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The map label for this gene is emrB

Identifier: 16272835

GI number: 16272835

Start: 952635

End: 954167

Strand: Reverse

Name: emrB

Synonym: HI0897

Alternate gene names: 16272835

Gene position: 954167-952635 (Counterclockwise)

Preceding gene: 16272836

Following gene: 16272824

Centisome position: 52.14

GC content: 39.6

Gene sequence:

>1533_bases
ATGGGCAATTCCGCAAAAAAATTCCCGCCTATTCAAGGTGGTGCGCTGATTTTATTAACGCTGGCTTTATCTCTTGCCAC
CTTTATGCAGGTATTAGATTCTACCATTGCAAATGTGGCAATTCCTACTATTGCGGGCGATCTTGGCGCATCTTTTAGTC
AAGGCACGTGGGTAATTACTTCTTTTGGGGTAGCAAATGCGATTTCTATTCCAATAACGGGTTGGTTAGCAAAACGTTTT
GGCGAAGTGCGGTTATTTTTAGTCTCAACTTTTTTATTTGTTGTCTCTTCTTGGCTCTGTGGGATTGCAGATAGTCTTGA
GGCGTTAATTATTTTTCGCGTCATTCAAGGTGCTGTCGCAGGCCCAGTGATCCCTCTTTCGCAAAGTTTATTATTAAATA
ATTATCCACCAGAAAAACGCGGAATGGCATTGGCATTTTGGTCAATGACTATTGTGGTTGCGCCTATTTTTGGCCCAATT
TTAGGCGGATGGATTAGCGATAATATCCATTGGGGATGGATCTTTTTTATCAATGTTCCGATCGGTTTGTCAGTTGTGCT
AATCAGCTGGAAGATTTTGGGAAGCAGAGAAAGTGAAATTGTTCATCAACCTATTGATAAAGTCGGTTTAGTTTTACTTG
TCTTAGGCGTGGGATGTTTGCAATTAATGCTTGATCAGGGTCGTGAGCAAGATTGGTTTAATTCTAATGAAATCATTATA
TTAGCCGTTGTTGCTGTCGTTTGTCTGATTGCCTTGGTTATTTGGGAATTGACAGATGACAATCCTGTAGTGGATATTTC
GCTTTTCCATTCTCGTAATTTTAGCGTAGGGTGTTTATGTACTAGTTTAGCCTTTTTGATTTATCTAGGTTCTGTGGTAT
TAATTCCGCTCTTGCTACAACAAGTCTTCCATTACACTGCGACGTGGGCAGGGCTTGCAGCTTCCCCAGTTGGATTATTC
CCAATTTTGCTTTCACCGATTATTGGGCGTTTCGGTTATAAGATCGATATGCGAATTTTAGTGACGATAAGCTTTATTGT
ATATGCAATAACATTTTATTGGCGAGCGGTAACTTTCGAGCCATCAATGACATTTGTTGATGTAGCTTTACCACAATTAG
TGCAAGGTTTAGCGGTGTCCTGTTTCTTTATGCCACTTACTACAATAACGCTGTCTGGCTTGCCTGCGCATAAAATGGCT
TCAGCCTCTAGCTTATTTAACTTTTTGAGAACTTTAGCGGGTTCTGTAGGAACATCTTTGACCACATTTATGTGGTATAA
CCGTGAAGCTGTTCACCACACTCAATTAACAGAACACATTAATCCTTATAACCCAATTTCCCAAAGTTTTTACCATCAAA
TGAATCAATTTGGTTTAAGTGATACGCAAACTTCAGCTTACCTCGCTCAACAAATAACATCGCAAGGATTTATTATTGGC
GCAAATGAAATATTTTGGTTATCCGCTATGGGATTCTTAGGATTGCTTATTGTGATTTGGTTTGCAAAACCACCTTTTGG
TACTCAGCATTAA

Upstream 100 bases:

>100_bases
GAAAACGTTATTTTCTACTGATACACTTAAATATGATGAAAGTGCGGTAGAAAATTTGATTGAATCTATCATTCAGCAAA
ACAGCCATTAAGGCGGCGTT

Downstream 100 bases:

>100_bases
AATAAATAAATATATATTACATACCAATCACAACACAATCGGTAACAGTTTTGGCTCTACTTTGCGCCTGTACTGCAAGC
TCTCGAGTATCAAAACTTGC

Product: multidrug resistance protein B

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MGNSAKKFPPIQGGALILLTLALSLATFMQVLDSTIANVAIPTIAGDLGASFSQGTWVITSFGVANAISIPITGWLAKRF
GEVRLFLVSTFLFVVSSWLCGIADSLEALIIFRVIQGAVAGPVIPLSQSLLLNNYPPEKRGMALAFWSMTIVVAPIFGPI
LGGWISDNIHWGWIFFINVPIGLSVVLISWKILGSRESEIVHQPIDKVGLVLLVLGVGCLQLMLDQGREQDWFNSNEIII
LAVVAVVCLIALVIWELTDDNPVVDISLFHSRNFSVGCLCTSLAFLIYLGSVVLIPLLLQQVFHYTATWAGLAASPVGLF
PILLSPIIGRFGYKIDMRILVTISFIVYAITFYWRAVTFEPSMTFVDVALPQLVQGLAVSCFFMPLTTITLSGLPAHKMA
SASSLFNFLRTLAGSVGTSLTTFMWYNREAVHHTQLTEHINPYNPISQSFYHQMNQFGLSDTQTSAYLAQQITSQGFIIG
ANEIFWLSAMGFLGLLIVIWFAKPPFGTQH

Sequences:

>Translated_510_residues
MGNSAKKFPPIQGGALILLTLALSLATFMQVLDSTIANVAIPTIAGDLGASFSQGTWVITSFGVANAISIPITGWLAKRF
GEVRLFLVSTFLFVVSSWLCGIADSLEALIIFRVIQGAVAGPVIPLSQSLLLNNYPPEKRGMALAFWSMTIVVAPIFGPI
LGGWISDNIHWGWIFFINVPIGLSVVLISWKILGSRESEIVHQPIDKVGLVLLVLGVGCLQLMLDQGREQDWFNSNEIII
LAVVAVVCLIALVIWELTDDNPVVDISLFHSRNFSVGCLCTSLAFLIYLGSVVLIPLLLQQVFHYTATWAGLAASPVGLF
PILLSPIIGRFGYKIDMRILVTISFIVYAITFYWRAVTFEPSMTFVDVALPQLVQGLAVSCFFMPLTTITLSGLPAHKMA
SASSLFNFLRTLAGSVGTSLTTFMWYNREAVHHTQLTEHINPYNPISQSFYHQMNQFGLSDTQTSAYLAQQITSQGFIIG
ANEIFWLSAMGFLGLLIVIWFAKPPFGTQH
>Mature_509_residues
GNSAKKFPPIQGGALILLTLALSLATFMQVLDSTIANVAIPTIAGDLGASFSQGTWVITSFGVANAISIPITGWLAKRFG
EVRLFLVSTFLFVVSSWLCGIADSLEALIIFRVIQGAVAGPVIPLSQSLLLNNYPPEKRGMALAFWSMTIVVAPIFGPIL
GGWISDNIHWGWIFFINVPIGLSVVLISWKILGSRESEIVHQPIDKVGLVLLVLGVGCLQLMLDQGREQDWFNSNEIIIL
AVVAVVCLIALVIWELTDDNPVVDISLFHSRNFSVGCLCTSLAFLIYLGSVVLIPLLLQQVFHYTATWAGLAASPVGLFP
ILLSPIIGRFGYKIDMRILVTISFIVYAITFYWRAVTFEPSMTFVDVALPQLVQGLAVSCFFMPLTTITLSGLPAHKMAS
ASSLFNFLRTLAGSVGTSLTTFMWYNREAVHHTQLTEHINPYNPISQSFYHQMNQFGLSDTQTSAYLAQQITSQGFIIGA
NEIFWLSAMGFLGLLIVIWFAKPPFGTQH

Specific function: Translocase that confers resistance to substances of high hydrophobicity

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family

Homologues:

Organism=Escherichia coli, GI1789042, Length=501, Percent_Identity=67.6646706586826, Blast_Score=706, Evalue=0.0,
Organism=Escherichia coli, GI1788710, Length=500, Percent_Identity=57.6, Blast_Score=585, Evalue=1e-168,
Organism=Escherichia coli, GI1790195, Length=426, Percent_Identity=30.9859154929577, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI87081983, Length=419, Percent_Identity=26.2529832935561, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1788392, Length=399, Percent_Identity=28.5714285714286, Blast_Score=122, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6322958, Length=199, Percent_Identity=27.1356783919598, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=30, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6325455, Length=126, Percent_Identity=26.984126984127, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): EMRB_HAEIN (P44927)

Other databases:

- EMBL:   L42023
- PIR:   A64101
- RefSeq:   NP_439058.1
- ProteinModelPortal:   P44927
- GeneID:   949901
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0897
- NMPDR:   fig|71421.1.peg.864
- TIGR:   HI_0897
- HOGENOM:   HBG759108
- OMA:   IWFAKPP
- ProtClustDB:   CLSK880460
- BioCyc:   HINF71421:HI_0897-MONOMER
- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- TIGRFAMs:   TIGR00711

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 55827; Mature: 55696

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xb49b1e4)-; HASH(0xcee7734)-; HASH(0xc4894a8)-; HASH(0xb74a730)-; HASH(0xc46c048)-; HASH(0xb3a2184)-; HASH(0xbd93e18)-; HASH(0xb74a4fc)-; HASH(0xc441fa8)-; HASH(0xda31a24)-; HASH(0xda8922c)-; HASH(0xc441df8)-; HASH(0xcf12b08)-; HASH(0xd40cc24)-;

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNSAKKFPPIQGGALILLTLALSLATFMQVLDSTIANVAIPTIAGDLGASFSQGTWVIT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
SFGVANAISIPITGWLAKRFGEVRLFLVSTFLFVVSSWLCGIADSLEALIIFRVIQGAVA
ECCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GPVIPLSQSLLLNNYPPEKRGMALAFWSMTIVVAPIFGPILGGWISDNIHWGWIFFINVP
CCCCCCCHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECC
IGLSVVLISWKILGSRESEIVHQPIDKVGLVLLVLGVGCLQLMLDQGREQDWFNSNEIII
CCCEEHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
LAVVAVVCLIALVIWELTDDNPVVDISLFHSRNFSVGCLCTSLAFLIYLGSVVLIPLLLQ
HHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QVFHYTATWAGLAASPVGLFPILLSPIIGRFGYKIDMRILVTISFIVYAITFYWRAVTFE
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHHHEEEC
PSMTFVDVALPQLVQGLAVSCFFMPLTTITLSGLPAHKMASASSLFNFLRTLAGSVGTSL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHH
TTFMWYNREAVHHTQLTEHINPYNPISQSFYHQMNQFGLSDTQTSAYLAQQITSQGFIIG
HHHHEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEE
ANEIFWLSAMGFLGLLIVIWFAKPPFGTQH
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCH
>Mature Secondary Structure 
GNSAKKFPPIQGGALILLTLALSLATFMQVLDSTIANVAIPTIAGDLGASFSQGTWVIT
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
SFGVANAISIPITGWLAKRFGEVRLFLVSTFLFVVSSWLCGIADSLEALIIFRVIQGAVA
ECCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GPVIPLSQSLLLNNYPPEKRGMALAFWSMTIVVAPIFGPILGGWISDNIHWGWIFFINVP
CCCCCCCHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECC
IGLSVVLISWKILGSRESEIVHQPIDKVGLVLLVLGVGCLQLMLDQGREQDWFNSNEIII
CCCEEHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
LAVVAVVCLIALVIWELTDDNPVVDISLFHSRNFSVGCLCTSLAFLIYLGSVVLIPLLLQ
HHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QVFHYTATWAGLAASPVGLFPILLSPIIGRFGYKIDMRILVTISFIVYAITFYWRAVTFE
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHHHEEEC
PSMTFVDVALPQLVQGLAVSCFFMPLTTITLSGLPAHKMASASSLFNFLRTLAGSVGTSL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHH
TTFMWYNREAVHHTQLTEHINPYNPISQSFYHQMNQFGLSDTQTSAYLAQQITSQGFIIG
HHHHEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEE
ANEIFWLSAMGFLGLLIVIWFAKPPFGTQH
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800