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The map label for this gene is holA

Identifier: 16272860

GI number: 16272860

Start: 980328

End: 981362

Strand: Direct

Name: holA

Synonym: HI0923

Alternate gene names: 16272860

Gene position: 980328-981362 (Clockwise)

Preceding gene: 30995409

Following gene: 16272865

Centisome position: 53.57

GC content: 36.33

Gene sequence:

>1035_bases
ATGAACCGCATTTTTCCAGAGCAACTAAATCATCATCTTGCGCAAGGCTTGGCTAGAGTTTATCTCTTGCAAGGGCAAGA
TCCTTTATTACTTAGTGAAACTGAGGATACTATTTGTCAAGTGGCAAACCTGCAAGGTTTTGATGAAAAAAATACTATTC
AGGTTGATAGCCAAACTGATTGGGCGCAACTTATAGAATCTTGTCAATCTATAGGATTGTTTTTTAGTAAACAAATACTA
AGCTTGAATTTACCTGAAAATTTCACCGCACTTTTGCAGAAAAATCTGCAAGAACTTATATCCGTATTGCATAAAGATGT
CTTGCTTATTTTACAGGTAGCAAAATTGGCTAAAGGGATTGAAAAGCAAACGTGGTTTATTACACTTAATCAATATGAAC
CAAATACAATACTGATAAATTGTCAAACACCGACGGTAGAAAACTTACCACGTTGGGTAAAAAATCGTACCAAAGCAATG
GGATTAGACGCTGACAACGAAGCAATCCAACAACTTTGTTATAGTTATGAAAATAACCTGCTCGCACTCAAACAAGCTCT
GCAGCTTTTAGATTTGCTCTATCCTGACCATAAACTTAACTACAATCGTGTTATTAGCGTGGTGGAACAATCCTCAATTT
TTACACCATTTCAATGGATTGATGCGTTGCTTGTTGGAAAGGCAAATCGTGCTAAACGTATTTTAAAAGGTTTACAAGCT
GAAGATGTGCAACCTGTTATTTTATTACGCACACTACAGCGAGAATTATTTACGTTGCTTGAACTCACAAAACCACAGCA
ACGTATTGTGACAACGGAAAAACTTCCAATTCAACAAATTAAGACTGAATTTGACCGTCTAAAAATCTGGCAAAATCGCC
GACCGCTCTTTTTAAGTGCGATTCAACGTTTAACTTACCAGACTCTCTACGAAATTATCCAAGAACTCGCTAATATTGAA
CGCCTTGCTAAACAAGAATTTAGTGATGAAGTGTGGATCAAACTCGCTGATTTATCTGTAAAAATTTGTTTGTAG

Upstream 100 bases:

>100_bases
CAGAAGAACGAGAGGTAATTTGGAATGATATGCGCGAGCAAGTAGCGCGTCAATTAATCGTAAAAATAATTGCGTTACAA
AATCAAATAAAAAGGAAATA

Downstream 100 bases:

>100_bases
TCACAAGGCAAATAAAATAGACAATATTGAAATAAGATTTTTGATAAAAAAGAAATTTTTTTGCTGGAAAAATGGTCCCC
CCTACCGGACTTGAACCAGT

Product: DNA polymerase III subunit delta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MNRIFPEQLNHHLAQGLARVYLLQGQDPLLLSETEDTICQVANLQGFDEKNTIQVDSQTDWAQLIESCQSIGLFFSKQIL
SLNLPENFTALLQKNLQELISVLHKDVLLILQVAKLAKGIEKQTWFITLNQYEPNTILINCQTPTVENLPRWVKNRTKAM
GLDADNEAIQQLCYSYENNLLALKQALQLLDLLYPDHKLNYNRVISVVEQSSIFTPFQWIDALLVGKANRAKRILKGLQA
EDVQPVILLRTLQRELFTLLELTKPQQRIVTTEKLPIQQIKTEFDRLKIWQNRRPLFLSAIQRLTYQTLYEIIQELANIE
RLAKQEFSDEVWIKLADLSVKICL

Sequences:

