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The map label for this gene is ispB

Identifier: 16272821

GI number: 16272821

Start: 932907

End: 933896

Strand: Direct

Name: ispB

Synonym: HI0881

Alternate gene names: 16272821

Gene position: 932907-933896 (Clockwise)

Preceding gene: 16272816

Following gene: 16272822

Centisome position: 50.97

GC content: 39.8

Gene sequence:

>990_bases
ATGAAGAAACAAGATCTTATGAGCATAGACGAGATCCAGAAACTTGCAGATCCAGATATGCAAAAAGTTAATCAAAATAT
TCTTGCTCAGCTTAATTCAGATGTACCGCTAATAGGACAGTTAGGTTTTTATATTGTTCAAGGTGGTGGTAAGCGGATCC
GTCCGTTGATCGCAGTTCTCGCAGCACGATCTTTAGGGTTTGAAGGATCTAATTCAATTACTTGTGCTACTTTTGTTGAA
TTTATTCATACCGCTTCTTTGTTACACGATGATGTCGTAGATGAATCTGATATGCGTCGTGGGCGTGCTACGGCGAATGC
TGAATTTGGTAATGCAGCGAGTGTGTTGGTAGGCGATTTTATTTATACGCGTGCATTTCAATTAGTTGCACAGTTAGAAT
CATTGAAAATTTTAAGTATTATGGCTGATGCGACTAATGTTTTAGCGGAAGGCGAAGTTCAGCAGTTAATGAATGTTAAT
GATCCTGAAACGAGCGAAGCTAATTATATGCGTGTAATTTATAGCAAAACTGCCCGTTTGTTTGAAGTGGCTGGTCAGGC
TGCGGCAATTGTCGCCGGTGGAACAGAAGCTCAAGAAAAGGCTTTACAAGATTATGGGCGTTATCTTGGCACTGCATTTC
AGCTCGTGGATGATGTATTGGATTATAGTGCAAATACGCAAGCTCTAGGTAAAAATGTAGGCGATGATTTAGCTGAAGGA
AAACCAACCCTTCCTCTACTACACGCTATGCGTCATGGAAATGCTCAACAGGCAGCTTTAATTCGCGAAGCGATTGAGCA
AGGTGGTAAACGTGAAGCAATTGATGAAGTATTAGCTATTATGACTGAGCATAAGTCTTTAGATTATGCAATGAATCGTG
CAAAAGAAGAGGCTCAAAAAGCAGTGGATGCAATCGAAATTTTGCCAGAAAGTGAATATAAACAAGCGTTGATTTCATTG
GCATATTTGTCTGTAGATAGAAATTATTAG

Upstream 100 bases:

>100_bases
ATAATAAAATAAAAAAATTCCCTTCATTTGCGGATTGATTTCAGAAAGTCTGAAAATTCGGGTAAAATGCACCGCACTTT
TAACTGATAAATGATGAAAA

Downstream 100 bases:

>100_bases
TCCATAATTTAGCTTATATACTTGATACCCATTAATTTTAATTAAACTTTATGAATACAGAACTTCAACCTAAACTAGAA
AAAAGTGCGGTCAATTTTCA

Product: octaprenyl-diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKKQDLMSIDEIQKLADPDMQKVNQNILAQLNSDVPLIGQLGFYIVQGGGKRIRPLIAVLAARSLGFEGSNSITCATFVE
FIHTASLLHDDVVDESDMRRGRATANAEFGNAASVLVGDFIYTRAFQLVAQLESLKILSIMADATNVLAEGEVQQLMNVN
DPETSEANYMRVIYSKTARLFEVAGQAAAIVAGGTEAQEKALQDYGRYLGTAFQLVDDVLDYSANTQALGKNVGDDLAEG
KPTLPLLHAMRHGNAQQAALIREAIEQGGKREAIDEVLAIMTEHKSLDYAMNRAKEEAQKAVDAIEILPESEYKQALISL
AYLSVDRNY

Sequences:

>Translated_329_residues
MKKQDLMSIDEIQKLADPDMQKVNQNILAQLNSDVPLIGQLGFYIVQGGGKRIRPLIAVLAARSLGFEGSNSITCATFVE
FIHTASLLHDDVVDESDMRRGRATANAEFGNAASVLVGDFIYTRAFQLVAQLESLKILSIMADATNVLAEGEVQQLMNVN
DPETSEANYMRVIYSKTARLFEVAGQAAAIVAGGTEAQEKALQDYGRYLGTAFQLVDDVLDYSANTQALGKNVGDDLAEG
KPTLPLLHAMRHGNAQQAALIREAIEQGGKREAIDEVLAIMTEHKSLDYAMNRAKEEAQKAVDAIEILPESEYKQALISL
AYLSVDRNY
>Mature_329_residues
MKKQDLMSIDEIQKLADPDMQKVNQNILAQLNSDVPLIGQLGFYIVQGGGKRIRPLIAVLAARSLGFEGSNSITCATFVE
FIHTASLLHDDVVDESDMRRGRATANAEFGNAASVLVGDFIYTRAFQLVAQLESLKILSIMADATNVLAEGEVQQLMNVN
DPETSEANYMRVIYSKTARLFEVAGQAAAIVAGGTEAQEKALQDYGRYLGTAFQLVDDVLDYSANTQALGKNVGDDLAEG
KPTLPLLHAMRHGNAQQAALIREAIEQGGKREAIDEVLAIMTEHKSLDYAMNRAKEEAQKAVDAIEILPESEYKQALISL
AYLSVDRNY

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family

Homologues:

Organism=Homo sapiens, GI50659086, Length=322, Percent_Identity=28.5714285714286, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI4758430, Length=269, Percent_Identity=24.5353159851301, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI83700220, Length=269, Percent_Identity=24.5353159851301, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1789578, Length=322, Percent_Identity=64.9068322981366, Blast_Score=440, Evalue=1e-125,
Organism=Escherichia coli, GI1786623, Length=228, Percent_Identity=30.2631578947368, Blast_Score=82, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17505681, Length=333, Percent_Identity=33.6336336336336, Blast_Score=156, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6319475, Length=364, Percent_Identity=31.3186813186813, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6325188, Length=259, Percent_Identity=23.5521235521236, Blast_Score=79, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24651612, Length=302, Percent_Identity=31.4569536423841, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI281365769, Length=245, Percent_Identity=28.1632653061224, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24660002, Length=245, Percent_Identity=28.1632653061224, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPB_HAEIN (P44916)

Other databases:

- EMBL:   L42023
- PIR:   I64160
- RefSeq:   NP_439042.1
- ProteinModelPortal:   P44916
- SMR:   P44916
- GeneID:   949563
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0881
- TIGR:   HI_0881
- HOGENOM:   HBG732667
- OMA:   VQQLMNV
- ProtClustDB:   CLSK870062
- BioCyc:   HINF71421:HI_0881-MONOMER
- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949
- Gene3D:   G3DSA:1.10.600.10
- PANTHER:   PTHR12001

Pfam domain/function: PF00348 polyprenyl_synt; SSF48576 Terpenoid_synth

EC number: =2.5.1.90

Molecular weight: Translated: 35912; Mature: 35912

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00723 POLYPRENYL_SYNTHASE_1; PS00444 POLYPRENYL_SYNTHASE_2

Important sites: BINDING 51-51 BINDING 54-54 BINDING 83-83 BINDING 99-99 BINDING 100-100 BINDING 176-176 BINDING 177-177 BINDING 214-214

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQDLMSIDEIQKLADPDMQKVNQNILAQLNSDVPLIGQLGFYIVQGGGKRIRPLIAVL
CCCHHHCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHCCCEEEEECCCCHHHHHHHHH
AARSLGFEGSNSITCATFVEFIHTASLLHDDVVDESDMRRGRATANAEFGNAASVLVGDF
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHHHHH
IYTRAFQLVAQLESLKILSIMADATNVLAEGEVQQLMNVNDPETSEANYMRVIYSKTARL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
FEVAGQAAAIVAGGTEAQEKALQDYGRYLGTAFQLVDDVLDYSANTQALGKNVGDDLAEG
HHHHCCHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHCC
KPTLPLLHAMRHGNAQQAALIREAIEQGGKREAIDEVLAIMTEHKSLDYAMNRAKEEAQK
CCCHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVDAIEILPESEYKQALISLAYLSVDRNY
HHHHHHHCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKQDLMSIDEIQKLADPDMQKVNQNILAQLNSDVPLIGQLGFYIVQGGGKRIRPLIAVL
CCCHHHCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHCCCEEEEECCCCHHHHHHHHH
AARSLGFEGSNSITCATFVEFIHTASLLHDDVVDESDMRRGRATANAEFGNAASVLVGDF
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHHHHH
IYTRAFQLVAQLESLKILSIMADATNVLAEGEVQQLMNVNDPETSEANYMRVIYSKTARL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
FEVAGQAAAIVAGGTEAQEKALQDYGRYLGTAFQLVDDVLDYSANTQALGKNVGDDLAEG
HHHHCCHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHCC
KPTLPLLHAMRHGNAQQAALIREAIEQGGKREAIDEVLAIMTEHKSLDYAMNRAKEEAQK
CCCHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVDAIEILPESEYKQALISLAYLSVDRNY
HHHHHHHCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023