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The map label for this gene is lptD [H]

Identifier: 16272671

GI number: 16272671

Start: 784372

End: 786720

Strand: Reverse

Name: lptD [H]

Synonym: HI0730

Alternate gene names: 16272671

Gene position: 786720-784372 (Counterclockwise)

Preceding gene: 16272673

Following gene: 16272667

Centisome position: 42.99

GC content: 36.95

Gene sequence:

>2349_bases
ATGAATAAAAAACACACTTTAATTTCACTTGCTATTTTGACCGCACTTTATAGCCAACAAAGTTTGGCAGATTTGCATGA
ACAATGTTTAATGGGCGTGCCAAAATTTAGCGGTGAAGTCGTGACGGGTGATGTGAATGCTCTGCCTGTCTATATTGAAG
CGGATAACGCAGAAATTAATCAGCCAAATGATGCGACCTATCAAGGTAATGTAGATTTGAAACAAGGCAATCGCCATTTG
TTAGCACAATCCGTGCAGGTCAAACAATCTGGAAACCAATCAACACCTTTACGCATGGCTTATGTCCGCAATGGATTTGA
TTATAAAGATAATCAGATCAATATGTTGGGTAAAGATGCAGAGTTTAATTTAGATAGTCACGATGGTAATTTAACAAATT
CAGAATATGAATTTGTCGGTCGTCAAGGACGTGGTAAAGCTGATAATATAACACTGCATAATAATTATCGTGTAATGAAG
AATGCAACCTTTACGTCATGTTTGCACGGCGACAATGCTTGGGCAGTAGATGCGTCAGAAATTCGTCAATACGTGAAAGA
AGAATATGCTGAAATGTGGCATGCTCGCTTTAAAATACACGGTGTTCCCGTCTTTTATACCCCTTACTTACAATTACCCA
TTGGAGATCGTCGTCGTTCTGGTTTATTGATTCCAAGCGCGGGAACATCCAGCCAAGATGGTCTTTGGTATGCACAACCA
ATTTATTGGAATATCGCGCCTAATTATGATCTTACTTTCACCCCGAAATATATGTCTCGCCGTGGTTGGCAAGCAAATGG
GGAATTTCGCTATTTAACCTCTATTGGTGAAGGTAAAGTGGCAGGAGAATATTTAGGTAAGGTTCGTTACTCTGAATATG
CTAGTGATAATCGAAAACGTCATTTGTTCTATTGGAATCACAATTCTAGCTTTTTACAAAATTGGCGTCTAAATATTAAT
TACACCCGAGTAAGCGATAAACGTTATTTCAACGACTTTGATTCTATTTATGGACGCAGTACCGATGGTTATGCGAACCA
ATATGCTCGCATAGCTTATTATCAACCAAATTATAATTTTTCTCTTTCTGCACATCAGTTCCAAATTTTTGATGATATTG
TAAACATCGGCCCTTACCGTGCAGTACCACAATTAGATTTCAACTATCACAAATATGATTTAGCGAATGGATGGTTAAAT
TTCAAATTACATTCACAAGCAGTTCGTTTTGATAATGACAGTAAATTGATGCCAACTGCATGGCGTTTCCATGCAGAACC
TAGCCTAAATTCTTTAATGTCAAATAAATACGGCAGTTTGAATATTGAAACGAAACTTTATGCGACTCGTTACGAGCAGA
AAAAAGGTTCAGGAAAAAATGCAGAGGACGTGCAAAAAACGGTAAATCGAGTTATTCCACAATTTAAAGTTGATTTACAA
AGTGTATTGGCACGCGATATAACGTTCTTAAAAGAGTATACACAAACCTTCGAACCGCACGTGCAGTATTTATATCGTCC
TTACCGAAATCAAAGCAATATTGGTTCAACACTTAATAATGATTATTTGGGATTTGGTTACGATTCAGCATTAGTACAGC
AAGATTACTATTCGCTATTCCGTGATCGCCGTTATAGTGGTTTAGATCGAATTTCATCGGCAAATCAAGTTACCTTGGGT
GGCACAACGCGTTTTTATGATATTGCTGGCGAAGAACGATTTAATTTATCTGCAGGTCAAATTTATTACTTAAGTAATTC
TCGTATTGATGAAAACCCTGCGAATAAAACACCAACTTCATCATCTGCTTGGGCATTAGAATCTAATTGGAAAATAAGTA
ATAAATGGTATTGGCGTGGTAGCTATCAATTTGATACTCATACCAATTCGACATCATTAGCGAATACATCACTAGAATAT
AATCCTGAAAAAAATAATTTAATTCAGTTAAATTATCGATATGCGAACCAAGAATATATTGACCAAAACTTAGGTAAATC
GGCTAACGCCTATCAACAAGATATCCAACAAGTGGGATTAGTTGTAGGCTGGGAAATTGCCAATAATTGGGCTGTAGTAG
GACGCTATTATCAAGATCTCGCCTTACAAAAACCAGTAGAACAATATCTAGGCGTTCAATATAACAGTTGCTGTTGGGCT
GCAAGTGTTGGTGTAAAACGCAATGTCACAAACCACCAAAACCAAACACGTAATGAAATTGTTTATGATAATAGCATTGG
CATAACCTTAGAATTAAGGGGGTTAGGCAGCAATGACCATCAAAGCGGTATTCAAGAGATGTTAGAAAAAGGAAAACTAC
CTTATATTCGAGCATTTAGTTTGGATTAA

Upstream 100 bases:

>100_bases
CCCAGTGGATATTCTATTAATCACTTCTTGAAAAATATTACTAGCCTTGCACTTTTTCCAGTATAATCTGACCAATTTTC
TGACAAATTAAAACAAGACG

Downstream 100 bases:

>100_bases
ACATTGTTCAATATAAACGTTAGATCAACAAAAAGAGCGGCTATTTTTAACCGCTCTTTTGTCACAATATACAAAATAAA
ATACTGATTAGAGATTACCT

Product: organic solvent tolerance protein

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 782; Mature: 782

Protein sequence:

>782_residues
MNKKHTLISLAILTALYSQQSLADLHEQCLMGVPKFSGEVVTGDVNALPVYIEADNAEINQPNDATYQGNVDLKQGNRHL
LAQSVQVKQSGNQSTPLRMAYVRNGFDYKDNQINMLGKDAEFNLDSHDGNLTNSEYEFVGRQGRGKADNITLHNNYRVMK
NATFTSCLHGDNAWAVDASEIRQYVKEEYAEMWHARFKIHGVPVFYTPYLQLPIGDRRRSGLLIPSAGTSSQDGLWYAQP
IYWNIAPNYDLTFTPKYMSRRGWQANGEFRYLTSIGEGKVAGEYLGKVRYSEYASDNRKRHLFYWNHNSSFLQNWRLNIN
YTRVSDKRYFNDFDSIYGRSTDGYANQYARIAYYQPNYNFSLSAHQFQIFDDIVNIGPYRAVPQLDFNYHKYDLANGWLN
FKLHSQAVRFDNDSKLMPTAWRFHAEPSLNSLMSNKYGSLNIETKLYATRYEQKKGSGKNAEDVQKTVNRVIPQFKVDLQ
SVLARDITFLKEYTQTFEPHVQYLYRPYRNQSNIGSTLNNDYLGFGYDSALVQQDYYSLFRDRRYSGLDRISSANQVTLG
GTTRFYDIAGEERFNLSAGQIYYLSNSRIDENPANKTPTSSSAWALESNWKISNKWYWRGSYQFDTHTNSTSLANTSLEY
NPEKNNLIQLNYRYANQEYIDQNLGKSANAYQQDIQQVGLVVGWEIANNWAVVGRYYQDLALQKPVEQYLGVQYNSCCWA
ASVGVKRNVTNHQNQTRNEIVYDNSIGITLELRGLGSNDHQSGIQEMLEKGKLPYIRAFSLD

Sequences:

>Translated_782_residues
MNKKHTLISLAILTALYSQQSLADLHEQCLMGVPKFSGEVVTGDVNALPVYIEADNAEINQPNDATYQGNVDLKQGNRHL
LAQSVQVKQSGNQSTPLRMAYVRNGFDYKDNQINMLGKDAEFNLDSHDGNLTNSEYEFVGRQGRGKADNITLHNNYRVMK
NATFTSCLHGDNAWAVDASEIRQYVKEEYAEMWHARFKIHGVPVFYTPYLQLPIGDRRRSGLLIPSAGTSSQDGLWYAQP
IYWNIAPNYDLTFTPKYMSRRGWQANGEFRYLTSIGEGKVAGEYLGKVRYSEYASDNRKRHLFYWNHNSSFLQNWRLNIN
YTRVSDKRYFNDFDSIYGRSTDGYANQYARIAYYQPNYNFSLSAHQFQIFDDIVNIGPYRAVPQLDFNYHKYDLANGWLN
FKLHSQAVRFDNDSKLMPTAWRFHAEPSLNSLMSNKYGSLNIETKLYATRYEQKKGSGKNAEDVQKTVNRVIPQFKVDLQ
SVLARDITFLKEYTQTFEPHVQYLYRPYRNQSNIGSTLNNDYLGFGYDSALVQQDYYSLFRDRRYSGLDRISSANQVTLG
GTTRFYDIAGEERFNLSAGQIYYLSNSRIDENPANKTPTSSSAWALESNWKISNKWYWRGSYQFDTHTNSTSLANTSLEY
NPEKNNLIQLNYRYANQEYIDQNLGKSANAYQQDIQQVGLVVGWEIANNWAVVGRYYQDLALQKPVEQYLGVQYNSCCWA
ASVGVKRNVTNHQNQTRNEIVYDNSIGITLELRGLGSNDHQSGIQEMLEKGKLPYIRAFSLD
>Mature_782_residues
MNKKHTLISLAILTALYSQQSLADLHEQCLMGVPKFSGEVVTGDVNALPVYIEADNAEINQPNDATYQGNVDLKQGNRHL
LAQSVQVKQSGNQSTPLRMAYVRNGFDYKDNQINMLGKDAEFNLDSHDGNLTNSEYEFVGRQGRGKADNITLHNNYRVMK
NATFTSCLHGDNAWAVDASEIRQYVKEEYAEMWHARFKIHGVPVFYTPYLQLPIGDRRRSGLLIPSAGTSSQDGLWYAQP
IYWNIAPNYDLTFTPKYMSRRGWQANGEFRYLTSIGEGKVAGEYLGKVRYSEYASDNRKRHLFYWNHNSSFLQNWRLNIN
YTRVSDKRYFNDFDSIYGRSTDGYANQYARIAYYQPNYNFSLSAHQFQIFDDIVNIGPYRAVPQLDFNYHKYDLANGWLN
FKLHSQAVRFDNDSKLMPTAWRFHAEPSLNSLMSNKYGSLNIETKLYATRYEQKKGSGKNAEDVQKTVNRVIPQFKVDLQ
SVLARDITFLKEYTQTFEPHVQYLYRPYRNQSNIGSTLNNDYLGFGYDSALVQQDYYSLFRDRRYSGLDRISSANQVTLG
GTTRFYDIAGEERFNLSAGQIYYLSNSRIDENPANKTPTSSSAWALESNWKISNKWYWRGSYQFDTHTNSTSLANTSLEY
NPEKNNLIQLNYRYANQEYIDQNLGKSANAYQQDIQQVGLVVGWEIANNWAVVGRYYQDLALQKPVEQYLGVQYNSCCWA
ASVGVKRNVTNHQNQTRNEIVYDNSIGITLELRGLGSNDHQSGIQEMLEKGKLPYIRAFSLD

Specific function: Involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

Organism=Escherichia coli, GI1786239, Length=791, Percent_Identity=47.0290771175727, Blast_Score=677, Evalue=0.0,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543 [H]

Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 90085; Mature: 90085

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKHTLISLAILTALYSQQSLADLHEQCLMGVPKFSGEVVTGDVNALPVYIEADNAEIN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEEECCCCCCC
QPNDATYQGNVDLKQGNRHLLAQSVQVKQSGNQSTPLRMAYVRNGFDYKDNQINMLGKDA
CCCCCEEECCEECCCCCCEEHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEEEECCCC
EFNLDSHDGNLTNSEYEFVGRQGRGKADNITLHNNYRVMKNATFTSCLHGDNAWAVDASE
CCCCCCCCCCCCCCHHHEECCCCCCCCCEEEEECCEEEEECCCEEHEECCCCCEEECHHH
IRQYVKEEYAEMWHARFKIHGVPVFYTPYLQLPIGDRRRSGLLIPSAGTSSQDGLWYAQP
HHHHHHHHHHHHHHHEEEEECEEEEECCEEEECCCCCCCCCEEEECCCCCCCCCEEEECC
IYWNIAPNYDLTFTPKYMSRRGWQANGEFRYLTSIGEGKVAGEYLGKVRYSEYASDNRKR
EEEEECCCCCEEECHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEE
HLFYWNHNSSFLQNWRLNINYTRVSDKRYFNDFDSIYGRSTDGYANQYARIAYYQPNYNF
EEEEEECCCHHHHCEEEEEEEEEECCCHHHCCHHHHCCCCCCCCCCCEEEEEEECCCCCE
SLSAHQFQIFDDIVNIGPYRAVPQLDFNYHKYDLANGWLNFKLHSQAVRFDNDSKLMPTA
EECCCCEEHHHHHHCCCCCCCCCCCCCCCEEEECCCCEEEEEEECEEEEECCCCCCCCCC
WRFHAEPSLNSLMSNKYGSLNIETKLYATRYEQKKGSGKNAEDVQKTVNRVIPQFKVDLQ
EEEECCCCHHHHHCCCCCCEEEEEEEEEEHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHH
SVLARDITFLKEYTQTFEPHVQYLYRPYRNQSNIGSTLNNDYLGFGYDSALVQQDYYSLF
HHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RDRRYSGLDRISSANQVTLGGTTRFYDIAGEERFNLSAGQIYYLSNSRIDENPANKTPTS
HHHCCCCHHHHCCCCEEEECCCEEEEECCCCCEECCCCCEEEEEECCCCCCCCCCCCCCC
SSAWALESNWKISNKWYWRGSYQFDTHTNSTSLANTSLEYNPEKNNLIQLNYRYANQEYI
CCCEEEECCCEECCEEEEEEEEEECCCCCCCCEECCCEEECCCCCCEEEEEEEECCHHHH
DQNLGKSANAYQQDIQQVGLVVGWEIANNWAVVGRYYQDLALQKPVEQYLGVQYNSCCWA
HHHCCCCCHHHHHHHHHCCEEEEEEECCCEEEEHHHHHHHHHHHHHHHHHCCEECCEEEE
ASVGVKRNVTNHQNQTRNEIVYDNSIGITLELRGLGSNDHQSGIQEMLEKGKLPYIRAFS
ECCCCCCCCCCCCCCCCCCEEEECCEEEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEE
LD
CC
>Mature Secondary Structure
MNKKHTLISLAILTALYSQQSLADLHEQCLMGVPKFSGEVVTGDVNALPVYIEADNAEIN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEEECCCCCCC
QPNDATYQGNVDLKQGNRHLLAQSVQVKQSGNQSTPLRMAYVRNGFDYKDNQINMLGKDA
CCCCCEEECCEECCCCCCEEHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEEEECCCC
EFNLDSHDGNLTNSEYEFVGRQGRGKADNITLHNNYRVMKNATFTSCLHGDNAWAVDASE
CCCCCCCCCCCCCCHHHEECCCCCCCCCEEEEECCEEEEECCCEEHEECCCCCEEECHHH
IRQYVKEEYAEMWHARFKIHGVPVFYTPYLQLPIGDRRRSGLLIPSAGTSSQDGLWYAQP
HHHHHHHHHHHHHHHEEEEECEEEEECCEEEECCCCCCCCCEEEECCCCCCCCCEEEECC
IYWNIAPNYDLTFTPKYMSRRGWQANGEFRYLTSIGEGKVAGEYLGKVRYSEYASDNRKR
EEEEECCCCCEEECHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEE
HLFYWNHNSSFLQNWRLNINYTRVSDKRYFNDFDSIYGRSTDGYANQYARIAYYQPNYNF
EEEEEECCCHHHHCEEEEEEEEEECCCHHHCCHHHHCCCCCCCCCCCEEEEEEECCCCCE
SLSAHQFQIFDDIVNIGPYRAVPQLDFNYHKYDLANGWLNFKLHSQAVRFDNDSKLMPTA
EECCCCEEHHHHHHCCCCCCCCCCCCCCCEEEECCCCEEEEEEECEEEEECCCCCCCCCC
WRFHAEPSLNSLMSNKYGSLNIETKLYATRYEQKKGSGKNAEDVQKTVNRVIPQFKVDLQ
EEEECCCCHHHHHCCCCCCEEEEEEEEEEHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHH
SVLARDITFLKEYTQTFEPHVQYLYRPYRNQSNIGSTLNNDYLGFGYDSALVQQDYYSLF
HHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RDRRYSGLDRISSANQVTLGGTTRFYDIAGEERFNLSAGQIYYLSNSRIDENPANKTPTS
HHHCCCCHHHHCCCCEEEECCCEEEEECCCCCEECCCCCEEEEEECCCCCCCCCCCCCCC
SSAWALESNWKISNKWYWRGSYQFDTHTNSTSLANTSLEYNPEKNNLIQLNYRYANQEYI
CCCEEEECCCEECCEEEEEEEEEECCCCCCCCEECCCEEECCCCCCEEEEEEEECCHHHH
DQNLGKSANAYQQDIQQVGLVVGWEIANNWAVVGRYYQDLALQKPVEQYLGVQYNSCCWA
HHHCCCCCHHHHHHHHHCCEEEEEEECCCEEEEHHHHHHHHHHHHHHHHHCCEECCEEEE
ASVGVKRNVTNHQNQTRNEIVYDNSIGITLELRGLGSNDHQSGIQEMLEKGKLPYIRAFS
ECCCCCCCCCCCCCCCCCCEEEECCEEEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEE
LD
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA