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The map label for this gene is mglB

Identifier: 30995404

GI number: 30995404

Start: 873545

End: 874540

Strand: Direct

Name: mglB

Synonym: HI0822

Alternate gene names: 30995404

Gene position: 873545-874540 (Clockwise)

Preceding gene: 16272762

Following gene: 16272764

Centisome position: 47.73

GC content: 37.15

Gene sequence:

>996_bases
ATGAAAAAAACAGCCGTGTTAAGTACCGTCGCTTTCGCTATTGCATTAGGTTCAGCATCTGCAAGTTTTGCTGCAGATAA
TCGTATTGGGGTCACGATTTATAAATATGATGATAACTTTATGTCTTTAATGCGCAAAGAAATTGATAAAGAAGCTAAAG
TAGTCGGTGGCATTAAACTCTTAATGAATGACTCTCAAAATGCACAATCTATTCAAAATGACCAAGTTGATATCTTACTC
TCTAAAGGCGTGAAAGCACTTGCGATTAACTTGGTGGACCCAGCGGCAGCTCCAACTATTATTGGCAAGGCAAAATCAGA
TAATATTCCTGTCGTATTCTTTAACAAAGACCCTGGTGCAAAAGCTATCGGTTCTTATGAACAGGCTTACTATGTAGGTA
CTGATCCAAAAGAATCAGGCTTAATTCAAGGGGATTTAATTGCAAAACAATGGAAAGCGAATCCAGCTCTTGACTTAAAT
AAAGATGGCAAAATCCAATTTGTATTATTAAAAGGCGAACCTGGTCACCCAGATGCGGAAGTGCGTACAAAATATGTTAT
AGAAGAACTTAATGCGAAAGGTATCCAAACTGAACAATTATTTATTGATACTGGTATGTGGGATGCTGCAATGGCGAAAG
ATAAAGTAGATGCTTGGTTATCTAGCTCTAAAGCGAACGACATTGAAGTCATTATTTCTAACAACGATGGTATGGCATTA
GGTGCGTTAGAAGCAACAAAAGCACACGGTAAAAAATTACCTATCTTTGGTGTGGATGCGTTACCAGAAGCATTACAATT
GATTAGTAAAGGCGAACTTGCGGGTACAGTATTAAACGATAGTGTAAACCAAGGTAAAGCAGTTGTTCAATTATCTAACA
ATTTAGCACAAGGTAAATCTGCAACTGAAGGTACAAAATGGGAATTAAAAGATCGTGTTGTACGCATTCCTTATGTTGGT
GTGGATAAAGACAACTTAGGTGACTTCTTAAAATAA

Upstream 100 bases:

>100_bases
TTCATTTTTGCAAATTTGCTCATAGATTAATTACGATAATTTATTTATGATGTACACAACCTTATCTATCCACATCAACC
TTCCAAATAGGAGTGTTATT

Downstream 100 bases:

>100_bases
TTTCCCATCAATTTTTGTTGTAATCTAAGGGGAAGTGGATCTTCCCCTTTTTAAAGAGGTTGGATATGACAGCTCAAACT
CAATGTCAAGACAGCCAAGT

Product: galactose ABC transporter periplasmic-binding protein

Products: ADP; phosphate; beta-D-galactose [Cytoplasm] [C]

Alternate protein names: GBP; D-galactose/ D-glucose-binding protein; GGBP

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MKKTAVLSTVAFAIALGSASASFAADNRIGVTIYKYDDNFMSLMRKEIDKEAKVVGGIKLLMNDSQNAQSIQNDQVDILL
SKGVKALAINLVDPAAAPTIIGKAKSDNIPVVFFNKDPGAKAIGSYEQAYYVGTDPKESGLIQGDLIAKQWKANPALDLN
KDGKIQFVLLKGEPGHPDAEVRTKYVIEELNAKGIQTEQLFIDTGMWDAAMAKDKVDAWLSSSKANDIEVIISNNDGMAL
GALEATKAHGKKLPIFGVDALPEALQLISKGELAGTVLNDSVNQGKAVVQLSNNLAQGKSATEGTKWELKDRVVRIPYVG
VDKDNLGDFLK

Sequences:

>Translated_331_residues
MKKTAVLSTVAFAIALGSASASFAADNRIGVTIYKYDDNFMSLMRKEIDKEAKVVGGIKLLMNDSQNAQSIQNDQVDILL
SKGVKALAINLVDPAAAPTIIGKAKSDNIPVVFFNKDPGAKAIGSYEQAYYVGTDPKESGLIQGDLIAKQWKANPALDLN
KDGKIQFVLLKGEPGHPDAEVRTKYVIEELNAKGIQTEQLFIDTGMWDAAMAKDKVDAWLSSSKANDIEVIISNNDGMAL
GALEATKAHGKKLPIFGVDALPEALQLISKGELAGTVLNDSVNQGKAVVQLSNNLAQGKSATEGTKWELKDRVVRIPYVG
VDKDNLGDFLK
>Mature_331_residues
MKKTAVLSTVAFAIALGSASASFAADNRIGVTIYKYDDNFMSLMRKEIDKEAKVVGGIKLLMNDSQNAQSIQNDQVDILL
SKGVKALAINLVDPAAAPTIIGKAKSDNIPVVFFNKDPGAKAIGSYEQAYYVGTDPKESGLIQGDLIAKQWKANPALDLN
KDGKIQFVLLKGEPGHPDAEVRTKYVIEELNAKGIQTEQLFIDTGMWDAAMAKDKVDAWLSSSKANDIEVIISNNDGMAL
GALEATKAHGKKLPIFGVDALPEALQLISKGELAGTVLNDSVNQGKAVVQLSNNLAQGKSATEGTKWELKDRVVRIPYVG
VDKDNLGDFLK

Specific function: This protein is involved in the active transport of galactose and glucose

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family

Homologues:

Organism=Escherichia coli, GI1788473, Length=331, Percent_Identity=67.3716012084592, Blast_Score=453, Evalue=1e-129,

Paralogues:

None

Copy number: 4720 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1320 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): DGAL_HAEIN (P44883)

Other databases:

- EMBL:   L42023
- PIR:   G64096
- RefSeq:   NP_438982.2
- ProteinModelPortal:   P44883
- SMR:   P44883
- GeneID:   949834
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0822
- NMPDR:   fig|71421.1.peg.790
- TIGR:   HI_0822
- HOGENOM:   HBG617058
- OMA:   KIENKVV
- ProtClustDB:   PRK15395
- BioCyc:   HINF71421:HI_0822-MONOMER
- InterPro:   IPR001761

Pfam domain/function: PF00532 Peripla_BP_1

EC number: NA

Molecular weight: Translated: 35518; Mature: 35518

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTAVLSTVAFAIALGSASASFAADNRIGVTIYKYDDNFMSLMRKEIDKEAKVVGGIKL
CCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHCCEEE
LMNDSQNAQSIQNDQVDILLSKGVKALAINLVDPAAAPTIIGKAKSDNIPVVFFNKDPGA
EECCCCCHHHHCCCCEEEEECCCCEEEEEEEECCCCCCEEEECCCCCCCCEEEECCCCCC
KAIGSYEQAYYVGTDPKESGLIQGDLIAKQWKANPALDLNKDGKIQFVLLKGEPGHPDAE
HHHCCCCCEEEECCCCCCCCCEEHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCHH
VRTKYVIEELNAKGIQTEQLFIDTGMWDAAMAKDKVDAWLSSSKANDIEVIISNNDGMAL
HHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEE
GALEATKAHGKKLPIFGVDALPEALQLISKGELAGTVLNDSVNQGKAVVQLSNNLAQGKS
EEEHHHHHCCCCCCEEECHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEECCCHHCCCC
ATEGTKWELKDRVVRIPYVGVDKDNLGDFLK
CCCCCCEEECCCEEEEEEEECCCCCCHHHCC
>Mature Secondary Structure
MKKTAVLSTVAFAIALGSASASFAADNRIGVTIYKYDDNFMSLMRKEIDKEAKVVGGIKL
CCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHCCEEE
LMNDSQNAQSIQNDQVDILLSKGVKALAINLVDPAAAPTIIGKAKSDNIPVVFFNKDPGA
EECCCCCHHHHCCCCEEEEECCCCEEEEEEEECCCCCCEEEECCCCCCCCEEEECCCCCC
KAIGSYEQAYYVGTDPKESGLIQGDLIAKQWKANPALDLNKDGKIQFVLLKGEPGHPDAE
HHHCCCCCEEEECCCCCCCCCEEHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCHH
VRTKYVIEELNAKGIQTEQLFIDTGMWDAAMAKDKVDAWLSSSKANDIEVIISNNDGMAL
HHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEE
GALEATKAHGKKLPIFGVDALPEALQLISKGELAGTVLNDSVNQGKAVVQLSNNLAQGKS
EEEHHHHHCCCCCCEEECHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEECCCHHCCCC
ATEGTKWELKDRVVRIPYVGVDKDNLGDFLK
CCCCCCEEECCCEEEEEEEECCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; beta-D-galactose [Periplasm]; H2O [C]

Specific reaction: ATP + beta-D-galactose [Periplasm] + H2O = ADP + phosphate + beta-D-galactose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023