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The map label for this gene is fruB

Identifier: 16272396

GI number: 16272396

Start: 470880

End: 472379

Strand: Reverse

Name: fruB

Synonym: HI0448

Alternate gene names: 16272396

Gene position: 472379-470880 (Counterclockwise)

Preceding gene: 16272397

Following gene: 16272395

Centisome position: 25.81

GC content: 41.87

Gene sequence:

>1500_bases
ATGTTAGAACTTTCGGAAAGCAACATTCATTTAAATGCTAATGCAATTGATAAACAACAAGCGATCGAAATGGCAGTGTC
CGCATTGGTTCAAGCAGGCAATGTTGAAAATGGTTACTTGCAAGGAATGTTAGCGCGCGAGCTGCAAACCTCGACCTTTT
TAGGCAATGGCATCGCTATTCCTCACGGCACCTTGGATACTCGTTTGATGGTAAAGAAAACGGGCGTGCAAGTGTTTCAA
TTTCCTCAAGGCATAGAATGGGGCGAAGGAAATATTGCTTATGTCGTGATTGGTATTGCGGCGCGATCTGATGAGCATTT
GTCCTTATTACGTCAACTTACCCACGTTTTGAGTGATGAAGATACCGCAGCAAAATTAGCAAAAATAACTGATGTGGCTG
AATTTTGCGCGATTTTAATGGGCGAGACAATTGATCCATTTGAAATTCCTGCTGCCAATATCAGTTTAGATGTAAATACC
CAAAGTTTATTAACTTTAGTTGCGATTAATGCGGGGCAATTACAGGTGCAAAGTGCGGTAGAAAATCGCTTTATTTCTGA
AGTGATAAATAATGCGGCATTACCGCTTGGCAAAGGGTTATGGGTAACGGACTCTGTCGTAGGAAATGTGAAAAATGCCT
TAGCATTTAGTCGTGCTAAAACAATTTTTAGCCACAATGGCAAGGCCGTAAAAGGCGTGATAACCGTTTCAGCCGTCGGT
GATCAAATTAATCCAACTTTGGTGCGTTTATTAGATGACGATGTCCAAACAACCTTGCTCAATGGAAATTCAACAGAAAT
TTTGACCGCACTTTTGGGTTCATCGAGTGATGTTGAAACTCAATCTGTAGAAGGTGCTGTTGTAGGAACTTTTACAATTC
GTAATGAACACGGCTTACATGCACGCCCAAGTGCAAATTTGGTTAATGAAGTGAAAAAATTCACTTCTAAAATAACCATG
CAAAATCTTACTCGTGAAAGTGAAGTCGTCAGCGCAAAAAGTTTGATGAAGATTGTTGCGCTTGGTGTAACACAAGGACA
TCGTTTACGTTTTGTGGCAGAGGGGGAAGATGCAAAACAAGCAATTGAATCATTGGGCAAAGCGATAGCCAATGGCTTGG
GAGAGAATGTTTCGGCAGTGCCACCGTCTGAACCCGATACCATTGAGATTATGGGCGATCAAATTCACACACCTGCGGTA
ACAGAAGATGATAATTTGCCAGCAAATGCCATTGAAGCCGTATTTGTGATTAAAAACGAGCAAGGTTTGCACGCTCGCCC
AAGTGCAATATTGGTAAACGAAGTGAAGAAATATAATGCTTCCGTAGCAGTTCAAAACCTTGATCGTAATTCTCAATTAG
TCAGTGCTAAAAGTTTGATGAAAATTGTGGCATTAGGCGTAGTCAAAGGGACTCGTTTACGTTTTGTTGCGACGGGCGAA
GAAGCGCAACAAGCTATTGACGGAATCGGTGCTGTGATCGAATCGGGTTTAGGGGAATAG

Upstream 100 bases:

>100_bases
CGCTGCTGAAAGCCTGTACATCTAAATAATTGTTTTTCAATAAGCTGCTGGTCGATTTGTTTCATCCGCAGCTTTCTTTT
TAAATTATTAAGGAGCAAGA

Downstream 100 bases:

>100_bases
TATGGCAAGCGTCGTAACCATTACCTTAAATGCCGCCTATGATTTAGTTGGGCGTTTAAATCGTATTCAACTTGGCGAAG
TAAATACGGTAGAAACATTG

Product: bifunctional PTS system fructose-specific transporter subunit IIA/HPr protein

Products: protein histidine; sugar phosphate

Alternate protein names: MTP; Fructose PTS diphosphoryl transfer protein; Phosphotransferase FPr protein; Pseudo-HPr; Phosphocarrier protein HPr; Protein H; Fructose-specific phosphotransferase enzyme IIA component; EIIA-Fru; EIII-Fru; PTS system fructose-specific EIIA component

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MLELSESNIHLNANAIDKQQAIEMAVSALVQAGNVENGYLQGMLARELQTSTFLGNGIAIPHGTLDTRLMVKKTGVQVFQ
FPQGIEWGEGNIAYVVIGIAARSDEHLSLLRQLTHVLSDEDTAAKLAKITDVAEFCAILMGETIDPFEIPAANISLDVNT
QSLLTLVAINAGQLQVQSAVENRFISEVINNAALPLGKGLWVTDSVVGNVKNALAFSRAKTIFSHNGKAVKGVITVSAVG
DQINPTLVRLLDDDVQTTLLNGNSTEILTALLGSSSDVETQSVEGAVVGTFTIRNEHGLHARPSANLVNEVKKFTSKITM
QNLTRESEVVSAKSLMKIVALGVTQGHRLRFVAEGEDAKQAIESLGKAIANGLGENVSAVPPSEPDTIEIMGDQIHTPAV
TEDDNLPANAIEAVFVIKNEQGLHARPSAILVNEVKKYNASVAVQNLDRNSQLVSAKSLMKIVALGVVKGTRLRFVATGE
EAQQAIDGIGAVIESGLGE

Sequences:

>Translated_499_residues
MLELSESNIHLNANAIDKQQAIEMAVSALVQAGNVENGYLQGMLARELQTSTFLGNGIAIPHGTLDTRLMVKKTGVQVFQ
FPQGIEWGEGNIAYVVIGIAARSDEHLSLLRQLTHVLSDEDTAAKLAKITDVAEFCAILMGETIDPFEIPAANISLDVNT
QSLLTLVAINAGQLQVQSAVENRFISEVINNAALPLGKGLWVTDSVVGNVKNALAFSRAKTIFSHNGKAVKGVITVSAVG
DQINPTLVRLLDDDVQTTLLNGNSTEILTALLGSSSDVETQSVEGAVVGTFTIRNEHGLHARPSANLVNEVKKFTSKITM
QNLTRESEVVSAKSLMKIVALGVTQGHRLRFVAEGEDAKQAIESLGKAIANGLGENVSAVPPSEPDTIEIMGDQIHTPAV
TEDDNLPANAIEAVFVIKNEQGLHARPSAILVNEVKKYNASVAVQNLDRNSQLVSAKSLMKIVALGVVKGTRLRFVATGE
EAQQAIDGIGAVIESGLGE
>Mature_499_residues
MLELSESNIHLNANAIDKQQAIEMAVSALVQAGNVENGYLQGMLARELQTSTFLGNGIAIPHGTLDTRLMVKKTGVQVFQ
FPQGIEWGEGNIAYVVIGIAARSDEHLSLLRQLTHVLSDEDTAAKLAKITDVAEFCAILMGETIDPFEIPAANISLDVNT
QSLLTLVAINAGQLQVQSAVENRFISEVINNAALPLGKGLWVTDSVVGNVKNALAFSRAKTIFSHNGKAVKGVITVSAVG
DQINPTLVRLLDDDVQTTLLNGNSTEILTALLGSSSDVETQSVEGAVVGTFTIRNEHGLHARPSANLVNEVKKFTSKITM
QNLTRESEVVSAKSLMKIVALGVTQGHRLRFVAEGEDAKQAIESLGKAIANGLGENVSAVPPSEPDTIEIMGDQIHTPAV
TEDDNLPANAIEAVFVIKNEQGLHARPSAILVNEVKKYNASVAVQNLDRNSQLVSAKSLMKIVALGVVKGTRLRFVATGE
EAQQAIDGIGAVIESGLGE

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG4668

COG function: function code G; Mannitol/fructose-specific phosphotransferase system, IIA domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain

Homologues:

Organism=Escherichia coli, GI1788494, Length=383, Percent_Identity=49.6083550913838, Blast_Score=347, Evalue=1e-96,
Organism=Escherichia coli, GI1790027, Length=144, Percent_Identity=43.75, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1788755, Length=79, Percent_Identity=48.1012658227848, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTFAH_HAEIN (P44715)

Other databases:

- EMBL:   L42023
- PIR:   B64069
- RefSeq:   NP_438609.1
- ProteinModelPortal:   P44715
- GeneID:   950651
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0448
- NMPDR:   fig|71421.1.peg.422
- TIGR:   HI_0448
- HOGENOM:   HBG511960
- OMA:   AIPHGTL
- ProtClustDB:   PRK13779
- BioCyc:   HINF71421:HI_0448-MONOMER
- GO:   GO:0005737
- InterPro:   IPR016258
- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114
- Gene3D:   G3DSA:3.40.930.10
- Gene3D:   G3DSA:3.30.1340.10
- PIRSF:   PIRSF000690
- PRINTS:   PR00107
- TIGRFAMs:   TIGR01003

Pfam domain/function: PF00381 PTS-HPr; PF00359 PTS_EIIA_2; SSF55594 HPr_protein; SSF55804 PTrfase/Anion_transptr

EC number: 2.7.1.69

Molecular weight: Translated: 53008; Mature: 53008

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS51094 PTS_EIIA_TYPE_2; PS00372 PTS_EIIA_TYPE_2_HIS; PS51350 PTS_HPR_DOM; PS00369 PTS_HPR_HIS; PS00589 PTS_HPR_SER

Important sites: ACT_SITE 62-62 ACT_SITE 300-300 ACT_SITE 424-424

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLELSESNIHLNANAIDKQQAIEMAVSALVQAGNVENGYLQGMLARELQTSTFLGNGIAI
CCEECCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEC
PHGTLDTRLMVKKTGVQVFQFPQGIEWGEGNIAYVVIGIAARSDEHLSLLRQLTHVLSDE
CCCCCCCEEEEEECCCEEEECCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHCCC
DTAAKLAKITDVAEFCAILMGETIDPFEIPAANISLDVNTQSLLTLVAINAGQLQVQSAV
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHEEEEEEECCCCCHHHHHH
ENRFISEVINNAALPLGKGLWVTDSVVGNVKNALAFSRAKTIFSHNGKAVKGVITVSAVG
HHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECC
DQINPTLVRLLDDDVQTTLLNGNSTEILTALLGSSSDVETQSVEGAVVGTFTIRNEHGLH
CCCCHHHHHHHCCCCCEEEECCCCHHHHHHHHCCCCCCCHHCCCCEEEEEEEEECCCCCC
ARPSANLVNEVKKFTSKITMQNLTRESEVVSAKSLMKIVALGVTQGHRLRFVAEGEDAKQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHH
AIESLGKAIANGLGENVSAVPPSEPDTIEIMGDQIHTPAVTEDDNLPANAIEAVFVIKNE
HHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEECC
QGLHARPSAILVNEVKKYNASVAVQNLDRNSQLVSAKSLMKIVALGVVKGTRLRFVATGE
CCCCCCCCHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCH
EAQQAIDGIGAVIESGLGE
HHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLELSESNIHLNANAIDKQQAIEMAVSALVQAGNVENGYLQGMLARELQTSTFLGNGIAI
CCEECCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEC
PHGTLDTRLMVKKTGVQVFQFPQGIEWGEGNIAYVVIGIAARSDEHLSLLRQLTHVLSDE
CCCCCCCEEEEEECCCEEEECCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHCCC
DTAAKLAKITDVAEFCAILMGETIDPFEIPAANISLDVNTQSLLTLVAINAGQLQVQSAV
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHEEEEEEECCCCCHHHHHH
ENRFISEVINNAALPLGKGLWVTDSVVGNVKNALAFSRAKTIFSHNGKAVKGVITVSAVG
HHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECC
DQINPTLVRLLDDDVQTTLLNGNSTEILTALLGSSSDVETQSVEGAVVGTFTIRNEHGLH
CCCCHHHHHHHCCCCCEEEECCCCHHHHHHHHCCCCCCCHHCCCCEEEEEEEEECCCCCC
ARPSANLVNEVKKFTSKITMQNLTRESEVVSAKSLMKIVALGVTQGHRLRFVAEGEDAKQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHH
AIESLGKAIANGLGENVSAVPPSEPDTIEIMGDQIHTPAVTEDDNLPANAIEAVFVIKNE
HHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEECC
QGLHARPSAILVNEVKKYNASVAVQNLDRNSQLVSAKSLMKIVALGVVKGTRLRFVATGE
CCCCCCCCHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCH
EAQQAIDGIGAVIESGLGE
HHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800; 8763608