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The map label for this gene is thyA [H]

Identifier: 16272842

GI number: 16272842

Start: 959511

End: 960362

Strand: Direct

Name: thyA [H]

Synonym: HI0905

Alternate gene names: 16272842

Gene position: 959511-960362 (Clockwise)

Preceding gene: 16272841

Following gene: 16272843

Centisome position: 52.43

GC content: 40.49

Gene sequence:

>852_bases
ATGAAGCAATATCTTGAGCTTTGTCGCCGCATTGTTAGTGAAGGGGAATGGGTTGCTAATGAACGTACAGGTAAGCATTG
CCTCACGGTCATTAATGCAGATTTAGAATATGATGTGGCGAATAATCAATTTCCGCTGATTACTACCCGTAAAAGTTATT
GGAAAGCGGCGATTGCTGAATTTTTAGGTTATATTCGTGGATATGACAATGCCGCTGATTTCCGCGCACTTGGCACGAAA
ACTTGGGATGCTAATGCGAATGAAAATGCAGCTTGGCTTGCAAATCCGCATCGTAGAGGCGTTGATGATATGGGGCGCGT
ATATGGTGTGCAAGGCAGAGCATGGCGTAAGCCTAATGGAGAAACTATCGATCAGCTACGTAAAATTGTTAATAACTTAA
CGAAAGGTATTGATGATAGAGGAGAGATTTTAACTTTTTTTAACCCTGGGGAATTTGATCTTGGTTGTCTTCGTCCTTGT
ATGCATACGCATACTTTTTCTCTTGTGGGCGATACTTTACATCTTACTAGCTATCAGCGTTCCTGTGATGTTCCGCTTGG
ATTGAATTTCAATCAAATTCAGGTGTTTACCTTCTTAGCACTTATGGCTCAGATCACGGGCAAAAAAGCCGGCAAGGCAT
ATCATAAAATTGTGAATGCGCATATTTATGAAGATCAGCTTGAGTTAATGCGTGACGTACAACTTAAACGCGAGCCTTTC
CCATTACCAAAATTAGAAATTAATCCAGATATAAAAACGCTTGAAGATTTGGAAACTTGGGTCACGATGGATGATTTTAA
AGTCGTTGGCTATCAATCCCACGAACCAATTAAATATCCTTTTTCCGTCTAA

Upstream 100 bases:

>100_bases
ACGAGGGCAAGCCCTTTGCTTGCCGATGATTATTGGTGGTGCTTTCATTATGGCTTGGGCTTATTCACGCAAAAGTGCGG
TCATAAAATAGGAGATTTTT

Downstream 100 bases:

>100_bases
TGGATGCAGCAAAAGTGCGGTCAGAATTTGACGAGAAAATGATGCGCTACGCCCTTGAGCTTGCCGATAAAGCGGAAGCG
TTAGGTGAGATTCCTGTGGG

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MKQYLELCRRIVSEGEWVANERTGKHCLTVINADLEYDVANNQFPLITTRKSYWKAAIAEFLGYIRGYDNAADFRALGTK
TWDANANENAAWLANPHRRGVDDMGRVYGVQGRAWRKPNGETIDQLRKIVNNLTKGIDDRGEILTFFNPGEFDLGCLRPC
MHTHTFSLVGDTLHLTSYQRSCDVPLGLNFNQIQVFTFLALMAQITGKKAGKAYHKIVNAHIYEDQLELMRDVQLKREPF
PLPKLEINPDIKTLEDLETWVTMDDFKVVGYQSHEPIKYPFSV

Sequences:

>Translated_283_residues
MKQYLELCRRIVSEGEWVANERTGKHCLTVINADLEYDVANNQFPLITTRKSYWKAAIAEFLGYIRGYDNAADFRALGTK
TWDANANENAAWLANPHRRGVDDMGRVYGVQGRAWRKPNGETIDQLRKIVNNLTKGIDDRGEILTFFNPGEFDLGCLRPC
MHTHTFSLVGDTLHLTSYQRSCDVPLGLNFNQIQVFTFLALMAQITGKKAGKAYHKIVNAHIYEDQLELMRDVQLKREPF
PLPKLEINPDIKTLEDLETWVTMDDFKVVGYQSHEPIKYPFSV
>Mature_283_residues
MKQYLELCRRIVSEGEWVANERTGKHCLTVINADLEYDVANNQFPLITTRKSYWKAAIAEFLGYIRGYDNAADFRALGTK
TWDANANENAAWLANPHRRGVDDMGRVYGVQGRAWRKPNGETIDQLRKIVNNLTKGIDDRGEILTFFNPGEFDLGCLRPC
MHTHTFSLVGDTLHLTSYQRSCDVPLGLNFNQIQVFTFLALMAQITGKKAGKAYHKIVNAHIYEDQLELMRDVQLKREPF
PLPKLEINPDIKTLEDLETWVTMDDFKVVGYQSHEPIKYPFSV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=289, Percent_Identity=32.8719723183391, Blast_Score=157, Evalue=1e-38,
Organism=Escherichia coli, GI1789191, Length=283, Percent_Identity=33.9222614840989, Blast_Score=157, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI71993377, Length=276, Percent_Identity=28.2608695652174, Blast_Score=130, Evalue=9e-31,
Organism=Saccharomyces cerevisiae, GI6324648, Length=302, Percent_Identity=27.8145695364238, Blast_Score=110, Evalue=2e-25,
Organism=Drosophila melanogaster, GI17137556, Length=291, Percent_Identity=30.2405498281787, Blast_Score=137, Evalue=6e-33,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 32460; Mature: 32460

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYLELCRRIVSEGEWVANERTGKHCLTVINADLEYDVANNQFPLITTRKSYWKAAIAE
CHHHHHHHHHHHCCCCEEECCCCCCEEEEEEECCEEEEECCCCCCEEECCHHHHHHHHHH
FLGYIRGYDNAADFRALGTKTWDANANENAAWLANPHRRGVDDMGRVYGVQGRAWRKPNG
HHHHHHCCCCCHHHHHHCCCCCCCCCCCCCEEECCCCCCCHHHHHHHCCCCCCCCCCCCC
ETIDQLRKIVNNLTKGIDDRGEILTFFNPGEFDLGCLRPCMHTHTFSLVGDTLHLTSYQR
HHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEHHHHH
SCDVPLGLNFNQIQVFTFLALMAQITGKKAGKAYHKIVNAHIYEDQLELMRDVQLKREPF
CCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PLPKLEINPDIKTLEDLETWVTMDDFKVVGYQSHEPIKYPFSV
CCCCEEECCCHHHHHHHHHHEEECCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MKQYLELCRRIVSEGEWVANERTGKHCLTVINADLEYDVANNQFPLITTRKSYWKAAIAE
CHHHHHHHHHHHCCCCEEECCCCCCEEEEEEECCEEEEECCCCCCEEECCHHHHHHHHHH
FLGYIRGYDNAADFRALGTKTWDANANENAAWLANPHRRGVDDMGRVYGVQGRAWRKPNG
HHHHHHCCCCCHHHHHHCCCCCCCCCCCCCEEECCCCCCCHHHHHHHCCCCCCCCCCCCC
ETIDQLRKIVNNLTKGIDDRGEILTFFNPGEFDLGCLRPCMHTHTFSLVGDTLHLTSYQR
HHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEHHHHH
SCDVPLGLNFNQIQVFTFLALMAQITGKKAGKAYHKIVNAHIYEDQLELMRDVQLKREPF
CCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PLPKLEINPDIKTLEDLETWVTMDDFKVVGYQSHEPIKYPFSV
CCCCEEECCCHHHHHHHHHHEEECCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA