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The map label for this gene is hisF

Identifier: 16272422

GI number: 16272422

Start: 496722

End: 497498

Strand: Direct

Name: hisF

Synonym: HI0474

Alternate gene names: 16272422

Gene position: 496722-497498 (Clockwise)

Preceding gene: 16272421

Following gene: 16272423

Centisome position: 27.14

GC content: 42.47

Gene sequence:

>777_bases
ATGTTGGCAAAACGGATAATTCCTTGTTTGGATGTACGTGATGGACAGGTGGTAAAAGGGGTACAATTCCGTAACCACGA
AATTATTGGCGATATTGTGCCACTTGCTCAACGCTATGCGCAAGAAGGAGCTGATGAATTGGTATTTTATGATATTACGG
CTTCATCAGACGGTCGTACCGTGGATAAAAGTTGGGTAGAACGAATCGCTCAGGTAATTGATATTCCGTTTTGCGTAGCA
GGTGGGATTAAAACCATTGAAGATGCGGAAAAATTGTTTGCATTTGGGGCGGATAAAATTTCGATTAATTCCCCTGCTCT
CGCTGATCCAACGCTCATTTCTCGCTTAGCAGATCGTTTTGGCGTACAAGCGATTGTAGTAGGTATTGATAGTTGGTTTG
AACAGGAAACAGGTAAGTATTGGGTAAACCAATATACAGGCGATGAAACACGTACCCGACAAACTCATTGGCAGTTGTTA
GATTGGGTAAAAGAAGTACAACAATGCGGTGCAGGAGAAATCGTGCTAAATATGATGAATCAAGATGGTTTACGAAACGG
GTACGATTTAGCCCAGCTCAAGCTGGTGCGAGGAGTATGTAGAGTACCTCTAATTGCATCTGGCGGTGCGGGTAAAATGG
TACATTTTCGTGATGCTTTTATTGAAGCAAAAGTAGATGGGGCATTAGCTGCCAGCGTATTCCATAAACAGATTATTGAA
ATTGGCGAGTTAAAATCCTACCTTGTGCAATCAGCCATCGAGATTCGGAGTGAATAA

Upstream 100 bases:

>100_bases
CACTGGCTGATATTGAGGCTCTAAAAGGCACAGGTGTGTCAGGTGTAATTGTAGGTCGAGCTTTATTAGAAGGAAAATTT
ACGCTTTCGGAGGCAATCAA

Downstream 100 bases:

>100_bases
TGAATATTACTAAAATAGACTGGCAAAAAGTTAATGGTCTTCTTCCTGTTATTATTCAAAATATAAGTACTCGTGAGGTA
CTAATGCTTGGCTATATGAA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAQRYAQEGADELVFYDITASSDGRTVDKSWVERIAQVIDIPFCVA
GGIKTIEDAEKLFAFGADKISINSPALADPTLISRLADRFGVQAIVVGIDSWFEQETGKYWVNQYTGDETRTRQTHWQLL
DWVKEVQQCGAGEIVLNMMNQDGLRNGYDLAQLKLVRGVCRVPLIASGGAGKMVHFRDAFIEAKVDGALAASVFHKQIIE
IGELKSYLVQSAIEIRSE

Sequences:

>Translated_258_residues
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAQRYAQEGADELVFYDITASSDGRTVDKSWVERIAQVIDIPFCVA
GGIKTIEDAEKLFAFGADKISINSPALADPTLISRLADRFGVQAIVVGIDSWFEQETGKYWVNQYTGDETRTRQTHWQLL
DWVKEVQQCGAGEIVLNMMNQDGLRNGYDLAQLKLVRGVCRVPLIASGGAGKMVHFRDAFIEAKVDGALAASVFHKQIIE
IGELKSYLVQSAIEIRSE
>Mature_258_residues
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAQRYAQEGADELVFYDITASSDGRTVDKSWVERIAQVIDIPFCVA
GGIKTIEDAEKLFAFGADKISINSPALADPTLISRLADRFGVQAIVVGIDSWFEQETGKYWVNQYTGDETRTRQTHWQLL
DWVKEVQQCGAGEIVLNMMNQDGLRNGYDLAQLKLVRGVCRVPLIASGGAGKMVHFRDAFIEAKVDGALAASVFHKQIIE
IGELKSYLVQSAIEIRSE

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=256, Percent_Identity=82.03125, Blast_Score=437, Evalue=1e-124,
Organism=Escherichia coli, GI87082028, Length=215, Percent_Identity=26.046511627907, Blast_Score=65, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6319725, Length=317, Percent_Identity=32.4921135646688, Blast_Score=135, Evalue=8e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_HAEI8 (Q4QN69)

Other databases:

- EMBL:   CP000057
- RefSeq:   YP_248188.1
- ProteinModelPortal:   Q4QN69
- SMR:   Q4QN69
- STRING:   Q4QN69
- GeneID:   3429915
- GenomeReviews:   CP000057_GR
- KEGG:   hit:NTHI0605
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   ASVFHKG
- PhylomeDB:   Q4QN69
- ProtClustDB:   PRK02083
- BioCyc:   HINF281310:NTHI0605-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 28626; Mature: 28626

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAQRYAQEGADELVFYDITASSDGRT
CCHHHCCCCCCCCCCCEEECEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCC
VDKSWVERIAQVIDIPFCVAGGIKTIEDAEKLFAFGADKISINSPALADPTLISRLADRF
CCHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHH
GVQAIVVGIDSWFEQETGKYWVNQYTGDETRTRQTHWQLLDWVKEVQQCGAGEIVLNMMN
CCEEEEEEHHHHHHHCCCCEEHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QDGLRNGYDLAQLKLVRGVCRVPLIASGGAGKMVHFRDAFIEAKVDGALAASVFHKQIIE
CCCCCCCCHHHHHHHHHHHHHCCEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHH
IGELKSYLVQSAIEIRSE
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAQRYAQEGADELVFYDITASSDGRT
CCHHHCCCCCCCCCCCEEECEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCC
VDKSWVERIAQVIDIPFCVAGGIKTIEDAEKLFAFGADKISINSPALADPTLISRLADRF
CCHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHH
GVQAIVVGIDSWFEQETGKYWVNQYTGDETRTRQTHWQLLDWVKEVQQCGAGEIVLNMMN
CCEEEEEEHHHHHHHCCCCEEHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QDGLRNGYDLAQLKLVRGVCRVPLIASGGAGKMVHFRDAFIEAKVDGALAASVFHKQIIE
CCCCCCCCHHHHHHHHHHHHHCCEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHH
IGELKSYLVQSAIEIRSE
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA