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The map label for this gene is purH

Identifier: 16272827

GI number: 16272827

Start: 939856

End: 941454

Strand: Direct

Name: purH

Synonym: HI0887

Alternate gene names: 16272827

Gene position: 939856-941454 (Clockwise)

Preceding gene: 16272826

Following gene: 16272828

Centisome position: 51.35

GC content: 42.46

Gene sequence:

>1599_bases
ATGGCAGATCGTCCAATTCGTCAGGCTTTACTGAGTGTGTCTGATAAAACGGGTATTGTAGAGTTTGCTCAAGGTTTAGT
TAAACGTGGTGTAAAACTACTTTCAACAGGTGGAACGGCAAAACTTTTAGCACAAAATGCTTTACCTGTAATAGAAGTGT
CTGATTACACAGGTTTCCCAGAAATGATGGACGGTCGAGTGAAAACCTTACATCCCAAAGTACATGGCGGTATTCTTGGT
CGTCGTGGTACAGATGATGCCATCATGCAGCAACATGGCATTGAAGGCATTGATATGGTCGTTGTGAATTTATATCCCTT
TGCTGCCACTGTGGCAAAACCTGATTGCACTTTGGCTGATGCGGTAGAAAATATCGATATTGGGGGGCCTACAATGGTGC
GTTCTGCAGCGAAAAACCACAAAGATGTAGCGATCGTGGTTAATAATCATGATTTCAACGCAATTCTAGCCGAAATGGAT
CAACATCAAAACAGCCTAACTTTTGAAACTCGTTTTGACCTTGCGATTAAAGCATTTGAACATACCGCTCAATATGATTC
TATGATTGCCAACTATTTCGGTCAGCTAGTAAAACCTTATCATATTGCAGAGGAAGAAGAGGCGAATGCGAAGTGCGGTC
AATTCCCACGGACTTTAAACCTTAACTTCGTGCGTAAACAAGCTATGCGTTACGGCGAAAACTCCCATCAAAATGCGGCA
TTTTATGTTGATTTAAATGTGAAAGAAGCGAGCGTGGCTACAGCTAATCAACTGCAAGGTAAAGCCTTGTCTTACAATAA
TATTGCCGACACTGATGCAGCACTTGAATGCGTGAAAGAATTTGACGATCCAGCTTGCGTAATCGTTAAACATGCCAATC
CATGTGGTGTGGCGTTAGGTAAGGATATTTTAGACGCTTATAATCGCGCTTACCAAACCGATCCAACGTCTGCTTTTGGC
GGCATTATTGCTTTTAACCGTGAATTAGACGAAAAAACGGCGAATGAAATTGTGGAACGCCAATTCGTTGAAGTGATTAT
CGCACCGAAAGTTTCTGCTGAAGCGCAAGAAGTAATGAAGCGTAAGAAAAATGTGCGTTTGCTTGAATGTGGTGAATGGA
CTTCTCGTTCCGAACGTTTGGATTTCAAACGTGTAAACGGCGGTTTATTAGTACAAGATGCGGATTTAGGTATGGTTGGC
GTGGATGATTTAAAAGTCGTGAGTAAACGTCAGCCAACTGAACAAGAATTAAAAGACTTATTATTCTGCTGGAAAGTGGC
AAAATTTGTGAAATCGAATGCCATTGTTTACGCCAAAGACAATCAAACTATCGGCATTGGTGCAGGTCAAATGAGCCGCG
TATATTCTGCGAAGATTGCGGGTATTAAAGCGCAAGATGAAGGTTTAGAAGTGGCTGGTTGTGTGATGGCATCTGATGCG
TTCTTCCCATTCCGTGACGGCATTGATGCGGCGGCGAAAGTGGGTATTCAATGTGTGATCCATCCAGGTGGATCAATGCG
CGATCAAGAAGTCATTGATGCGGCGGATGAACATAATATGGTAATGGTATTGACTGGAATGCGACATTTTAGACATTAA

Upstream 100 bases:

>100_bases
CACTTTAAGAAATTATATTTAATCTTTTCTATATTTAAGCAAACGTTTGCGTTATAATTCTCATCGCTTATTTTTAACTA
TTTAATGAAAGGAAAATACA

Downstream 100 bases:

>100_bases
TTTATCTAACTATCCCCCTCTTTACAAAAGAGGGGGAATTGCAGGTAAAAAATAGCCGTACTTTTTTTAAATTAGGCATA
CGCAAGGACTACAACATGAA

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase

Number of amino acids: Translated: 532; Mature: 531

Protein sequence:

>532_residues
MADRPIRQALLSVSDKTGIVEFAQGLVKRGVKLLSTGGTAKLLAQNALPVIEVSDYTGFPEMMDGRVKTLHPKVHGGILG
RRGTDDAIMQQHGIEGIDMVVVNLYPFAATVAKPDCTLADAVENIDIGGPTMVRSAAKNHKDVAIVVNNHDFNAILAEMD
QHQNSLTFETRFDLAIKAFEHTAQYDSMIANYFGQLVKPYHIAEEEEANAKCGQFPRTLNLNFVRKQAMRYGENSHQNAA
FYVDLNVKEASVATANQLQGKALSYNNIADTDAALECVKEFDDPACVIVKHANPCGVALGKDILDAYNRAYQTDPTSAFG
GIIAFNRELDEKTANEIVERQFVEVIIAPKVSAEAQEVMKRKKNVRLLECGEWTSRSERLDFKRVNGGLLVQDADLGMVG
VDDLKVVSKRQPTEQELKDLLFCWKVAKFVKSNAIVYAKDNQTIGIGAGQMSRVYSAKIAGIKAQDEGLEVAGCVMASDA
FFPFRDGIDAAAKVGIQCVIHPGGSMRDQEVIDAADEHNMVMVLTGMRHFRH

Sequences:

>Translated_532_residues
MADRPIRQALLSVSDKTGIVEFAQGLVKRGVKLLSTGGTAKLLAQNALPVIEVSDYTGFPEMMDGRVKTLHPKVHGGILG
RRGTDDAIMQQHGIEGIDMVVVNLYPFAATVAKPDCTLADAVENIDIGGPTMVRSAAKNHKDVAIVVNNHDFNAILAEMD
QHQNSLTFETRFDLAIKAFEHTAQYDSMIANYFGQLVKPYHIAEEEEANAKCGQFPRTLNLNFVRKQAMRYGENSHQNAA
FYVDLNVKEASVATANQLQGKALSYNNIADTDAALECVKEFDDPACVIVKHANPCGVALGKDILDAYNRAYQTDPTSAFG
GIIAFNRELDEKTANEIVERQFVEVIIAPKVSAEAQEVMKRKKNVRLLECGEWTSRSERLDFKRVNGGLLVQDADLGMVG
VDDLKVVSKRQPTEQELKDLLFCWKVAKFVKSNAIVYAKDNQTIGIGAGQMSRVYSAKIAGIKAQDEGLEVAGCVMASDA
FFPFRDGIDAAAKVGIQCVIHPGGSMRDQEVIDAADEHNMVMVLTGMRHFRH
>Mature_531_residues
ADRPIRQALLSVSDKTGIVEFAQGLVKRGVKLLSTGGTAKLLAQNALPVIEVSDYTGFPEMMDGRVKTLHPKVHGGILGR
RGTDDAIMQQHGIEGIDMVVVNLYPFAATVAKPDCTLADAVENIDIGGPTMVRSAAKNHKDVAIVVNNHDFNAILAEMDQ
HQNSLTFETRFDLAIKAFEHTAQYDSMIANYFGQLVKPYHIAEEEEANAKCGQFPRTLNLNFVRKQAMRYGENSHQNAAF
YVDLNVKEASVATANQLQGKALSYNNIADTDAALECVKEFDDPACVIVKHANPCGVALGKDILDAYNRAYQTDPTSAFGG
IIAFNRELDEKTANEIVERQFVEVIIAPKVSAEAQEVMKRKKNVRLLECGEWTSRSERLDFKRVNGGLLVQDADLGMVGV
DDLKVVSKRQPTEQELKDLLFCWKVAKFVKSNAIVYAKDNQTIGIGAGQMSRVYSAKIAGIKAQDEGLEVAGCVMASDAF
FPFRDGIDAAAKVGIQCVIHPGGSMRDQEVIDAADEHNMVMVLTGMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family

Homologues:

Organism=Homo sapiens, GI20127454, Length=491, Percent_Identity=38.0855397148676, Blast_Score=266, Evalue=3e-71,
Organism=Escherichia coli, GI1790439, Length=529, Percent_Identity=77.1266540642722, Blast_Score=845, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=623, Percent_Identity=32.4237560192616, Blast_Score=254, Evalue=7e-68,
Organism=Caenorhabditis elegans, GI71985574, Length=351, Percent_Identity=27.0655270655271, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI71985556, Length=60, Percent_Identity=51.6666666666667, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323768, Length=492, Percent_Identity=34.349593495935, Blast_Score=254, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6323056, Length=496, Percent_Identity=34.8790322580645, Blast_Score=249, Evalue=6e-67,
Organism=Drosophila melanogaster, GI24649832, Length=494, Percent_Identity=38.2591093117409, Blast_Score=280, Evalue=2e-75,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PUR9_HAEIN (P43852)

Other databases:

- EMBL:   L42023
- PIR:   B64100
- RefSeq:   NP_439048.1
- ProteinModelPortal:   P43852
- SMR:   P43852
- GeneID:   949891
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0887
- NMPDR:   fig|71421.1.peg.854
- TIGR:   HI_0887
- HOGENOM:   HBG498048
- OMA:   ASDGFFP
- ProtClustDB:   PRK00881
- BioCyc:   HINF71421:HI_0887-MONOMER
- BRENDA:   2.1.2.3
- BRENDA:   3.5.4.10
- HAMAP:   MF_00139
- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607
- Gene3D:   G3DSA:3.40.50.1380
- PANTHER:   PTHR11692
- PIRSF:   PIRSF000414
- SMART:   SM00798
- SMART:   SM00851
- TIGRFAMs:   TIGR00355

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS; SSF53927 Cytidine_deaminase-like; SSF52335 MGS-like_dom

EC number: =2.1.2.3; =3.5.4.10

Molecular weight: Translated: 58350; Mature: 58219

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADRPIRQALLSVSDKTGIVEFAQGLVKRGVKLLSTGGTAKLLAQNALPVIEVSDYTGFP
CCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCEEECCCCCHHHHHHCCCCEEEECCCCCCH
EMMDGRVKTLHPKVHGGILGRRGTDDAIMQQHGIEGIDMVVVNLYPFAATVAKPDCTLAD
HHHCCCEEEECCHHHCCCCCCCCCCHHHHHHCCCCCEEEEEEEEEHHHHHCCCCCCHHHH
AVENIDIGGPTMVRSAAKNHKDVAIVVNNHDFNAILAEMDQHQNSLTFETRFDLAIKAFE
HHHHCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCCCEEEEEEHHHHHHHHH
HTAQYDSMIANYFGQLVKPYHIAEEEEANAKCGQFPRTLNLNFVRKQAMRYGENSHQNAA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEE
FYVDLNVKEASVATANQLQGKALSYNNIADTDAALECVKEFDDPACVIVKHANPCGVALG
EEEEECCCHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHH
KDILDAYNRAYQTDPTSAFGGIIAFNRELDEKTANEIVERQFVEVIIAPKVSAEAQEVMK
HHHHHHHHHHCCCCCCHHHCCEEEECCHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHH
RKKNVRLLECGEWTSRSERLDFKRVNGGLLVQDADLGMVGVDDLKVVSKRQPTEQELKDL
HHCCCEEEECCCCCCCCCCCCCEECCCCEEEEECCCCEECCCHHHHHHCCCCCHHHHHHH
LFCWKVAKFVKSNAIVYAKDNQTIGIGAGQMSRVYSAKIAGIKAQDEGLEVAGCVMASDA
HHHHHHHHHHHCCCEEEEECCCEEEECCCHHHHHHHHHHCCCEECCCCCEEEEEEEECCC
FFPFRDGIDAAAKVGIQCVIHPGGSMRDQEVIDAADEHNMVMVLTGMRHFRH
CCCCCCCCCHHHCCCEEEEECCCCCCCCHHHHCCCCCCCEEEEEECHHHHCC
>Mature Secondary Structure 
ADRPIRQALLSVSDKTGIVEFAQGLVKRGVKLLSTGGTAKLLAQNALPVIEVSDYTGFP
CCCHHHHHHHHCCCCCCHHHHHHHHHHCCCEEECCCCCHHHHHHCCCCEEEECCCCCCH
EMMDGRVKTLHPKVHGGILGRRGTDDAIMQQHGIEGIDMVVVNLYPFAATVAKPDCTLAD
HHHCCCEEEECCHHHCCCCCCCCCCHHHHHHCCCCCEEEEEEEEEHHHHHCCCCCCHHHH
AVENIDIGGPTMVRSAAKNHKDVAIVVNNHDFNAILAEMDQHQNSLTFETRFDLAIKAFE
HHHHCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCCCEEEEEEHHHHHHHHH
HTAQYDSMIANYFGQLVKPYHIAEEEEANAKCGQFPRTLNLNFVRKQAMRYGENSHQNAA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEE
FYVDLNVKEASVATANQLQGKALSYNNIADTDAALECVKEFDDPACVIVKHANPCGVALG
EEEEECCCHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHH
KDILDAYNRAYQTDPTSAFGGIIAFNRELDEKTANEIVERQFVEVIIAPKVSAEAQEVMK
HHHHHHHHHHCCCCCCHHHCCEEEECCHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHH
RKKNVRLLECGEWTSRSERLDFKRVNGGLLVQDADLGMVGVDDLKVVSKRQPTEQELKDL
HHCCCEEEECCCCCCCCCCCCCEECCCCEEEEECCCCEECCCHHHHHHCCCCCHHHHHHH
LFCWKVAKFVKSNAIVYAKDNQTIGIGAGQMSRVYSAKIAGIKAQDEGLEVAGCVMASDA
HHHHHHHHHHHCCCEEEEECCCEEEECCCHHHHHHHHHHCCCEECCCCCEEEEEEEECCC
FFPFRDGIDAAAKVGIQCVIHPGGSMRDQEVIDAADEHNMVMVLTGMRHFRH
CCCCCCCCCHHHCCCEEEEECCCCCCCCHHHHCCCCCCCEEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800