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The map label for this gene is hisIE

Identifier: 16272423

GI number: 16272423

Start: 497498

End: 498163

Strand: Direct

Name: hisIE

Synonym: HI0475

Alternate gene names: 16272423

Gene position: 497498-498163 (Clockwise)

Preceding gene: 16272422

Following gene: 16272424

Centisome position: 27.18

GC content: 37.09

Gene sequence:

>666_bases
ATGAATATTACTAAAATAGACTGGCAAAAAGTTAATGGTCTTCTTCCTGTTATTATTCAAAATATAAGTACTCGTGAGGT
ACTAATGCTTGGCTATATGAATGAAGAAGCCCTTACAAAAACCATCAAAGAACGTAAAGTAACTTTCTTCTCTCGTACCA
AACAACGATTATGGACAAAAGGAGAAATCTCGGGCAATTTTTTAAATGTGGAAGAAATGAGCTTAGATTGCGATAACGAC
ACTTTGCTCATTTTAGTTGATCCCATTGGGGCAACTTGCCACACAGGTGAATACAGCTGCTTCCATCAATTTACCTCTCC
ACAATCAGAGAACAAGAAGCAACAATTTGCAAATTGGGCGTGGTTTATAAAATTAGAACAACATTTAAAAGAAAAGAAAA
ATGCAGATCCTTCTAATTCTTATACTGCAACATTACACGCAAAAGGTACCAAAAAAATCGCCCAAAAAGTGGGGGAAGAA
GGTGTGGAAACTGCCTTGGCTGCGGTCGCACAAGATAAAGCGGAAGTCATTTCTGAAGCTACAGATTTGGTCTATCACTT
AACCGTATTATTACACAATCAAGATCTTCAGTGGTACGAAATTATCGCAAAACTACAAGAACGCCATCAAGGGATTGGAT
TACATCCAGAAGGAGGAAATAAATAA

Upstream 100 bases:

>100_bases
ATGGGGCATTAGCTGCCAGCGTATTCCATAAACAGATTATTGAAATTGGCGAGTTAAAATCCTACCTTGTGCAATCAGCC
ATCGAGATTCGGAGTGAATA

Downstream 100 bases:

>100_bases
GAAAATTGTCAGGCAATATTAAGTGAGCAATTAGAAGATTTGTTTGGGCAATTCAATATCATCGTAAAATACAGTGAAAC
TATGGTAATTAAATGTATTG

Product: bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein

Products: NA

Alternate protein names: Phosphoribosyl-AMP cyclohydrolase; PRA-CH; Phosphoribosyl-ATP pyrophosphatase; PRA-PH

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MNITKIDWQKVNGLLPVIIQNISTREVLMLGYMNEEALTKTIKERKVTFFSRTKQRLWTKGEISGNFLNVEEMSLDCDND
TLLILVDPIGATCHTGEYSCFHQFTSPQSENKKQQFANWAWFIKLEQHLKEKKNADPSNSYTATLHAKGTKKIAQKVGEE
GVETALAAVAQDKAEVISEATDLVYHLTVLLHNQDLQWYEIIAKLQERHQGIGLHPEGGNK

Sequences:

>Translated_221_residues
MNITKIDWQKVNGLLPVIIQNISTREVLMLGYMNEEALTKTIKERKVTFFSRTKQRLWTKGEISGNFLNVEEMSLDCDND
TLLILVDPIGATCHTGEYSCFHQFTSPQSENKKQQFANWAWFIKLEQHLKEKKNADPSNSYTATLHAKGTKKIAQKVGEE
GVETALAAVAQDKAEVISEATDLVYHLTVLLHNQDLQWYEIIAKLQERHQGIGLHPEGGNK
>Mature_221_residues
MNITKIDWQKVNGLLPVIIQNISTREVLMLGYMNEEALTKTIKERKVTFFSRTKQRLWTKGEISGNFLNVEEMSLDCDND
TLLILVDPIGATCHTGEYSCFHQFTSPQSENKKQQFANWAWFIKLEQHLKEKKNADPSNSYTATLHAKGTKKIAQKVGEE
GVETALAAVAQDKAEVISEATDLVYHLTVLLHNQDLQWYEIIAKLQERHQGIGLHPEGGNK

Specific function: Histidine biosynthesis; second step. Histidine biosynthesis; third step. [C]

COG id: COG0139

COG function: function code E; Phosphoribosyl-AMP cyclohydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PRA-PH family

Homologues:

Organism=Escherichia coli, GI2367128, Length=206, Percent_Identity=59.2233009708738, Blast_Score=247, Evalue=4e-67,
Organism=Saccharomyces cerevisiae, GI6319819, Length=184, Percent_Identity=28.804347826087, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS2_HAEIN (P44434)

Other databases:

- EMBL:   L42023
- PIR:   A64071
- RefSeq:   NP_438636.1
- ProteinModelPortal:   P44434
- GeneID:   949566
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0475
- TIGR:   HI_0475
- HOGENOM:   HBG294308
- OMA:   VMACKDD
- ProtClustDB:   PRK02759
- BioCyc:   HINF71421:HI_0475-MONOMER
- BRENDA:   3.5.4.19
- BRENDA:   3.6.1.31
- GO:   GO:0005737
- HAMAP:   MF_01019
- InterPro:   IPR023019
- InterPro:   IPR008179
- InterPro:   IPR021130
- InterPro:   IPR002496
- ProDom:   PD002610
- TIGRFAMs:   TIGR03188

Pfam domain/function: PF01502 PRA-CH; PF01503 PRA-PH

EC number: =3.5.4.19; =3.6.1.31

Molecular weight: Translated: 25097; Mature: 25097

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNITKIDWQKVNGLLPVIIQNISTREVLMLGYMNEEALTKTIKERKVTFFSRTKQRLWTK
CCCCCCCHHHHCCHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCC
GEISGNFLNVEEMSLDCDNDTLLILVDPIGATCHTGEYSCFHQFTSPQSENKKQQFANWA
CCCCCCEEEHHHCEECCCCCEEEEEECCCCCEEECCCHHHHHHHCCCCCCCHHHHHHHHH
WFIKLEQHLKEKKNADPSNSYTATLHAKGTKKIAQKVGEEGVETALAAVAQDKAEVISEA
HEEHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
TDLVYHLTVLLHNQDLQWYEIIAKLQERHQGIGLHPEGGNK
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNITKIDWQKVNGLLPVIIQNISTREVLMLGYMNEEALTKTIKERKVTFFSRTKQRLWTK
CCCCCCCHHHHCCHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCC
GEISGNFLNVEEMSLDCDNDTLLILVDPIGATCHTGEYSCFHQFTSPQSENKKQQFANWA
CCCCCCEEEHHHCEECCCCCEEEEEECCCCCEEECCCHHHHHHHCCCCCCCHHHHHHHHH
WFIKLEQHLKEKKNADPSNSYTATLHAKGTKKIAQKVGEEGVETALAAVAQDKAEVISEA
HEEHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
TDLVYHLTVLLHNQDLQWYEIIAKLQERHQGIGLHPEGGNK
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800