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The map label for this gene is dnaN

Identifier: 16272930

GI number: 16272930

Start: 1054796

End: 1055896

Strand: Reverse

Name: dnaN

Synonym: HI0992

Alternate gene names: 16272930

Gene position: 1055896-1054796 (Counterclockwise)

Preceding gene: 16272931

Following gene: 16272929

Centisome position: 57.69

GC content: 38.24

Gene sequence:

>1101_bases
ATGCAATTTAGTATTTCAAGAGAAAATTTATTAAAACCGTTGCAACAAGTATGTGGCGTATTGAGTAATCGCCCAAATAT
TCCTGTATTAAACAACGTATTATTGCAAATTGAAGACTATCGTCTGACTATCACAGGGACAGATTTAGAAGTTGAACTCT
CTAGTCAAACTCAACTTTCATCATCTTCTGAAAATGGCACTTTTACCATTCCAGCAAAAAAATTCTTAGATATTTGCCGC
ACTTTATCTGATGATTCAGAAATCACGGTGACTTTTGAACAAGATCGTGCATTAGTGCAATCTGGGCGTAGCCGTTTTAC
ACTAGCAACCCAACCTGCCGAAGAATATCCCAACCTCACAGATTGGCAGTCTGAAGTGGATTTTGAACTACCGCAAAATA
CCTTACGCCGTTTAATTGAAGCCACTCAATTTTCTATGGCAAACCAAGATGCCCGCTATTTCTTAAATGGTATGAAGTTT
GAAACAGAAGGCAATTTATTGCGAACTGTGGCAACAGATGGTCATAGACTTGCGGTCTGTACTATCTCACTTGAACAAGA
ATTACAAAATCATTCTGTGATTTTACCTCGTAAAGGCGTGTTAGAACTTGTTCGTTTATTAGAAACAAACGATGAACCAG
CACGCTTACAAATTGGCACCAACAATTTACGTGTCCATTTAAAAAACACAGTATTTACATCAAAATTAATTGATGGTCGC
TTTCCTGACTATCGCCGAGTATTGCCTCGTAATGCCACAAAAATTGTCGAAGGCAATTGGGAAATGCTGAAACAGGCTTT
TGCTCGTGCGTCTATTTTATCTAATGAACGCGCACGCAGCGTACGTTTAAGTTTAAAGGAAAACCAGCTTAAAATTACTG
CCTCAAATACAGAACACGAAGAAGCAGAAGAAATTGTCGATGTCAATTACAATGGCGAAGAATTAGAAGTGGGCTTTAAT
GTGACCTATATTCTTGATGTGCTGAATGCACTTAAATGTAATCAAGTGCGAATGTGCTTAACTGATGCGTTCTCCAGTTG
CTTAATTGAAAACTGTGAAGACAGCAGCTGCGAATACGTCATTATGCCAATGCGTTTATAA

Upstream 100 bases:

>100_bases
CGCTTGCCGAGAAGTACCAAAATTCCGTGAGCAAGACAATAGCATTCAAGAAGATTGGGCGAATTTGATCCGCACTTTGT
CCGCATAAAAGGAGAACGTA

Downstream 100 bases:

>100_bases
TATGGCGATTTCTCGTTTACTGGTCGAAAAATTTCGTAATTTAACAGCAGTGGATCTTGATTTTGATCCCTGCTTTAACT
TTTTAATCGGCAACAACGGC

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MQFSISRENLLKPLQQVCGVLSNRPNIPVLNNVLLQIEDYRLTITGTDLEVELSSQTQLSSSSENGTFTIPAKKFLDICR
TLSDDSEITVTFEQDRALVQSGRSRFTLATQPAEEYPNLTDWQSEVDFELPQNTLRRLIEATQFSMANQDARYFLNGMKF
ETEGNLLRTVATDGHRLAVCTISLEQELQNHSVILPRKGVLELVRLLETNDEPARLQIGTNNLRVHLKNTVFTSKLIDGR
FPDYRRVLPRNATKIVEGNWEMLKQAFARASILSNERARSVRLSLKENQLKITASNTEHEEAEEIVDVNYNGEELEVGFN
VTYILDVLNALKCNQVRMCLTDAFSSCLIENCEDSSCEYVIMPMRL

Sequences:

