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The map label for this gene is pfkA [H]

Identifier: 16272920

GI number: 16272920

Start: 1039212

End: 1040177

Strand: Reverse

Name: pfkA [H]

Synonym: HI0982

Alternate gene names: 16272920

Gene position: 1040177-1039212 (Counterclockwise)

Preceding gene: 16272921

Following gene: 16272919

Centisome position: 56.84

GC content: 40.58

Gene sequence:

>966_bases
ATGATTAAAAAAATTGCAGTTTTAACAAGTGGTGGCGATGCGCCTGGTATGAATGCCGCAATTCGTGGCGTAGTACGTTC
TGCACTTGCAGAAGGCTTAGAAGTATTTGGTATCTATGACGGTTATCAAGGTTTATACAATAATAAAATCAAACAATTAA
ATCGCTATAGCGTATCTGATGTGATTAATCGTGGCGGTACTTTTTTAGGTTCTGCACGTTTTCCTGAATTTAAAGATCCT
AATATTCGGGCAAAATGTGCGGAAATTTTACGTTCTCACGGCATTGATGCACTTGTGGTTATCGGTGGTGACGGTTCTTA
CATGGGGGCAAAATTATTAACCGAAGAACACAGTTTCCCTTGTGTTGGCTTACCCGGTACTATTGATAATGATGTTGCAG
GTACTGACTATACCATCGGTTATCAAACCGCATTACAAACTGCAGTGGATGCAATCGACCGTTTACGTGATACATCAAGC
TCACACCAACGTATTTCTATCGTTGAAATTATGGGTCGCCATTGTAGTGACTTAACCATTTCTGCGGGCATTGCTGGCGG
TTGTGAATATATCGTCGCATCAGAAATTGAATTTAATCGTGAAGAATTAATTCAACAAATTGAACGTAGCATTATTCGCG
GTAAACGCCACGCTATTATTGCTATCACTGAACTTTTAACCGATGTTCATTCTCTGGCAAAAGAAATTGAAGCTCGCGTT
GGACACGAAACTCGTGCAACTGTTTTAGGGCATATTCAACGTGGTGGTTCTCCTTGTGCTTTTGACCGTATTCTTGCCTC
ACGTATGGGCGCATACGCAGTAGATTTATTGCTACAAGGCAAAGGCGGCTATTGCGTAGGTATCCAAAATGAACAACTCG
TTCATCACGATATTATTGATGCCATTAATAATATGCAGCGTGTATTTAAGGCTGATTGGCTTAAAGTGGCAAAACGTTTG
GAATAA

Upstream 100 bases:

>100_bases
AATAATGTAGATCAAAATCCCGTCAAATTTTGCTAGAGCAGATTCTTTGAATGATGTAATCTTATGCCCATTTTCAATTA
TGGTTAAAAGTAGGTAAATT

Downstream 100 bases:

>100_bases
TCCTTTATTCACTATATAAATCGCTAACACTTAATTCATCTTTTTATCTTTCCCAAACAGACACAACAATGTGTCTGTTT
TTTCTTAAAAAGTGCGGTCA

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MIKKIAVLTSGGDAPGMNAAIRGVVRSALAEGLEVFGIYDGYQGLYNNKIKQLNRYSVSDVINRGGTFLGSARFPEFKDP
NIRAKCAEILRSHGIDALVVIGGDGSYMGAKLLTEEHSFPCVGLPGTIDNDVAGTDYTIGYQTALQTAVDAIDRLRDTSS
SHQRISIVEIMGRHCSDLTISAGIAGGCEYIVASEIEFNREELIQQIERSIIRGKRHAIIAITELLTDVHSLAKEIEARV
GHETRATVLGHIQRGGSPCAFDRILASRMGAYAVDLLLQGKGGYCVGIQNEQLVHHDIIDAINNMQRVFKADWLKVAKRL
E

Sequences:

>Translated_321_residues
MIKKIAVLTSGGDAPGMNAAIRGVVRSALAEGLEVFGIYDGYQGLYNNKIKQLNRYSVSDVINRGGTFLGSARFPEFKDP
NIRAKCAEILRSHGIDALVVIGGDGSYMGAKLLTEEHSFPCVGLPGTIDNDVAGTDYTIGYQTALQTAVDAIDRLRDTSS
SHQRISIVEIMGRHCSDLTISAGIAGGCEYIVASEIEFNREELIQQIERSIIRGKRHAIIAITELLTDVHSLAKEIEARV
GHETRATVLGHIQRGGSPCAFDRILASRMGAYAVDLLLQGKGGYCVGIQNEQLVHHDIIDAINNMQRVFKADWLKVAKRL
E
>Mature_321_residues
MIKKIAVLTSGGDAPGMNAAIRGVVRSALAEGLEVFGIYDGYQGLYNNKIKQLNRYSVSDVINRGGTFLGSARFPEFKDP
NIRAKCAEILRSHGIDALVVIGGDGSYMGAKLLTEEHSFPCVGLPGTIDNDVAGTDYTIGYQTALQTAVDAIDRLRDTSS
SHQRISIVEIMGRHCSDLTISAGIAGGCEYIVASEIEFNREELIQQIERSIIRGKRHAIIAITELLTDVHSLAKEIEARV
GHETRATVLGHIQRGGSPCAFDRILASRMGAYAVDLLLQGKGGYCVGIQNEQLVHHDIIDAINNMQRVFKADWLKVAKRL
E

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI266453619, Length=346, Percent_Identity=38.4393063583815, Blast_Score=210, Evalue=2e-54,
Organism=Homo sapiens, GI266453768, Length=346, Percent_Identity=38.4393063583815, Blast_Score=210, Evalue=2e-54,
Organism=Homo sapiens, GI266453748, Length=346, Percent_Identity=38.4393063583815, Blast_Score=210, Evalue=2e-54,
Organism=Homo sapiens, GI4505749, Length=346, Percent_Identity=38.4393063583815, Blast_Score=210, Evalue=2e-54,
Organism=Homo sapiens, GI11321601, Length=346, Percent_Identity=37.8612716763006, Blast_Score=209, Evalue=4e-54,
Organism=Homo sapiens, GI48762920, Length=346, Percent_Identity=39.5953757225434, Blast_Score=206, Evalue=3e-53,
Organism=Escherichia coli, GI1790350, Length=320, Percent_Identity=74.375, Blast_Score=490, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI25147584, Length=352, Percent_Identity=36.3636363636364, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17558788, Length=309, Percent_Identity=39.4822006472492, Blast_Score=197, Evalue=6e-51,
Organism=Caenorhabditis elegans, GI72003601, Length=291, Percent_Identity=32.3024054982818, Blast_Score=148, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6321679, Length=349, Percent_Identity=37.2492836676218, Blast_Score=201, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6323861, Length=361, Percent_Identity=35.4570637119114, Blast_Score=187, Evalue=2e-48,
Organism=Drosophila melanogaster, GI28573326, Length=346, Percent_Identity=40.1734104046243, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24652337, Length=346, Percent_Identity=39.5953757225434, Blast_Score=216, Evalue=2e-56,
Organism=Drosophila melanogaster, GI17647809, Length=346, Percent_Identity=39.5953757225434, Blast_Score=216, Evalue=2e-56,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 34922; Mature: 34922

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKIAVLTSGGDAPGMNAAIRGVVRSALAEGLEVFGIYDGYQGLYNNKIKQLNRYSVSD
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCHHEEEEECCHHHHHHHHHHHHHHHHHHH
VINRGGTFLGSARFPEFKDPNIRAKCAEILRSHGIDALVVIGGDGSYMGAKLLTEEHSFP
HHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCC
CVGLPGTIDNDVAGTDYTIGYQTALQTAVDAIDRLRDTSSSHQRISIVEIMGRHCSDLTI
EECCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHCCCCCCCEE
SAGIAGGCEYIVASEIEFNREELIQQIERSIIRGKRHAIIAITELLTDVHSLAKEIEARV
ECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GHETRATVLGHIQRGGSPCAFDRILASRMGAYAVDLLLQGKGGYCVGIQNEQLVHHDIID
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEEEEECCCCEEEECCCCCHHHHHHHH
AINNMQRVFKADWLKVAKRLE
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIKKIAVLTSGGDAPGMNAAIRGVVRSALAEGLEVFGIYDGYQGLYNNKIKQLNRYSVSD
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCHHEEEEECCHHHHHHHHHHHHHHHHHHH
VINRGGTFLGSARFPEFKDPNIRAKCAEILRSHGIDALVVIGGDGSYMGAKLLTEEHSFP
HHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCC
CVGLPGTIDNDVAGTDYTIGYQTALQTAVDAIDRLRDTSSSHQRISIVEIMGRHCSDLTI
EECCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHCCCCCCCEE
SAGIAGGCEYIVASEIEFNREELIQQIERSIIRGKRHAIIAITELLTDVHSLAKEIEARV
ECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GHETRATVLGHIQRGGSPCAFDRILASRMGAYAVDLLLQGKGGYCVGIQNEQLVHHDIID
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEEEEECCCCEEEECCCCCHHHHHHHH
AINNMQRVFKADWLKVAKRLE
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA