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The map label for this gene is dsbE

Identifier: 16272872

GI number: 16272872

Start: 993708

End: 994238

Strand: Reverse

Name: dsbE

Synonym: HI0935

Alternate gene names: 16272872

Gene position: 994238-993708 (Counterclockwise)

Preceding gene: 16272873

Following gene: 16272871

Centisome position: 54.33

GC content: 37.66

Gene sequence:

>531_bases
ATGAACAAAAAACTTATCTTTTTTACACCGCTCTTTGTGCTTTTAGGCGTGTGTATATTATTAATTGCAGGGTTAAATCA
AGATCCGAAAAAAATTGCGTCGGCTTTAATTGATAAACCTGTTCCAGAGTTTTATCAGGCTAATTTACATGAGCCATCAC
AAATCGTAAGCCCGAAAGAGTTTCCTAAACAGCCATTTTTATTAAATGTTTGGGGAAGTTGGTGTGGTTATTGCAAAGAA
GAACATCCTCTGTTAATTGAGATTGCAAAAACGTTGCCTATTGTGGGCGTGGATTATCGCGATAATCGGCAAAATGGCAT
TAAAATGCTTAAGCGAATGGGCAATCCGTTCATCTTAACCATTGATGATAGTCATGGGGAATTGGGATTAAAATTAGGCG
TGGATGGCGCGCCAGAAACTTATCTTGTGGATGAAAATGGTGTGATTCGTTATCGCCATTCTGGTTTATTGGATAAAGAA
ACTTGGCAAACGGTATTTTTGCCGAAAATTGAAGCATTGAAGAACAAATGA

Upstream 100 bases:

>100_bases
ATTATAAACCACTGATTCGTTGGCTTTGGGCGGGCGGTATTTTAATGGCCTTTGGCGCACTTTTTAGCGTTTTCGGATTA
CGCAGAAAACAGGAAAAATA

Downstream 100 bases:

>100_bases
AAAGTATATTAGATATTTTTACTCGCGGTTTTATTCAGGTTCAAAAAACAGTCTTTTTTGCAACCGCACTTTTATTCGCA
TTTTCCTTGTTTACTCAAGC

Product: thiol-disulfide interchange protein

Products: NA

Alternate protein names: Cytochrome c biogenesis protein NrfX

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MNKKLIFFTPLFVLLGVCILLIAGLNQDPKKIASALIDKPVPEFYQANLHEPSQIVSPKEFPKQPFLLNVWGSWCGYCKE
EHPLLIEIAKTLPIVGVDYRDNRQNGIKMLKRMGNPFILTIDDSHGELGLKLGVDGAPETYLVDENGVIRYRHSGLLDKE
TWQTVFLPKIEALKNK

Sequences:

>Translated_176_residues
MNKKLIFFTPLFVLLGVCILLIAGLNQDPKKIASALIDKPVPEFYQANLHEPSQIVSPKEFPKQPFLLNVWGSWCGYCKE
EHPLLIEIAKTLPIVGVDYRDNRQNGIKMLKRMGNPFILTIDDSHGELGLKLGVDGAPETYLVDENGVIRYRHSGLLDKE
TWQTVFLPKIEALKNK
>Mature_176_residues
MNKKLIFFTPLFVLLGVCILLIAGLNQDPKKIASALIDKPVPEFYQANLHEPSQIVSPKEFPKQPFLLNVWGSWCGYCKE
EHPLLIEIAKTLPIVGVDYRDNRQNGIKMLKRMGNPFILTIDDSHGELGLKLGVDGAPETYLVDENGVIRYRHSGLLDKE
TWQTVFLPKIEALKNK

Specific function: Could be involved in disulfide bond formation. Could catalyzes a late, reductive step in the assembly of periplasmic NrfA c-type cytochrome, probably the reduction of disulfide bonds of the apocytochrome c to allow covalent linkage with the heme. Possible

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein; Periplasmic side (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain

Homologues:

Organism=Escherichia coli, GI1788523, Length=176, Percent_Identity=41.4772727272727, Blast_Score=143, Evalue=8e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NRFX_HAEIN (P44943)

Other databases:

- EMBL:   L42023
- PIR:   I64161
- RefSeq:   NP_439095.1
- ProteinModelPortal:   P44943
- SMR:   P44943
- GeneID:   949930
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0935
- NMPDR:   fig|71421.1.peg.901
- TIGR:   HI_0935
- HOGENOM:   HBG493509
- OMA:   TYLVDEN
- ProtClustDB:   CLSK869940
- BioCyc:   HINF71421:HI_0935-MONOMER
- InterPro:   IPR004799
- InterPro:   IPR013740
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335
- Gene3D:   G3DSA:3.40.30.10
- TIGRFAMs:   TIGR00385

Pfam domain/function: PF08534 Redoxin; SSF52833 Thiordxn-like_fd

EC number: NA

Molecular weight: Translated: 19927; Mature: 19927

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS00194 THIOREDOXIN_1; PS51352 THIOREDOXIN_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xce09274)-;

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKLIFFTPLFVLLGVCILLIAGLNQDPKKIASALIDKPVPEFYQANLHEPSQIVSPKE
CCCCEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHCCCCCCHHHCCCHH
FPKQPFLLNVWGSWCGYCKEEHPLLIEIAKTLPIVGVDYRDNRQNGIKMLKRMGNPFILT
CCCCCEEEEECHHHHCCCCCCCCEEEEEHHHCCEEECCCCCCHHHHHHHHHHCCCCEEEE
IDDSHGELGLKLGVDGAPETYLVDENGVIRYRHSGLLDKETWQTVFLPKIEALKNK
EECCCCCEEEEECCCCCCCEEEECCCCEEEEECCCCCCCCCHHEEECCHHHHHCCH
>Mature Secondary Structure
MNKKLIFFTPLFVLLGVCILLIAGLNQDPKKIASALIDKPVPEFYQANLHEPSQIVSPKE
CCCCEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHCCCCCCHHHCCCHH
FPKQPFLLNVWGSWCGYCKEEHPLLIEIAKTLPIVGVDYRDNRQNGIKMLKRMGNPFILT
CCCCCEEEEECHHHHCCCCCCCCEEEEEHHHCCEEECCCCCCHHHHHHHHHHCCCCEEEE
IDDSHGELGLKLGVDGAPETYLVDENGVIRYRHSGLLDKETWQTVFLPKIEALKNK
EECCCCCEEEEECCCCCCCEEEECCCCEEEEECCCCCCCCCHHEEECCHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800