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The map label for this gene is glnA

Identifier: 16272805

GI number: 16272805

Start: 916620

End: 918038

Strand: Direct

Name: glnA

Synonym: HI0865

Alternate gene names: 16272805

Gene position: 916620-918038 (Clockwise)

Preceding gene: 161350023

Following gene: 16272806

Centisome position: 50.08

GC content: 38.2

Gene sequence:

>1419_bases
ATGCCAAACGCAAATGCAATTGCCAATGTATTTAAGCTAATTGAAGAAAATAATGTGAAATTTGTACTTCTTCGATTTAC
CGATATTAAAGGTAAAGAACATGGTGTTTCTATCCCAGTTAGCCTTGTTGATGAAGATATGTTTGAAGACGGCAAGATGT
TTGATGGTTCCTCTGTTGAAGGATGGAAAACCATTAATAAAGCCGATATGTTGCTTATGCCAATGGCTGAAACGGCAATT
GTTGATCCATTCGCGCAAATTCCAACGCTTTCTATTCGTTGTAGTGTTTATGAACCAACGACAATGCAAAGCTATGATCG
CGATCCGCGCTCTATTGCAATTCGTGCAGAAAATTATATGCGTTCAACAGGTATTGCTGATCAAGCTTTTTTTGGTCCAG
AGCCTGAATTCTTCTTATTTGATGATGTGCGTTTTAATGTATCAATGAACAAAGCATCTTTTTCTATTGATGATATAGAA
GCTGCTTGGAACACGAATAAAAAATATGAAGAAGGCAATAACGCTTATCGCCCATTGAAAAAAGGTGGATATTGTGCCGT
TGCACCGATTGATAGTGCGCACGATATTCGTTCTGAAATGTGCTTGATTTTAGAAGAAATGGGATTAGTCATTGAAGCCC
ATCATCATGAAGTGGCGACTGCAGGGCAAAACGAAATTGCGACTAAATTTAATACGCTAACTTTAAAAGCGGATGAAACT
CAAATTTATAAACATGTCGTACAAAACGTTGCATTAGAGCACGGTAAAACGGCGTGTTTTATGCCCAAACCAATTACTGG
AGATAATGGTTCGGGTATGCACTGTAATATGTCGTTAAGTAAAGACGGAAAAAATATTTTCCAAGGCGATAAATATGCAG
GCCTTTCTGAAACCGCACTTTACTACATCGGCGGTATCATTAAGCACGCTAAAGCCTTAAATGCGTTCACTAATCCAAGT
ACTAATTCATATAAACGTTTAGTGCCTGGTTATGAAGCACCAGTATTGTTGGCTTACTCTGCAAGCAATCGTTCAGCCTC
AATTCGTATCCCTGCTGTAACCAATCCAAAAGCGATTCGCGTAGAAGCACGTTTCCCAGATCCATTGGCAAACCCATATT
TAGCATTTGCTGCATTATTAATGGCAGGTCTTGATGGCGTGGTAAACAAAATCCACCCAGGCGATGCGATGGATAAAAAT
CTTTACGATCTTCCTCCAGAAGAATTAAAAGATATTCCTGCAGTAGCAAGCTCGTTAGAAGAAGCGTTAAATTCATTAGA
AAAAGACTATGAATTCTTAACTCAAGGTGGCGTATTTGCTAAAGATTTTATTGATGCGTTTATCAGTATCAAACGTAAAG
AAGTGGAACGTTTAAATATGGCACCACATCCAGTTGAATTTGAAATGTATTATGCATAA

Upstream 100 bases:

>100_bases
GCTATGACTAGAATAAGAAAAATAAATTTCATGAAAAAATAGCATGGCTACGTTATAATGTGCGCTTATCTTAATAAGAC
TAACCATAGAGGATTTCCTA

Downstream 100 bases:

>100_bases
TGATATATAAACAAGAACAATGCGGTGAAATTTCGCCGTATTTTTTTAAAGGAGTAAATATGACTAAGATTTCTTCTCAA
AAGAATGACTAGATTGATTT

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MPNANAIANVFKLIEENNVKFVLLRFTDIKGKEHGVSIPVSLVDEDMFEDGKMFDGSSVEGWKTINKADMLLMPMAETAI
VDPFAQIPTLSIRCSVYEPTTMQSYDRDPRSIAIRAENYMRSTGIADQAFFGPEPEFFLFDDVRFNVSMNKASFSIDDIE
AAWNTNKKYEEGNNAYRPLKKGGYCAVAPIDSAHDIRSEMCLILEEMGLVIEAHHHEVATAGQNEIATKFNTLTLKADET
QIYKHVVQNVALEHGKTACFMPKPITGDNGSGMHCNMSLSKDGKNIFQGDKYAGLSETALYYIGGIIKHAKALNAFTNPS
TNSYKRLVPGYEAPVLLAYSASNRSASIRIPAVTNPKAIRVEARFPDPLANPYLAFAALLMAGLDGVVNKIHPGDAMDKN
LYDLPPEELKDIPAVASSLEEALNSLEKDYEFLTQGGVFAKDFIDAFISIKRKEVERLNMAPHPVEFEMYYA

Sequences:

>Translated_472_residues
MPNANAIANVFKLIEENNVKFVLLRFTDIKGKEHGVSIPVSLVDEDMFEDGKMFDGSSVEGWKTINKADMLLMPMAETAI
VDPFAQIPTLSIRCSVYEPTTMQSYDRDPRSIAIRAENYMRSTGIADQAFFGPEPEFFLFDDVRFNVSMNKASFSIDDIE
AAWNTNKKYEEGNNAYRPLKKGGYCAVAPIDSAHDIRSEMCLILEEMGLVIEAHHHEVATAGQNEIATKFNTLTLKADET
QIYKHVVQNVALEHGKTACFMPKPITGDNGSGMHCNMSLSKDGKNIFQGDKYAGLSETALYYIGGIIKHAKALNAFTNPS
TNSYKRLVPGYEAPVLLAYSASNRSASIRIPAVTNPKAIRVEARFPDPLANPYLAFAALLMAGLDGVVNKIHPGDAMDKN
LYDLPPEELKDIPAVASSLEEALNSLEKDYEFLTQGGVFAKDFIDAFISIKRKEVERLNMAPHPVEFEMYYA
>Mature_471_residues
PNANAIANVFKLIEENNVKFVLLRFTDIKGKEHGVSIPVSLVDEDMFEDGKMFDGSSVEGWKTINKADMLLMPMAETAIV
DPFAQIPTLSIRCSVYEPTTMQSYDRDPRSIAIRAENYMRSTGIADQAFFGPEPEFFLFDDVRFNVSMNKASFSIDDIEA
AWNTNKKYEEGNNAYRPLKKGGYCAVAPIDSAHDIRSEMCLILEEMGLVIEAHHHEVATAGQNEIATKFNTLTLKADETQ
IYKHVVQNVALEHGKTACFMPKPITGDNGSGMHCNMSLSKDGKNIFQGDKYAGLSETALYYIGGIIKHAKALNAFTNPST
NSYKRLVPGYEAPVLLAYSASNRSASIRIPAVTNPKAIRVEARFPDPLANPYLAFAALLMAGLDGVVNKIHPGDAMDKNL
YDLPPEELKDIPAVASSLEEALNSLEKDYEFLTQGGVFAKDFIDAFISIKRKEVERLNMAPHPVEFEMYYA

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family

Homologues:

Organism=Homo sapiens, GI221307485, Length=500, Percent_Identity=23.2, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1790301, Length=464, Percent_Identity=69.6120689655172, Blast_Score=697, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=175, Percent_Identity=32.5714285714286, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): GLNA_HAEIN (P43794)

Other databases:

- EMBL:   L42023
- PIR:   I64098
- RefSeq:   NP_439025.1
- ProteinModelPortal:   P43794
- SMR:   P43794
- GeneID:   949878
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0865
- NMPDR:   fig|71421.1.peg.833
- TIGR:   HI_0865
- HOGENOM:   HBG553755
- OMA:   IDAFISI
- ProtClustDB:   CLSK870384
- BioCyc:   HINF71421:HI_0865-MONOMER
- BRENDA:   6.3.1.2
- GO:   GO:0005737
- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637
- Gene3D:   G3DSA:3.30.590.10
- TIGRFAMs:   TIGR00653

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N; SSF54368 Gln_synt_beta

EC number: =6.3.1.2

Molecular weight: Translated: 52344; Mature: 52213

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00180 GLNA_1; PS00182 GLNA_ADENYLATION; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNANAIANVFKLIEENNVKFVLLRFTDIKGKEHGVSIPVSLVDEDMFEDGKMFDGSSVE
CCCHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCC
GWKTINKADMLLMPMAETAIVDPFAQIPTLSIRCSVYEPTTMQSYDRDPRSIAIRAENYM
CHHCCCCCCEEEECCHHHHHHCCHHHCCEEEEEEEEECCCCHHHCCCCCCEEEEEEHHHH
RSTGIADQAFFGPEPEFFLFDDVRFNVSMNKASFSIDDIEAAWNTNKKYEEGNNAYRPLK
HHCCCCCCCCCCCCCCEEEEEEEEEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCC
KGGYCAVAPIDSAHDIRSEMCLILEEMGLVIEAHHHEVATAGQNEIATKFNTLTLKADET
CCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCHHHHCCCCHHHEEEEEEEEECCHH
QIYKHVVQNVALEHGKTACFMPKPITGDNGSGMHCNMSLSKDGKNIFQGDKYAGLSETAL
HHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEEEEECCCCCHHHCCCCCCCCCHHHH
YYIGGIIKHAKALNAFTNPSTNSYKRLVPGYEAPVLLAYSASNRSASIRIPAVTNPKAIR
HHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCEEEEEECCCCCEEEEECCCCCCCEEE
VEARFPDPLANPYLAFAALLMAGLDGVVNKIHPGDAMDKNLYDLPPEELKDIPAVASSLE
EEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
EALNSLEKDYEFLTQGGVFAKDFIDAFISIKRKEVERLNMAPHPVEFEMYYA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEC
>Mature Secondary Structure 
PNANAIANVFKLIEENNVKFVLLRFTDIKGKEHGVSIPVSLVDEDMFEDGKMFDGSSVE
CCHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCC
GWKTINKADMLLMPMAETAIVDPFAQIPTLSIRCSVYEPTTMQSYDRDPRSIAIRAENYM
CHHCCCCCCEEEECCHHHHHHCCHHHCCEEEEEEEEECCCCHHHCCCCCCEEEEEEHHHH
RSTGIADQAFFGPEPEFFLFDDVRFNVSMNKASFSIDDIEAAWNTNKKYEEGNNAYRPLK
HHCCCCCCCCCCCCCCEEEEEEEEEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCC
KGGYCAVAPIDSAHDIRSEMCLILEEMGLVIEAHHHEVATAGQNEIATKFNTLTLKADET
CCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCHHHHCCCCHHHEEEEEEEEECCHH
QIYKHVVQNVALEHGKTACFMPKPITGDNGSGMHCNMSLSKDGKNIFQGDKYAGLSETAL
HHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEEEEECCCCCHHHCCCCCCCCCHHHH
YYIGGIIKHAKALNAFTNPSTNSYKRLVPGYEAPVLLAYSASNRSASIRIPAVTNPKAIR
HHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCEEEEEECCCCCEEEEECCCCCCCEEE
VEARFPDPLANPYLAFAALLMAGLDGVVNKIHPGDAMDKNLYDLPPEELKDIPAVASSLE
EEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
EALNSLEKDYEFLTQGGVFAKDFIDAFISIKRKEVERLNMAPHPVEFEMYYA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800