>Translated_344_residues
MNRIFPEQLNHHLAQGLARVYLLQGQDPLLLSETEDTICQVANLQGFDEKNTIQVDSQTDWAQLIESCQSIGLFFSKQIL
SLNLPENFTALLQKNLQELISVLHKDVLLILQVAKLAKGIEKQTWFITLNQYEPNTILINCQTPTVENLPRWVKNRTKAM
GLDADNEAIQQLCYSYENNLLALKQALQLLDLLYPDHKLNYNRVISVVEQSSIFTPFQWIDALLVGKANRAKRILKGLQA
EDVQPVILLRTLQRELFTLLELTKPQQRIVTTEKLPIQQIKTEFDRLKIWQNRRPLFLSAIQRLTYQTLYEIIQELANIE
RLAKQEFSDEVWIKLADLSVKICL
>Mature_344_residues
MNRIFPEQLNHHLAQGLARVYLLQGQDPLLLSETEDTICQVANLQGFDEKNTIQVDSQTDWAQLIESCQSIGLFFSKQIL
SLNLPENFTALLQKNLQELISVLHKDVLLILQVAKLAKGIEKQTWFITLNQYEPNTILINCQTPTVENLPRWVKNRTKAM
GLDADNEAIQQLCYSYENNLLALKQALQLLDLLYPDHKLNYNRVISVVEQSSIFTPFQWIDALLVGKANRAKRILKGLQA
EDVQPVILLRTLQRELFTLLELTKPQQRIVTTEKLPIQQIKTEFDRLKIWQNRRPLFLSAIQRLTYQTLYEIIQELANIE
RLAKQEFSDEVWIKLADLSVKICL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The delta subunit seems to interact with the gamma subunit to transfer the b

COG id: COG1466

COG function: function code L; DNA polymerase III, delta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786859, Length=344, Percent_Identity=44.1860465116279, Blast_Score=259, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HOLA_HAEIN (P43747)

Other databases:

- EMBL:   L42023
- PIR:   A64103
- RefSeq:   NP_439083.1
- ProteinModelPortal:   P43747
- SMR:   P43747
- GeneID:   949921
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0923
- NMPDR:   fig|71421.1.peg.889
- TIGR:   HI_0923
- HOGENOM:   HBG739424
- OMA:   FGDDPWL
- ProtClustDB:   PRK05574
- BioCyc:   HINF71421:HI_0923-MONOMER
- BRENDA:   2.7.7.7
- InterPro:   IPR008921
- InterPro:   IPR010372
- InterPro:   IPR005790
- TIGRFAMs:   TIGR01128

Pfam domain/function: PF06144 DNA_pol3_delta; SSF48019 Pol_clamp_load_C

EC number: =2.7.7.7

Molecular weight: Translated: 39846; Mature: 39846

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRIFPEQLNHHLAQGLARVYLLQGQDPLLLSETEDTICQVANLQGFDEKNTIQVDSQTD
CCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHCCCCCCCCCCEEECCCCH
WAQLIESCQSIGLFFSKQILSLNLPENFTALLQKNLQELISVLHKDVLLILQVAKLAKGI
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EKQTWFITLNQYEPNTILINCQTPTVENLPRWVKNRTKAMGLDADNEAIQQLCYSYENNL
CCCEEEEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCH
LALKQALQLLDLLYPDHKLNYNRVISVVEQSSIFTPFQWIDALLVGKANRAKRILKGLQA
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHCCCC
EDVQPVILLRTLQRELFTLLELTKPQQRIVTTEKLPIQQIKTEFDRLKIWQNRRPLFLSA
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHH
IQRLTYQTLYEIIQELANIERLAKQEFSDEVWIKLADLSVKICL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEECCEEEEEC
>Mature Secondary Structure
MNRIFPEQLNHHLAQGLARVYLLQGQDPLLLSETEDTICQVANLQGFDEKNTIQVDSQTD
CCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHCCCCCCCCCCEEECCCCH
WAQLIESCQSIGLFFSKQILSLNLPENFTALLQKNLQELISVLHKDVLLILQVAKLAKGI
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EKQTWFITLNQYEPNTILINCQTPTVENLPRWVKNRTKAMGLDADNEAIQQLCYSYENNL
CCCEEEEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCH
LALKQALQLLDLLYPDHKLNYNRVISVVEQSSIFTPFQWIDALLVGKANRAKRILKGLQA
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHCCCC
EDVQPVILLRTLQRELFTLLELTKPQQRIVTTEKLPIQQIKTEFDRLKIWQNRRPLFLSA
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHH
IQRLTYQTLYEIIQELANIERLAKQEFSDEVWIKLADLSVKICL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800