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The map label for this gene is leuB [H]

Identifier: 16272925

GI number: 16272925

Start: 1044988

End: 1046064

Strand: Direct

Name: leuB [H]

Synonym: HI0987

Alternate gene names: 16272925

Gene position: 1044988-1046064 (Clockwise)

Preceding gene: 30995416

Following gene: 16272926

Centisome position: 57.1

GC content: 42.15

Gene sequence:

>1077_bases
ATGCAATCATACAATATAGCAGTATTAGCAGGCGATGGTATTGGTCCCGAAGTCATGGCGGAAGCCATTAAGGTATTAAA
CAGAGTTCAAGAAAAATTTGGTTTTAAACTTAACTTTAACGAGTTTTTTGTCGGCGGCGCGGCTATTGAGCATTGCGGTT
ATCCGTTACCAGCAGAAACCTTAAAAGGCTGTGATCAAGCGGATGCCATTTTATTTGGTTCTGTAGGTGGCCCTAAATGG
ACAAATTTACCACCAGATCAACAGCCAGAACGTGGTGCATTATTGCCTTTGCGTAAACATTTCAAATTATTCTGTAATCT
TCGTCCTGCAACCCTTTATAAAGGCTTGGAAAAATTCTGTCCATTACGTGCAGATATTGCCGCAAAAGGTTTTGATATGG
TGGTGGTGCGTGAATTAACGGGTGGGATTTATTTCGGTCAGCCTAAAGGTCGTGAGGGGGATGGCGTACAAACCAAAGCA
TTTGATACGGAAGTGTATTACAAATATGAAATCGAACGAATTGCACGTGCGGCTTTTGAGGCAGCAATGAAACGCAATAA
AAAAGTCACTTCTGTAGATAAAGCGAACGTATTACAGTCTTCTATTTTATGGCGTGAAACTGTGACAGAAATGGCAAAAG
ACTATCCTGAAGTGACTTTAGAGCATATCTATATCGACAATGCCACCATGCAGTTAATTAAATCACCTGAATCTTTTGAT
GTTTTACTTTGCTCTAACATTTTCGGCGATATTATTTCTGACGAAGCGGCGATGATCACAGGTTCTATGGGAATGTTGCC
ATCTGCCAGCTTAAATGAAGAGGGTTTTGGTTTATACGAGCCTGCAGGCGGTTCTGCCCCTGATATTGCAGGCAAAGGCA
TTGCTAACCCAATCGCACAAATTCTTTCTGCGGCAATGATGTTGCGTTATAGCTTCAACTTAAACGAAGCAGCAGATGCC
ATTGAAAGTGCGGTTCAAAAAGTCTTGGCAAGTGGTCATCGCACCGCCGATTTGGCCGATGATTCCACACCAGTTTCAAC
GGCAGAAATGGGGACATTGATTACACAAGCAATTTAA

Upstream 100 bases:

>100_bases
CATAAACATCATTAATTTATCGAGTTCCCCTTTTTAGCAAAGAGGGGAAAAATACAAAAAAACTAACCGCACTTTTGAAG
TAAAAAATAGGAAAAATAAT

Downstream 100 bases:

>100_bases
TACTTGCCTTCCTCCGTTTACGGGGGAAGGTGCCTGAAGGGCGGAAGGGGGCAATCTCCACCCTTAGTTATTTAGAAATA
TCCCCAATAAACAGGGGGGA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MQSYNIAVLAGDGIGPEVMAEAIKVLNRVQEKFGFKLNFNEFFVGGAAIEHCGYPLPAETLKGCDQADAILFGSVGGPKW
TNLPPDQQPERGALLPLRKHFKLFCNLRPATLYKGLEKFCPLRADIAAKGFDMVVVRELTGGIYFGQPKGREGDGVQTKA
FDTEVYYKYEIERIARAAFEAAMKRNKKVTSVDKANVLQSSILWRETVTEMAKDYPEVTLEHIYIDNATMQLIKSPESFD
VLLCSNIFGDIISDEAAMITGSMGMLPSASLNEEGFGLYEPAGGSAPDIAGKGIANPIAQILSAAMMLRYSFNLNEAADA
IESAVQKVLASGHRTADLADDSTPVSTAEMGTLITQAI

Sequences:

>Translated_358_residues
MQSYNIAVLAGDGIGPEVMAEAIKVLNRVQEKFGFKLNFNEFFVGGAAIEHCGYPLPAETLKGCDQADAILFGSVGGPKW
TNLPPDQQPERGALLPLRKHFKLFCNLRPATLYKGLEKFCPLRADIAAKGFDMVVVRELTGGIYFGQPKGREGDGVQTKA
FDTEVYYKYEIERIARAAFEAAMKRNKKVTSVDKANVLQSSILWRETVTEMAKDYPEVTLEHIYIDNATMQLIKSPESFD
VLLCSNIFGDIISDEAAMITGSMGMLPSASLNEEGFGLYEPAGGSAPDIAGKGIANPIAQILSAAMMLRYSFNLNEAADA
IESAVQKVLASGHRTADLADDSTPVSTAEMGTLITQAI
>Mature_358_residues
MQSYNIAVLAGDGIGPEVMAEAIKVLNRVQEKFGFKLNFNEFFVGGAAIEHCGYPLPAETLKGCDQADAILFGSVGGPKW
TNLPPDQQPERGALLPLRKHFKLFCNLRPATLYKGLEKFCPLRADIAAKGFDMVVVRELTGGIYFGQPKGREGDGVQTKA
FDTEVYYKYEIERIARAAFEAAMKRNKKVTSVDKANVLQSSILWRETVTEMAKDYPEVTLEHIYIDNATMQLIKSPESFD
VLLCSNIFGDIISDEAAMITGSMGMLPSASLNEEGFGLYEPAGGSAPDIAGKGIANPIAQILSAAMMLRYSFNLNEAADA
IESAVQKVLASGHRTADLADDSTPVSTAEMGTLITQAI

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=342, Percent_Identity=31.8713450292398, Blast_Score=141, Evalue=8e-34,
Organism=Homo sapiens, GI28178838, Length=327, Percent_Identity=29.6636085626911, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI4758582, Length=327, Percent_Identity=29.6636085626911, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI28178821, Length=347, Percent_Identity=28.2420749279539, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI28178816, Length=347, Percent_Identity=27.9538904899135, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI28178819, Length=177, Percent_Identity=37.2881355932203, Blast_Score=102, Evalue=5e-22,
Organism=Escherichia coli, GI87081683, Length=357, Percent_Identity=71.4285714285714, Blast_Score=538, Evalue=1e-154,
Organism=Escherichia coli, GI1788101, Length=359, Percent_Identity=38.9972144846797, Blast_Score=222, Evalue=4e-59,
Organism=Escherichia coli, GI1787381, Length=400, Percent_Identity=26.5, Blast_Score=99, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71986051, Length=348, Percent_Identity=31.6091954022989, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17550882, Length=361, Percent_Identity=30.1939058171745, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI25144293, Length=348, Percent_Identity=31.0344827586207, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17505779, Length=353, Percent_Identity=29.1784702549575, Blast_Score=119, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6319830, Length=364, Percent_Identity=47.5274725274725, Blast_Score=293, Evalue=3e-80,
Organism=Saccharomyces cerevisiae, GI6322097, Length=350, Percent_Identity=37.4285714285714, Blast_Score=177, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6324709, Length=354, Percent_Identity=33.0508474576271, Blast_Score=145, Evalue=9e-36,
Organism=Saccharomyces cerevisiae, GI6324291, Length=269, Percent_Identity=33.8289962825279, Blast_Score=132, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24643268, Length=338, Percent_Identity=34.3195266272189, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24643270, Length=338, Percent_Identity=34.3195266272189, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24661184, Length=344, Percent_Identity=32.2674418604651, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI161078635, Length=358, Percent_Identity=28.7709497206704, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI161078633, Length=358, Percent_Identity=28.7709497206704, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24650122, Length=358, Percent_Identity=28.7709497206704, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI161078637, Length=358, Percent_Identity=28.7709497206704, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI161078639, Length=356, Percent_Identity=28.3707865168539, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI281362242, Length=341, Percent_Identity=27.2727272727273, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648872, Length=341, Percent_Identity=27.2727272727273, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI20130355, Length=341, Percent_Identity=24.0469208211144, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38809; Mature: 38809

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSYNIAVLAGDGIGPEVMAEAIKVLNRVQEKFGFKLNFNEFFVGGAAIEHCGYPLPAET
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCEECCHHEEECCHHHHHCCCCCCHHH
LKGCDQADAILFGSVGGPKWTNLPPDQQPERGALLPLRKHFKLFCNLRPATLYKGLEKFC
HCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHC
PLRADIAAKGFDMVVVRELTGGIYFGQPKGREGDGVQTKAFDTEVYYKYEIERIARAAFE
CCHHHHHHCCCCEEEEEEHHCCEEECCCCCCCCCCCEEEEECCEEEEEHHHHHHHHHHHH
AAMKRNKKVTSVDKANVLQSSILWRETVTEMAKDYPEVTLEHIYIDNATMQLIKSPESFD
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHCCCCCCE
VLLCSNIFGDIISDEAAMITGSMGMLPSASLNEEGFGLYEPAGGSAPDIAGKGIANPIAQ
EEEHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHH
ILSAAMMLRYSFNLNEAADAIESAVQKVLASGHRTADLADDSTPVSTAEMGTLITQAI
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MQSYNIAVLAGDGIGPEVMAEAIKVLNRVQEKFGFKLNFNEFFVGGAAIEHCGYPLPAET
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCEECCHHEEECCHHHHHCCCCCCHHH
LKGCDQADAILFGSVGGPKWTNLPPDQQPERGALLPLRKHFKLFCNLRPATLYKGLEKFC
HCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHC
PLRADIAAKGFDMVVVRELTGGIYFGQPKGREGDGVQTKAFDTEVYYKYEIERIARAAFE
CCHHHHHHCCCCEEEEEEHHCCEEECCCCCCCCCCCEEEEECCEEEEEHHHHHHHHHHHH
AAMKRNKKVTSVDKANVLQSSILWRETVTEMAKDYPEVTLEHIYIDNATMQLIKSPESFD
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHCCCCCCE
VLLCSNIFGDIISDEAAMITGSMGMLPSASLNEEGFGLYEPAGGSAPDIAGKGIANPIAQ
EEEHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHH
ILSAAMMLRYSFNLNEAADAIESAVQKVLASGHRTADLADDSTPVSTAEMGTLITQAI
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA