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The map label for this gene is trmE [H]

Identifier: 16272938

GI number: 16272938

Start: 1066325

End: 1067710

Strand: Direct

Name: trmE [H]

Synonym: HI1002

Alternate gene names: 16272938

Gene position: 1066325-1067710 (Clockwise)

Preceding gene: 16272937

Following gene: 30995417

Centisome position: 58.26

GC content: 39.61

Gene sequence:

>1386_bases
GTGCGGCTAGATTTTAAAGAGTTTTTTATGAAAGAAACAATCGTTGCTCAAGCTACTGCACCAGGTCGTGGTGGTATTGG
AATTTTAAGAGTCTCTGGGCCTTTAGCAACAAAAGTTGCTCAAGCTATATTAGGTAAATGCCCGAAGCCAAGAATGGCAG
ATTATCTTCCTTTTAAAGATGCTGATGGCACTATTTTAGATCAAGGGATTGCGCTTTATTTCAAATCGCCAAATTCATTT
ACTGGTGAAGATGTGTTGGAATTGCAAGGTCACGGTGGGCAGGTTGTATTAGATTTATTACTCAAACGTATTTTACAAAT
TGATGGAATCCGTTTGGCGAGACCAGGCGAGTTTTCTGAACAAGCTTTTTTGAATGATAAATTGGATCTTGCTCAAGCGG
AGGCCATTGCGGATTTAATTGATGCAACATCAGAACAAGCGGTACGCTCAGCATTAAAATCTTTGCAAGGTGAATTTTCT
AAAAAAGTAAATGAATTAGTTGATTCTGTGATTTACTTACGTACTTATGTAGAAGCAAGCATTGACTTTCCTGATGAAGA
AATAGATTTCTTGGCAGATGGAAAAATTGAAGCAAACTTACGTGGTATTATTAATCAGTTGGAAGATGTTCGCTCTGAAG
CTAAACAAGGTTCTATTTTACGTGAGGGAATGAAAGTCGTGATTGCTGGTCGTCCGAATGCGGGTAAATCGAGCTTGCTT
AATGCGCTTGCTGGTCGCGAAGCGGCTATCGTCACGGATATTGCAGGTACAACCCGTGATGTATTGCGTGAGCATATTCA
TATTGACGGTATGCCATTGCATATTATTGATACCGCAGGTCTTCGAGATGCAATCGATGAAGTAGAGCGTATTGGGATTT
CTCGTGCTTGGACAGAGATTGAACAAGCCGATCGTATTATTTTGATGTTAGATAGTAGCGATCCTGAAAGTGCAGATTTA
AGCAAAGTGCGGTCAGAATTTTTAGCAAAATTACCCTCAACCTTGCCAGTTACTATTGTTAGAAACAAAATTGATTTAAA
TGGCGAACAAGCAAGTGAAAGCGAACAGGGCGGTTATCAAATGATTAGTCTTTCCGCTCAAACGCATGATGGTGTACAAC
TTTTACGTGAGCATTTGAAACAAGCAATGGGTTTCCAAACAGGCATGGAAGGAGGCTTTTTAGCTCGTCGTCGTCATTTA
GATGCGCTGGATAAAGCCGCAGAGCATTTACAAATCGGTTTGGTTCAACTTACAGAATTTCACGCAGGAGAACTTTTAGC
GGAAGAATTACGCTTAGTTCAAAGCTATTTAAGTGAAATTACGGGTCAATTCACATCTGATGATTTACTTGGTAATATTT
TTAGTTCTTTCTGCATTGGAAAATAG

Upstream 100 bases:

>100_bases
TGTGCTTTCTTTATTATTTGATCTTAATATATTCTGCATTTTGTAGAGATAGAGTTTTTTTAGTAGAAACAGGGATAAGC
CTTATCCCTCTAAATTAAAA

Downstream 100 bases:

>100_bases
GAAGCGTAAAGTGCGGTCATTTTTTGGCAAAATTTAAATCAAAAGCGGCAATATTTGGTGGTGTTTTGTTGATAGGAATT
GCTATTAGCTGGCTTTGTGA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MRLDFKEFFMKETIVAQATAPGRGGIGILRVSGPLATKVAQAILGKCPKPRMADYLPFKDADGTILDQGIALYFKSPNSF
TGEDVLELQGHGGQVVLDLLLKRILQIDGIRLARPGEFSEQAFLNDKLDLAQAEAIADLIDATSEQAVRSALKSLQGEFS
KKVNELVDSVIYLRTYVEASIDFPDEEIDFLADGKIEANLRGIINQLEDVRSEAKQGSILREGMKVVIAGRPNAGKSSLL
NALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRDAIDEVERIGISRAWTEIEQADRIILMLDSSDPESADL
SKVRSEFLAKLPSTLPVTIVRNKIDLNGEQASESEQGGYQMISLSAQTHDGVQLLREHLKQAMGFQTGMEGGFLARRRHL
DALDKAAEHLQIGLVQLTEFHAGELLAEELRLVQSYLSEITGQFTSDDLLGNIFSSFCIGK

Sequences:

>Translated_461_residues
MRLDFKEFFMKETIVAQATAPGRGGIGILRVSGPLATKVAQAILGKCPKPRMADYLPFKDADGTILDQGIALYFKSPNSF
TGEDVLELQGHGGQVVLDLLLKRILQIDGIRLARPGEFSEQAFLNDKLDLAQAEAIADLIDATSEQAVRSALKSLQGEFS
KKVNELVDSVIYLRTYVEASIDFPDEEIDFLADGKIEANLRGIINQLEDVRSEAKQGSILREGMKVVIAGRPNAGKSSLL
NALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRDAIDEVERIGISRAWTEIEQADRIILMLDSSDPESADL
SKVRSEFLAKLPSTLPVTIVRNKIDLNGEQASESEQGGYQMISLSAQTHDGVQLLREHLKQAMGFQTGMEGGFLARRRHL
DALDKAAEHLQIGLVQLTEFHAGELLAEELRLVQSYLSEITGQFTSDDLLGNIFSSFCIGK
>Mature_461_residues
MRLDFKEFFMKETIVAQATAPGRGGIGILRVSGPLATKVAQAILGKCPKPRMADYLPFKDADGTILDQGIALYFKSPNSF
TGEDVLELQGHGGQVVLDLLLKRILQIDGIRLARPGEFSEQAFLNDKLDLAQAEAIADLIDATSEQAVRSALKSLQGEFS
KKVNELVDSVIYLRTYVEASIDFPDEEIDFLADGKIEANLRGIINQLEDVRSEAKQGSILREGMKVVIAGRPNAGKSSLL
NALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRDAIDEVERIGISRAWTEIEQADRIILMLDSSDPESADL
SKVRSEFLAKLPSTLPVTIVRNKIDLNGEQASESEQGGYQMISLSAQTHDGVQLLREHLKQAMGFQTGMEGGFLARRRHL
DALDKAAEHLQIGLVQLTEFHAGELLAEELRLVQSYLSEITGQFTSDDLLGNIFSSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=467, Percent_Identity=35.117773019272, Blast_Score=231, Evalue=1e-60,
Organism=Homo sapiens, GI193082988, Length=499, Percent_Identity=33.0661322645291, Blast_Score=219, Evalue=4e-57,
Organism=Homo sapiens, GI193082993, Length=461, Percent_Identity=32.3210412147505, Blast_Score=207, Evalue=2e-53,
Organism=Escherichia coli, GI2367268, Length=451, Percent_Identity=74.2793791574279, Blast_Score=681, Evalue=0.0,
Organism=Escherichia coli, GI87082120, Length=94, Percent_Identity=38.2978723404255, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17507259, Length=451, Percent_Identity=28.3813747228381, Blast_Score=161, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6323665, Length=498, Percent_Identity=32.5301204819277, Blast_Score=209, Evalue=1e-54,
Organism=Drosophila melanogaster, GI45550813, Length=472, Percent_Identity=31.3559322033898, Blast_Score=210, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 50447; Mature: 50447

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDFKEFFMKETIVAQATAPGRGGIGILRVSGPLATKVAQAILGKCPKPRMADYLPFKD
CCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCC
ADGTILDQGIALYFKSPNSFTGEDVLELQGHGGQVVLDLLLKRILQIDGIRLARPGEFSE
CCCCEECCCEEEEEECCCCCCCCCHHEECCCCCHHHHHHHHHHHHHHCCEEECCCCCCCH
QAFLNDKLDLAQAEAIADLIDATSEQAVRSALKSLQGEFSKKVNELVDSVIYLRTYVEAS
HHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
IDFPDEEIDFLADGKIEANLRGIINQLEDVRSEAKQGSILREGMKVVIAGRPNAGKSSLL
CCCCCHHHCEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHHHHH
NALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRDAIDEVERIGISRAWTEI
HHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCHHHHHHHH
EQADRIILMLDSSDPESADLSKVRSEFLAKLPSTLPVTIVRNKIDLNGEQASESEQGGYQ
HHCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEECEECCCCCCCCCCCCCCEE
MISLSAQTHDGVQLLREHLKQAMGFQTGMEGGFLARRRHLDALDKAAEHLQIGLVQLTEF
EEEEECCCCHHHHHHHHHHHHHHCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HAGELLAEELRLVQSYLSEITGQFTSDDLLGNIFSSFCIGK
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRLDFKEFFMKETIVAQATAPGRGGIGILRVSGPLATKVAQAILGKCPKPRMADYLPFKD
CCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCC
ADGTILDQGIALYFKSPNSFTGEDVLELQGHGGQVVLDLLLKRILQIDGIRLARPGEFSE
CCCCEECCCEEEEEECCCCCCCCCHHEECCCCCHHHHHHHHHHHHHHCCEEECCCCCCCH
QAFLNDKLDLAQAEAIADLIDATSEQAVRSALKSLQGEFSKKVNELVDSVIYLRTYVEAS
HHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
IDFPDEEIDFLADGKIEANLRGIINQLEDVRSEAKQGSILREGMKVVIAGRPNAGKSSLL
CCCCCHHHCEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHHHHH
NALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRDAIDEVERIGISRAWTEI
HHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCHHHHHHHH
EQADRIILMLDSSDPESADLSKVRSEFLAKLPSTLPVTIVRNKIDLNGEQASESEQGGYQ
HHCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEECEECCCCCCCCCCCCCCEE
MISLSAQTHDGVQLLREHLKQAMGFQTGMEGGFLARRRHLDALDKAAEHLQIGLVQLTEF
EEEEECCCCHHHHHHHHHHHHHHCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HAGELLAEELRLVQSYLSEITGQFTSDDLLGNIFSSFCIGK
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA