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The map label for this gene is cpdB

Identifier: 16272527

GI number: 16272527

Start: 603690

End: 605663

Strand: Reverse

Name: cpdB

Synonym: HI0583

Alternate gene names: 16272527

Gene position: 605663-603690 (Counterclockwise)

Preceding gene: 16272528

Following gene: 16272526

Centisome position: 33.09

GC content: 38.2

Gene sequence:

>1974_bases
ATGATGAACAGACGTCATTTTATTCAAATTAGTGCGACCAGTATTCTTGCATTAAGTGCAAACCGTTTTGCGATGGCAAA
AGGAAAGAGCGATGTTGATTTACGCATTGTGGCAACAACAGATGTTCATAGTTTCTTGACCGATTTTGACTATTATAAAG
ATGCACCAACCGATAAATTCGGTTTTACTCGTGCGGCAAGCCTTATTCGTCAAGCACGTGCTGAAGTAAAAAATAGCGTA
TTGGTAGATAATGGGGATTTAATTCAAGGTAATCCAATTGCAGACTATCAAGCCGCACAAGGCTATAAAGAGGGTAAATC
TAACCCTGCGATCGATTGTTTAAATGCGATGAATTATGAAGTGGGTACATTAGGTAACCACGAATTTAACTATGGTCTCA
ATTATCTTGCAGATGCCATCAAACAAGCTAAATTCCCAATTGTGAATTCAAACGTAGTAAAAGCGGGGACAGAAGAACCT
TATTTCACACCTTATGTCATTCAAGAAAAATCAGTTGTTGATAATCAAGGTAAAACACATAAATTAAAAATTGGTTACAT
CGGTTTTGTGCCACCGCAAATTATGGTTTGGGATAAAGCAAACCTTCAAGGCAAAGTGGAAACCCGTGATATTGTAAAAA
CAGCACAAAAATATGTACCTGAAATGAAGAAAAAAGGGGCTGATATTGTCGTTGCATTAGCTCACACAGGCCCATCTGAT
GAACCTTATCAAGAAGGTGCTGAAAACTCTGCATTCTATTTAGCGGATGTTCCACATATTGATGCGGTTATTTTTGGTCA
CTCACACCGTTTATTCCCAAATAAAGAATTTGCGAAATCACCAAATGCGGACATCGTAAATGGTACCGTAAAAGGCATAC
CAGAAAGTATGGCTGGTTACTGGGCAAATAATATCAGCGTGGTGGATTTAGGCTTAACAGAACACAAGGGTAAATGGATC
GTGACTTCTGGCAAAGCTGTACTTCGTCCAATTTATGATATAGAAACCAAAAAAGCACTTGCAAAAAATGACCCAGAAAT
TACCGCACTTTTAAAACCAGTTCACGAAGCGACACGTAAATATGTTTCTCAACCTATCGGCAAAGCCACAGACAATATGT
ATAGTTACCTTGCATTATTACAAGATGATCCAACCATTCAAATTGTAAACCAAGCACAAAAAGCGTATGTAGAGAAAGTT
GCACCAAGTATTGCAGCAATGGCTGGCTTACCTATTTTAAGTGCAGGTGCACCATTTAAAGCAGGCGGTCGTAAAAATGA
CCCAACGGGTTATACCGAAGTGAATAAAGGTAAATTAACTTTCCGTAACGCTGCAGACTTATATCTCTATCCAAATACGC
TAGTGGTTGTTAAAGCGACAGGCGAACAATTAAAAGAATGGTTAGAATGTAGTGCTGGTATGTTTAAACAAATTGACCCT
ACAAGTGATAAACCACAATCATTAATCGACTGGGAAGGTTTCCGCACTTATAACTTTGATGTGATTGATGGTGTCAATTA
TGAATACGATCTAACCAAACCAGCTCGTTACGATGGCGAATGTAAATTAATTAACCCAGAATCGCATCGTGTAGTGAATC
TCACTTATCAAGGCAAACCAGTTGATCCAAAAGCAGAATTTTTGATTGCAACGAATAACTATCGTGCTTACGGCAATAAA
TTCCCAGGTACTGGTGATAAACATATTGTTTATGCCTCGCCTGATGAAAGCCGTCAAATTTTGGCTGATTATATTAAAGC
AACCAGTGAGAAAGAAGGATCAGTTAATCCAAATGCGGATAAAAACTGGCGTTTTGTGCCTATCACAGGTAACGATAAAT
TAGATGTCCGTTTTGAAACATCGCCAAGCGAACAAGCAGCGAAATTTATCGCAGAAAAAGCACAATACCCAATGAAACAA
GTGGGTACAGATGAAATAGGTTTTGCAGTGTATCAAATTGATTTATCTAAATAA

Upstream 100 bases:

>100_bases
ATCTTAATTAAATAAAAATATTTTTACTCCTTTCTTTTAAGAATCCGCTATTTTTCTTAGAATAAAGGGAATATTTACTC
ACCCTAATAAGGAAAACTCT

Downstream 100 bases:

>100_bases
TAATTAGGAAAATAAAATGCCGTCTAAAAATTAAAATTTAGGCGGCATTTTTTCTTCCTAAAGAGCGGTCAAATTTTTGA
GTATTTTTACAAATTGCATT

Product: bifunctional 2',3'-cyclic nucleotide 2'-phosphodiesterase/3'-nucleotidase periplasmic protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 657; Mature: 657

Protein sequence:

>657_residues
MMNRRHFIQISATSILALSANRFAMAKGKSDVDLRIVATTDVHSFLTDFDYYKDAPTDKFGFTRAASLIRQARAEVKNSV
LVDNGDLIQGNPIADYQAAQGYKEGKSNPAIDCLNAMNYEVGTLGNHEFNYGLNYLADAIKQAKFPIVNSNVVKAGTEEP
YFTPYVIQEKSVVDNQGKTHKLKIGYIGFVPPQIMVWDKANLQGKVETRDIVKTAQKYVPEMKKKGADIVVALAHTGPSD
EPYQEGAENSAFYLADVPHIDAVIFGHSHRLFPNKEFAKSPNADIVNGTVKGIPESMAGYWANNISVVDLGLTEHKGKWI
VTSGKAVLRPIYDIETKKALAKNDPEITALLKPVHEATRKYVSQPIGKATDNMYSYLALLQDDPTIQIVNQAQKAYVEKV
APSIAAMAGLPILSAGAPFKAGGRKNDPTGYTEVNKGKLTFRNAADLYLYPNTLVVVKATGEQLKEWLECSAGMFKQIDP
TSDKPQSLIDWEGFRTYNFDVIDGVNYEYDLTKPARYDGECKLINPESHRVVNLTYQGKPVDPKAEFLIATNNYRAYGNK
FPGTGDKHIVYASPDESRQILADYIKATSEKEGSVNPNADKNWRFVPITGNDKLDVRFETSPSEQAAKFIAEKAQYPMKQ
VGTDEIGFAVYQIDLSK

Sequences:

>Translated_657_residues
MMNRRHFIQISATSILALSANRFAMAKGKSDVDLRIVATTDVHSFLTDFDYYKDAPTDKFGFTRAASLIRQARAEVKNSV
LVDNGDLIQGNPIADYQAAQGYKEGKSNPAIDCLNAMNYEVGTLGNHEFNYGLNYLADAIKQAKFPIVNSNVVKAGTEEP
YFTPYVIQEKSVVDNQGKTHKLKIGYIGFVPPQIMVWDKANLQGKVETRDIVKTAQKYVPEMKKKGADIVVALAHTGPSD
EPYQEGAENSAFYLADVPHIDAVIFGHSHRLFPNKEFAKSPNADIVNGTVKGIPESMAGYWANNISVVDLGLTEHKGKWI
VTSGKAVLRPIYDIETKKALAKNDPEITALLKPVHEATRKYVSQPIGKATDNMYSYLALLQDDPTIQIVNQAQKAYVEKV
APSIAAMAGLPILSAGAPFKAGGRKNDPTGYTEVNKGKLTFRNAADLYLYPNTLVVVKATGEQLKEWLECSAGMFKQIDP
TSDKPQSLIDWEGFRTYNFDVIDGVNYEYDLTKPARYDGECKLINPESHRVVNLTYQGKPVDPKAEFLIATNNYRAYGNK
FPGTGDKHIVYASPDESRQILADYIKATSEKEGSVNPNADKNWRFVPITGNDKLDVRFETSPSEQAAKFIAEKAQYPMKQ
VGTDEIGFAVYQIDLSK
>Mature_657_residues
MMNRRHFIQISATSILALSANRFAMAKGKSDVDLRIVATTDVHSFLTDFDYYKDAPTDKFGFTRAASLIRQARAEVKNSV
LVDNGDLIQGNPIADYQAAQGYKEGKSNPAIDCLNAMNYEVGTLGNHEFNYGLNYLADAIKQAKFPIVNSNVVKAGTEEP
YFTPYVIQEKSVVDNQGKTHKLKIGYIGFVPPQIMVWDKANLQGKVETRDIVKTAQKYVPEMKKKGADIVVALAHTGPSD
EPYQEGAENSAFYLADVPHIDAVIFGHSHRLFPNKEFAKSPNADIVNGTVKGIPESMAGYWANNISVVDLGLTEHKGKWI
VTSGKAVLRPIYDIETKKALAKNDPEITALLKPVHEATRKYVSQPIGKATDNMYSYLALLQDDPTIQIVNQAQKAYVEKV
APSIAAMAGLPILSAGAPFKAGGRKNDPTGYTEVNKGKLTFRNAADLYLYPNTLVVVKATGEQLKEWLECSAGMFKQIDP
TSDKPQSLIDWEGFRTYNFDVIDGVNYEYDLTKPARYDGECKLINPESHRVVNLTYQGKPVDPKAEFLIATNNYRAYGNK
FPGTGDKHIVYASPDESRQILADYIKATSEKEGSVNPNADKNWRFVPITGNDKLDVRFETSPSEQAAKFIAEKAQYPMKQ
VGTDEIGFAVYQIDLSK

Specific function: This bifunctional enzyme catalyzes two consecutive reactions during ribonucleic acid degradation. Converts a 2',3'- cyclic nucleotide to a 3'-nucleotide and then the 3'-nucleotide to the corresponding nucleoside and phosphate

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family

Homologues:

Organism=Escherichia coli, GI1790658, Length=651, Percent_Identity=62.0583717357911, Blast_Score=864, Evalue=0.0,
Organism=Escherichia coli, GI1786687, Length=605, Percent_Identity=22.9752066115702, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): CPDB_HAEIN (P44764)

Other databases:

- EMBL:   L42023
- PIR:   A64079
- RefSeq:   NP_438741.1
- ProteinModelPortal:   P44764
- GeneID:   949588
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0583
- NMPDR:   fig|71421.1.peg.554
- TIGR:   HI_0583
- HOGENOM:   HBG510269
- OMA:   ENPTTPP
- ProtClustDB:   PRK09420
- BioCyc:   HINF71421:HI_0583-MONOMER
- BRENDA:   3.1.4.16
- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR006294
- InterPro:   IPR004843
- Gene3D:   G3DSA:3.90.780.10
- PANTHER:   PTHR11575
- PRINTS:   PR01607
- TIGRFAMs:   TIGR01390

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos; SSF55816 5'-Nucleotdase_C

EC number: =3.1.3.6; =3.1.4.16

Molecular weight: Translated: 72764; Mature: 72764

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS00785 5_NUCLEOTIDASE_1; PS00786 5_NUCLEOTIDASE_2

Important sites: BINDING 450-450

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNRRHFIQISATSILALSANRFAMAKGKSDVDLRIVATTDVHSFLTDFDYYKDAPTDKF
CCCCEEEEEEEEEEEEEEECCCEEEECCCCCCEEEEEEECCHHHHHHHHHHHCCCCCCCC
GFTRAASLIRQARAEVKNSVLVDNGDLIQGNPIADYQAAQGYKEGKSNPAIDCLNAMNYE
CHHHHHHHHHHHHHHHHCCEEECCCCEECCCCCCCCHHHCCHHCCCCCCCHHHHHHCCCE
VGTLGNHEFNYGLNYLADAIKQAKFPIVNSNVVKAGTEEPYFTPYVIQEKSVVDNQGKTH
EECCCCCCCCCCHHHHHHHHHHCCCCEECCCEEECCCCCCCCCCEEEECCHHHCCCCCEE
KLKIGYIGFVPPQIMVWDKANLQGKVETRDIVKTAQKYVPEMKKKGADIVVALAHTGPSD
EEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
EPYQEGAENSAFYLADVPHIDAVIFGHSHRLFPNKEFAKSPNADIVNGTVKGIPESMAGY
CHHHCCCCCCEEEEEECCCCEEEEECCCCCCCCCHHHHCCCCCCEECCHHHCCCHHHHHH
WANNISVVDLGLTEHKGKWIVTSGKAVLRPIYDIETKKALAKNDPEITALLKPVHEATRK
HCCCEEEEEECCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
YVSQPIGKATDNMYSYLALLQDDPTIQIVNQAQKAYVEKVAPSIAAMAGLPILSAGAPFK
HHHCCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCC
AGGRKNDPTGYTEVNKGKLTFRNAADLYLYPNTLVVVKATGEQLKEWLECSAGMFKQIDP
CCCCCCCCCCCEECCCCEEEEECCCEEEECCCEEEEEEECCHHHHHHHHHCCCHHHCCCC
TSDKPQSLIDWEGFRTYNFDVIDGVNYEYDLTKPARYDGECKLINPESHRVVNLTYQGKP
CCCCCCHHCCCCCCEEECEEEECCCCCEEECCCCCCCCCEEEEECCCCCEEEEEEECCCC
VDPKAEFLIATNNYRAYGNKFPGTGDKHIVYASPDESRQILADYIKATSEKEGSVNPNAD
CCCCCCEEEEECCCEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCC
KNWRFVPITGNDKLDVRFETSPSEQAAKFIAEKAQYPMKQVGTDEIGFAVYQIDLSK
CCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHCCCHHHCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MMNRRHFIQISATSILALSANRFAMAKGKSDVDLRIVATTDVHSFLTDFDYYKDAPTDKF
CCCCEEEEEEEEEEEEEEECCCEEEECCCCCCEEEEEEECCHHHHHHHHHHHCCCCCCCC
GFTRAASLIRQARAEVKNSVLVDNGDLIQGNPIADYQAAQGYKEGKSNPAIDCLNAMNYE
CHHHHHHHHHHHHHHHHCCEEECCCCEECCCCCCCCHHHCCHHCCCCCCCHHHHHHCCCE
VGTLGNHEFNYGLNYLADAIKQAKFPIVNSNVVKAGTEEPYFTPYVIQEKSVVDNQGKTH
EECCCCCCCCCCHHHHHHHHHHCCCCEECCCEEECCCCCCCCCCEEEECCHHHCCCCCEE
KLKIGYIGFVPPQIMVWDKANLQGKVETRDIVKTAQKYVPEMKKKGADIVVALAHTGPSD
EEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
EPYQEGAENSAFYLADVPHIDAVIFGHSHRLFPNKEFAKSPNADIVNGTVKGIPESMAGY
CHHHCCCCCCEEEEEECCCCEEEEECCCCCCCCCHHHHCCCCCCEECCHHHCCCHHHHHH
WANNISVVDLGLTEHKGKWIVTSGKAVLRPIYDIETKKALAKNDPEITALLKPVHEATRK
HCCCEEEEEECCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
YVSQPIGKATDNMYSYLALLQDDPTIQIVNQAQKAYVEKVAPSIAAMAGLPILSAGAPFK
HHHCCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCC
AGGRKNDPTGYTEVNKGKLTFRNAADLYLYPNTLVVVKATGEQLKEWLECSAGMFKQIDP
CCCCCCCCCCCEECCCCEEEEECCCEEEECCCEEEEEEECCHHHHHHHHHCCCHHHCCCC
TSDKPQSLIDWEGFRTYNFDVIDGVNYEYDLTKPARYDGECKLINPESHRVVNLTYQGKP
CCCCCCHHCCCCCCEEECEEEECCCCCEEECCCCCCCCCEEEEECCCCCEEEEEEECCCC
VDPKAEFLIATNNYRAYGNKFPGTGDKHIVYASPDESRQILADYIKATSEKEGSVNPNAD
CCCCCCEEEEECCCEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCC
KNWRFVPITGNDKLDVRFETSPSEQAAKFIAEKAQYPMKQVGTDEIGFAVYQIDLSK
CCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHCCCHHHCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800