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The map label for this gene is mclA

Identifier: 16272572

GI number: 16272572

Start: 668419

End: 669006

Strand: Direct

Name: mclA

Synonym: HI0629

Alternate gene names: 16272572

Gene position: 668419-669006 (Clockwise)

Preceding gene: 16272571

Following gene: 16272573

Centisome position: 36.52

GC content: 38.44

Gene sequence:

>588_bases
ATGCAAAAAGAGCAGCTTTCAGCCTATATGGATGGAGAACAAGTCGAAACTGACTTGATAGATGCTTTATTAAGAGACGA
AGAATTACAAGCGTCTTGGCATTCATTTCATACCGTTCGTTCAGTAATGCGTAAAGAAAGTGCGGTCTTTTTAGGGGCTG
ATTTTACCGCTAAAATGGCAGATTTGATTGAGTTAGAAGACGTGAAGAAAGTTGATGTCATTGCTGTGTCTCAACCTGAA
CCAGAAGACGCACATAATTCTGTATTTATGCAAAAATTAAAAGCGTTCTTTGCACCAATGACGCAAGTTGCTGTTGCTGC
TGGTGTATGTTTAGTGGCAGTACTTGGTGTTCAATCTTTCAATAATAAAAACGATGCGTCTAATTTACCTGAGACTCCAG
TATTGCAAACCTTGCCATTTAATAATGCGGTTCAAGAGGTTAGCTATAATGCGCCAAGTAAAGATACATTAACTTCAGAT
CAGCTTGAGAAAAAAAGTCGTCGCATTGGTGCAATGTTGCAAAATTATGAATTGCAACGTCGAATGCACTCTGATGCTTT
AGATGTAAGTAGCAGTCAAGTAAGATAA

Upstream 100 bases:

>100_bases
TCCTGTTGGTACGGTTCGTTCCCGAATCTTCCGAGCAAGGGAAATGATCGAAAATAAAATTCAACCTCTAATGTAGAGCG
AATAACAATCGGAGTTTACT

Downstream 100 bases:

>100_bases
ATTGGTTTCCTTTGTTCAGATTATTGATTCACCACCTAATTGTTATTTAGTGTGGTGAATTTTTATCCTTTAGATTTCTA
TGAAAAAAATCCCATTAAAA

Product: sigma-E factor negative regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MQKEQLSAYMDGEQVETDLIDALLRDEELQASWHSFHTVRSVMRKESAVFLGADFTAKMADLIELEDVKKVDVIAVSQPE
PEDAHNSVFMQKLKAFFAPMTQVAVAAGVCLVAVLGVQSFNNKNDASNLPETPVLQTLPFNNAVQEVSYNAPSKDTLTSD
QLEKKSRRIGAMLQNYELQRRMHSDALDVSSSQVR

Sequences:

>Translated_195_residues
MQKEQLSAYMDGEQVETDLIDALLRDEELQASWHSFHTVRSVMRKESAVFLGADFTAKMADLIELEDVKKVDVIAVSQPE
PEDAHNSVFMQKLKAFFAPMTQVAVAAGVCLVAVLGVQSFNNKNDASNLPETPVLQTLPFNNAVQEVSYNAPSKDTLTSD
QLEKKSRRIGAMLQNYELQRRMHSDALDVSSSQVR
>Mature_195_residues
MQKEQLSAYMDGEQVETDLIDALLRDEELQASWHSFHTVRSVMRKESAVFLGADFTAKMADLIELEDVKKVDVIAVSQPE
PEDAHNSVFMQKLKAFFAPMTQVAVAAGVCLVAVLGVQSFNNKNDASNLPETPVLQTLPFNNAVQEVSYNAPSKDTLTSD
QLEKKSRRIGAMLQNYELQRRMHSDALDVSSSQVR

Specific function: Negative regulator of rpoE

COG id: COG3073

COG function: function code T; Negative regulator of sigma E activity

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rseA family

Homologues:

Organism=Escherichia coli, GI1788925, Length=191, Percent_Identity=40.8376963350785, Blast_Score=142, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSEA_HAEIN (P44791)

Other databases:

- EMBL:   L42023
- PIR:   G64082
- RefSeq:   NP_438789.1
- ProteinModelPortal:   P44791
- SMR:   P44791
- GeneID:   949684
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0629
- TIGR:   HI_0629
- HOGENOM:   HBG391416
- OMA:   QNQSYTN
- ProtClustDB:   CLSK870615
- BioCyc:   HINF71421:HI_0629-MONOMER
- InterPro:   IPR005573
- InterPro:   IPR005572

Pfam domain/function: PF03873 RseA_C; PF03872 RseA_N; SSF89069 Anti-sigma_E_RseA_N

EC number: NA

Molecular weight: Translated: 21733; Mature: 21733

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xc1a6c8c)-;

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKEQLSAYMDGEQVETDLIDALLRDEELQASWHSFHTVRSVMRKESAVFLGADFTAKMA
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHH
DLIELEDVKKVDVIAVSQPEPEDAHNSVFMQKLKAFFAPMTQVAVAAGVCLVAVLGVQSF
HHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NNKNDASNLPETPVLQTLPFNNAVQEVSYNAPSKDTLTSDQLEKKSRRIGAMLQNYELQR
CCCCCCCCCCCCCCEEECCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RMHSDALDVSSSQVR
HHHHHHHCCCCCCCH
>Mature Secondary Structure
MQKEQLSAYMDGEQVETDLIDALLRDEELQASWHSFHTVRSVMRKESAVFLGADFTAKMA
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHH
DLIELEDVKKVDVIAVSQPEPEDAHNSVFMQKLKAFFAPMTQVAVAAGVCLVAVLGVQSF
HHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NNKNDASNLPETPVLQTLPFNNAVQEVSYNAPSKDTLTSDQLEKKSRRIGAMLQNYELQR
CCCCCCCCCCCCCCEEECCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RMHSDALDVSSSQVR
HHHHHHHCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800