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The map label for this gene is leuA [H]

Identifier: 30995416

GI number: 30995416

Start: 1043355

End: 1044902

Strand: Direct

Name: leuA [H]

Synonym: HI0986

Alternate gene names: 30995416

Gene position: 1043355-1044902 (Clockwise)

Preceding gene: 16272918

Following gene: 16272925

Centisome position: 57.01

GC content: 41.34

Gene sequence:

>1548_bases
ATGACAGATCGCGTTATTATTTTTGATACCACCTTGCGTGATGGGGAGCAGGCATTAAAAGCAAGTTTGACGGTAAAAGA
AAAATTACAGATCGCTTTAGCACTTGAACGCTTAGGTGTGGATGTAATGGAAGTAGGGTTTCCTGTTTCTTCTCAAGGGG
ATTTTGAATCTGTTCAAACCATTGCTCGCCATATTAAAAACGCGCGTGTTGCTGCGTTATCTCGAGCGGTAGATAAAGAC
ATTGATGCCGCATACGAAGCATTGAAAGTGGCAGAAGCATTCCGTATTCATACGTTTATTGCAAGTTCCGCATTGCACGT
TGAAGCGAAATTAAAACGTTCTTTTGATGATGTGGTAGGAATGGCAGTTGCAGCAGTGAAACGCGCACGTAATTATACGG
ATGATGTTGAGTTCTCTTGCGAAGATGCAGGCCGTACAGGCATTGATAATATCTGTCGTATTGTTGAAGCGGCAATTAAC
GCAGGTGCAACTACGGTAAACATTCCAGATACAGTCGGTTTTTGCTTACCAAATGAATACGGCAATATCATTGCTCAAGT
GCGTAATTGCGTGCCGAATATTGATAAAGCAGTGATTTCCGTGCATTGCCACAATGACTTAGGTATGGCAACCGCTAATT
CTTTAACAGCAGTACAAAATGGTGCGCGCCAAATTGAATGTACCATTAATGGAATTGGCGAACGTGCGGGCAATACCTCT
CTTGAAGAAGTGGTTATGGCAATGAAAGTGCGTCAGGATTTTATGGGTGTGGATACCCATATTAATACTCAAGAAATTCA
CCGTGTCAGCCAAATGGTAAGCCAACTTTGCAATATGCCGATTCAGCCGAATAAAGCCATTGTGGGCTCAAATGCTTTTG
CCCATTCTTCTGGCATCCATCAAGATGGTATGTTGAAAAACAAAAATACTTACGAAATTCTGTCTCCAGAAACCATCGGT
TTGAAAAAAGAAAAATTGAATTTAACCGCACGTTCTGGTCGTGCAGCTGTGAAAGGTCATATGGCAGATATGGGCTATAA
CGAACAAGATTACGATTTGGATAAATTGTATGACGAGTTCTTAAAATTGGCGGATAAAAAAGGTCAAGTTTTTGATTATG
ATTTGGAAGCATTGGCGTTTATCGATATGCAACAAGGGGATGAGGATCGTTTGGTATTAGATAAACTTTCGGCACACTCC
ACCAAAGAATATCCAGCAACTGCGTTTGTTCAATTGAAGTTGGATGGCGAAAAATTAAGTACGTCTTCAATTGGCGGTAA
TGGTCCAGTGGATGCGGTTTATAACGCCATCTTAAATTTAACGGGTTTAGAAATCAAAATGTCTCACTATAACTTAACTG
CTAAAGGCGAGGGCGCAGAAGCATTAGGGCAAGTGGATATTGTGGTAGAACACAAAGGGCGTAAATTCCACGGCGTCGGT
TTAGCGACAGACATTGTTGAATCTTCCGCACTTGCTTTAGTCCACGCCATCAATGCCATTTATCGAGCGCATAAAGTGGC
AGATATTAAGAATCATAAACATCATTAA

Upstream 100 bases:

>100_bases
GTTGCGGGTTTTTTATCTAAAAAATACAGGAAAAACGCACCGCACTTTTGGAGTGATTTTAGAGGAACTTTTAGTAAGGA
CATTTAGTAAGGACATTGTT

Downstream 100 bases:

