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The map label for this gene is recF [H]

Identifier: 16272929

GI number: 16272929

Start: 1053715

End: 1054794

Strand: Reverse

Name: recF [H]

Synonym: HI0991

Alternate gene names: 16272929

Gene position: 1054794-1053715 (Counterclockwise)

Preceding gene: 16272930

Following gene: 16272928

Centisome position: 57.63

GC content: 39.91

Gene sequence:

>1080_bases
ATGGCGATTTCTCGTTTACTGGTCGAAAAATTTCGTAATTTAACAGCAGTGGATCTTGATTTTGATCCCTGCTTTAACTT
TTTAATCGGCAACAACGGCAGCGGAAAAACCAGCTTATTAGAAGCCATTTTTTATCTTGGTCACGGACGTTCATTTAAAA
GTGCGGTCACAAATCGCATCATTTCTTACGACGAACCGCACTTTACCCTATTTGGACAAATCCAAGAAAGCCAACATCAA
TGGTCTATCGGCTTACAAAAACTGCGTCAAGGCAACACCTTAGTAAAAATTAACGGCGAAGATGGCAATAAAATTTCCGA
TCTCGCCCATCTTTTACCAATGCAATTAATCACGCCAGAGGGCTTAACTTTACTCAACGGCGGGCCAAGTTATCGCCGCG
CGTTTTTAGATTGGGGATTATTCCATCACCAAACAAGTTTCTATTCCGCTTGGAGCAATCTCAATCGATTACTCAAACAA
CGAAATGCCGCCCTTGCACAAAATCAGCCTTACTCTGCCATAAAAATTTGGGACGTTGAACTTGCTAAACTTGCCCACCA
AGTAAGTGAATGGCGTGCCGAATACGCAGAAGCACTTAGCCCTGAAATTGAGCAAACTTGCCAACTTTTTCTGCCTGAAT
TAGAAATTAATGTCAGCTTTCATCAAGGATGGGAAAAAAATGCTGATTATTATGAAATTCTTCAACAAAATTTTGAACGA
GATCGTGCCTTAAATTACACCTTTTCAGGCCCTCAAAAAGCGGATTTTCGTTTCAAAGCACAAGGATTGCCAGTGGAAGA
TGTGCTTTCTCGTGGACAACTTAAACTTTTAATGTGTGCATTAAGACTAGCGCAGGGCGAGCATTTAATGAAAGAAAAGC
AACGCCATTGTATTTTTTTGATCGATGATTTTGCCTCGGAATTAGATCAATACAAACGTGCCTTGTTGGCAGAACGATTA
CAACAAAGCGGTTCACAAGTGTTTGTTACCGCCATTACGCAAAGACAACTAAAAGAAATGCAAGTGGAAAATAAAAAAAT
GTTTTCTGTTCATAATGGCATCATTAACGCCTTAAATTAG

Upstream 100 bases:

>100_bases
CAAGTGCGAATGTGCTTAACTGATGCGTTCTCCAGTTGCTTAATTGAAAACTGTGAAGACAGCAGCTGCGAATACGTCAT
TATGCCAATGCGTTTATAAT

Downstream 100 bases:

>100_bases
CCAAAAGTGCGGTGGAAAATACGCCAATTTTTTAAAAAATATCAAAAATTATTATCATTACCTCATAAATGTAATTCAAG
TTTTGAGCGATTTATGCTAT

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MAISRLLVEKFRNLTAVDLDFDPCFNFLIGNNGSGKTSLLEAIFYLGHGRSFKSAVTNRIISYDEPHFTLFGQIQESQHQ
WSIGLQKLRQGNTLVKINGEDGNKISDLAHLLPMQLITPEGLTLLNGGPSYRRAFLDWGLFHHQTSFYSAWSNLNRLLKQ
RNAALAQNQPYSAIKIWDVELAKLAHQVSEWRAEYAEALSPEIEQTCQLFLPELEINVSFHQGWEKNADYYEILQQNFER
DRALNYTFSGPQKADFRFKAQGLPVEDVLSRGQLKLLMCALRLAQGEHLMKEKQRHCIFLIDDFASELDQYKRALLAERL
QQSGSQVFVTAITQRQLKEMQVENKKMFSVHNGIINALN

Sequences:

>Translated_359_residues
MAISRLLVEKFRNLTAVDLDFDPCFNFLIGNNGSGKTSLLEAIFYLGHGRSFKSAVTNRIISYDEPHFTLFGQIQESQHQ
WSIGLQKLRQGNTLVKINGEDGNKISDLAHLLPMQLITPEGLTLLNGGPSYRRAFLDWGLFHHQTSFYSAWSNLNRLLKQ
RNAALAQNQPYSAIKIWDVELAKLAHQVSEWRAEYAEALSPEIEQTCQLFLPELEINVSFHQGWEKNADYYEILQQNFER
DRALNYTFSGPQKADFRFKAQGLPVEDVLSRGQLKLLMCALRLAQGEHLMKEKQRHCIFLIDDFASELDQYKRALLAERL
QQSGSQVFVTAITQRQLKEMQVENKKMFSVHNGIINALN
>Mature_358_residues
AISRLLVEKFRNLTAVDLDFDPCFNFLIGNNGSGKTSLLEAIFYLGHGRSFKSAVTNRIISYDEPHFTLFGQIQESQHQW
SIGLQKLRQGNTLVKINGEDGNKISDLAHLLPMQLITPEGLTLLNGGPSYRRAFLDWGLFHHQTSFYSAWSNLNRLLKQR
NAALAQNQPYSAIKIWDVELAKLAHQVSEWRAEYAEALSPEIEQTCQLFLPELEINVSFHQGWEKNADYYEILQQNFERD
RALNYTFSGPQKADFRFKAQGLPVEDVLSRGQLKLLMCALRLAQGEHLMKEKQRHCIFLIDDFASELDQYKRALLAERLQ
QSGSQVFVTAITQRQLKEMQVENKKMFSVHNGIINALN

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=356, Percent_Identity=57.3033707865169, Blast_Score=446, Evalue=1e-126,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 41195; Mature: 41063

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: PS00617 RECF_1 ; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAISRLLVEKFRNLTAVDLDFDPCFNFLIGNNGSGKTSLLEAIFYLGHGRSFKSAVTNRI
CCHHHHHHHHHCCCEEEECCHHHHHHHEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHH
ISYDEPHFTLFGQIQESQHQWSIGLQKLRQGNTLVKINGEDGNKISDLAHLLPMQLITPE
HCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCCC
GLTLLNGGPSYRRAFLDWGLFHHQTSFYSAWSNLNRLLKQRNAALAQNQPYSAIKIWDVE
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHH
LAKLAHQVSEWRAEYAEALSPEIEQTCQLFLPELEINVSFHQGWEKNADYYEILQQNFER
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHH
DRALNYTFSGPQKADFRFKAQGLPVEDVLSRGQLKLLMCALRLAQGEHLMKEKQRHCIFL
HHCCEEEECCCCCCCCEEEECCCCHHHHHCCCHHHHHHHHHHHHCCHHHHHHCCCCEEEE
IDDFASELDQYKRALLAERLQQSGSQVFVTAITQRQLKEMQVENKKMFSVHNGIINALN
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AISRLLVEKFRNLTAVDLDFDPCFNFLIGNNGSGKTSLLEAIFYLGHGRSFKSAVTNRI
CHHHHHHHHHCCCEEEECCHHHHHHHEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHH
ISYDEPHFTLFGQIQESQHQWSIGLQKLRQGNTLVKINGEDGNKISDLAHLLPMQLITPE
HCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCCC
GLTLLNGGPSYRRAFLDWGLFHHQTSFYSAWSNLNRLLKQRNAALAQNQPYSAIKIWDVE
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHH
LAKLAHQVSEWRAEYAEALSPEIEQTCQLFLPELEINVSFHQGWEKNADYYEILQQNFER
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHH
DRALNYTFSGPQKADFRFKAQGLPVEDVLSRGQLKLLMCALRLAQGEHLMKEKQRHCIFL
HHCCEEEECCCCCCCCEEEECCCCHHHHHCCCHHHHHHHHHHHHCCHHHHHHCCCCEEEE
IDDFASELDQYKRALLAERLQQSGSQVFVTAITQRQLKEMQVENKKMFSVHNGIINALN
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA