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The map label for this gene is folD

Identifier: 16272551

GI number: 16272551

Start: 640075

End: 640923

Strand: Reverse

Name: folD

Synonym: HI0609

Alternate gene names: 16272551

Gene position: 640923-640075 (Counterclockwise)

Preceding gene: 16272552

Following gene: 16272545

Centisome position: 35.02

GC content: 39.93

Gene sequence:

>849_bases
ATGGCCGCAAAAATCATCTCTGGTACTGAACTTTCAAAACAAATTAAAGCTAATTTAGCAGATAAAATAACCCATTATAT
TGAACAAGGAAAACGCGCTCCAGGACTTGCTGTAATCCTCGTTGGTGCTGATCCTGCTTCGCAAATTTATGTGGGCAATA
AACGTAAAAGTTGCGAGGAAGTCGGTATTCTCTCTAAATCTTATGACCTCCCTGAAACAACCACGCAAAATGAATTATTA
GCGATTATTGACCAACTTAACGCAGATAAAAATATTGACGGTATTTTGGTGCAACTCCCCTTGCCAAAACAAATTAATGC
GGAAGCTATTATTGAACGTATCGATCCTAAAAAAGATGTAGATGGCTTTCATCCTTATAATGTTGGGCGGTTATGCCAAC
GTATTCCAACTTTACGTGCTTGTACACCTTATGGTGTGATGAAACTATTAGAAACAACGGGTATTGATTTACACGGCAAG
CACGCAGTAATTGTAGGCGCATCCAATATTGTCGGCCGCCCAATGTCTTTAGAATTATTATTAGCAGGGGCAACCGTCAC
AGTAACACATAGATTCACTAAAAATTTGGAAAACCACGTTCGCCAAGCAGATATACTTGTCGTTGCGGTAGGTAAACCAA
ATTTAATTTCTGGCGATTGGATCAAAGAAAGTGCGGTAGTAATTGATGTTGGAATTAACCGAGTAGATGGAAAATTAGTA
GGCGATGTAGAGTTTGATAAAGCAGCCGAAAAAGCGGCTTACATTACACCAGTCCCCGGTGGCGTTGGGCCAATGACCGT
TGCAATGTTGATGAGCAATACGCTTTATGCTTATGAACACAATAAATAG

Upstream 100 bases:

>100_bases
CCACTTTAAGAAATATCCCCCTTTTTCATTGTAAAAAAATCATTGTTCGTTAAAATAGCAAACGCTTTCTTTTTCATCAC
ATAATATCAAGGATAGCTCA

Downstream 100 bases:

>100_bases
CTTTGTATAGAACGGTTATGAAAAATGGGTTAAAGCTTCATTTGCTTTAACCCATTTATTTAAATGGCTTTATTGTACTT
CTTACGTTTAAATTTATGAT

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MAAKIISGTELSKQIKANLADKITHYIEQGKRAPGLAVILVGADPASQIYVGNKRKSCEEVGILSKSYDLPETTTQNELL
AIIDQLNADKNIDGILVQLPLPKQINAEAIIERIDPKKDVDGFHPYNVGRLCQRIPTLRACTPYGVMKLLETTGIDLHGK
HAVIVGASNIVGRPMSLELLLAGATVTVTHRFTKNLENHVRQADILVVAVGKPNLISGDWIKESAVVIDVGINRVDGKLV
GDVEFDKAAEKAAYITPVPGGVGPMTVAMLMSNTLYAYEHNK

Sequences:

>Translated_282_residues
MAAKIISGTELSKQIKANLADKITHYIEQGKRAPGLAVILVGADPASQIYVGNKRKSCEEVGILSKSYDLPETTTQNELL
AIIDQLNADKNIDGILVQLPLPKQINAEAIIERIDPKKDVDGFHPYNVGRLCQRIPTLRACTPYGVMKLLETTGIDLHGK
HAVIVGASNIVGRPMSLELLLAGATVTVTHRFTKNLENHVRQADILVVAVGKPNLISGDWIKESAVVIDVGINRVDGKLV
GDVEFDKAAEKAAYITPVPGGVGPMTVAMLMSNTLYAYEHNK
>Mature_281_residues
AAKIISGTELSKQIKANLADKITHYIEQGKRAPGLAVILVGADPASQIYVGNKRKSCEEVGILSKSYDLPETTTQNELLA
IIDQLNADKNIDGILVQLPLPKQINAEAIIERIDPKKDVDGFHPYNVGRLCQRIPTLRACTPYGVMKLLETTGIDLHGKH
AVIVGASNIVGRPMSLELLLAGATVTVTHRFTKNLENHVRQADILVVAVGKPNLISGDWIKESAVVIDVGINRVDGKLVG
DVEFDKAAEKAAYITPVPGGVGPMTVAMLMSNTLYAYEHNK

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222418558, Length=291, Percent_Identity=47.0790378006873, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI94721354, Length=291, Percent_Identity=47.766323024055, Blast_Score=244, Evalue=7e-65,
Organism=Homo sapiens, GI222136639, Length=289, Percent_Identity=46.3667820069204, Blast_Score=239, Evalue=1e-63,
Organism=Homo sapiens, GI36796743, Length=213, Percent_Identity=26.7605633802817, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1786741, Length=278, Percent_Identity=67.6258992805755, Blast_Score=391, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17568735, Length=289, Percent_Identity=42.9065743944637, Blast_Score=206, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6319558, Length=283, Percent_Identity=48.0565371024735, Blast_Score=242, Evalue=4e-65,
Organism=Saccharomyces cerevisiae, GI6321643, Length=297, Percent_Identity=42.7609427609428, Blast_Score=229, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6322933, Length=316, Percent_Identity=26.5822784810127, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17136816, Length=291, Percent_Identity=48.4536082474227, Blast_Score=251, Evalue=3e-67,
Organism=Drosophila melanogaster, GI17136818, Length=291, Percent_Identity=48.4536082474227, Blast_Score=251, Evalue=3e-67,
Organism=Drosophila melanogaster, GI62472483, Length=286, Percent_Identity=44.4055944055944, Blast_Score=223, Evalue=1e-58,
Organism=Drosophila melanogaster, GI45551871, Length=286, Percent_Identity=44.4055944055944, Blast_Score=223, Evalue=1e-58,
Organism=Drosophila melanogaster, GI24645718, Length=286, Percent_Identity=44.4055944055944, Blast_Score=223, Evalue=1e-58,
Organism=Drosophila melanogaster, GI17137370, Length=286, Percent_Identity=44.4055944055944, Blast_Score=223, Evalue=1e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_HAEI8 (Q4QMI7)

Other databases:

- EMBL:   CP000057
- RefSeq:   YP_248420.1
- HSSP:   P24186
- ProteinModelPortal:   Q4QMI7
- SMR:   Q4QMI7
- STRING:   Q4QMI7
- GeneID:   3430172
- GenomeReviews:   CP000057_GR
- KEGG:   hit:NTHI0864
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   DVVYETA
- PhylomeDB:   Q4QMI7
- ProtClustDB:   PRK10792
- BioCyc:   HINF281310:NTHI0864-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30461; Mature: 30330

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKIISGTELSKQIKANLADKITHYIEQGKRAPGLAVILVGADPASQIYVGNKRKSCEE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEECCCCCCHHH
VGILSKSYDLPETTTQNELLAIIDQLNADKNIDGILVQLPLPKQINAEAIIERIDPKKDV
HCCHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCC
DGFHPYNVGRLCQRIPTLRACTPYGVMKLLETTGIDLHGKHAVIVGASNIVGRPMSLELL
CCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECHHHHCCCCEEEEE
LAGATVTVTHRFTKNLENHVRQADILVVAVGKPNLISGDWIKESAVVIDVGINRVDGKLV
EECCEEEEEHHHHHHHHHHHCEEEEEEEECCCCCEECCCCCCCCEEEEEECCCEECCEEE
GDVEFDKAAEKAAYITPVPGGVGPMTVAMLMSNTLYAYEHNK
ECCCHHHHHCCCEEEECCCCCCHHHHHHHHHHCEEEEEECCC
>Mature Secondary Structure 
AAKIISGTELSKQIKANLADKITHYIEQGKRAPGLAVILVGADPASQIYVGNKRKSCEE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEECCCCCCHHH
VGILSKSYDLPETTTQNELLAIIDQLNADKNIDGILVQLPLPKQINAEAIIERIDPKKDV
HCCHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCC
DGFHPYNVGRLCQRIPTLRACTPYGVMKLLETTGIDLHGKHAVIVGASNIVGRPMSLELL
CCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECHHHHCCCCEEEEE
LAGATVTVTHRFTKNLENHVRQADILVVAVGKPNLISGDWIKESAVVIDVGINRVDGKLV
EECCEEEEEHHHHHHHHHHHCEEEEEEEECCCCCEECCCCCCCCEEEEEECCCEECCEEE
GDVEFDKAAEKAAYITPVPGGVGPMTVAMLMSNTLYAYEHNK
ECCCHHHHHCCCEEEECCCCCCHHHHHHHHHHCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA