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The map label for this gene is fruK

Identifier: 16272395

GI number: 16272395

Start: 469937

End: 470878

Strand: Reverse

Name: fruK

Synonym: HI0447

Alternate gene names: 16272395

Gene position: 470878-469937 (Counterclockwise)

Preceding gene: 16272396

Following gene: 16272394

Centisome position: 25.73

GC content: 40.87

Gene sequence:

>942_bases
ATGGCAAGCGTCGTAACCATTACCTTAAATGCCGCCTATGATTTAGTTGGGCGTTTAAATCGTATTCAACTTGGCGAAGT
AAATACGGTAGAAACATTGGGCTTATTCCCTGCTGGAAAAGGCATTAATGTCGCAAAAGTACTAAAAGATTTAGGCGTTA
ATGTTGCAGTAGGCGGTTTCTTGGGCAAGGATAATTCAGCTGATTTTGAGCAAATGTTTAATCAGCACGGTTTGGAAGAT
AAATTCCACCGTGTTGATGGCAAAACGCGTATCAATGTAAAAATTACCGAGACTGAAGCGGATGTGACAGATTTAAATTT
CTTGGGGTATCAAATAAGCCCACAAGTTTGGCAACAATTTGTGACGGATTCTTTGGCTTATTGTTTAAATTATGACATTG
TCGCAGTTTGTGGCAGTTTACCTCGAGGTGTGTCTCCTGAATTGTTTGCTGATTGGTTGAATCAGCTTCATCAAGCTGGC
GTTAAAGTGGTGCTTGATAGTAGCAATGCTGCGCTTACCGCTGGATTAAAAGCGAAACCTTGGCTAGTAAAACCCAATCA
TCGAGAACTTGAGGCTTGGGTTGGTCATCCATTAAATAGTCTTGAAGAAATTATTGCCGCTGCACAGCAACTCAAAGCGG
AAGGCATTGAAAACGTGATTATTTCAATGGGCGCAAAAGGTTCTTTATGGATTAATAATGAAGGCGTGCTCAAAGCCGAA
CCAGCACAATGTGAAAATGTCGTGAGTACCGTTGGAGCAGGGGATTCAATGGTAGCAGGCTTAATCTATGGTTTTGAAAA
AGGTTTATCGAAAACAGAAACTTTAGCTTTTGCTACGGCTGTGTCCGCTTTTGCTGTATCTCAGAGTAACGTCGGTGTGA
GTGATTTGAGTTTACTTGACCCTATTTTAGAAAAAGTACAAATAACGATGATTGAAGGATAG

Upstream 100 bases:

>100_bases
GTCAAAGGGACTCGTTTACGTTTTGTTGCGACGGGCGAAGAAGCGCAACAAGCTATTGACGGAATCGGTGCTGTGATCGA
ATCGGGTTTAGGGGAATAGT

Downstream 100 bases:

>100_bases
TATGAAGTTATTTTTAACCCAATCAGCTAATGTAGGCGATGTGAAAGCCTATTTATTACATGAAGTTTTTCGAGCAGCTG
CACAAAAAGCAAATGTCTCT

Product: 1-phosphofructokinase

Products: NA

Alternate protein names: Fructose 1-phosphate kinase

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MASVVTITLNAAYDLVGRLNRIQLGEVNTVETLGLFPAGKGINVAKVLKDLGVNVAVGGFLGKDNSADFEQMFNQHGLED
KFHRVDGKTRINVKITETEADVTDLNFLGYQISPQVWQQFVTDSLAYCLNYDIVAVCGSLPRGVSPELFADWLNQLHQAG
VKVVLDSSNAALTAGLKAKPWLVKPNHRELEAWVGHPLNSLEEIIAAAQQLKAEGIENVIISMGAKGSLWINNEGVLKAE
PAQCENVVSTVGAGDSMVAGLIYGFEKGLSKTETLAFATAVSAFAVSQSNVGVSDLSLLDPILEKVQITMIEG

Sequences:

>Translated_313_residues
MASVVTITLNAAYDLVGRLNRIQLGEVNTVETLGLFPAGKGINVAKVLKDLGVNVAVGGFLGKDNSADFEQMFNQHGLED
KFHRVDGKTRINVKITETEADVTDLNFLGYQISPQVWQQFVTDSLAYCLNYDIVAVCGSLPRGVSPELFADWLNQLHQAG
VKVVLDSSNAALTAGLKAKPWLVKPNHRELEAWVGHPLNSLEEIIAAAQQLKAEGIENVIISMGAKGSLWINNEGVLKAE
PAQCENVVSTVGAGDSMVAGLIYGFEKGLSKTETLAFATAVSAFAVSQSNVGVSDLSLLDPILEKVQITMIEG
>Mature_312_residues
ASVVTITLNAAYDLVGRLNRIQLGEVNTVETLGLFPAGKGINVAKVLKDLGVNVAVGGFLGKDNSADFEQMFNQHGLEDK
FHRVDGKTRINVKITETEADVTDLNFLGYQISPQVWQQFVTDSLAYCLNYDIVAVCGSLPRGVSPELFADWLNQLHQAGV
KVVLDSSNAALTAGLKAKPWLVKPNHRELEAWVGHPLNSLEEIIAAAQQLKAEGIENVIISMGAKGSLWINNEGVLKAEP
AQCENVVSTVGAGDSMVAGLIYGFEKGLSKTETLAFATAVSAFAVSQSNVGVSDLSLLDPILEKVQITMIEG

Specific function: Unknown

COG id: COG1105

COG function: function code G; Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family

Homologues:

Organism=Escherichia coli, GI1788493, Length=305, Percent_Identity=55.4098360655738, Blast_Score=326, Evalue=1e-90,
Organism=Escherichia coli, GI1788017, Length=260, Percent_Identity=29.2307692307692, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): K1PF_HAEIN (P44330)

Other databases:

- EMBL:   L42023
- PIR:   A64069
- RefSeq:   NP_438608.1
- ProteinModelPortal:   P44330
- SMR:   P44330
- GeneID:   949542
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0447
- NMPDR:   fig|71421.1.peg.421
- TIGR:   HI_0447
- HOGENOM:   HBG670529
- OMA:   GIDNTAT
- ProtClustDB:   PRK09513
- BioCyc:   HINF71421:HI_0447-MONOMER
- BRENDA:   2.7.1.56
- InterPro:   IPR022463
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR017583
- TIGRFAMs:   TIGR03168
- TIGRFAMs:   TIGR03828

Pfam domain/function: PF00294 PfkB

EC number: =2.7.1.56

Molecular weight: Translated: 33523; Mature: 33391

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00583 PFKB_KINASES_1; PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASVVTITLNAAYDLVGRLNRIQLGEVNTVETLGLFPAGKGINVAKVLKDLGVNVAVGGF
CCEEEEEEECHHHHHHHCCCCEEECCCCCCHHEECCCCCCCCCHHHHHHHCCCCEEEECC
LGKDNSADFEQMFNQHGLEDKFHRVDGKTRINVKITETEADVTDLNFLGYQISPQVWQQF
CCCCCCCHHHHHHHHCCCCHHHHHCCCCEEEEEEEECCCCCCCHHEEECEEECHHHHHHH
VTDSLAYCLNYDIVAVCGSLPRGVSPELFADWLNQLHQAGVKVVLDSSNAALTAGLKAKP
HHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCEEEECCCCCC
WLVKPNHRELEAWVGHPLNSLEEIIAAAQQLKAEGIENVIISMGAKGSLWINNEGVLKAE
EEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCEEEECCCCCEECC
PAQCENVVSTVGAGDSMVAGLIYGFEKGLSKTETLAFATAVSAFAVSQSNVGVSDLSLLD
CHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
PILEKVQITMIEG
HHHHHEEEEEECC
>Mature Secondary Structure 
ASVVTITLNAAYDLVGRLNRIQLGEVNTVETLGLFPAGKGINVAKVLKDLGVNVAVGGF
CEEEEEEECHHHHHHHCCCCEEECCCCCCHHEECCCCCCCCCHHHHHHHCCCCEEEECC
LGKDNSADFEQMFNQHGLEDKFHRVDGKTRINVKITETEADVTDLNFLGYQISPQVWQQF
CCCCCCCHHHHHHHHCCCCHHHHHCCCCEEEEEEEECCCCCCCHHEEECEEECHHHHHHH
VTDSLAYCLNYDIVAVCGSLPRGVSPELFADWLNQLHQAGVKVVLDSSNAALTAGLKAKP
HHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCEEEECCCCCC
WLVKPNHRELEAWVGHPLNSLEEIIAAAQQLKAEGIENVIISMGAKGSLWINNEGVLKAE
EEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCEEEECCCCCEECC
PAQCENVVSTVGAGDSMVAGLIYGFEKGLSKTETLAFATAVSAFAVSQSNVGVSDLSLLD
CHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
PILEKVQITMIEG
HHHHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800