>Translated_366_residues
MQFSISRENLLKPLQQVCGVLSNRPNIPVLNNVLLQIEDYRLTITGTDLEVELSSQTQLSSSSENGTFTIPAKKFLDICR
TLSDDSEITVTFEQDRALVQSGRSRFTLATQPAEEYPNLTDWQSEVDFELPQNTLRRLIEATQFSMANQDARYFLNGMKF
ETEGNLLRTVATDGHRLAVCTISLEQELQNHSVILPRKGVLELVRLLETNDEPARLQIGTNNLRVHLKNTVFTSKLIDGR
FPDYRRVLPRNATKIVEGNWEMLKQAFARASILSNERARSVRLSLKENQLKITASNTEHEEAEEIVDVNYNGEELEVGFN
VTYILDVLNALKCNQVRMCLTDAFSSCLIENCEDSSCEYVIMPMRL
>Mature_366_residues
MQFSISRENLLKPLQQVCGVLSNRPNIPVLNNVLLQIEDYRLTITGTDLEVELSSQTQLSSSSENGTFTIPAKKFLDICR
TLSDDSEITVTFEQDRALVQSGRSRFTLATQPAEEYPNLTDWQSEVDFELPQNTLRRLIEATQFSMANQDARYFLNGMKF
ETEGNLLRTVATDGHRLAVCTISLEQELQNHSVILPRKGVLELVRLLETNDEPARLQIGTNNLRVHLKNTVFTSKLIDGR
FPDYRRVLPRNATKIVEGNWEMLKQAFARASILSNERARSVRLSLKENQLKITASNTEHEEAEEIVDVNYNGEELEVGFN
VTYILDVLNALKCNQVRMCLTDAFSSCLIENCEDSSCEYVIMPMRL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=366, Percent_Identity=62.568306010929, Blast_Score=499, Evalue=1e-142,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): DPO3B_HAEIN (P43744)

Other databases:

- EMBL:   L42023
- PIR:   A64107
- RefSeq:   NP_439155.1
- ProteinModelPortal:   P43744
- SMR:   P43744
- GeneID:   949992
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0992
- NMPDR:   fig|71421.1.peg.959
- TIGR:   HI_0992
- HOGENOM:   HBG586514
- OMA:   DYNRVIP
- ProtClustDB:   PRK05643
- BioCyc:   HINF71421:HI_0992-MONOMER
- BRENDA:   2.7.7.7
- GO:   GO:0005737
- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634
- Gene3D:   G3DSA:3.10.150.10
- SMART:   SM00480
- TIGRFAMs:   TIGR00663

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3

EC number: =2.7.7.7

Molecular weight: Translated: 41632; Mature: 41632

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFSISRENLLKPLQQVCGVLSNRPNIPVLNNVLLQIEDYRLTITGTDLEVELSSQTQLS
CCCCCCHHHHHHHHHHHHHHHCCCCCCCHHCCEEEEEEEEEEEEECCCEEEEECCCCCCC
SSSENGTFTIPAKKFLDICRTLSDDSEITVTFEQDRALVQSGRSRFTLATQPAEEYPNLT
CCCCCCEEEECHHHHHHHHHHCCCCCEEEEEECCCHHHHHCCCCEEEEEECCHHHCCCCC
DWQSEVDFELPQNTLRRLIEATQFSMANQDARYFLNGMKFETEGNLLRTVATDGHRLAVC
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCEEEECCCCEEEEEECCCCEEEEE
TISLEQELQNHSVILPRKGVLELVRLLETNDEPARLQIGTNNLRVHLKNTVFTSKLIDGR
EEEHHHHHCCCCEECCCHHHHHHHHHHHCCCCCCEEEECCCCEEEEEECCEEHHHHHCCC
FPDYRRVLPRNATKIVEGNWEMLKQAFARASILSNERARSVRLSLKENQLKITASNTEHE
CCHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHCCCCHHEEEEEEECCEEEEEECCCCHH
EAEEIVDVNYNGEELEVGFNVTYILDVLNALKCNQVRMCLTDAFSSCLIENCEDSSCEYV
HHHHEEECCCCCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCEE
IMPMRL
EEECCC
>Mature Secondary Structure
MQFSISRENLLKPLQQVCGVLSNRPNIPVLNNVLLQIEDYRLTITGTDLEVELSSQTQLS
CCCCCCHHHHHHHHHHHHHHHCCCCCCCHHCCEEEEEEEEEEEEECCCEEEEECCCCCCC
SSSENGTFTIPAKKFLDICRTLSDDSEITVTFEQDRALVQSGRSRFTLATQPAEEYPNLT
CCCCCCEEEECHHHHHHHHHHCCCCCEEEEEECCCHHHHHCCCCEEEEEECCHHHCCCCC
DWQSEVDFELPQNTLRRLIEATQFSMANQDARYFLNGMKFETEGNLLRTVATDGHRLAVC
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCEEEECCCCEEEEEECCCCEEEEE
TISLEQELQNHSVILPRKGVLELVRLLETNDEPARLQIGTNNLRVHLKNTVFTSKLIDGR
EEEHHHHHCCCCEECCCHHHHHHHHHHHCCCCCCEEEECCCCEEEEEECCEEHHHHHCCC
FPDYRRVLPRNATKIVEGNWEMLKQAFARASILSNERARSVRLSLKENQLKITASNTEHE
CCHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHCCCCHHEEEEEEECCEEEEEECCCCHH
EAEEIVDVNYNGEELEVGFNVTYILDVLNALKCNQVRMCLTDAFSSCLIENCEDSSCEYV
HHHHEEECCCCCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCEE
IMPMRL
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800