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The map label for this gene is rbsB

Identifier: 16272448

GI number: 16272448

Start: 520952

End: 521830

Strand: Direct

Name: rbsB

Synonym: HI0504

Alternate gene names: 16272448

Gene position: 520952-521830 (Clockwise)

Preceding gene: 16272447

Following gene: 16272449

Centisome position: 28.47

GC content: 39.82

Gene sequence:

>879_bases
ATGAAAAAACTAACCGCACTTACTTCTGCTGTATTATTAGGTTTGGCTGTTTCTAGCTCTGCTTCTGCGCAAGACACAAT
CGCTTTGGCAGTATCTACATTAGATAACCCATTTTTTGTAACGTTAAAAGATGGCGCACAGAAAAAAGCGGATGAATTAG
GTTATAAGTTAGTAGTGCTTGATTCACAAAATGACCCTGCTAAAGAATTAGCTAATATCGAAGATTTAACTGTGCGTGGT
GCAAAAATATTATTGATCAATCCAACTGCTTCTGAAGCTGTTGGCAACGCAGTGGCGATTGCAAACCGTAAGCATATTCC
AGTAATTACATTAGATCGTGGTGCAGCTAAAGGAAACGTAGTGAGCCATATTGCATCTGACAATATCGCAGGTGGTAAAA
TGGCAGGTGATTTTATCGCACAGAAATTAGGCGATAATGCCAAGGTGATTCAACTTGAAGGTATTGCAGGAACATCTGCG
GCACGCGAACGAGGTGAGGGTTTCAAACAAGCTATTGATGCTCATAAATTCAATGTGCTTGCCAGTCAGCCAGCAGATTT
TGATCGAACAAAAGGTTTGAATGTAACGGAAAATTTGTTGGCATCTAAAGGTGATGTTCAAGCAATTTTTGCGCAAAATG
ATGAAATGGCATTAGGTGCATTACGTGCGGTGAAAGCCGCAAATAAAAAAGTCCTTATCGTAGGTTTTGATGGTACCGAT
GATGGTGTTAAAGCGGTAAAAAGTGGCAAAATGGCGGCAACTATCGCACAACAACCCGAGCTTATTGGTTCATTAGGTGT
TGTTACCGCTGATAAAATCTTAAAGGGCGAAAAAGTTGAAGCGAAAATTCCAGTAGATTTGAAAGTAATAAGTGAATAA

Upstream 100 bases:

>100_bases
TACTATCAAATGATAGCAAAAGCGTTGGTTATTTTGGTTGCTGTTTTAGCCGATAACTATCTTGGTACAAAAAAACTGTA
ACTCACTTTAAGGAGAAACT

Downstream 100 bases:

>100_bases
TCTATCTACTAAATAAGCTCGTTATATCACATTATGGGGCAAATTATATTTGTCCCATTGTGTAGAAAGAAAAAATATAA
CAATACAGGATAAAATAATG

Product: D-ribose transporter subunit RbsB

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MKKLTALTSAVLLGLAVSSSASAQDTIALAVSTLDNPFFVTLKDGAQKKADELGYKLVVLDSQNDPAKELANIEDLTVRG
AKILLINPTASEAVGNAVAIANRKHIPVITLDRGAAKGNVVSHIASDNIAGGKMAGDFIAQKLGDNAKVIQLEGIAGTSA
ARERGEGFKQAIDAHKFNVLASQPADFDRTKGLNVTENLLASKGDVQAIFAQNDEMALGALRAVKAANKKVLIVGFDGTD
DGVKAVKSGKMAATIAQQPELIGSLGVVTADKILKGEKVEAKIPVDLKVISE

Sequences:

>Translated_292_residues
MKKLTALTSAVLLGLAVSSSASAQDTIALAVSTLDNPFFVTLKDGAQKKADELGYKLVVLDSQNDPAKELANIEDLTVRG
AKILLINPTASEAVGNAVAIANRKHIPVITLDRGAAKGNVVSHIASDNIAGGKMAGDFIAQKLGDNAKVIQLEGIAGTSA
ARERGEGFKQAIDAHKFNVLASQPADFDRTKGLNVTENLLASKGDVQAIFAQNDEMALGALRAVKAANKKVLIVGFDGTD
DGVKAVKSGKMAATIAQQPELIGSLGVVTADKILKGEKVEAKIPVDLKVISE
>Mature_292_residues
MKKLTALTSAVLLGLAVSSSASAQDTIALAVSTLDNPFFVTLKDGAQKKADELGYKLVVLDSQNDPAKELANIEDLTVRG
AKILLINPTASEAVGNAVAIANRKHIPVITLDRGAAKGNVVSHIASDNIAGGKMAGDFIAQKLGDNAKVIQLEGIAGTSA
ARERGEGFKQAIDAHKFNVLASQPADFDRTKGLNVTENLLASKGDVQAIFAQNDEMALGALRAVKAANKKVLIVGFDGTD
DGVKAVKSGKMAATIAQQPELIGSLGVVTADKILKGEKVEAKIPVDLKVISE

Specific function: Involved in the high-affinity D-ribose membrane transport system and also serves as the primary chemoreceptor for chemotaxis

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family

Homologues:

Organism=Escherichia coli, GI1790192, Length=291, Percent_Identity=73.8831615120275, Blast_Score=426, Evalue=1e-120,
Organism=Escherichia coli, GI1790526, Length=299, Percent_Identity=36.1204013377926, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1790674, Length=230, Percent_Identity=28.695652173913, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1790194, Length=230, Percent_Identity=24.7826086956522, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788473, Length=277, Percent_Identity=25.2707581227437, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787948, Length=283, Percent_Identity=25.0883392226148, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): RBSB_HAEIN (P44737)

Other databases:

- EMBL:   L42023
- PIR:   A64073
- RefSeq:   NP_438662.1
- ProteinModelPortal:   P44737
- SMR:   P44737
- GeneID:   949573
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0504
- NMPDR:   fig|71421.1.peg.475
- TIGR:   HI_0504
- HOGENOM:   HBG617058
- OMA:   NVAGGEM
- ProtClustDB:   PRK10653
- BioCyc:   HINF71421:HI_0504-MONOMER
- GO:   GO:0006935
- InterPro:   IPR001761

Pfam domain/function: PF00532 Peripla_BP_1

EC number: NA

Molecular weight: Translated: 30315; Mature: 30315

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLTALTSAVLLGLAVSSSASAQDTIALAVSTLDNPFFVTLKDGAQKKADELGYKLVVL
CCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCEEEEECCCCHHHHHHCCEEEEEE
DSQNDPAKELANIEDLTVRGAKILLINPTASEAVGNAVAIANRKHIPVITLDRGAAKGNV
ECCCCCHHHHCCCHHEEECCEEEEEECCCHHHHCCCEEEEECCCCCCEEEECCCCCCCCH
VSHIASDNIAGGKMAGDFIAQKLGDNAKVIQLEGIAGTSAARERGEGFKQAIDAHKFNVL
HHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHEEEE
ASQPADFDRTKGLNVTENLLASKGDVQAIFAQNDEMALGALRAVKAANKKVLIVGFDGTD
ECCCCCCHHCCCCCHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCC
DGVKAVKSGKMAATIAQQPELIGSLGVVTADKILKGEKVEAKIPVDLKVISE
HHHHHHHCCCEEEEHHCCCHHHHHCCCEEHHHHHCCCCEEEECCEEEEEECC
>Mature Secondary Structure
MKKLTALTSAVLLGLAVSSSASAQDTIALAVSTLDNPFFVTLKDGAQKKADELGYKLVVL
CCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCEEEEECCCCHHHHHHCCEEEEEE
DSQNDPAKELANIEDLTVRGAKILLINPTASEAVGNAVAIANRKHIPVITLDRGAAKGNV
ECCCCCHHHHCCCHHEEECCEEEEEECCCHHHHCCCEEEEECCCCCCEEEECCCCCCCCH
VSHIASDNIAGGKMAGDFIAQKLGDNAKVIQLEGIAGTSAARERGEGFKQAIDAHKFNVL
HHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHEEEE
ASQPADFDRTKGLNVTENLLASKGDVQAIFAQNDEMALGALRAVKAANKKVLIVGFDGTD
ECCCCCCHHCCCCCHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCC
DGVKAVKSGKMAATIAQQPELIGSLGVVTADKILKGEKVEAKIPVDLKVISE
HHHHHHHCCCEEEEHHCCCHHHHHCCCEEHHHHHCCCCEEEECCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023