>100_bases
TTTATCGAGTTCCCCTTTTTAGCAAAGAGGGGAAAAATACAAAAAAACTAACCGCACTTTTGAAGTAAAAAATAGGAAAA
ATAATATGCAATCATACAAT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MTDRVIIFDTTLRDGEQALKASLTVKEKLQIALALERLGVDVMEVGFPVSSQGDFESVQTIARHIKNARVAALSRAVDKD
IDAAYEALKVAEAFRIHTFIASSALHVEAKLKRSFDDVVGMAVAAVKRARNYTDDVEFSCEDAGRTGIDNICRIVEAAIN
AGATTVNIPDTVGFCLPNEYGNIIAQVRNCVPNIDKAVISVHCHNDLGMATANSLTAVQNGARQIECTINGIGERAGNTS
LEEVVMAMKVRQDFMGVDTHINTQEIHRVSQMVSQLCNMPIQPNKAIVGSNAFAHSSGIHQDGMLKNKNTYEILSPETIG
LKKEKLNLTARSGRAAVKGHMADMGYNEQDYDLDKLYDEFLKLADKKGQVFDYDLEALAFIDMQQGDEDRLVLDKLSAHS
TKEYPATAFVQLKLDGEKLSTSSIGGNGPVDAVYNAILNLTGLEIKMSHYNLTAKGEGAEALGQVDIVVEHKGRKFHGVG
LATDIVESSALALVHAINAIYRAHKVADIKNHKHH

Sequences:

>Translated_515_residues
MTDRVIIFDTTLRDGEQALKASLTVKEKLQIALALERLGVDVMEVGFPVSSQGDFESVQTIARHIKNARVAALSRAVDKD
IDAAYEALKVAEAFRIHTFIASSALHVEAKLKRSFDDVVGMAVAAVKRARNYTDDVEFSCEDAGRTGIDNICRIVEAAIN
AGATTVNIPDTVGFCLPNEYGNIIAQVRNCVPNIDKAVISVHCHNDLGMATANSLTAVQNGARQIECTINGIGERAGNTS
LEEVVMAMKVRQDFMGVDTHINTQEIHRVSQMVSQLCNMPIQPNKAIVGSNAFAHSSGIHQDGMLKNKNTYEILSPETIG
LKKEKLNLTARSGRAAVKGHMADMGYNEQDYDLDKLYDEFLKLADKKGQVFDYDLEALAFIDMQQGDEDRLVLDKLSAHS
TKEYPATAFVQLKLDGEKLSTSSIGGNGPVDAVYNAILNLTGLEIKMSHYNLTAKGEGAEALGQVDIVVEHKGRKFHGVG
LATDIVESSALALVHAINAIYRAHKVADIKNHKHH
>Mature_514_residues
TDRVIIFDTTLRDGEQALKASLTVKEKLQIALALERLGVDVMEVGFPVSSQGDFESVQTIARHIKNARVAALSRAVDKDI
DAAYEALKVAEAFRIHTFIASSALHVEAKLKRSFDDVVGMAVAAVKRARNYTDDVEFSCEDAGRTGIDNICRIVEAAINA
GATTVNIPDTVGFCLPNEYGNIIAQVRNCVPNIDKAVISVHCHNDLGMATANSLTAVQNGARQIECTINGIGERAGNTSL
EEVVMAMKVRQDFMGVDTHINTQEIHRVSQMVSQLCNMPIQPNKAIVGSNAFAHSSGIHQDGMLKNKNTYEILSPETIGL
KKEKLNLTARSGRAAVKGHMADMGYNEQDYDLDKLYDEFLKLADKKGQVFDYDLEALAFIDMQQGDEDRLVLDKLSAHST
KEYPATAFVQLKLDGEKLSTSSIGGNGPVDAVYNAILNLTGLEIKMSHYNLTAKGEGAEALGQVDIVVEHKGRKFHGVGL
ATDIVESSALALVHAINAIYRAHKVADIKNHKHH

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI62198232, Length=297, Percent_Identity=23.5690235690236, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1786261, Length=506, Percent_Identity=67.1936758893281, Blast_Score=692, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320019, Length=360, Percent_Identity=28.6111111111111, Blast_Score=139, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6320071, Length=373, Percent_Identity=28.1501340482574, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6324225, Length=560, Percent_Identity=25.3571428571429, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6324682, Length=537, Percent_Identity=25.3258845437616, Blast_Score=132, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24582381, Length=289, Percent_Identity=23.8754325259516, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 56081; Mature: 55950

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDRVIIFDTTLRDGEQALKASLTVKEKLQIALALERLGVDVMEVGFPVSSQGDFESVQT
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHH
IARHIKNARVAALSRAVDKDIDAAYEALKVAEAFRIHTFIASSALHVEAKLKRSFDDVVG
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
MAVAAVKRARNYTDDVEFSCEDAGRTGIDNICRIVEAAINAGATTVNIPDTVGFCLPNEY
HHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHH
GNIIAQVRNCVPNIDKAVISVHCHNDLGMATANSLTAVQNGARQIECTINGIGERAGNTS
HHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHCCCEEEEEEEECCCCCCCCCC
LEEVVMAMKVRQDFMGVDTHINTQEIHRVSQMVSQLCNMPIQPNKAIVGSNAFAHSSGIH
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCC
QDGMLKNKNTYEILSPETIGLKKEKLNLTARSGRAAVKGHMADMGYNEQDYDLDKLYDEF
CCCCCCCCCCEEEECCCCCCCCHHHEEEEECCCCHHHHHHHHHCCCCCCCCCHHHHHHHH
LKLADKKGQVFDYDLEALAFIDMQQGDEDRLVLDKLSAHSTKEYPATAFVQLKLDGEKLS
HHHHCCCCCEEEECCCEEEEEECCCCCCCHHHHHHHHCCCCCCCCCEEEEEEEECCCCCC
TSSIGGNGPVDAVYNAILNLTGLEIKMSHYNLTAKGEGAEALGQVDIVVEHKGRKFHGVG
CCCCCCCCCHHHHHHHHHHCCCEEEEEEEEEEEECCCCCHHHCCEEEEEECCCCEEECCC
LATDIVESSALALVHAINAIYRAHKVADIKNHKHH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TDRVIIFDTTLRDGEQALKASLTVKEKLQIALALERLGVDVMEVGFPVSSQGDFESVQT
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHH
IARHIKNARVAALSRAVDKDIDAAYEALKVAEAFRIHTFIASSALHVEAKLKRSFDDVVG
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
MAVAAVKRARNYTDDVEFSCEDAGRTGIDNICRIVEAAINAGATTVNIPDTVGFCLPNEY
HHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHH
GNIIAQVRNCVPNIDKAVISVHCHNDLGMATANSLTAVQNGARQIECTINGIGERAGNTS
HHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHCCCEEEEEEEECCCCCCCCCC
LEEVVMAMKVRQDFMGVDTHINTQEIHRVSQMVSQLCNMPIQPNKAIVGSNAFAHSSGIH
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCC
QDGMLKNKNTYEILSPETIGLKKEKLNLTARSGRAAVKGHMADMGYNEQDYDLDKLYDEF
CCCCCCCCCCEEEECCCCCCCCHHHEEEEECCCCHHHHHHHHHCCCCCCCCCHHHHHHHH
LKLADKKGQVFDYDLEALAFIDMQQGDEDRLVLDKLSAHSTKEYPATAFVQLKLDGEKLS
HHHHCCCCCEEEECCCEEEEEECCCCCCCHHHHHHHHCCCCCCCCCEEEEEEEECCCCCC
TSSIGGNGPVDAVYNAILNLTGLEIKMSHYNLTAKGEGAEALGQVDIVVEHKGRKFHGVG
CCCCCCCCCHHHHHHHHHHCCCEEEEEEEEEEEECCCCCHHHCCEEEEEECCCCEEECCC
LATDIVESSALALVHAINAIYRAHKVADIKNHKHH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA