Click here to switch to the map view.

The map label for this gene is emrA

Identifier: 16272836

GI number: 16272836

Start: 954177

End: 955349

Strand: Reverse

Name: emrA

Synonym: HI0898

Alternate gene names: 16272836

Gene position: 955349-954177 (Counterclockwise)

Preceding gene: 16272837

Following gene: 16272835

Centisome position: 52.2

GC content: 38.62

Gene sequence:

>1173_bases
ATGACGCAAATTGCAACTGAAAACCCATCAACTAAATCTGTATCAAATAAAACTGACCGTAAAAAAGGCTTAAGCATTTT
TATTCTTCTCCTGCTTATTATTGGTATTGCTTGTGCTTTGTATTGGTTTTTCTTCTTGAAGGATTTTGAAGAAACAGAAG
ATGCTTATGTGGGTGGAAATCAAGTAATGGTATCATCACAAGTTGCAGGTAATGTAGCGAAGATTAATGCAGACAATATG
GATAAAGTCCATGCAGGTGATATTCTAGTTGAATTGGATGATACCAATGCGAAACTTAGTTTCGAACAGGCCAAAAGCAA
TCTCGCCAATGCTGTACGTCAGGTAGAACAACTGGGTTTCACTGTGCAGCAATTGCAATCAGCTGTGCACGCCAATGAAA
TTTCTTTAGCTCAAGCTCAAGGCAATTTGGCGCGCCGAGTTCAACTTGAAAAAATGGGTGCGATTGATAAAGAATCTTTC
CAACACGCAAAAGAAGCTGTCGAGCTTGCCAAAGCAAATTTAAATGCCTCTAAAAATCAATTGGCTGCTAATCAGGCTTT
ATTGCGTAACGTTCCATTACGTGAACAACCGCAAATACAGAATGCGATAAACTCGCTTAAACAGGCTTGGTTAAATTTGC
AACGTACAAAAATTAGAAGTCCAATTGATGGCTATGTGGCGCGTCGTAATGTTCAAGTTGGGCAAGCCGTTTCAGTGGGT
GGCGCGTTAATGGCAGTGGTATCTAATGAACAAATGTGGCTAGAAGCTAACTTCAAAGAAACCCAATTAACGAATATGCG
CATTGGTCAGCCAGTAAAAATCCATTTTGATTTATACGGTAAAAATAAAGAATTTGATGGCGTGATAAATGGTATTGAAA
TGGGGACTGGCAATGCATTTTCTCTTTTGCCTTCACAAAATGCTACGGGTAACTGGATTAAAGTGGTGCAACGCGTACCT
GTGCGAATTAAATTAGATCCACAACAATTTACAGAAACGCCATTGAGAATTGGTCTTTCTGCAACGGCAAAAGTAAGAAT
TTCAGATTCATCAGGCGCAATGCTACGAGAAAAAACAGAACCGAAAACGTTATTTTCTACTGATACACTTAAATATGATG
AAAGTGCGGTAGAAAATTTGATTGAATCTATCATTCAGCAAAACAGCCATTAA

Upstream 100 bases:

>100_bases
TTAAAGTTTCATAATGACCCTTCATTTTTATTTTTGATAGTTTTAAAGGTTATCTCAAATATCTCATTTTTAAACATTAT
CCCAGCTTAGGGAAGGCATT

Downstream 100 bases:

>100_bases
GGCGGCGTTATGGGCAATTCCGCAAAAAAATTCCCGCCTATTCAAGGTGGTGCGCTGATTTTATTAACGCTGGCTTTATC
TCTTGCCACCTTTATGCAGG

Product: multidrug resistance protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MTQIATENPSTKSVSNKTDRKKGLSIFILLLLIIGIACALYWFFFLKDFEETEDAYVGGNQVMVSSQVAGNVAKINADNM
DKVHAGDILVELDDTNAKLSFEQAKSNLANAVRQVEQLGFTVQQLQSAVHANEISLAQAQGNLARRVQLEKMGAIDKESF
QHAKEAVELAKANLNASKNQLAANQALLRNVPLREQPQIQNAINSLKQAWLNLQRTKIRSPIDGYVARRNVQVGQAVSVG
GALMAVVSNEQMWLEANFKETQLTNMRIGQPVKIHFDLYGKNKEFDGVINGIEMGTGNAFSLLPSQNATGNWIKVVQRVP
VRIKLDPQQFTETPLRIGLSATAKVRISDSSGAMLREKTEPKTLFSTDTLKYDESAVENLIESIIQQNSH

Sequences:

>Translated_390_residues
MTQIATENPSTKSVSNKTDRKKGLSIFILLLLIIGIACALYWFFFLKDFEETEDAYVGGNQVMVSSQVAGNVAKINADNM
DKVHAGDILVELDDTNAKLSFEQAKSNLANAVRQVEQLGFTVQQLQSAVHANEISLAQAQGNLARRVQLEKMGAIDKESF
QHAKEAVELAKANLNASKNQLAANQALLRNVPLREQPQIQNAINSLKQAWLNLQRTKIRSPIDGYVARRNVQVGQAVSVG
GALMAVVSNEQMWLEANFKETQLTNMRIGQPVKIHFDLYGKNKEFDGVINGIEMGTGNAFSLLPSQNATGNWIKVVQRVP
VRIKLDPQQFTETPLRIGLSATAKVRISDSSGAMLREKTEPKTLFSTDTLKYDESAVENLIESIIQQNSH
>Mature_389_residues
TQIATENPSTKSVSNKTDRKKGLSIFILLLLIIGIACALYWFFFLKDFEETEDAYVGGNQVMVSSQVAGNVAKINADNMD
KVHAGDILVELDDTNAKLSFEQAKSNLANAVRQVEQLGFTVQQLQSAVHANEISLAQAQGNLARRVQLEKMGAIDKESFQ
HAKEAVELAKANLNASKNQLAANQALLRNVPLREQPQIQNAINSLKQAWLNLQRTKIRSPIDGYVARRNVQVGQAVSVGG
ALMAVVSNEQMWLEANFKETQLTNMRIGQPVKIHFDLYGKNKEFDGVINGIEMGTGNAFSLLPSQNATGNWIKVVQRVPV
RIKLDPQQFTETPLRIGLSATAKVRISDSSGAMLREKTEPKTLFSTDTLKYDESAVENLIESIIQQNSH

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family

Homologues:

Organism=Escherichia coli, GI1789041, Length=384, Percent_Identity=46.875, Blast_Score=353, Evalue=8e-99,
Organism=Escherichia coli, GI1788711, Length=379, Percent_Identity=47.2295514511873, Blast_Score=340, Evalue=1e-94,
Organism=Escherichia coli, GI1790519, Length=309, Percent_Identity=28.1553398058252, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI87081951, Length=311, Percent_Identity=26.0450160771704, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789637, Length=326, Percent_Identity=26.0736196319018, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): EMRA_HAEIN (P44928)

Other databases:

- EMBL:   L42023
- PIR:   B64101
- RefSeq:   NP_439059.1
- ProteinModelPortal:   P44928
- GeneID:   949904
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0898
- NMPDR:   fig|71421.1.peg.865
- TIGR:   HI_0898
- HOGENOM:   HBG624965
- OMA:   VRIKLDP
- ProtClustDB:   CLSK808463
- BioCyc:   HINF71421:HI_0898-MONOMER
- InterPro:   IPR005694
- InterPro:   IPR006143
- TIGRFAMs:   TIGR00998

Pfam domain/function: PF00529 HlyD

EC number: NA

Molecular weight: Translated: 43063; Mature: 42931

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: PS00543 HLYD_FAMILY

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xc9ff20c)-;

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQIATENPSTKSVSNKTDRKKGLSIFILLLLIIGIACALYWFFFLKDFEETEDAYVGGN
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCC
QVMVSSQVAGNVAKINADNMDKVHAGDILVELDDTNAKLSFEQAKSNLANAVRQVEQLGF
EEEEEHHHCCCEEEECCCCCCCEECCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHCH
TVQQLQSAVHANEISLAQAQGNLARRVQLEKMGAIDKESFQHAKEAVELAKANLNASKNQ
HHHHHHHHHHHCHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHH
LAANQALLRNVPLREQPQIQNAINSLKQAWLNLQRTKIRSPIDGYVARRNVQVGQAVSVG
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHC
GALMAVVSNEQMWLEANFKETQLTNMRIGQPVKIHFDLYGKNKEFDGVINGIEMGTGNAF
CEEEEEECCCEEEEECCCCHHEECCCCCCCCEEEEEEEECCCCCCCHHHCCEEECCCCEE
SLLPSQNATGNWIKVVQRVPVRIKLDPQQFTETPLRIGLSATAKVRISDSSGAMLREKTE
EECCCCCCCCHHHHHHHHCCEEEEECHHHHHCCCHHCCCCCEEEEEEECCCCCEEECCCC
PKTLFSTDTLKYDESAVENLIESIIQQNSH
CCEEEECCCCCCCHHHHHHHHHHHHHCCCH
>Mature Secondary Structure 
TQIATENPSTKSVSNKTDRKKGLSIFILLLLIIGIACALYWFFFLKDFEETEDAYVGGN
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCC
QVMVSSQVAGNVAKINADNMDKVHAGDILVELDDTNAKLSFEQAKSNLANAVRQVEQLGF
EEEEEHHHCCCEEEECCCCCCCEECCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHCH
TVQQLQSAVHANEISLAQAQGNLARRVQLEKMGAIDKESFQHAKEAVELAKANLNASKNQ
HHHHHHHHHHHCHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHH
LAANQALLRNVPLREQPQIQNAINSLKQAWLNLQRTKIRSPIDGYVARRNVQVGQAVSVG
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHC
GALMAVVSNEQMWLEANFKETQLTNMRIGQPVKIHFDLYGKNKEFDGVINGIEMGTGNAF
CEEEEEECCCEEEEECCCCHHEECCCCCCCCEEEEEEEECCCCCCCHHHCCEEECCCCEE
SLLPSQNATGNWIKVVQRVPVRIKLDPQQFTETPLRIGLSATAKVRISDSSGAMLREKTE
EECCCCCCCCHHHHHHHHCCEEEEECHHHHHCCCHHCCCCCEEEEEEECCCCCEEECCCC
PKTLFSTDTLKYDESAVENLIESIIQQNSH
CCEEEECCCCCCCHHHHHHHHHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800