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The map label for this gene is tmk [H]

Identifier: 30995377

GI number: 30995377

Start: 477338

End: 477997

Strand: Reverse

Name: tmk [H]

Synonym: HI0456

Alternate gene names: 30995377

Gene position: 477997-477338 (Counterclockwise)

Preceding gene: 16272405

Following gene: 16272403

Centisome position: 26.12

GC content: 42.12

Gene sequence:

>660_bases
ATGCCAAATAAAAGTGCGGAAGAAAATATGAAAGGAAAGTTTATTGTCATTGAGGGCTTAGAAGGTGCGGGGAAAAGCTC
CGCTCATCAGTCTGTAGTGCGAGTTTTGCATGAACTTGGTATTCAAGATGTTGTGTTTACGCGCGAGCCTGGTGGAACGC
CACTGGCTGAGAAATTACGTCATCTCATCAAACATGAAACCGAAGAACCCGTGACAGATAAAGCAGAGTTATTAATGCTT
TATGCGGCTCGTATTCAGTTGGTGGAAAATGTGATTAAACCTGCTTTAATGCAAGGGAAATGGGTAGTGGGCGATCGTCA
CGATATGTCATCTCAGGCGTATCAGGGTGGTGGACGTCAATTAGACCCGCATTTTATGCTCACCTTGAAAGAAACCGTAT
TAGGTAATTTTGAGCCAGATCTCACAATTTATTTGGATATAGATCCGAGCGTCGGTTTAGCGCGAGCTCGTGGACGTGGC
GAGTTAGATCGTATTGAGCAAATGGATTTAGATTTTTTCCATCGTACTCGAGCACGCTATTTAGAATTAGTAAAAGATAA
TCCCAAAGCAGTGGTGATTAATGCAGAGCAGAGTATTGAACTTGTTCAAGCTGATATTGAAAGTGCGGTAAAAAATTGGT
GGAAATCAAACGAAAAATGA

Upstream 100 bases:

>100_bases
TTTGTAGCAGATGGTTCTGGTGGGCATAAATTTACCCGAAATTTGAATGAACATAACAAAGCGGTGCAAGAATATTTACG
TTGGTATCGCAGTCAGAAAA

Downstream 100 bases:

>100_bases
CCGCACTTTACCCTTGGCTAATGCCAATTTATCATCAAATTGCTCAAACCTTTGACGAAGGGTTGGGGCATCATGCTGTG
CTGATTAAAGCTGATTCTGG

Product: thymidylate kinase

Products: NA

Alternate protein names: dTMP kinase [H]

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MPNKSAEENMKGKFIVIEGLEGAGKSSAHQSVVRVLHELGIQDVVFTREPGGTPLAEKLRHLIKHETEEPVTDKAELLML
YAARIQLVENVIKPALMQGKWVVGDRHDMSSQAYQGGGRQLDPHFMLTLKETVLGNFEPDLTIYLDIDPSVGLARARGRG
ELDRIEQMDLDFFHRTRARYLELVKDNPKAVVINAEQSIELVQADIESAVKNWWKSNEK

Sequences:

>Translated_219_residues
MPNKSAEENMKGKFIVIEGLEGAGKSSAHQSVVRVLHELGIQDVVFTREPGGTPLAEKLRHLIKHETEEPVTDKAELLML
YAARIQLVENVIKPALMQGKWVVGDRHDMSSQAYQGGGRQLDPHFMLTLKETVLGNFEPDLTIYLDIDPSVGLARARGRG
ELDRIEQMDLDFFHRTRARYLELVKDNPKAVVINAEQSIELVQADIESAVKNWWKSNEK
>Mature_218_residues
PNKSAEENMKGKFIVIEGLEGAGKSSAHQSVVRVLHELGIQDVVFTREPGGTPLAEKLRHLIKHETEEPVTDKAELLMLY
AARIQLVENVIKPALMQGKWVVGDRHDMSSQAYQGGGRQLDPHFMLTLKETVLGNFEPDLTIYLDIDPSVGLARARGRGE
LDRIEQMDLDFFHRTRARYLELVKDNPKAVVINAEQSIELVQADIESAVKNWWKSNEK

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis [H]

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family [H]

Homologues:

Organism=Escherichia coli, GI1787340, Length=209, Percent_Identity=62.200956937799, Blast_Score=268, Evalue=2e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018095
- InterPro:   IPR018094 [H]

Pfam domain/function: PF02223 Thymidylate_kin [H]

EC number: =2.7.4.9 [H]

Molecular weight: Translated: 24695; Mature: 24563

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNKSAEENMKGKFIVIEGLEGAGKSSAHQSVVRVLHELGIQDVVFTREPGGTPLAEKLR
CCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHH
HLIKHETEEPVTDKAELLMLYAARIQLVENVIKPALMQGKWVVGDRHDMSSQAYQGGGRQ
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHCCCCCC
LDPHFMLTLKETVLGNFEPDLTIYLDIDPSVGLARARGRGELDRIEQMDLDFFHRTRARY
CCCHHEEEEHHHHCCCCCCCEEEEEEECCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LELVKDNPKAVVINAEQSIELVQADIESAVKNWWKSNEK
HHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PNKSAEENMKGKFIVIEGLEGAGKSSAHQSVVRVLHELGIQDVVFTREPGGTPLAEKLR
CCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHH
HLIKHETEEPVTDKAELLMLYAARIQLVENVIKPALMQGKWVVGDRHDMSSQAYQGGGRQ
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHCCCCCC
LDPHFMLTLKETVLGNFEPDLTIYLDIDPSVGLARARGRGELDRIEQMDLDFFHRTRARY
CCCHHEEEEHHHHCCCCCCCEEEEEEECCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LELVKDNPKAVVINAEQSIELVQADIESAVKNWWKSNEK
HHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA