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The map label for this gene is galR

Identifier: 16272762

GI number: 16272762

Start: 872402

End: 873400

Strand: Direct

Name: galR

Synonym: HI0821

Alternate gene names: 16272762

Gene position: 872402-873400 (Clockwise)

Preceding gene: 16272747

Following gene: 30995404

Centisome position: 47.67

GC content: 38.44

Gene sequence:

>999_bases
ATGAGTACTATTCGAGATGTCGCTAAATTAGCCAATGTATCTGTCGCTACCGTATCCCGAGTATTAAATCACTCTCTTTC
TGTTAGTGAAAATACACGCCTTGTGGTGGAACAAGCTATTGCACAGTTAGCTTATCAACCTAATGCAAATGCTCAAGCCC
TTGCTGTGCAAAACACCGATACAATTGGCGTGGTGGTAACCGATGTGACGGATGCCTTTTTTGCGATTTTAGTGAAAGCG
GTGGATAAAGTAGCAGAAGCACATCAAAAAACGATTCTTATCGGTATTGGTTATCATCATGCGGAAAAAGAGCGGGAAGC
AATTAATACCTTATTACGTAAGCGTTGTAGTTCTCTTGTTGTACATTCTAAAGCCTTATCTGATGATGAATTAAGCCATT
ATTTAAATACTGTGCAAGGAATGGTAATCATTAACCGTGTTATTAAAGGTTATGAACATCGTTGTGTCAGTTTAGATAAC
CAAAAAGGCACTTATTTAGCCACGGAAATGCTTATTCGTTATGGGCATCAACATATTGCATATATCGGTTCAAATCACGC
CATTTTTGATGAAGTTGAAAGAAGAAATGGTTATCTTGCCGCATTAAAAGATCACAATTATCCCATCATTGAACAGGCGA
TAACACTCAATAGCCCAGACTTTGAAGGCGGCGAAAAAGCAATGATTGATTTACTTAGTTATAACAAAAATCTTACGGCT
GTCGTTGCTTATAACGATTCAATGGCGGCGGGAGCCATTTCAGTTCTCAATGAAAACAATATCAGCGTACCAAGCCAATT
TTCAATTATTGGTTTTGATGATATGCCGATTGCCCGTTATTTGATCCCAAAACTAACCACAATTCGCTATCCTATTGATT
TAATGGCAACTTATGCGGCTAAATTAGCATTAAGTCTAACTGACGAGAAAATAATTACACCTCCAGTAGTTCAATTTAAC
CCTACTTTGGTACGCCGTTTTTCGGTGGAATCGCGCTAA

Upstream 100 bases:

>100_bases
TATGATTTAGTTCATACTTATGAAAGTAACCGCTTTCAATTTATCGTAAATTATTTATGATCAAAATATAAGATTTATAG
ATTATAACAAGGAAAAAGTT

Downstream 100 bases:

>100_bases
TTTGTGACATGGATCACAAATTCATAATTTTTTATGTAATCATTTTCATTTTTGCAAATTTGCTCATAGATTAATTACGA
TAATTTATTTATGATGTACA

Product: galactose operon repressor

Products: NA

Alternate protein names: Galactose operon repressor

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MSTIRDVAKLANVSVATVSRVLNHSLSVSENTRLVVEQAIAQLAYQPNANAQALAVQNTDTIGVVVTDVTDAFFAILVKA
VDKVAEAHQKTILIGIGYHHAEKEREAINTLLRKRCSSLVVHSKALSDDELSHYLNTVQGMVIINRVIKGYEHRCVSLDN
QKGTYLATEMLIRYGHQHIAYIGSNHAIFDEVERRNGYLAALKDHNYPIIEQAITLNSPDFEGGEKAMIDLLSYNKNLTA
VVAYNDSMAAGAISVLNENNISVPSQFSIIGFDDMPIARYLIPKLTTIRYPIDLMATYAAKLALSLTDEKIITPPVVQFN
PTLVRRFSVESR

Sequences:

>Translated_332_residues
MSTIRDVAKLANVSVATVSRVLNHSLSVSENTRLVVEQAIAQLAYQPNANAQALAVQNTDTIGVVVTDVTDAFFAILVKA
VDKVAEAHQKTILIGIGYHHAEKEREAINTLLRKRCSSLVVHSKALSDDELSHYLNTVQGMVIINRVIKGYEHRCVSLDN
QKGTYLATEMLIRYGHQHIAYIGSNHAIFDEVERRNGYLAALKDHNYPIIEQAITLNSPDFEGGEKAMIDLLSYNKNLTA
VVAYNDSMAAGAISVLNENNISVPSQFSIIGFDDMPIARYLIPKLTTIRYPIDLMATYAAKLALSLTDEKIITPPVVQFN
PTLVRRFSVESR
>Mature_331_residues
STIRDVAKLANVSVATVSRVLNHSLSVSENTRLVVEQAIAQLAYQPNANAQALAVQNTDTIGVVVTDVTDAFFAILVKAV
DKVAEAHQKTILIGIGYHHAEKEREAINTLLRKRCSSLVVHSKALSDDELSHYLNTVQGMVIINRVIKGYEHRCVSLDNQ
KGTYLATEMLIRYGHQHIAYIGSNHAIFDEVERRNGYLAALKDHNYPIIEQAITLNSPDFEGGEKAMIDLLSYNKNLTAV
VAYNDSMAAGAISVLNENNISVPSQFSIIGFDDMPIARYLIPKLTTIRYPIDLMATYAAKLALSLTDEKIITPPVVQFNP
TLVRRFSVESR

Specific function: Repressor of the galactose operon. Binds galactose as an inducer

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1788474, Length=332, Percent_Identity=57.5301204819277, Blast_Score=383, Evalue=1e-108,
Organism=Escherichia coli, GI1789202, Length=331, Percent_Identity=51.6616314199396, Blast_Score=340, Evalue=1e-94,
Organism=Escherichia coli, GI1789068, Length=332, Percent_Identity=34.0361445783133, Blast_Score=184, Evalue=5e-48,
Organism=Escherichia coli, GI1787948, Length=336, Percent_Identity=32.4404761904762, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1790369, Length=290, Percent_Identity=31.7241379310345, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1790194, Length=330, Percent_Identity=30.3030303030303, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1787906, Length=309, Percent_Identity=29.4498381877023, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1787580, Length=318, Percent_Identity=29.559748427673, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1790715, Length=312, Percent_Identity=26.6025641025641, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1786540, Length=328, Percent_Identity=28.3536585365854, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI48994940, Length=327, Percent_Identity=25.3822629969419, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790689, Length=289, Percent_Identity=24.5674740484429, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1786268, Length=326, Percent_Identity=21.1656441717791, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GALR_HAEIN (P31766)

Other databases:

- EMBL:   X65934
- EMBL:   L42023
- PIR:   F64096
- RefSeq:   NP_438981.1
- ProteinModelPortal:   P31766
- SMR:   P31766
- GeneID:   949832
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0821
- NMPDR:   fig|71421.1.peg.789
- TIGR:   HI_0821
- HOGENOM:   HBG753640
- OMA:   NENNIRV
- ProtClustDB:   CLSK789635
- BioCyc:   HINF71421:HI_0821-MONOMER
- GO:   GO:0005622
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761
- PRINTS:   PR00036
- SMART:   SM00354

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1; SSF47413 Lambda_like_DNA

EC number: NA

Molecular weight: Translated: 36709; Mature: 36577

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS00356 HTH_LACI_1; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIRDVAKLANVSVATVSRVLNHSLSVSENTRLVVEQAIAQLAYQPNANAQALAVQNTD
CCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCC
TIGVVVTDVTDAFFAILVKAVDKVAEAHQKTILIGIGYHHAEKEREAINTLLRKRCSSLV
EEEEEEECHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHH
VHSKALSDDELSHYLNTVQGMVIINRVIKGYEHRCVSLDNQKGTYLATEMLIRYGHQHIA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCEEE
YIGSNHAIFDEVERRNGYLAALKDHNYPIIEQAITLNSPDFEGGEKAMIDLLSYNKNLTA
EECCCCHHHHHHHHCCCEEEEEECCCCCHHHHEEECCCCCCCCCHHHHHHHHHCCCCEEE
VVAYNDSMAAGAISVLNENNISVPSQFSIIGFDDMPIARYLIPKLTTIRYPIDLMATYAA
EEEECCCCCCCCCEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHEECCHHHHHHHHH
KLALSLTDEKIITPPVVQFNPTLVRRFSVESR
HHHEEECCCCCCCCCCEECCHHHHEEECCCCC
>Mature Secondary Structure 
STIRDVAKLANVSVATVSRVLNHSLSVSENTRLVVEQAIAQLAYQPNANAQALAVQNTD
CHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCC
TIGVVVTDVTDAFFAILVKAVDKVAEAHQKTILIGIGYHHAEKEREAINTLLRKRCSSLV
EEEEEEECHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHH
VHSKALSDDELSHYLNTVQGMVIINRVIKGYEHRCVSLDNQKGTYLATEMLIRYGHQHIA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCEEE
YIGSNHAIFDEVERRNGYLAALKDHNYPIIEQAITLNSPDFEGGEKAMIDLLSYNKNLTA
EECCCCHHHHHHHHCCCEEEEEECCCCCHHHHEEECCCCCCCCCHHHHHHHHHCCCCEEE
VVAYNDSMAAGAISVLNENNISVPSQFSIIGFDDMPIARYLIPKLTTIRYPIDLMATYAA
EEEECCCCCCCCCEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHEECCHHHHHHHHH
KLALSLTDEKIITPPVVQFNPTLVRRFSVESR
HHHEEECCCCCCCCCCEECCHHHHEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1282642; 7542800