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The map label for this gene is holB

Identifier: 16272403

GI number: 16272403

Start: 476358

End: 477341

Strand: Reverse

Name: holB

Synonym: HI0455

Alternate gene names: 16272403

Gene position: 477341-476358 (Counterclockwise)

Preceding gene: 30995377

Following gene: 16272402

Centisome position: 26.08

GC content: 40.14

Gene sequence:

>984_bases
ATGACCGCACTTTACCCTTGGCTAATGCCAATTTATCATCAAATTGCTCAAACCTTTGACGAAGGGTTGGGGCATCATGC
TGTGCTGATTAAAGCTGATTCTGGTTTAGGCGTAGAGAGTTTATTTAATGCACTTGCACAGAAAATAATGTGTGTAGCTC
AAGGCGATAAACCTTGTGGTCAATGCCATTCTTGTCATTTAATGCAAGCCCATAGCCATCCAGATTATCACGAATTAAGC
CCCATTAACGGTAAGGATATTGGCGTTGATCAAGTACGCGACATTAATGAAATGGTTGCGCAGCACGCACAACAAAACGG
CAATAAAGTGGTGTATGTGCAAGGGGCGGAACGTTTAACGGAAGCGGCTGCTAATGCATTATTGAAAACATTGGAAGAGC
CTCGTCCAAATACTTATTTTTTACTTCAAGCGGATAGTTCGGCAAGTTTGTTAGCAACTATTTACAGTCGATGCCAAGTG
TGGAATCTTTCCGTGCCTAATGAAGAAATTGCTTTTGAATGGTTAAAATCAAAAAGTGCGGTAGAAAATCAGGAAATTTT
GACCGCACTTGCGATGAATCTTGGGCGTCCGCTTTTAGCATTAGAAACGTTACAAGAAGGATTTATTGAACAGCGTAAAA
ACTTCTTACGTCAATTTTGGGTGTTCTATCGCCGACGTTCGCCATTGGAATTGCTTCCGTTGTTTGATAAAGAACGCTAT
GTTCAGCAAGTGGATTGGATTTTGGCTTTTCTTTCTGATTGTTTAAAACATAAACTTGAAATTGATAGTCATCGACAAGT
GGCTGATCTTGGCCGTGGTATCGAACAATTCAGCGACGAGCAAACTGCCCTTGGTTTATTACAAGCCATTAAAATTATGC
AAAAAGTGCGGTCAGATTTGCTTACAATTAATGGTGTGAATGTTGAATTAATGCTATTGGATGGCTTGACACGATTAGTC
ACAGAAGTATTTGAAACGCAATAA

Upstream 100 bases:

>100_bases
ATAATCCCAAAGCAGTGGTGATTAATGCAGAGCAGAGTATTGAACTTGTTCAAGCTGATATTGAAAGTGCGGTAAAAAAT
TGGTGGAAATCAAACGAAAA

Downstream 100 bases:

>100_bases
ATTAAAGATATATCGTCTATGGCGTTCAAGCCTTAGGCATAAGGAAAAATAATTATGTTCATTGTTGACTCCCACTGCCA
TTTAGATGCGTTGGATTATG

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MTALYPWLMPIYHQIAQTFDEGLGHHAVLIKADSGLGVESLFNALAQKIMCVAQGDKPCGQCHSCHLMQAHSHPDYHELS
PINGKDIGVDQVRDINEMVAQHAQQNGNKVVYVQGAERLTEAAANALLKTLEEPRPNTYFLLQADSSASLLATIYSRCQV
WNLSVPNEEIAFEWLKSKSAVENQEILTALAMNLGRPLLALETLQEGFIEQRKNFLRQFWVFYRRRSPLELLPLFDKERY
VQQVDWILAFLSDCLKHKLEIDSHRQVADLGRGIEQFSDEQTALGLLQAIKIMQKVRSDLLTINGVNVELMLLDGLTRLV
TEVFETQ

Sequences:

>Translated_327_residues
MTALYPWLMPIYHQIAQTFDEGLGHHAVLIKADSGLGVESLFNALAQKIMCVAQGDKPCGQCHSCHLMQAHSHPDYHELS
PINGKDIGVDQVRDINEMVAQHAQQNGNKVVYVQGAERLTEAAANALLKTLEEPRPNTYFLLQADSSASLLATIYSRCQV
WNLSVPNEEIAFEWLKSKSAVENQEILTALAMNLGRPLLALETLQEGFIEQRKNFLRQFWVFYRRRSPLELLPLFDKERY
VQQVDWILAFLSDCLKHKLEIDSHRQVADLGRGIEQFSDEQTALGLLQAIKIMQKVRSDLLTINGVNVELMLLDGLTRLV
TEVFETQ
>Mature_326_residues
TALYPWLMPIYHQIAQTFDEGLGHHAVLIKADSGLGVESLFNALAQKIMCVAQGDKPCGQCHSCHLMQAHSHPDYHELSP
INGKDIGVDQVRDINEMVAQHAQQNGNKVVYVQGAERLTEAAANALLKTLEEPRPNTYFLLQADSSASLLATIYSRCQVW
NLSVPNEEIAFEWLKSKSAVENQEILTALAMNLGRPLLALETLQEGFIEQRKNFLRQFWVFYRRRSPLELLPLFDKERYV
QQVDWILAFLSDCLKHKLEIDSHRQVADLGRGIEQFSDEQTALGLLQAIKIMQKVRSDLLTINGVNVELMLLDGLTRLVT
EVFETQ

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=317, Percent_Identity=35.3312302839117, Blast_Score=168, Evalue=5e-43,
Organism=Escherichia coli, GI1786676, Length=151, Percent_Identity=32.4503311258278, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HOLB_HAEIN (P43748)

Other databases:

- EMBL:   L42023
- RefSeq:   NP_438616.1
- ProteinModelPortal:   P43748
- SMR:   P43748
- GeneID:   949546
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0455
- NMPDR:   fig|71421.1.peg.429
- TIGR:   HI_0455
- HOGENOM:   HBG729459
- OMA:   CHSCHLM
- ProtClustDB:   PRK06871
- BioCyc:   HINF71421:HI_0455-MONOMER
- BRENDA:   2.7.7.7
- InterPro:   IPR008921
- InterPro:   IPR004622
- InterPro:   IPR015199
- TIGRFAMs:   TIGR00678

Pfam domain/function: PF09115 DNApol3-delta_C; SSF48019 Pol_clamp_load_C

EC number: =2.7.7.7

Molecular weight: Translated: 37039; Mature: 36908

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALYPWLMPIYHQIAQTFDEGLGHHAVLIKADSGLGVESLFNALAQKIMCVAQGDKPCG
CCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCC
QCHSCHLMQAHSHPDYHELSPINGKDIGVDQVRDINEMVAQHAQQNGNKVVYVQGAERLT
CCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHH
EAAANALLKTLEEPRPNTYFLLQADSSASLLATIYSRCQVWNLSVPNEEIAFEWLKSKSA
HHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHH
VENQEILTALAMNLGRPLLALETLQEGFIEQRKNFLRQFWVFYRRRSPLELLPLFDKERY
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHH
VQQVDWILAFLSDCLKHKLEIDSHRQVADLGRGIEQFSDEQTALGLLQAIKIMQKVRSDL
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LTINGVNVELMLLDGLTRLVTEVFETQ
EEECCCCEEEEHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TALYPWLMPIYHQIAQTFDEGLGHHAVLIKADSGLGVESLFNALAQKIMCVAQGDKPCG
CCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCC
QCHSCHLMQAHSHPDYHELSPINGKDIGVDQVRDINEMVAQHAQQNGNKVVYVQGAERLT
CCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHH
EAAANALLKTLEEPRPNTYFLLQADSSASLLATIYSRCQVWNLSVPNEEIAFEWLKSKSA
HHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHH
VENQEILTALAMNLGRPLLALETLQEGFIEQRKNFLRQFWVFYRRRSPLELLPLFDKERY
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHH
VQQVDWILAFLSDCLKHKLEIDSHRQVADLGRGIEQFSDEQTALGLLQAIKIMQKVRSDL
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LTINGVNVELMLLDGLTRLVTEVFETQ
EEECCCCEEEEHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800