAGTTATTGCCCTCAATCAGCCCGTTATCAT
ACTGGCGTACAAAATCAGCAGTTATTAGATGTTGATGAGATCGTCGCTAAAGCAAAAATTGCGAAAGCACGTGGTGCAGG
GCGTTTCTGTATGGGGGCTGCTTGGCGAGGCCCTAAGCCAAAAGATATTGAGAAAGTCACAGAAATTATTAAAGCCGTGA
AATCACTTGGTTTAGAAACTTGCGGTACCTTTGGTTTATTGCAAGACGGTATGGCGGAAGATTTAAAAGAAGCTGGATTA
GATTATTACAATCATAATCTTGATACCGCACCAGAACATTATGCAGAAGTGATTGGTACTCGCCGTTTTGATGATCGCCT
TAGCACCTTAGGAAAAGTGCGCAAAGCTGGATTAAAAGTGTGTTGTGGTGGCATTGTAGGGATGAATGAAACTCGCAAAG
AACGGGCTGGATTAATCGCGAGCCTTGCAAATCTTGATCCGCAACCTGAGTCAGTGCCGATTAATCAACTCGTTAAGGTT
GAAGGTACGCCGTTAGCCGATGCGGAAGAATTAGATTGGACGGAGTTTGTGCGAACGATTGCTGTTGCACGTATTACTAT
GCCAAAAAGTTATGTTCGCCTTTCTGCAGGTCGCAGTGGTATGACTGAAGAAATGCAAGCGATGTGTTTTATGGCGGGTG
CAAATTCTATTTTCTATGGCGATAAATTACTTGTTACAGATAATCCAGAAGAAGATGGCGATCAGTTATTGATGGCAAAA
TTAGATTTAGAGCCAGAAACTGCAGAAAATAAAAAGTTGTAG

Upstream 100 bases:

>100_bases
TCATAACGTTGAATTGATTGTTTCTACCATATACACTAATTCATCAATTAAGAAAGTATAAAAAGCGTGGTGTTTTTAAC
CGCACTTTCAAAGGAAAAAC

Downstream 100 bases:

>100_bases
TTTGCGTTAGAAAGCTGAGAAATTAAGTATAAATCTATTCCTTTCTTTATTTCATATCTCACAATAATTTTCAATAGCCA
ATAATAAAGCTCCTTGAATA

Product: biotin synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MLAEKLQINSITPHPSVEYWSVCKVEALFETPFLELVYRATQVHRKHFNPRAIQLSTLMSIKTGGCPEDCSYCPQSARYH
TGVQNQQLLDVDEIVAKAKIAKARGAGRFCMGAAWRGPKPKDIEKVTEIIKAVKSLGLETCGTFGLLQDGMAEDLKEAGL
DYYNHNLDTAPEHYAEVIGTRRFDDRLSTLGKVRKAGLKVCCGGIVGMNETRKERAGLIASLANLDPQPESVPINQLVKV
EGTPLADAEELDWTEFVRTIAVARITMPKSYVRLSAGRSGMTEEMQAMCFMAGANSIFYGDKLLVTDNPEEDGDQLLMAK
LDLEPETAENKKL

Sequences:

>Translated_333_residues
MLAEKLQINSITPHPSVEYWSVCKVEALFETPFLELVYRATQVHRKHFNPRAIQLSTLMSIKTGGCPEDCSYCPQSARYH
TGVQNQQLLDVDEIVAKAKIAKARGAGRFCMGAAWRGPKPKDIEKVTEIIKAVKSLGLETCGTFGLLQDGMAEDLKEAGL
DYYNHNLDTAPEHYAEVIGTRRFDDRLSTLGKVRKAGLKVCCGGIVGMNETRKERAGLIASLANLDPQPESVPINQLVKV
EGTPLADAEELDWTEFVRTIAVARITMPKSYVRLSAGRSGMTEEMQAMCFMAGANSIFYGDKLLVTDNPEEDGDQLLMAK
LDLEPETAENKKL
>Mature_333_residues
MLAEKLQINSITPHPSVEYWSVCKVEALFETPFLELVYRATQVHRKHFNPRAIQLSTLMSIKTGGCPEDCSYCPQSARYH
TGVQNQQLLDVDEIVAKAKIAKARGAGRFCMGAAWRGPKPKDIEKVTEIIKAVKSLGLETCGTFGLLQDGMAEDLKEAGL
DYYNHNLDTAPEHYAEVIGTRRFDDRLSTLGKVRKAGLKVCCGGIVGMNETRKERAGLIASLANLDPQPESVPINQLVKV
EGTPLADAEELDWTEFVRTIAVARITMPKSYVRLSAGRSGMTEEMQAMCFMAGANSIFYGDKLLVTDNPEEDGDQLLMAK
LDLEPETAENKKL

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=307, Percent_Identity=59.9348534201954, Blast_Score=403, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6321725, Length=314, Percent_Identity=46.1783439490446, Blast_Score=315, Evalue=7e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_HAEI8 (Q4QLQ0)

Other databases:

- EMBL:   CP000057
- RefSeq:   YP_248707.1
- ProteinModelPortal:   Q4QLQ0
- SMR:   Q4QLQ0
- STRING:   Q4QLQ0
- GeneID:   3430472
- GenomeReviews:   CP000057_GR
- KEGG:   hit:NTHI1188
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- PhylomeDB:   Q4QLQ0
- ProtClustDB:   CLSK2299228
- BioCyc:   HINF281310:NTHI1188-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 36786; Mature: 36786

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAEKLQINSITPHPSVEYWSVCKVEALFETPFLELVYRATQVHRKHFNPRAIQLSTLMS
CCCCCEECCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEEHHHHHH
IKTGGCPEDCSYCPQSARYHTGVQNQQLLDVDEIVAKAKIAKARGAGRFCMGAAWRGPKP
HCCCCCCHHHHHCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCC
KDIEKVTEIIKAVKSLGLETCGTFGLLQDGMAEDLKEAGLDYYNHNLDTAPEHYAEVIGT
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHC
RRFDDRLSTLGKVRKAGLKVCCGGIVGMNETRKERAGLIASLANLDPQPESVPINQLVKV
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHEEEE
EGTPLADAEELDWTEFVRTIAVARITMPKSYVRLSAGRSGMTEEMQAMCFMAGANSIFYG
CCCCCCCHHHCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEC
DKLLVTDNPEEDGDQLLMAKLDLEPETAENKKL
CEEEEECCCCCCCHHEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MLAEKLQINSITPHPSVEYWSVCKVEALFETPFLELVYRATQVHRKHFNPRAIQLSTLMS
CCCCCEECCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEEHHHHHH
IKTGGCPEDCSYCPQSARYHTGVQNQQLLDVDEIVAKAKIAKARGAGRFCMGAAWRGPKP
HCCCCCCHHHHHCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCC
KDIEKVTEIIKAVKSLGLETCGTFGLLQDGMAEDLKEAGLDYYNHNLDTAPEHYAEVIGT
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHC
RRFDDRLSTLGKVRKAGLKVCCGGIVGMNETRKERAGLIASLANLDPQPESVPINQLVKV
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHEEEE
EGTPLADAEELDWTEFVRTIAVARITMPKSYVRLSAGRSGMTEEMQAMCFMAGANSIFYG
CCCCCCCHHHCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEC
DKLLVTDNPEEDGDQLLMAKLDLEPETAENKKL
CEEEEECCCCCCCHHEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA