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The map label for this gene is smpB

Identifier: 16272919

GI number: 16272919

Start: 1038488

End: 1038973

Strand: Reverse

Name: smpB

Synonym: HI0981

Alternate gene names: 16272919

Gene position: 1038973-1038488 (Counterclockwise)

Preceding gene: 16272920

Following gene: 16272903

Centisome position: 56.77

GC content: 35.6

Gene sequence:

>486_bases
ATGACAAAGAAAAAAGTAAAACCCAATTCAAATACTATCGCACTAAATAAACGTGCAAGACACGATTATTTTATTGAAGA
TGAAATTGAAGCAGGTCTTGAATTACAAGGCTGGGAAGTCAAATCTATGCGCGCAGGCAAGGCAAATATTAGTGATAGTT
ATGTTATTTTTAAAAATGGCGAAGCCTTTTTATTCGGCGCAAGCATTCAGCCATTAAATGTTGCATCAACGCATATTGTT
TGTGATCCAACTCGCACTCGTAAGTTATTGTTAAATAAACGCGAATTAGCATCCCTATTTGGTAAAGCAAACCGAGACGG
TTTTACCATAGTTGCACTTTCTCTTTATTGGAAAAGTGCGTGGGCAAAAGTCAAAATCGGTTTAGCCAAAGGTAAAAAAC
AACAGGATAAACGTGATGATATTAAAGAACGTGAATGGAAAGTAACAAAAGATCGCATTATGAAAAATGCACATCGAAGA
TCTTAA

Upstream 100 bases:

>100_bases
GATTGAAAATAAATGCGACTGCCAGTTCTACCTAGATGATCTTTTTGCTATCAAAATAGAGATCCGATAAAATGCCTCAG
GCAAAATAAGGGATAAAATT

Downstream 100 bases:

>100_bases
ATTGTAATAATAAATTAGCCCCAAATTACTTGGGGCTAATTTTTAAAATAGAATGAAAAACATTCAATCTTTTTGTTAAT
AAAAGAATATCGCCTTATCC

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 161; Mature: 160

Protein sequence:

>161_residues
MTKKKVKPNSNTIALNKRARHDYFIEDEIEAGLELQGWEVKSMRAGKANISDSYVIFKNGEAFLFGASIQPLNVASTHIV
CDPTRTRKLLLNKRELASLFGKANRDGFTIVALSLYWKSAWAKVKIGLAKGKKQQDKRDDIKEREWKVTKDRIMKNAHRR
S

Sequences:

>Translated_161_residues
MTKKKVKPNSNTIALNKRARHDYFIEDEIEAGLELQGWEVKSMRAGKANISDSYVIFKNGEAFLFGASIQPLNVASTHIV
CDPTRTRKLLLNKRELASLFGKANRDGFTIVALSLYWKSAWAKVKIGLAKGKKQQDKRDDIKEREWKVTKDRIMKNAHRR
S
>Mature_160_residues
TKKKVKPNSNTIALNKRARHDYFIEDEIEAGLELQGWEVKSMRAGKANISDSYVIFKNGEAFLFGASIQPLNVASTHIVC
DPTRTRKLLLNKRELASLFGKANRDGFTIVALSLYWKSAWAKVKIGLAKGKKQQDKRDDIKEREWKVTKDRIMKNAHRRS

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=160, Percent_Identity=78.75, Blast_Score=267, Evalue=3e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_HAEI8 (Q4QLS9)

Other databases:

- EMBL:   CP000057
- RefSeq:   YP_248678.1
- ProteinModelPortal:   Q4QLS9
- SMR:   Q4QLS9
- STRING:   Q4QLS9
- GeneID:   3430443
- GenomeReviews:   CP000057_GR
- KEGG:   hit:NTHI1153
- eggNOG:   COG0691
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- PhylomeDB:   Q4QLS9
- ProtClustDB:   PRK05422
- BioCyc:   HINF281310:NTHI1153-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 18434; Mature: 18302

Theoretical pI: Translated: 10.82; Mature: 10.82

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKKVKPNSNTIALNKRARHDYFIEDEIEAGLELQGWEVKSMRAGKANISDSYVIFKNG
CCCCCCCCCCCEEEEECCCCCCCEECCHHCCCCEECCEEEECCCCCCCCCCCCEEEEECC
EAFLFGASIQPLNVASTHIVCDPTRTRKLLLNKRELASLFGKANRDGFTIVALSLYWKSA
CEEEEECCCCCCCCCCEEEEECCCHHHHHHHCHHHHHHHHCCCCCCCEEEEEEEEEHHCC
WAKVKIGLAKGKKQQDKRDDIKEREWKVTKDRIMKNAHRRS
CEEEEEEECCCCCCCCHHCCHHHHHCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
TKKKVKPNSNTIALNKRARHDYFIEDEIEAGLELQGWEVKSMRAGKANISDSYVIFKNG
CCCCCCCCCCEEEEECCCCCCCEECCHHCCCCEECCEEEECCCCCCCCCCCCEEEEECC
EAFLFGASIQPLNVASTHIVCDPTRTRKLLLNKRELASLFGKANRDGFTIVALSLYWKSA
CEEEEECCCCCCCCCCEEEEECCCHHHHHHHCHHHHHHHHCCCCCCCEEEEEEEEEHHCC
WAKVKIGLAKGKKQQDKRDDIKEREWKVTKDRIMKNAHRRS
CEEEEEEECCCCCCCCHHCCHHHHHCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA