Click here to switch to the map view.

The map label for this gene is rbsK

Identifier: 16272449

GI number: 16272449

Start: 521928

End: 522848

Strand: Direct

Name: rbsK

Synonym: HI0505

Alternate gene names: 16272449

Gene position: 521928-522848 (Clockwise)

Preceding gene: 16272448

Following gene: 16272450

Centisome position: 28.52

GC content: 42.78

Gene sequence:

>921_bases
ATGAGAAAAACCCTCACGGTTCTCGGTAGTATCAATGCTGATCACGTTATTTCTGTGCCTTACTTTACTAAACCCGGTGA
AACGCTAACAGGTCAAAATTATCAAATTGCCTATGGTGGCAAAGGTGCCAATCAAGCAGTGGCAGCCGCTCGTTTAGGGG
CAAAGGTAGCGTTCATTAGTTGCATTGGATCAGATAGCATAGGCAAAACAATGAAAAATGCGTTTGCACAAGAAGGAATT
GATACAACCCATATTAATACCGTTTCACAGGAAATGACAGGAATGGCATTTATTCAAGTCGCCAAGTCTAGTGAGAACAG
TATTGTGTTGGCTAGCGGTGCAAATTCACATTTGAGTGAGATGGTTGTTCGTCAAAGTGAGGCGCAAATTGCACAATCAG
ATTGCTTGTTAATGCAATTAGAAACCCCACTTTCTGGTGTGGAGCTGGCTGCTCAAATCGCTAAGAAAAATGGTGTCAAG
GTTGTGCTAAATCCTGCTCCAGCGCAGATTTTATCGGACGAATTATTGAGTTTGATTGATATTATTACACCAAATGAAAC
TGAAGCTGAAATCCTGACTGGGGTAGAGGTAGCCGACGAGCAAAGTGCGGTGAAAGCTGCGAGTGTTTTTCACGATAAAG
GCATTGAAACCGTAATGATTACCCTTGGCGCGAAAGGTGTGTTTGTCAGTCGAAAAGGTAAAAGCCGTATCATTAAGGGC
TTTTGTGTGCAAGCAATAGATACTACCGCAGCTGGTGATACATTTAATGGCGGTTTTGTTACGGCTTTATTGGAAGAAAA
ATCCTTTGATGAAGCCATTCGTTTTGGTCAAGCTGCCGCTGCGATTAGTGTGACGAAAAAAGGGGCTCAATCCTCTATTC
CAACGCGGCAAGAAACTTTGGAATTTCTTGAACACGCTTAA

Upstream 100 bases:

>100_bases
TAATCTATCTACTAAATAAGCTCGTTATATCACATTATGGGGCAAATTATATTTGTCCCATTGTGTAGAAAGAAAAAATA
TAACAATACAGGATAAAATA

Downstream 100 bases:

>100_bases
AGTGCGGTGAATTTTTAGGGTGTTTTTATGGCGACAATGAAAGATATTGCACGACTGGCGCAAGTTTCGACTTCCACAGT
TTCACATGTCATTAATGGAT

Product: ribokinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MRKTLTVLGSINADHVISVPYFTKPGETLTGQNYQIAYGGKGANQAVAAARLGAKVAFISCIGSDSIGKTMKNAFAQEGI
DTTHINTVSQEMTGMAFIQVAKSSENSIVLASGANSHLSEMVVRQSEAQIAQSDCLLMQLETPLSGVELAAQIAKKNGVK
VVLNPAPAQILSDELLSLIDIITPNETEAEILTGVEVADEQSAVKAASVFHDKGIETVMITLGAKGVFVSRKGKSRIIKG
FCVQAIDTTAAGDTFNGGFVTALLEEKSFDEAIRFGQAAAAISVTKKGAQSSIPTRQETLEFLEHA

Sequences:

>Translated_306_residues
MRKTLTVLGSINADHVISVPYFTKPGETLTGQNYQIAYGGKGANQAVAAARLGAKVAFISCIGSDSIGKTMKNAFAQEGI
DTTHINTVSQEMTGMAFIQVAKSSENSIVLASGANSHLSEMVVRQSEAQIAQSDCLLMQLETPLSGVELAAQIAKKNGVK
VVLNPAPAQILSDELLSLIDIITPNETEAEILTGVEVADEQSAVKAASVFHDKGIETVMITLGAKGVFVSRKGKSRIIKG
FCVQAIDTTAAGDTFNGGFVTALLEEKSFDEAIRFGQAAAAISVTKKGAQSSIPTRQETLEFLEHA
>Mature_306_residues
MRKTLTVLGSINADHVISVPYFTKPGETLTGQNYQIAYGGKGANQAVAAARLGAKVAFISCIGSDSIGKTMKNAFAQEGI
DTTHINTVSQEMTGMAFIQVAKSSENSIVLASGANSHLSEMVVRQSEAQIAQSDCLLMQLETPLSGVELAAQIAKKNGVK
VVLNPAPAQILSDELLSLIDIITPNETEAEILTGVEVADEQSAVKAASVFHDKGIETVMITLGAKGVFVSRKGKSRIIKG
FCVQAIDTTAAGDTFNGGFVTALLEEKSFDEAIRFGQAAAAISVTKKGAQSSIPTRQETLEFLEHA

Specific function: Ribose metabolism; first step. [C]

COG id: COG0524

COG function: function code G; Sugar kinases, ribokinase family

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family

Homologues:

Organism=Homo sapiens, GI11545855, Length=296, Percent_Identity=37.1621621621622, Blast_Score=160, Evalue=1e-39,
Organism=Escherichia coli, GI1790193, Length=302, Percent_Identity=55.9602649006622, Blast_Score=322, Evalue=3e-89,
Organism=Escherichia coli, GI87081971, Length=280, Percent_Identity=30.7142857142857, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI87082343, Length=274, Percent_Identity=30.2919708029197, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1788491, Length=260, Percent_Identity=25.3846153846154, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788484, Length=284, Percent_Identity=23.943661971831, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17532905, Length=311, Percent_Identity=38.5852090032154, Blast_Score=183, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6319884, Length=330, Percent_Identity=27.5757575757576, Blast_Score=115, Evalue=6e-27,
Organism=Drosophila melanogaster, GI20128807, Length=300, Percent_Identity=36.3333333333333, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24583886, Length=300, Percent_Identity=30.6666666666667, Blast_Score=130, Evalue=9e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RBSK_HAEIN (P44331)

Other databases:

- EMBL:   L42023
- PIR:   B64073
- RefSeq:   NP_438663.1
- ProteinModelPortal:   P44331
- SMR:   P44331
- GeneID:   949575
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0505
- NMPDR:   fig|71421.1.peg.476
- TIGR:   HI_0505
- HOGENOM:   HBG697973
- OMA:   VMITLGS
- ProtClustDB:   PRK11142
- BioCyc:   HINF71421:HI_0505-MONOMER
- BRENDA:   2.7.1.15
- GO:   GO:0005737
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR011877
- InterPro:   IPR002139
- PRINTS:   PR00990
- TIGRFAMs:   TIGR02152

Pfam domain/function: PF00294 PfkB

EC number: =2.7.1.15

Molecular weight: Translated: 32205; Mature: 32205

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00583 PFKB_KINASES_1; PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKTLTVLGSINADHVISVPYFTKPGETLTGQNYQIAYGGKGANQAVAAARLGAKVAFIS
CCCEEEEEECCCCCCEEECCEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCEEEEEE
CIGSDSIGKTMKNAFAQEGIDTTHINTVSQEMTGMAFIQVAKSSENSIVLASGANSHLSE
EECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEEEEECCCCCCEEEEECCCHHHHH
MVVRQSEAQIAQSDCLLMQLETPLSGVELAAQIAKKNGVKVVLNPAPAQILSDELLSLID
HHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHH
IITPNETEAEILTGVEVADEQSAVKAASVFHDKGIETVMITLGAKGVFVSRKGKSRIIKG
HCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCEEEEECCCCHHHHHH
FCVQAIDTTAAGDTFNGGFVTALLEEKSFDEAIRFGQAAAAISVTKKGAQSSIPTRQETL
HHHHHHHCCCCCCCCCCCEEEEEEHHCCHHHHHHHCCHHHEEEEHHCCCCCCCCCHHHHH
EFLEHA
HHHHCC
>Mature Secondary Structure
MRKTLTVLGSINADHVISVPYFTKPGETLTGQNYQIAYGGKGANQAVAAARLGAKVAFIS
CCCEEEEEECCCCCCEEECCEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCEEEEEE
CIGSDSIGKTMKNAFAQEGIDTTHINTVSQEMTGMAFIQVAKSSENSIVLASGANSHLSE
EECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEEEEECCCCCCEEEEECCCHHHHH
MVVRQSEAQIAQSDCLLMQLETPLSGVELAAQIAKKNGVKVVLNPAPAQILSDELLSLID
HHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHH
IITPNETEAEILTGVEVADEQSAVKAASVFHDKGIETVMITLGAKGVFVSRKGKSRIIKG
HCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCEEEEECCCCHHHHHH
FCVQAIDTTAAGDTFNGGFVTALLEEKSFDEAIRFGQAAAAISVTKKGAQSSIPTRQETL
HHHHHHHCCCCCCCCCCCEEEEEEHHCCHHHHHHHCCHHHEEEEHHCCCCCCCCCHHHHH
EFLEHA
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800