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The map label for this gene is ilvG [C]

Identifier: 16272678

GI number: 16272678

Start: 791772

End: 792569

Strand: Direct

Name: ilvG [C]

Synonym: HI0737

Alternate gene names: 16272678

Gene position: 791772-792569 (Clockwise)

Preceding gene: 16272676

Following gene: 16272679

Centisome position: 43.26

GC content: 43.48

Gene sequence:

>798_bases
ATGACAGGCGCACAACTCATTATGGCGTGCTTGAAAGCACACCATGTCACGACTCTTTTCGGCTATCCTGGTGGCGCAAT
TATGCCAACCTACGATGCCCTTTATGACGCAGGCTTAGATCACTTACTTTGTCGTAATGAACAAGGGACTGCAATGGCTG
CGATTGGCTATGCTCGCTCCACGGGAAAAGTGGGCGTCTGTATTGCAACATCAGGCCCTGGTGCCACTAATTTAGTTATT
GGATTAGGCGATGCTATGATGGACTCCATTCCAGTTGTCACCATTACTGGTCAAGTTGCCTCTCCATTAATCGGCACAGA
TGCGTTCCAAGAAGCGGATGTTCTAGGACTTTCCCTTGCTTGCACTAAACACAGCTTTATTGTGCAAAGTGCGGATGTTG
CCCTACAAGGGGATTTAATCCAAGCGCTTAATGCGCTCAAACAAGATCTTGATATTGAACCTTGGCGTGAACAAATCAGA
AATTTCAAAGCAAAATTAGATTTCACTTATGTTGAAAATCAAGGCAATCGCCCGATTGATCCTTGGGCATTACTCAACAG
CCTATCTAATCGTAAACCAAATAATGCGATAATTTGTACTGATGTGGGTCAGCATCAAATGTGGTTAGTGCAACACATTT
TACGCGTAGCACGTCACTGCGGATTTACCGTTACAACAATGGAAATGACACTCATTGAAACCCAAGTGCGGTTAAAAATC
ACTGTAAAATCTGACCGCACTTTAGATCTATTAGTGAATCAGTTGGTTAAGCTGCCGGATGTTTTAATGGTTAATTAA

Upstream 100 bases:

>100_bases
CTTTTTTTCGTTTTTTTCTCTCCAAGCCCTACCAATGACTCGAAATTTCGCTACAATGCTGAACTTATCCCCAAAATAAA
AACAACAGGAAACAAACACC

Downstream 100 bases:

>100_bases
GAATATTTTATCAATTAACGAAGAAATTAATCATACAAACGAACCCTAAATTTTACTCGGAGAGATTATTTATGCCCAAA
CTACGTTCAGCGACCAGCAC

Product: acetohydroxy acid synthase II

Products: 2-acetolactate; CO2

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MTGAQLIMACLKAHHVTTLFGYPGGAIMPTYDALYDAGLDHLLCRNEQGTAMAAIGYARSTGKVGVCIATSGPGATNLVI
GLGDAMMDSIPVVTITGQVASPLIGTDAFQEADVLGLSLACTKHSFIVQSADVALQGDLIQALNALKQDLDIEPWREQIR
NFKAKLDFTYVENQGNRPIDPWALLNSLSNRKPNNAIICTDVGQHQMWLVQHILRVARHCGFTVTTMEMTLIETQVRLKI
TVKSDRTLDLLVNQLVKLPDVLMVN

Sequences:

>Translated_265_residues
MTGAQLIMACLKAHHVTTLFGYPGGAIMPTYDALYDAGLDHLLCRNEQGTAMAAIGYARSTGKVGVCIATSGPGATNLVI
GLGDAMMDSIPVVTITGQVASPLIGTDAFQEADVLGLSLACTKHSFIVQSADVALQGDLIQALNALKQDLDIEPWREQIR
NFKAKLDFTYVENQGNRPIDPWALLNSLSNRKPNNAIICTDVGQHQMWLVQHILRVARHCGFTVTTMEMTLIETQVRLKI
TVKSDRTLDLLVNQLVKLPDVLMVN
>Mature_264_residues
TGAQLIMACLKAHHVTTLFGYPGGAIMPTYDALYDAGLDHLLCRNEQGTAMAAIGYARSTGKVGVCIATSGPGATNLVIG
LGDAMMDSIPVVTITGQVASPLIGTDAFQEADVLGLSLACTKHSFIVQSADVALQGDLIQALNALKQDLDIEPWREQIRN
FKAKLDFTYVENQGNRPIDPWALLNSLSNRKPNNAIICTDVGQHQMWLVQHILRVARHCGFTVTTMEMTLIETQVRLKIT
VKSDRTLDLLVNQLVKLPDVLMVN

Specific function: Truncated acetolactase synthase; no longer catalytically active

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family

Homologues:

Organism=Escherichia coli, GI87081685, Length=133, Percent_Identity=50.3759398496241, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI1790104, Length=130, Percent_Identity=50, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1786717, Length=133, Percent_Identity=36.8421052631579, Blast_Score=92, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323755, Length=133, Percent_Identity=50.3759398496241, Blast_Score=143, Evalue=3e-35,
Organism=Drosophila melanogaster, GI19922626, Length=121, Percent_Identity=33.0578512396694, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y737_HAEIN (O05031)

Other databases:

- EMBL:   L42023
- PIR:   F64089
- RefSeq:   NP_438896.1
- ProteinModelPortal:   O05031
- SMR:   O05031
- GeneID:   949765
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0737
- NMPDR:   fig|71421.1.peg.705
- TIGR:   HI_0737
- HOGENOM:   HBG495042
- OMA:   FTYVENQ
- ProtClustDB:   CLSK808447
- BioCyc:   HINF71421:HI_0737-MONOMER
- InterPro:   IPR012001

Pfam domain/function: PF02776 TPP_enzyme_N

EC number: 2.2.1.6

Molecular weight: Translated: 28785; Mature: 28654

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: BINDING 47-47

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGAQLIMACLKAHHVTTLFGYPGGAIMPTYDALYDAGLDHLLCRNEQGTAMAAIGYARS
CCHHHHHHHHHHHHCEEEEEECCCCCCCCCHHHHHHCCCHHHEECCCCCCEEEEEEEECC
TGKVGVCIATSGPGATNLVIGLGDAMMDSIPVVTITGQVASPLIGTDAFQEADVLGLSLA
CCCEEEEEEECCCCCCEEEEECCHHHHCCCCEEEEECCHHCCCCCCCCHHCCHHHEEEEE
CTKHSFIVQSADVALQGDLIQALNALKQDLDIEPWREQIRNFKAKLDFTYVENQGNRPID
ECCCEEEEECCCEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEEEECCCCCCCC
PWALLNSLSNRKPNNAIICTDVGQHQMWLVQHILRVARHCGFTVTTMEMTLIETQVRLKI
HHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEEEEEEE
TVKSDRTLDLLVNQLVKLPDVLMVN
EEECCCHHHHHHHHHHCCCCEEEEC
>Mature Secondary Structure 
TGAQLIMACLKAHHVTTLFGYPGGAIMPTYDALYDAGLDHLLCRNEQGTAMAAIGYARS
CHHHHHHHHHHHHCEEEEEECCCCCCCCCHHHHHHCCCHHHEECCCCCCEEEEEEEECC
TGKVGVCIATSGPGATNLVIGLGDAMMDSIPVVTITGQVASPLIGTDAFQEADVLGLSLA
CCCEEEEEEECCCCCCEEEEECCHHHHCCCCEEEEECCHHCCCCCCCCHHCCHHHEEEEE
CTKHSFIVQSADVALQGDLIQALNALKQDLDIEPWREQIRNFKAKLDFTYVENQGNRPID
ECCCEEEEECCCEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEEEECCCCCCCC
PWALLNSLSNRKPNNAIICTDVGQHQMWLVQHILRVARHCGFTVTTMEMTLIETQVRLKI
HHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEEEEEEE
TVKSDRTLDLLVNQLVKLPDVLMVN
EEECCCHHHHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} [C]

Substrates: pyruvate

Specific reaction: 2 pyruvate = 2-acetolactate + CO2

General reaction: C-C bond formation; decarboxylation [C]

Inhibitor: @OXOB02.txt*2-oxobutanoate!; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800